Article
Chemistry, Physical
Ahmet Yildirim, Mustafa Tekpinar, Tsjerk A. Wassenaar
Summary: The study reveals that Ca2+ binding enhances the dynamics of S100A4, regulating its interaction with NMIIA. NMIIA induces asymmetric dynamics between the chains of S100A4. In the absence of Ca2+, the interaction between S100A4 and NMIIA is weak.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Clinical Neurology
Shuaishuai Gong, Guosheng Cao, Fang Li, Zhuo Chen, Xuewei Pan, Huifen Ma, Yuanyuan Zhang, Boyang Yu, Junping Kou
Summary: The study suggests that NMMHC IIA plays a crucial role in ischemic stroke by maintaining the integrity of the cerebral endothelial barrier, making it a potential therapeutic target for ischemic stroke.
Article
Cell Biology
Kai Weissenbruch, Magdalena Fladung, Justin Grewe, Laurent Baulesch, Ulrich S. Schwarz, Martin Bastmeyer
Summary: Nonmuscle myosin II minifilaments are important for force generation and mechanosensing in mammalian cells. Different minifilaments have different compositions and activities, with a strong asymmetry between paralogs A and B. NM IIA is responsible for the formation of new minifilaments, while NM IIB acts as a regulator to prevent excessive activity. These two paralogs work together to form a balanced system.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Madoca Inukai, Ako Yokoi, Yuuki Ishizuka, Miki Hashimura, Toshihide Matsumoto, Yasuko Oguri, Mayu Nakagawa, Yu Ishibashi, Takashi Ito, Toshihiro Kumabe, Makoto Saegusa
Summary: The S100A4/NMIIA axis plays an important role in the tumorigenesis and vasculogenesis of glioblastoma (GBM). S100A4(+)/HIF-1α(-) GBM cells are recruited to preexisting vessels by inhibiting NMIIA activity. These events lead to tumor progression through the release of extracellular VEGF.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Fang Zhang, Yong Li, Lipeng Gan, Xiaomei Tong, Dandan Qi, Qihui Wang, Xin Ye
Summary: The study identified lncRNA MAFG-AS1 as upregulated in HBV-related HCC tissues, leading to enhanced proliferation and migration of HCC cells, suggesting its potential oncogenic role in HBV-related HCC development.
Article
Virology
Jian Chen, Jian Liu, Zhilu Chen, Daobin Feng, Cuisong Zhu, Jun Fan, Shuye Zhang, Xiaoyan Zhang, Jianqing Xu
Summary: This study reveals the crucial role of nonmuscle myosin IIA (MYH9) in regulating influenza A virus (IAV) infection and replication. MYH9 can interact with the viral nucleoprotein (NP) and interrupt the formation of viral ribonucleoprotein (vRNP) complexes, leading to a suppression of viral RNA transcription and replication. These findings shed new light on the complicated interactions between IAV and its host, making MYH9 a potential target for antiviral drug development.
Article
Biochemistry & Molecular Biology
Li Zhu, Dylan Johnson, Joseph M. Chalovich
Summary: The C-terminal 14-16 residues of human troponin T are crucial for full inactivation and prevent full activation at saturating Ca2+. The basic residues within this region are essential for the function of TnT, but the mechanism of action is still unknown.
Article
Physiology
Sarah R. Clippinger, Paige E. Cloonan, Wei Wang, Lina Greenberg, W. Tom Stump, Paweorn Angsutararux, Jeanne M. Nerbonne, Michael J. Greenberg
Summary: Hypertrophic cardiomyopathy (HCM) is primarily caused by mutations in sarcomeric proteins and is the leading cause of sudden cardiac death in individuals under 30 years of age. The complex pathogenesis of HCM involves changes in molecular, cellular, and tissue levels of function. Research suggests that molecularly driven changes in mechanical tension are the primary driver of early disease progression in familial HCM.
JOURNAL OF GENERAL PHYSIOLOGY
(2021)
Article
Oncology
Christina Parkes, Areege Kamal, Anthony J. Valentijn, Rafah Alnafakh, Stephane R. Gross, Roger Barraclough, Diana Moss, John Kirwan, Dharani K. Hapangama
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
(2018)
Article
Cell Biology
Stephen T. Hallett, Martyna W. Pastok, R. Marc L. Morgan, Anita Wittner, Katie L. I. M. Blundell, Ildiko Felletar, Stephen R. Wedge, Chrisostomos Prodromou, Martin E. M. Noble, Laurence H. Pearl, Jane A. Endicott
Article
Biochemistry & Molecular Biology
Alessandro Annibaldi, Sidonie Wicky John, Tom Vanden Berghe, Kirby N. Swatek, Jianbin Ruan, Gianmaria Liccardi, Katiuscia Bianchi, Paul R. Elliott, Sze Men Choi, Samya Van Coillie, John Bertin, Hao Wu, David Komander, Peter Vandenabeele, John Silke, Pascal Meier
Article
Biochemistry & Molecular Biology
Andrew C. McShan, Kannan Natarajan, Vlad K. Kumirov, David Flores-Solis, Jiansheng Jiang, Mareike Badstubner, Jugmohit S. Toor, Clive R. Bagshaw, Evgenii L. Kovrigin, David H. Margulies, Nikolaos G. Sgourakis
NATURE CHEMICAL BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Abdullah Y. Mandourah, Lakshminarayan Ranganath, Roger Barraclough, Sobhan Vinjamuri, Robert Van'T Hof, Sandra Hamill, Gabriela Czanner, Ayed A. Dera, Duolao Wang, Dong L. Barraclough
SCIENTIFIC REPORTS
(2018)
Article
Cell Biology
Rebecca Feltham, Kunzah Jamal, Tencho Tenev, Gianmaria Liccardi, Isabel Jaco, Celia Monteiro Domingues, Otto Morris, Sidonie Wicky John, Alessandro Annibaldi, Marcella Widya, Conor J. Kearney, Danielle Clancy, Paul R. Elliott, Timo Glatter, Qi Qiao, Andrew J. Thompson, Alexey Nesvizhskii, Alexander Schmidt, David Komander, Hao Wu, Seamus Martin, Pascal Meier
Article
Multidisciplinary Sciences
Linnea I. Jansson, Jendrik Hentschel, Joseph W. Parks, Terren R. Chang, Cheng Lu, Rishika Baral, Clive R. Bagshaw, Michael D. Stone
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Multidisciplinary Sciences
Ayed A. Dera, Lakshminarayan Ranganath, Roger Barraclough, Sobhan Vinjamuri, Sandra Hamill, Dong L. Barraclough
SCIENTIFIC REPORTS
(2019)
Article
Cell Biology
Michela Serena, Ricardo Nunes Bastos, Paul R. Elliott, Francis A. Barr
JOURNAL OF CELL BIOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Amelia B. Labbe, Clive R. Bagshaw, Lisa Uttal
JOURNAL OF CHEMICAL EDUCATION
(2020)
Article
Biochemistry & Molecular Biology
Marco Salamina, Bailey C. Montefiore, Mengxi Liu, Daniel J. Wood, Richard Heath, James R. Ault, Lan-Zhen Wang, Svitlana Korolchuk, Arnaud Basle, Martyna W. Pastok, Judith Reeks, Natalie J. Tatum, Frank Sobott, Stefan T. Arold, Michele Pagano, Martin E. M. Noble, Jane A. Endicott
Summary: The SCFSKP2 ubiquitin ligase promotes degradation of p27KIP1 to relieve G1 checkpoint control of CDK-cyclin complexes, ensuring orderly cell cycle progression. The interaction between SKP2 and cyclin A provides a structural mechanism for fine-tuning the degradation of p27KIP1 and distinguishes cyclin A from other G1 cyclins.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Clive R. Bagshaw, Jendrik Hentschel, Michael D. Stone
Summary: Telomerases are moderately processive reverse transcriptases that extend the 3' ends of linear chromosomes using an integral RNA template. The processivity values vary at different positions, with a characteristic strong band every 6th position in gel electrophoresis due to repeat nucleotides. This analysis reveals that higher product concentrations associated with a certain type of processivity arise from a stalling of nucleotide incorporation during translocation.
Article
Multidisciplinary Sciences
Archana G. Chavan, Jeffrey A. Swan, Joel Heisler, Cigdem Sancar, Dustin C. Ernst, Mingxu Fang, Joseph G. Palacios, Rebecca K. Spangler, Clive R. Bagshaw, Sarvind Tripathi, Priya Crosby, Susan S. Golden, Carrie L. Partch, Andy LiWang
Summary: The study successfully reconstructed a complete cyanobacterial circadian clock system in vitro, including the central oscillator, signal transduction pathways, downstream transcription factor, and promoter DNA. By monitoring the oscillations in real time with a fluorescence-based readout, the study revealed previously unknown mechanisms by which the circadian system of cyanobacteria maintains pace and rhythmicity under variable protein concentrations.
Article
Multidisciplinary Sciences
Larissa Dietz, Cara J. Ellison, Carlos Riechmann, C. Keith Cassidy, F. Daniel Felfoldi, Adan Pinto-Fernandez, Benedikt M. Kessler, Paul R. Elliott
Summary: Certain members of the inhibitor of apoptosis (IAP) family, including BIRC6, regulate cell death by inhibiting caspases, while SMAC antagonizes IAPs and promotes cell death. BIRC6 directly inhibits caspase-3 and-7 and ubiquitinates caspase-3,-7, and-9, working with noncanonical E1, UBA6. SMAC suppresses both mechanisms by competitively displacing caspases.
Meeting Abstract
Immunology
Nikolaos Sgourakis, Andrew McShan, Kannan Natarajan, Jiansheng Jiang, Jihye Park, Sarah Overall, Jugmohit S. Toor, Vlad Kumirov, David Flores-Solis, Mareike Badstubner, Evgenii L. Kovrigin, Clive R. Bagshaw, Jesper Pallesen, Erik Procko, David H. Margulies
JOURNAL OF IMMUNOLOGY
(2019)