Journal
STRUCTURE
Volume 19, Issue 9, Pages 1219-1232Publisher
CELL PRESS
DOI: 10.1016/j.str.2011.05.014
Keywords
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Funding
- New York Structural Biology Center
- NIH [R01 GM56960, U54 GM094598, DP2 OD004631]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0741914] Funding Source: National Science Foundation
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CopA uses ATP to pump Cu+ across cell membranes. X-ray crystallography has defined atomic structures of several related P-type ATPases. We have determined a structure of CopA at 10 angstrom resolution by cryo-electron microscopy of a new crystal form and used computational molecular docking to study the interactions between the N-terminal metal-binding domain (NMBD) and other elements of the molecule. We found that the shorter-chain lipids used to produce these crystals are associated with movements of the cytoplasmic domains, with a novel dimer interface and with disordering of the NMBD, thus offering evidence for the transience of its interaction with the other cytoplasmic domains. Docking identified a binding site that matched the location of the NMBD in our previous structure by cryo-electron microscopy, allowing a more detailed view of its binding configuration and further support for its role in autoinhibition.
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