Article
Cell Biology
Gota Yoshida, Tsuyoshi Kawabata, Hyota Takamatsu, Shotaro Saita, Shuhei Nakamura, Keizo Nishikawa, Mari Fujiwara, Yusuke Enokidani, Tadashi Yamamuro, Keisuke Tabata, Maho Hamasaki, Masaru Ishii, Atsushi Kumanogoh, Tamotsu Yoshimori
Summary: This study found that upregulation of autophagy in osteoblasts promotes bone formation and deficiency in autophagy disrupts bone homeostasis. It identified RUBCN as a key regulator of bone homeostasis and suggested that targeting RUBCN or autophagic degradation of NICD could be used as potential treatments for osteoporosis and bone fractures.
Article
Biochemistry & Molecular Biology
Kyung-Ran Park, Joon Yeop Lee, Myounglae Cho, Jin Tae Hong, Hyung-Mun Yun
Summary: Paeonoside (PASI) isolated from Paeonia suffruticosa roots enhances osteoblast migration, early differentiation, and mineralized nodule formation by regulating RUNX2 expression through the BMP2 and Wnt3a pathways, suggesting a potential therapeutic role in treating bone diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Dandan Song, Huan He, Rajitha Indukuri, Zhiqiang Huang, Lina Stepanauskaite, Indranil Sinha, Lars-Arne Haldosen, Chunyan Zhao, Cecilia Williams
Summary: This study investigates the differences between estrogen receptor alpha (ERα) and estrogen receptor beta (ERβ) in regulating cell proliferation and migration. The results show that ERα and ERβ have opposite effects on cell proliferation and different impacts on migration. Furthermore, they regulate distinct sets of target genes, providing valuable insights into the mechanisms of these two different ER isoforms.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Chemistry, Medicinal
Ka-Ying Wong, Tsz-Hung Kong, Christina Chui-Wa Poon, Wenxuan Yu, Liping Zhou, Man-Sau Wong
Summary: In this study, the role of ER-α66, ER-α36, and GPER in bone metabolism following icariin treatment was investigated. It was found that icariin regulated ER-α36 and GPER protein expression in osteoblasts by downregulating them and upregulating ER-α66. ER-α36 and GPER acted as negative regulators of ER-α66 in intact osteoblasts.
PHYTOTHERAPY RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Yuma Nihashi, Mana Miyoshi, Koji Umezawa, Takeshi Shimosato, Tomohide Takaya
Summary: The study identified an 18-base oligodeoxynucleotide (ODN) called iSN40 that enhances the activity and gene expression of osteoblasts, promoting their differentiation and calcium deposition. iSN40 may serve as a potential nucleic acid drug for osteoporosis therapy.
Article
Agriculture, Multidisciplinary
Gong-Jhe Wu, Kung-Yen Chen, Jr-Di Yang, Shing-Hwa Liu, Ruei-Ming Chen
Summary: The study showed that naringin, a citrus flavonoid, can enhance bone mineralization and healing in osteoporosis-related bone diseases by activating ERα and ALP expression.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Nikola Stokovic, Natalia Ivanjko, Igor Erjavec, Anita Breski, Mihaela Peric, Slobodan Vukicevic
Summary: The study found that adding Zoledronate (ZOL) to autologous bone graft substitute (ABGS) enhances the quality and quantity of newly formed bone, and also prolongs its longevity to a certain extent.
Article
Cell Biology
Yue Hu, Xinqing Hao, Cangwei Liu, Chunxia Ren, Shuangshuang Wang, Guangxing Yan, Yuan Meng, Yuji Mishina, Ce Shi, Hongchen Sun
Summary: The study showed that deletion of Acvr1 in the mandible leads to bone loss, mainly due to compromised osteoblast differentiation and enhanced osteoclastogenesis. In vitro experiments demonstrated that Acvr1 deficiency in mandibular bone marrow stromal cells resulted in increased proliferation and differentiation of osteoclasts. Additionally, the increased osteoclastogenesis may be partially attributed to the secretion of sRANKL.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Yanjie Wang, Jinghe Sun, Yanqi Zhang, Wenzhuan Liu, Sheng Yang, Jingfeng Yang
Summary: The combined use of SP-2 and BMP-2 enhanced osteoblastic differentiation by increasing ALP expression and mineralization speed, leading to higher levels of BMP-2, Runx-2, COL-I, OCN, and OPN expression. SP-2 acted as a more effective enhancer by increasing the binding efficiency between BMP-2 and its cell surface receptor, proving to be most effective at a dose of 5 μg/mL.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Plant Sciences
Ming-Shi Ren, Hui-Hui Xie, Yu Ding, Zi-Han Li, Bo Liu
Summary: This study aimed to determine whether the serum containing EXD promotes the proliferation and differentiation of osteoblasts through the regulation of BK channel.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Binisha H. Mishra, Pashupati P. Mishra, Emma Raitoharju, Saara Marttila, Nina Mononen, Harri Sievanen, Jorma Viikari, Markus Juonala, Marika Laaksonen, Nina Hutri-Kahonen, Mika Kahonen, Olli T. Raitakari, Terho Lehtimaki
Summary: This study analysed gene co-expression modules shared by early traits of osteoporosis and atherosclerosis, identifying two modules with significant joint association with both diseases. The significant member genes from these modules are involved in biological processes related to bone metabolism or atherosclerosis, potentially providing new joint biomarkers for both diseases.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Benjamin Tajer, James A. Dutko, Shawn C. Little, Mary C. Mullins
Summary: The study explores the BMP2/7 heterodimer signaling mechanism in zebrafish embryos, revealing that BMP2 homodimers require nonredundant type I receptors Acvr1 and Bmpr1 for signaling, despite not being able to bind Acvr1 in vitro. The kinase activity of Acvr1 is essential for the signaling complex, suggesting that the potency of the heterodimer arises from recruiting both Acvr1 and Bmpr1.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Environmental Sciences
Emilie Farcy, Eric Potier, Nicolas Leurs, Eric Gasset, Gilbert Dutto, Stephane Lallement, Clarence Bourdy, Melanie Debiais-Thibaud, Camille Martinand-Mari
Summary: This study found that early exposure to (xeno)estrogens can increase the expression of skeleton-associated genes in fish, but only natural estrogen E2 leads to increased mineralization. This suggests that the effects of (xeno)estrogens on early skeletal development in fish may vary.
FRONTIERS IN MARINE SCIENCE
(2022)
Review
Immunology
Tatjana Bordukalo-Niksic, Vera Kufner, Slobodan Vukicevic
Summary: Bone remodeling in the adult skeleton involves a continuous process of old and damaged bone removal by osteoclasts and subsequent new bone formation by osteoblasts. BMPs play a crucial role in maintaining healthy bone, affecting both bone formation and resorption, and may be involved in pathological processes related to inflammatory disorders affecting bones and joints.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Liwei Zhu, Yuzhe Liu, Ao Wang, Zhengqing Zhu, Youbin Li, Chenyi Zhu, Zhenjia Che, Tengyue Liu, He Liu, Lanfeng Huang
Summary: Bone morphogenetic protein (BMP) plays an important role in promoting bone healing, but its mechanism needs further exploration. By modifying implants to carry BMP and achieve slow and sustained release, effective bone healing can be stimulated.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Rheumatology
Ken-ei Sada, Yoshiki Kojo, Jolyon Fairburn-Beech, Keiko Sato, Shoko Akiyama, Melissa K. Van Dyke, Isao Mukai
Summary: This study aimed to estimate the prevalence and disease burden of eosinophilic granulomatosis with polyangiitis (EGPA) in Japan. The results showed an increasing prevalence of EGPA over time, with a higher incidence in females and older patients. EGPA patients often experience respiratory comorbidities, requiring hospitalization and oral corticosteroid use.
MODERN RHEUMATOLOGY
(2022)
Article
Virology
Atsushi Miyauchi, Ken-Ei Sada, Hirotaka Yamamoto, Hiroki Iriyoshi, Yuji Touyama, Daisuke Hashimoto, Shigeru Nojima, Shingo Yamanaka, Keita Ishijima, Ken Maeda, Masafumi Kawamura
Summary: A 67-year-old male veterinarian presented with fatigue, anorexia, and diarrhea. He was diagnosed with severe fever with thrombocytopenia syndrome (SFTS) based on bicytopenia, mild disturbance of consciousness, and a history of outdoor activities. Immunoglobulin therapy was immediately started and a serum reverse transcription-polymerase chain reaction (RT-PCR) test confirmed the presence of SFTS virus (SFTSV). The patient had contact with a thrombocytopenic cat before admission, and both the patient and cat had identical SFTSV genome sequences, indicating a possible transmission from the cat to the patient. Direct cat-to-human transmission of SFTV has been reported, emphasizing the need for mucous membrane protection when in contact with cats suspected of having SFTS.
Article
Multidisciplinary Sciences
Daisuke Waki, Hiroya Tamai, Ritsuko Yokochi, Toshiki Kido, Yuriko Yagyu, Ryo Yanai, Ken-Ei Sada
Summary: This study compared the clinical response to methotrexate between anti-SSA antibody-positive and -negative patients with methotrexate-naive rheumatoid arthritis and found that anti-SSA antibody-positive patients had a lower rate of achieving low disease activity after 6 months of methotrexate treatment. Residual pain may contribute to this reduced response.
Article
Multidisciplinary Sciences
Ken-ei Sada, Ryohei Yamamoto, Akihiko Yano, Atsushi Miyauchi, Masafumi Kawamura, Hideki Ito
Summary: Research suggests that monitoring bicarbonate concentrations in COVID-19 patients may be useful for predicting prognosis, with both high and low concentrations associated with a higher rate of clinical worsening.
Article
Rheumatology
Izaya Nakaya, Ken-ei Sada, Masayoshi Harigai, Jun Soma, Koichi Amano, Hiroaki Dobashi, Tatsuya Atsumi, Yukio Yuzawa, Shouichi Fujimoto, Takahiko Sugihara, Yoshinari Takasaki, Yoshihiro Arimura, Hirofumi Makino
Summary: This study investigated the current practice and effectiveness of prophylactic treatment against Pneumocystis jirovecii pneumonia (PCP) in patients with anti-neutrophil cytoplasmic antibody-associated vasculitis (AAV). The results showed that most patients received prophylactic drugs against PCP and more than 50% of them continued the prophylaxis after 2 years. The incidence rate of PCP was lower in patients who received chemoprophylaxis, but the effectiveness was not statistically confirmed.
MODERN RHEUMATOLOGY
(2022)
Article
Rheumatology
Yoshia Miyawaki, Takao Fujii, Keisuke Anan, Masanari Kodera, Masao Kikuchi, Ken-Ei Sada, Kenji Nagasaka, Masashi Bando, Hitoshi Sugiyama, Shinya Kaname, Masayoshi Harigai, Naoto Tamura
Summary: This study investigated the evidence-practice gap among physicians in Japan regarding the management of antineutrophil cytoplasmic antibody-associated vasculitis. The results showed significant variation in concordance among different subspecialties, highlighting the need for further research and promotional initiatives to address this gap in clinical practice.
MODERN RHEUMATOLOGY
(2023)
Article
Rheumatology
Ken-ei Sada, Yoshia Miyawaki, Kenta Shidahara, Shoichi Nawachi, Yu Katayama, Yosuke Asano, Keigo Hayashi, Keiji Ohashi, Eri Katsuyama, Takayuki Katsuyama, Mariko Takano-Narazaki, Yoshinori Matsumoto, Nao Oguro, Yuichi Ishikawa, Natsuki Sakurai, Chiharu Hidekawa, Ryusuke Yoshimi, Dai Kishida, Takanori Ichikawa, Yasuhiro Shimojima, Noriaki Kurita, Nobuyuki Yajima
Summary: Personality traits may influence the outcomes of patients with SLE. This study found a relationship between the grit of attending physicians and the achievement of treatment goals in SLE patients. Difficulty for physicians to change treatment goals might lead to a lower achievement of a lupus low disease activity state in patients with SLE.
Article
Rheumatology
Ken-ei Sada, Yu Katayama, Yosuke Asano, Keigo Hayashi, Yoshia Miyawaki, Keiji Ohashi, Eri Katsuyama, Takayuki Katsuyama, Mariko Takano-Narazaki, Yoshinori Matsumoto, Ryusuke Yoshimi, Yasuhiro Shimojima, Shigeru Ohno, Hiroshi Kajiyama, Kunihiro Ichinose, Shuzo Sato, Michio Fujiwara, Nobuyuki Yajima
Summary: The objective of this study was to explore the factors associated with GC-free treatment status in patients with SLE. The results showed that even in patients with long disease duration, GC-free treatment status might be related to no chronic damage accrual.
LUPUS SCIENCE & MEDICINE
(2022)
Article
Rheumatology
Kenei Sada, Noriaki Kurita, Hisashi Noma, Taizo Matsuki, Holly Quasny, Roger A. Levy, Angela R. Jones-Leone, Kerry Gairy, Nobuyuki Yajima
Summary: This study aims to evaluate the real-world effectiveness of belimumab plus standard therapy (ST) versus ST alone in patients with a history of active LN in the non-induction phase. The study will use postmarketing surveillance study data and the Lupus Registry of Nationwide Institutions (LUNA) data for observation and analysis.
LUPUS SCIENCE & MEDICINE
(2022)
Article
Rheumatology
Nao Oguro, Nobuyuki Yajima, Yoshia Miyawaki, Ryusuke Yoshimi, Yasuhiro Shimojima, Ken-ei Sada, Keigo Hayashi, Kenta Shidahara, Natsuki Sakurai, Chiharu Hidekawa, Dai Kishida, Takanori Ichikawa, Yuichi Ishikawa, Noriaki Kurita
Summary: This study examines how different aspects of health literacy (functional, communicative, and critical) affect patients' trust in their physicians. The results suggest that higher functional and communicative health literacy are associated with increased trust in one's physician, while higher critical health literacy is associated with decreased trust in physicians in general. The study also found that longer internet use is associated with higher communicative and critical health literacy.
JOURNAL OF RHEUMATOLOGY
(2023)
Article
Rheumatology
Ken-ei Sada, Shinya Kaname, Tomoaki Higuchi, Shunsuke Furuta, Kenji Nagasaka, Toshihiro Nanki, Naotake Tsuboi, Koichi Amano, Hiroaki Dobashi, Keiju Hiromura, Masashi Bando, Takashi Wada, Yoshihiro Arimura, Hirofumi Makino, Masayoshi Harigai
Summary: The study aimed to compare the American College of Rheumatology/European Alliance of Associations for Rheumatology 2022 criteria with the previous classification algorithm for anti-neutrophil cytoplasmic antibody-associated vasculitis. Data from two nationwide, prospective, inception cohort studies were used. The results showed that although the 2022 criteria had overlapping multiple criteria fulfillments in some patients, it helped in classifying unclassifiable patients into microscopic polyangiitis (MPA) using Watts' algorithm.
MODERN RHEUMATOLOGY
(2023)
Article
Rheumatology
Ken-ei Sada, Kenji Nagasaka, Shinya Kaname, Tomoaki Higuchi, Shunsuke Furuta, Toshihiro Nanki, Naotake Tsuboi, Koichi Amano, Hiroaki Dobashi, Keiju Hiromura, Masashi Bando, Takashi Wada, Yoshihiro Arimura, Hirofumi Makino, Masayoshi Harigai
Summary: This study aimed to compare the MHLW diagnostic criteria for antineutrophil cytoplasmic antibody-associated vasculitis with the new American College of Rheumatology/European Alliance of Associations for Rheumatology 2022 criteria. The results showed that a significant number of patients could be classified using the MHLW criteria, and the classification was in accordance with the American College of Rheumatology/European Alliance of Associations for Rheumatology 2022 criteria when considering the order of application.
MODERN RHEUMATOLOGY
(2023)
Article
Rheumatology
Ken-ei Sada, Kenji Nagasaka, Shinya Kaname, Eishu Nango, Kan Kishibe, Hiroaki Dobashi, Keiju Hiromura, Tamihiro Kawakami, Masashi Bando, Takashi Wada, Koichi Amano, Yohko Murakawa, Masayoshi Harigai
Summary: The objective of this study was to revise the 2017 clinical practice guidelines for the management of microscopic polyangiitis (MPA) and granulomatosis with polyangiitis (GPA). Based on the results, the recommendations have been updated, including the use of a combination therapy of glucocorticoid and cyclophosphamide or rituximab for remission induction. The study also suggests the use of avacopan and discourages the use of plasma exchange in severe cases of MPA/GPA.
MODERN RHEUMATOLOGY
(2023)
Article
Rheumatology
Ken-ei Sada, Takeo Suzuki, Sandra Joksaite, Shinyoung Ju, John Logie, George Mu, Jeremiah Hwee, Hideaki Kunishige, Takeo Ishii, Amit Adlak, Harini Vadlamudi, Rafael Alfonso-Cristancho
Summary: This study reports the prevalence of eosinophilic granulomatosis with polyangiitis (EGPA) and describes the use of oral corticosteroids (OCS) and the disease burden before and after the approval of mepolizumab in Japan. The results show that with the introduction of mepolizumab, real-world OCS use, relapses, and healthcare resource utilization decreased in patients with EGPA.
MODERN RHEUMATOLOGY
(2023)
Editorial Material
Rheumatology
Shigemi Morishita, Ken-ei Sada
ARTHRITIS & RHEUMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Olexander M. Semenenko, Victoria V. Lipson, Alina O. Sadchenko, Olga V. Vashchenko, Natalia A. Kasian, Liliia Sviechnikova, Longin M. Lisetski, Mykola L. Babak, Volodymyr M. Vakula, Oleksandr Borysov, Yuliia Holota, Sergey O. Zozulya, Petro O. Borysko, Olexander Mazepa
Summary: An efficient protocol for the synthesis of novel methotrexate-betulonic acid hybrids has been developed and their structures and properties have been fully established. The hybrids showed inferior interactions with artificial lipid membranes, possibly due to their increasing lipophilicity.
Article
Biochemistry & Molecular Biology
Mohadese Yaghoobi Anzabi, Piotr Cmoch, Roman Luboradzki, Zbigniew Pakulski
Summary: Friedelin and 3-acetoxyfriedel-3-en-2-one, known as friedelane triterpenoids, have been isolated from cork smoker wash solids and used as starting materials for the synthesis of new friedelane derivatives. Reduction reactions result in the formation of friedelinol and epi-cerin derivatives.
Article
Biochemistry & Molecular Biology
Detlef Thieme, Aniko Krumbholz, Martin Bidlingmaier, Christoph Geffert, Annika Hameder, Andreas Stoever, Matthias Graw, Annekathrin M. Keiler
Summary: Ethanol can affect testosterone metabolism and potentially influence steroid biosynthesis and blood steroid profiles. The study emphasizes the importance of sufficient time interval from food and ethanol intake during blood sampling, particularly for diagnostics in doping control.
Article
Biochemistry & Molecular Biology
Masanobu Murao, Tetsuo Imano, Yoshinobu Sato, Masaaki Nakajima
Article
Biochemistry & Molecular Biology
Jingyuan Wang, Ye Feng, Brian Liu, Wen Xie
Summary: Sulfation and desulfation of steroids are opposing processes that regulate steroid homeostasis and are involved in steroid-related diseases. Cytosolic sulfotransferase and steroid sulfatase modify and regulate steroids to affect hormonal dysregulation, metabolic diseases, and cancer.
Article
Biochemistry & Molecular Biology
Noah K. Babel, Brian J. Feldman
Summary: Our research identified a previously unrecognized ADAMTS1-PTN-Wnt pathway in regulating adipose precursor cell behavior. The non-muscle myosin protein MYH9 was found to be a key target of this pathway for modulating adipogenesis. These findings offer potential for developing novel therapeutics for obesity and related metabolic disorders.
Article
Biochemistry & Molecular Biology
Martiniano Bello
Summary: This study explores the structural and thermodynamic basis of molecular recognition between ERβ and DPN and its derivatives using molecular dynamics simulations and end-point methods. The results show that the MMGBSA approach is able to reproduce experimental trends and identify eight residues that strongly interact with the ligands.