Journal
STEROIDS
Volume 77, Issue 3, Pages 233-240Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.steroids.2011.11.010
Keywords
Dehydroepiandrosterone; Endothelial cells; High glucose; Inflammation; Oxidative stress
Funding
- CONACyT
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Dehydroepiandrosterone (DHEA), an adrenal steroid, has a protective role against diabetes: however, its mechanisms of action are unknown. Here, we focus on the effect of DHEA on the activation of endothelial cells induced by a high concentration of glucose. Adhesion on U937 cells, expression of adhesion molecules, production of ROS and NO, expression of eNOS, and translocation of NF-kappa B were evaluated in human umbilical vein endothelial cells (HUVEC) treated with high concentrations of glucose, DHEA, or both. High concentrations of glucose (>20 mM) induced an increase in adhesion, an increment in mainly E-selectin and PECAM-1 expression, as well as in ROS and NO production, eNOS expression, translocation of NF-kappa B, and degradation of its inhibitor I kappa B-alpha. DHEA abolished adhesion and the increase of E-selectin, ICAM-1, VCAM-1, and PECAM-1 induced by glucose. In addition, DHEA completely blocked oxidative stress and decreased translocation of NF-kappa B and the degradation of I kappa B-alpha induced by glucose. These results suggest that DHEA protects against the activation of endothelial cells induced by high concentrations of glucose, indicating that DHEA could be useful in the treatment of hyperglycemia and diabetes. (C) 2011 Elsevier Inc. All rights reserved.
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