Article
Medical Laboratory Technology
Julie A. Ray, Amol O. Bajaj, Erik K. Trier, Lisa M. Johnson
Summary: Cushing syndrome (CS) is caused by an excess of glucocorticoids, leading to symptoms like obesity, moon facies, hirsutism, and reddish-purple stretch marks. Most cases of CS are caused by exogenous glucocorticoids, known as iatrogenic CS. Screening for CS caused by a tumor can be done by measuring cortisol in urine, and detection of prednisone and prednisolone is important in understanding the potential prevalence of iatrogenic CS in patients.
CLINICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Cailing Duan, Yan Wu, Jin Yang, Shenghuo Chen, Yun Pu, Huihua Deng
Summary: The study aimed to develop a sensitive method for simultaneous determination of illicit drugs and steroids in hair to monitor drug exposure and the health status of addicts. The validated method showed good linearity, quantification limits, and recoveries. The method successfully detected analytes in hair of female drug addicts with different drug abuse histories.
Article
Medical Laboratory Technology
Nils Backlund, Goran Brattsand, Staffan Lundstedt, Elisabeth Aardal, Inga Bartuseviciene, Katarina Berinder, Charlotte Hoybye, Pia Burman, Britt Eden Engstrom, Anders Isaksson, Anders Blomgren, Oskar Ragnarsson, Ulrika Ruetschi, Jeanette Wahlberg, Tommy Olsson, Per Dahlqvist
Summary: This study aimed to establish reference intervals for salivary cortisol and cortisone and evaluate their diagnostic accuracy for Cushing's syndrome. The results showed that multiple liquid chromatography-tandem mass spectrometry and immunoassay methods can be used to measure salivary cortisol and cortisone. The diagnostic accuracy for Cushing's syndrome was high, and all methods had high sensitivity and specificity.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Federico Ponzetto, Mirko Parasiliti-Caprino, Fabio Settanni, Antonello Nonnato, Giulio Mengozzi, Ezio Ghigo, Roberta Giordano
Summary: The simultaneous measurement of dexamethasone and cortisol has shown to improve the diagnostic performance of the overnight dexamethasone-suppression test. Additionally, monitoring the levels of corticosteroid drugs can help investigate unexpected changes in steroid hormone levels. This study developed and validated an LC-MS/MS method for quantifying cortisol, cortisone, dexamethasone, and other corticosteroids in serum/plasma samples. The method was successfully applied to patients undergoing the dexamethasone-suppression test and those receiving immunosuppressive treatment after kidney transplant.
Article
Biochemical Research Methods
Loredana Iannella, Fabio Comunita, Francesco Botre, Cristiana Colamonici, Davide Curcio, Xavier de la Torre, Monica Mazzarino
Summary: The urinary excretion profiles of rectally administered glucocorticoids were evaluated in volunteers, showing individual variability influenced by formulation type and absorption area. Prednisone and prednisolone can be detected in urine for at least 30 hours after administration.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Martina Blaschke, Regine Koepp, Frank Streit, Johannes Beismann, Georg Manthey, Mark-Tilmann Seitz, Angelique Kragl, Heide Siggelkow
Summary: The enzyme 11 beta-HSD1 plays a key role in pre-receptor glucocorticoid metabolism, with increased expression leading to adipogenic differentiation in human mesenchymal progenitor cells. Inhibition experiments confirmed the enzyme's specificity for cortisol synthesis and adipogenic differentiation, potentially explaining the link between increased 11 beta-HSD1 expression, higher cortisol levels, and reduced bone quality in old age or in situations of supra-physiological glucocorticoid exposure.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Justin Tapiwa Shenje, Peter Raubenheimer, Lubbe Wiesner, Ian Ross
Summary: Pulmonary tuberculosis is an inflammatory disease characterized by an elevated cortisol/cortisone ratio at the site of infection and changes in cytokine concentrations. Tuberculous pericarditis, a more lethal form, shows a similar inflammatory process in the pericardium. However, the impact of tuberculous pericarditis on pericardial glucocorticoids remains largely unknown.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Veterinary Sciences
Ricardo Videla, Carla Sommardahl, Joe Smith, Deanna M. W. Schaefer, Sherry Cox
Summary: This study investigated the pharmacokinetics of prednisolone in healthy adult alpacas after intravenous and oral administration. The results showed that the serum concentrations of prednisolone were similar to other veterinary species after IV and PO administration. However, oral administration led to changes in blood parameters and bioavailability was determined to be 13.7%. Further research is needed to determine the pharmacodynamics of prednisolone in alpacas.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Review
Clinical Neurology
Itay Lotan, Mark A. Hellmann, Adi Wilf-Yarkoni, Israel Steiner
Summary: Corticosteroids are widely used immunosuppressive agents for conditions like myasthenia gravis, but treatment with them may result in exacerbation of symptoms. The rate of exacerbation varies with different types of corticosteroids, with cortisone having the highest rate and methylprednisolone the lowest. Factors like age, symptom type, disease severity, and thymus-related factors may increase the risk of exacerbation.
JOURNAL OF NEUROLOGY
(2021)
Article
Biochemistry & Molecular Biology
E. Fanjara, G. H. Aas, Y. Cao, V. Kristinova, A. Saeboe (Saebo), A. Stene
Summary: Cortisol and its metabolites play a crucial role in assessing fish stress and welfare status. LC-MS/MS techniques are effective in identifying and measuring these compounds. The compound 5 beta-THE is proposed as a potential stress biomarker. Pooling samples from multiple individuals is a reliable approach for stress evaluation in farmed fish.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Environmental Sciences
Honglong Zhang, Jun Yan, Guole Nie, Danna Xie, Bin Luo, Jingping Niu, Haiping Wang, Xun Li
Summary: This study found that exposure to cadmium or lead alone leads to a decrease in serum cortisol and cortisone levels, and co-exposure to cadmium and lead further reduces these hormone levels. It was also found that cadmium completely mediates the relationship between lead and the hormones, and lead may weaken the negative relationship between cadmium and the hormones. These results indicate that exposure to cadmium and lead, either alone or in combination, interferes with the homeostasis of serum cortisol and cortisone levels.
Article
Food Science & Technology
Yi Yang, Jiachen Shi, Jie Yin, Yunjia Yang, Bing Shao, Jing Zhang
Summary: This study investigated the presence of glucocorticoids in aquatic food, revealing significantly higher levels in freshwater fish compared to marine fish. The research also suggested a correlation between GCs concentrations and different killing methods, as well as a positive relationship with the percentage of freshwater fish consumption.
Article
Endocrinology & Metabolism
Basil George Issa, Fahmy W. F. Hanna, Anthony A. Fryer, Grace Ensah, Ikenna Ebere, David Marshall, Brian Keevil
Summary: A strong correlation was found between salivary cortisone and serum cortisol in patients with adrenal incidentalomas, suggesting that salivary cortisone can be used as an alternative sampling method for measuring serum cortisol.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Review
Biochemistry & Molecular Biology
Maria Botia, Damian Escribano, Silvia Martinez-Subiela, Asta Tvarijonaviciute, Fernando Tecles, Marina Lopez-Arjona, Jose J. Ceron
Summary: The main glucocorticoids involved in the stress response, such as cortisol, cortisone, and corticosterone, are widely used as biomarkers for evaluating physiological response to stress. Measurement of total glucocorticoids, which encompasses various types of glucocorticoids, is commonly performed using immunoassays. This review explores the characteristics of these glucocorticoids, techniques for their quantification, and the importance of measuring multiple types of glucocorticoids in assessing welfare.
Article
Endocrinology & Metabolism
Joseph J. Tonge, Brian G. Keevil, Jessica N. Craig, Martin J. Whitaker, Richard J. Ross, Charlotte J. Elder
Summary: The study compared different methods of salivary collection and found that Salivette, passive drool, and SalivaBio all yielded comparable concentrations of salivary cortisol and cortisone, which were stable under conditions replicating home collection. The SaliPac device was found to be an acceptable option for salivary sampling in young children.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Chemistry, Applied
F. Arioli, E. Pasquale, S. Panseri, L. Bonizzi, G. F. Labella, A. Casati, S. Foschini, L. Chiesa
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2015)
Article
Chemistry, Applied
Luca Chiesa, Elisa Pasquale, Sara Panseri, Francesca T. Cannizzo, Bartolomeo Biolatti, Radmila Pavlovic, Francesco Arioli
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2015)
Article
Environmental Sciences
L. M. Chiesa, G. F. Labella, A. Giorgi, S. Panseri, R. Pavlovic, S. Bonacci, F. Arioli
Article
Environmental Sciences
L. M. Chiesa, G. F. Labella, S. Panseri, R. Pavlovic, S. Bonacci, F. Arioli
Article
Biochemical Research Methods
Luca Maria Chiesa, Giuseppe Federico Labella, Elisa Pasquale, Sara Panseri, Radmila Pavlovic, Francesco Arioli
Article
Food Science & Technology
Luca Maria Chiesa, Maria Nobile, Bartolomeo Biolatti, Radmila Pavlovic, Sara Panseri, Francesca Tiziana Cannizzo, Francesco Arioli
Article
Agriculture, Multidisciplinary
Luca Maria Chiesa, Maria Nobile, Sara Panseri, Bartolomeo Biolatti, Francesca Tiziana Cannizzo, Radmila Pavlovic, Francesco Arioli
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2016)
Article
Agriculture, Dairy & Animal Science
L. Chiesa, S. Pavone, E. Pasquale, R. Pavlovic, S. Panseri, A. Valiani, F. Arioli, E. Manuali
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2017)
Article
Chemistry, Applied
Luca Maria Chiesa, Maria Nobile, Sara Panseri, Francesco Arioli
Article
Food Science & Technology
Sara Panseri, Elisabetta Bonerba, Maria Nobile, Federica Di Cesare, Giacomo Mosconi, Francisco Cecati, Francesco Arioli, Giuseppina Tantillo, Luca Chiesa
Article
Food Science & Technology
Luca Maria Chiesa, Federica Di Cesare, Maria Nobile, Roberto Villa, Lucia Decastelli, Francesca Martucci, Mauro Fontana, Radmila Pavlovic, Francesco Arioli, Sara Panseri
Summary: This study aimed to verify the presence of antibiotic residues in milk after therapeutic treatments, showing that 29% of samples had antimicrobial drugs and their metabolites, with 9% of samples also revealing undisclosed treatments. Furthermore, two new enrofloxacin metabolites were proposed, highlighting the potential risk of metabolites to consumers and the processing of milk in the cheese industry.
Article
Biochemical Research Methods
Francesco Arioli, Maria Cristina Gamberini, Radmila Pavlovic, Federica Di Cesare, Susanna Draghi, Giulia Bussei, Francesca Mungiguerra, Alessio Casati, Marco Fidani
Summary: The objective of this research was to develop a LC-MSn method for the determination of free cortisol and its metabolites in human urine. The method was validated for accuracy, precision, linearity, and recovery. It was successfully applied to urine samples from healthy subjects and patients with cortisol-related diseases. Furthermore, the method has potential applications in antidoping control and the study of prednisolone metabolism.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Giacomo Mosconi, Federica Di Cesare, Francesco Arioli, Maria Nobile, Doriana E. A. Tedesco, Luca M. Chiesa, Sara Panseri
Summary: This study analyzed the presence of POPs in commonly consumed fish species in Italy and found that the concentrations of these pollutants did not exceed maximum limits or safe values, indicating low health risks for consumers.
Article
Food Science & Technology
Maria Nobile, Luigi Danesi, Radmila Pavlovic, Giacomo Mosconi, Federica Di Cesare, Francesco Arioli, Roberto Villa, Luca Maria Chiesa, Sara Panseri
Summary: Chlorates and perchlorates are endocrine disruptors and emerging environmental contaminants found in various foods. This study assessed the presence of these substances in raw bovine milk samples from Lombardy, Italy. The results showed that most samples contained chlorates and perchlorates, but there was no risk for Italian consumers.
Article
Food Science & Technology
Maria Nobile, Giacomo Mosconi, Luca Maria Chiesa, Sara Panseri, Luigi Danesi, Ermelinda Falletta, Francesco Arioli
Summary: Fish is important in an Italian diet, but can accumulate contaminants such as PFASs and PTEs. This study investigated the presence of these contaminants in salted and canned anchovies collected from different fishing areas in Italy over 10 months. The results showed that the risk for consumers was generally low, except for one sample with concerns about nickel toxicity.
Article
Biochemistry & Molecular Biology
Olexander M. Semenenko, Victoria V. Lipson, Alina O. Sadchenko, Olga V. Vashchenko, Natalia A. Kasian, Liliia Sviechnikova, Longin M. Lisetski, Mykola L. Babak, Volodymyr M. Vakula, Oleksandr Borysov, Yuliia Holota, Sergey O. Zozulya, Petro O. Borysko, Olexander Mazepa
Summary: An efficient protocol for the synthesis of novel methotrexate-betulonic acid hybrids has been developed and their structures and properties have been fully established. The hybrids showed inferior interactions with artificial lipid membranes, possibly due to their increasing lipophilicity.
Article
Biochemistry & Molecular Biology
Mohadese Yaghoobi Anzabi, Piotr Cmoch, Roman Luboradzki, Zbigniew Pakulski
Summary: Friedelin and 3-acetoxyfriedel-3-en-2-one, known as friedelane triterpenoids, have been isolated from cork smoker wash solids and used as starting materials for the synthesis of new friedelane derivatives. Reduction reactions result in the formation of friedelinol and epi-cerin derivatives.
Article
Biochemistry & Molecular Biology
Detlef Thieme, Aniko Krumbholz, Martin Bidlingmaier, Christoph Geffert, Annika Hameder, Andreas Stoever, Matthias Graw, Annekathrin M. Keiler
Summary: Ethanol can affect testosterone metabolism and potentially influence steroid biosynthesis and blood steroid profiles. The study emphasizes the importance of sufficient time interval from food and ethanol intake during blood sampling, particularly for diagnostics in doping control.
Article
Biochemistry & Molecular Biology
Masanobu Murao, Tetsuo Imano, Yoshinobu Sato, Masaaki Nakajima
Article
Biochemistry & Molecular Biology
Jingyuan Wang, Ye Feng, Brian Liu, Wen Xie
Summary: Sulfation and desulfation of steroids are opposing processes that regulate steroid homeostasis and are involved in steroid-related diseases. Cytosolic sulfotransferase and steroid sulfatase modify and regulate steroids to affect hormonal dysregulation, metabolic diseases, and cancer.
Article
Biochemistry & Molecular Biology
Noah K. Babel, Brian J. Feldman
Summary: Our research identified a previously unrecognized ADAMTS1-PTN-Wnt pathway in regulating adipose precursor cell behavior. The non-muscle myosin protein MYH9 was found to be a key target of this pathway for modulating adipogenesis. These findings offer potential for developing novel therapeutics for obesity and related metabolic disorders.
Article
Biochemistry & Molecular Biology
Martiniano Bello
Summary: This study explores the structural and thermodynamic basis of molecular recognition between ERβ and DPN and its derivatives using molecular dynamics simulations and end-point methods. The results show that the MMGBSA approach is able to reproduce experimental trends and identify eight residues that strongly interact with the ligands.