Article
Biotechnology & Applied Microbiology
Manon Jammes, Romain Contentin, Fabrice Audigie, Frederic Casse, Philippe Galera
Summary: This study tested the effects of different storage conditions on the secretome of mesenchymal stromal cells (MSCs) and investigated the enhancement of MSC secretome properties through cytokine priming. The results showed that secretome stored at -80°C, -20°C, and 4°C improved collagen protein accumulation, cell proliferation, and inflammation reduction in chondrocytes. Cytokine priming had different effects on the expression of immunomodulatory molecules and influenced the behavior of equine articular chondrocytes (eACs). These findings demonstrate the potential of MSC-conditioned media for improving the management of equine osteoarthritis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biology
Seung Yeon Lee, Jin Woo Lee
Summary: This article reviews the recent advances in the application of 3D culture techniques for cartilage regeneration, focusing on the use of 3D spheroid culture system using stem cells. Exosomes, as intercellular communication systems, play a role in promoting chondrogenic differentiation and proliferation for cartilage repair. Further mechanistic studies are needed to enhance the therapeutic options for osteoarthritis using MSC-derived exosomes.
Article
Orthopedics
Jasmijn Korpershoek, Margot Rikkers, Fleur S. A. Wallis, Koen Dijkstra, Marije te Raa, Peter de Knijff, Daniel B. F. Saris, Lucienne A. Vonk
Summary: This study found that there is transport of mitochondria between mesenchymal stromal cells (MSCs) and chondrocytes, which may contribute to the mechanism of action of MSCs on chondrocytes. However, no transferred mitochondria could be traced back in vivo.
Article
Biotechnology & Applied Microbiology
Erwin Pavel Lamparelli, Maria Camilla Ciardulli, Valentina Giudice, Pasqualina Scala, Rosa Vitolo, Tina Patricia Dale, Carmine Selleri, Nicholas Robert Forsyth, Nicola Maffulli, Giovanna Della Porta
Summary: This study investigated the chondrogenic potentials of 3D high-density cultures of bone marrow and Wharton's Jelly-derived mesenchymal stromal cells (MSCs) supplemented with hTGF-beta 1. The study found that high-density cultures promoted chondrogenesis and that Wharton's Jelly-derived MSCs showed potential for chondrogenic commitment. These findings have important implications for cartilage regeneration and tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Cell & Tissue Engineering
Xiao-Na Xiang, Si-Yi Zhu, Hong-Chen He, Xi Yu, Yang Xu, Cheng-Qi He
Summary: Osteoarthritis is a common degenerative disease that causes high socioeconomic costs and disability rates. The knee joint is most commonly affected, characterized by progressive destruction of articular cartilage, loss of extracellular matrix, and inflammation. MSC-based therapy has been explored as a new regenerative treatment for knee osteoarthritis. However, the detailed functions and mechanisms of MSC-based therapy, especially for cartilage regeneration, have not been explained.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Giuliana Biagini, Alexandra Cristina Senegaglia, Tarciso Pereira, Lucas Freitas Berti, Bruna Hilzendeger Marcon, Marco Augusto Stimamiglio
Summary: Tissue engineering involves the combination of biomaterials, cells, and bioactive molecules to regenerate tissues. This study found that on scaffolds with different pore sizes, adipose-derived stromal cells showed enhanced cell cycle progression, while expanded CD133(+) cells experienced arrest in the G1 phase and reduced proliferation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Wenguang Liu, Alexander S. Brodsky, Meng Feng, Yajun Liu, Jing Ding, Chathuraka T. Jayasuriya, Qian Chen
Summary: Aging OA-MSC in human osteoarthritic cartilage can lead to cell senescence, fibrosis, and inflammation, expressing a pro-inflammatory senescence associated secretory phenotype that may contribute to OA pathogenesis. OAC acts as the main recipient of inflammatory signals by expressing receptors of cytokines. Through replication-driven OAC dedifferentiation and MSC senescence, OA-MSC becomes an internal source of sterile inflammation in human cartilage joint.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Yichun Wang, Hyunsu Jeon
Summary: 3D cell cultures play a crucial role in drug discovery and development, but face challenges due to their complexity, time-consuming process, and lack of compatibility with traditional screening protocols. The integration of micro fabrication techniques, automation systems, and high-throughput analytical tools is needed to reveal the effects of therapeutics in 3D cultures.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2022)
Review
Cell Biology
Ekaterina Sergeevna Novoseletskaya, Pavel Vladimirovich Evdokimov, Anastasia Yurievna Efimenko
Summary: The extracellular matrix plays a crucial role in regulating cell behavior and fate in stem cell microenvironments. Different types of stem cells possess molecules that interact with the extracellular matrix and cause changes in gene expression. Signals from the extracellular matrix are important for embryonic and postnatal development, as well as tissue renewal and repair. Various methods are used to study the cellular signaling networks from the extracellular matrix and predict the pathways in target cells.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Cell Biology
Jaroslav Fabik, Viktorie Psutkova, Ondrej Machon
Summary: This study presents a model for studying hyoid apparatus development and finds that the lack of Meis2 in mice leads to abnormal development of the hyoid apparatus. The loss of Meis2 results in abnormal mesenchymal condensations, ectopic cartilage and bone formation, and increased proliferation of cartilage precursors. The study also shows that the reduced expression of PBX1 and HAND2 is associated with the loss of Meis2. These findings are important for understanding the developmental mechanisms of the hyoid apparatus.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Haoli Ying, Chengchun Shen, Ruolang Pan, Xiongfeng Li, Ye Chen
Summary: Osteoarthritis (OA) is a degenerative joint disease that commonly affects older adults and those who have undergone meniscal surgery, causing significant suffering worldwide. Retrograde changes in the articular cartilage are a major pathological feature of OA. Mesenchymal stromal cells (MSCs) have the potential to differentiate into chondrocytes and promote cartilage regeneration, making them promising for OA treatment. However, optimizing the therapeutic effect of MSCs in the joint cavity remains a challenge. Hydrogels made of different biomaterials have emerged as an ideal carrier for MSCs, and this review focuses on the influence of hydrogel mechanical properties on MSC efficacy in OA treatment, comparing them with articular cartilage and providing insights for developing modified hydrogels to enhance MSC therapeutic effect.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Marie Maumus, Guillaume Fonteneau, Maxime Ruiz, Said Assou, Hassan Boukhaddaoui, Philippe Pastoureau, Frederic De Ceuninck, Christian Jorgensen, Daniele Noel
Summary: NMB is a newly identified chondroinductive bioactive factor that upregulates the key chondrogenic transcription factor Sox9 through modulation of the Ca2+ signaling pathway and calcineurin activity. NMB plays a significant role in chondrogenesis.
CELL AND BIOSCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Luisa Milazzo, Francesca Vulcano, Giampiero Macioce, Giovanna Marziali, Francesca Iosi, Lucia Bertuccini, Mario Falchi, Francesco Rech, Adele Giampaolo, Raffaella Pecci, Ilaria Campioni, Rossella Bedini
Summary: This study aimed to investigate the feasibility of silk fibroin porous scaffolds in supporting the adhesion of human Wharton's jelly mesenchymal stem cells, with findings from micro-CT, confocal analysis, and SEM showing the high suitability of the designed SF scaffolds.
APPLIED SCIENCES-BASEL
(2021)
Review
Cell Biology
Cristina Manferdini, Elena Gabusi, Yasmin Saleh, Enrico Lenzi, Giovanni D'Atri, Leonardo Ricotti, Gina Lisignoli
Summary: This systematic review focuses on the characteristics of hydrogels used for embedding MSCs in in vitro/ex vivo studies, in vivo OA models, and clinical trials for cartilage regeneration in OA. The review identifies that hydrogels have shown promising results in these studies and trials, providing positive impact on patients with OA.
Article
Chemistry, Multidisciplinary
Tian-Ji Ji, Bei Feng, Jie Shen, Min Zhang, Yu-Qing Hu, Ai-Xia Jiang, Di-Qi Zhu, Yi-Wei Chen, Wei Ji, Zhen Zhang, Hao Zhang, Fen Li
Summary: In this study, axitinib-loaded nanofibrous membranes effectively inhibited ossification tendency and stabilized the cartilaginous phenotype of MSCs-engineered cartilage in the subcutaneous environment, demonstrating a novel strategy for stable chondrogenesis.
Article
Orthopedics
Denis Baranovskii, Jan Demner, Sylvia Nuernberger, Alexey Lyundup, Heinz Redl, Morgane Hilpert, Sebastien Pigeot, Michael Krasheninnikov, Olga Krasilnikova, Ilya Klabukov, Vladimir Parshin, Ivan Martin, Didier Lardinois, Andrea Barbero
Summary: In this study, the researchers investigated the use of decellularized laser-perforated tracheal cartilage (LPTC) as a substrate for colonization by adult nasal chondrocytes (NCs) to repair tracheal defects. The results showed that NCs could efficiently colonize the LPTCs and produce new cartilage tissue suitable for tracheal repair.
Review
Biotechnology & Applied Microbiology
Andrea Mainardi, Elena Cambria, Paola Occhetta, Ivan Martin, Andrea Barbero, Stefan Schaeren, Arne Mehrkens, Olga Krupkova
Summary: Discogenic back pain is a common pathology that affects the quality of life for millions of people. Current treatments focus on symptom reduction rather than targeting the underlying degenerative processes. Existing disease models do not fully replicate the course of degenerative disc disease. Organs-on-chip (OoC) platforms are microfluidic devices that aim to replicate tissue functions and investigate pathological alterations. They have the potential to mimic physiological or aberrant mechanical loading in intervertebral discs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jesil Kasamkattil, Anna Gryadunova, Ivan Martin, Andrea Barbero, Stefan Schaeren, Olga Krupkova, Arne Mehrkens
Summary: Degenerative disc disease is a painful condition that often leads to disability. Spheroid-based approaches show promise in overcoming challenges associated with cell-based intervertebral disc therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Cell & Tissue Engineering
Daniel J. Weiss, Anthony Filiano, Jacques Galipeau, Maroun Khoury, Mauro Krampera, Manoj Lalu, Katarina Le Blanc, Jan Nolta, Donald G. Phinney, Patricia R. M. Rocco, Yufang Shi, Karin Tarte, Sowmya Viswanathan, Ivan Martin
Article
Engineering, Biomedical
Loraine Kouba, Joel Burgin, Gordian Born, Giuseppe Perale, Dirk J. Schaefer, Arnaud Scherberich, Sebastien Pigeot, Ivan Martin
Summary: We developed a composite material consisting of an osteoconductive material and a tissue engineered human cartilage extracellular matrix (ECM) with strong osteoinductive properties. The low amount of osteoinductive ECM enhanced host cell recruitment and promoted the formation of large vascularized bone structures in two different animal models, including a challenging prefabricated bone-flap model. This study demonstrates the potential of this off-the-shelf human material for accelerated healing in clinical applications.
ACTA BIOMATERIALIA
(2022)
Article
Biochemical Research Methods
Gordian Born, Evelia Plantier, Guido Nannini, Alessandro Caimi, Andrea Mazzoleni, M. Adelaide Asnaghi, Manuele G. Muraro, Arnaud Scherberich, Ivan Martin, Andres Garcia-Garcia
Summary: Bioreactors that allow direct perfusion of cell suspensions or culture media through 3D scaffolds are widely used in tissue engineering to improve cell seeding efficiency, uniformity of cell distribution, and tissue development. A macro-scale U-shaped bioreactor has been previously developed, but the perfusion flow in the peripheral regions was not uniform. In this study, the design of the perfusion chamber has been optimized to provide more homogeneous perfusion flow through the scaffold. Furthermore, a miniaturized version of the flow-optimized bioreactor has been proposed for medium-throughput assays.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Engineering, Biomedical
Yawei Gu, Sebastien Pigeot, Lucas Ahrens, Fabian Tribukait-Riemenschneider, Melika Sarem, Francine Wolf, Andres Garcia-Garcia, Andrea Barbero, Ivan Martin, V. Prasad Shastri
Summary: By developing a bioink system with appropriate rheological properties and using 3D bioprinting technology, it is possible to create high cell density structures of predefined geometry, which can then be implanted into nude mice to generate bone tissue with neovascularization. This method demonstrates the potential for fabricating complex bone tissue for clinical applications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Cell Biology
Shikha Chawla, Andrea Mainardi, Nilotpal Majumder, Laura Doenges, Bhupendra Kumar, Paola Occhetta, Ivan Martin, Christian Egloff, Sourabh Ghosh, Amitabha Bandyopadhyay, Andrea Barbero
Summary: Articular cartilage has limited self-healing ability, which can lead to osteoarthritis. During the progression of osteoarthritis, there are significant changes in gene expression and phenotype of articular chondrocytes, which play a crucial role in the pathogenesis of the disease. Current treatment strategies mainly focus on cartilage inflammation and degradation, and there is still a lack of knowledge about the mechanisms behind the hypertrophic chondrocyte phenotype.
Article
Cell Biology
Boris Dasen, Sebastien Pigeot, Gordian Manfred Born, Sophie Verrier, Olga Rivero, Petra S. Dittrich, Ivan Martin, Maria Filippova
Summary: This study found that pericytes express not only the classical N-cadherin, but also the atypical T-cadherin. T-cadherin plays an important role in regulating the proliferation, migration, invasion, and interactions of pericytes with endothelial cells.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Cell & Tissue Engineering
Sowmya Viswanathan, Katarina Le Blanc, Rachele Ciccocioppo, Georges Dagher, Anthony J. Filiano, Jacques Galipeau, Mauro Krampera, Lena Krieger, Manoj M. Lalu, Jan Nolta, Viviana Marcela Rodriguez Pardo, Yufang Shi, Karin Tarte, Daniel J. Weiss, Ivan Martin
Summary: The International Standards Organization (ISO) has recently published ISO standardization documents for MSC(WJ) and MSC(M) biobanking, which standardize the terminology and functional characterization of MSCs. These documents, developed with input from ISCT, provide requirements and recommendations for functional characterization of MSC(WJ) and MSC(M) and represent an international consensus on MSC identity and characterization. They are an important first step in standardizing MSC biobanking and characterization for research and development.
Article
Cell & Tissue Engineering
Anke Wixmerten, Sylvie Miot, Patrick Bittorf, Francine Wolf, Sandra Feliciano, Stephan Hackenberg, Sebastian Hasner, Werner Krenger, Martin Haug, Ivan Martin, Oliver Pullig, Andrea Barbero
Summary: This article describes the regulatory compliant change of a raw material in the development of a medicinal product. The substitution of autologous serum with clinical-grade human platelet lysate (hPL) in the manufacturing process was shown to enhance cell proliferation and gene expression without significant changes in product quality. The modified process was accepted by national competent authorities and applied in ongoing clinical tests of N-TEC.
Article
Biotechnology & Applied Microbiology
Jesil Kasamkattil, Anna Gryadunova, Raphael Schmid, Max Hans Peter Gay-Dujak, Boris Dasen, Morgane Hilpert, Karoliina Pelttari, Ivan Martin, Stefan Scharen, Andrea Barbero, Olga Krupkova, Arne Mehrkens
Summary: An advanced 3D microtissues model containing human degenerating nucleus pulposus tissue was developed to mimic the microenvironment of early-stage degenerative disc disease. Nasal chondrocytes in spheroidal organization showed superior regenerative performance compared to cell suspension, and pre-conditioning with IL-1Ra further improved their ability to counteract inflammation and support new matrix production. This study is of great importance for therapeutic strategies in degenerative disc disease.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Andres Garcia-Garcia, Sebastien Pigeot, Ivan Martin
Summary: The contribution of the immune system to tissue regeneration is increasingly recognized, suggesting that tissue engineering strategies and biomaterials design should focus on regulating early immunological processes. This study aimed to enhance the osteoinductivity of engineered extracellular matrices (ECMs) by enriching their repertoire of immunomodulatory factors. The results showed that priming with IL1 beta and overexpressing BMP-2 enabled the engineering of ECMs with preserved osteoinductive signals and increased levels of angiogenic and pro-inflammatory molecules. These IL1 beta-induced ECMs enhanced the inflammatory phase and ultimately led to faster and more efficient bone formation.
BIOACTIVE MATERIALS
(2023)
