Article
Cell & Tissue Engineering
Changzhen Wang, Hongmei Ning, Jiao Gao, Teng Xue, Ming Zhao, Xiaoxia Jiang, Xiaoming Zhu, Ximin Guo, Hong Li, Xiaoyan Wang
Summary: This study investigated the impact of hematopoietic cells on MSCs and found that partial deletion of the Pten gene in hematopoietic cells can lead to abnormal differentiation of MSCs. There is a reciprocal relationship between hematopoietic cells and MSCs, with an important role in bone development.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Multidisciplinary Sciences
Akansha M. Shah, Lei Guo, Maria Gabriela Morales, Priscilla Jaichander, Kenian Chen, Huocong Huang, Karla Cano Hernandez, Lin Xu, Rhonda Bassel-Duby, Eric N. Olson, Ning Liu
Summary: Rhabdomyosarcoma (RMS) is a common soft tissue sarcoma in children that fails to differentiate despite expression of the myogenic determination protein MYOD. Overexpression of the TWIST2 transcription factor is frequently seen in fusion-negative RMS (FN-RMS). This study shows that knockdown of TWIST2 allows FN-RMS cells to undergo terminal myogenesis and reduces tumor growth. Mechanistically, TWIST2 controls H3K27 acetylation at distal enhancers by interacting with chromatin remodelers to regulate target genes involved in growth and myogenesis. Targeting TWIST2-regulated pathways may provide a potential treatment strategy for FN-RMS.
Article
Biochemistry & Molecular Biology
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: In this study, TNS3 was found to be involved in the fate decision of human bone marrow-derived stromal cells (BMSCs), regulating osteogenesis and adipogenesis through the control of RhoA activity and cytoskeletal reorganization. The findings highlight the crucial role of TNS3 in bone metabolism.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Medicine, Research & Experimental
Leiluo Yang, Qing Li, Junhong Zhang, Pengcheng Li, Pingjiang An, Chunqing Wang, Pingsheng Hu, Xue Zou, Xiaowei Dou, Lixin Zhu
Summary: The study investigated the role of Wnt7a in bone formation and demonstrated its key role in MSC differentiation into osteoblasts, offering a potential therapeutic target for bone-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Cell & Tissue Engineering
Naruphong Phunikom, Nittaya Boonmuen, Pakpoom Kheolamai, Kanoknetr Suksen, Sirikul Manochantr, Chairat Tantrawatpan, Duangrat Tantikanlayaporn
Summary: Andrographolide (AP) has been shown to promote proliferation, osteogenic differentiation, and bone formation of placenta-derived mesenchymal stem cells. It may be an effective novel agent for improving placenta-derived MSCs and stem cell-based therapy for bone regeneration. The compound enhances Wnt/beta-catenin signaling and osteoblast-specific gene expressions, promoting bone formation and increasing osteocalcin levels.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell Biology
Xiaoyang Liu, Zongke Zhou, Wei-Nan Zeng, Qin Zeng, Xingdong Zhang
Summary: Osteoimmunology involves crosstalk between the skeletal and immune systems. Toll-like receptors (TLRs) play a crucial role in bone remodeling by directly impacting MSC osteogenic differentiation. Understanding TLRs' role has significant implications for advancing bone regeneration and repair strategies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Jong Bo Park, Ji Yeon Ahn, Woo Sub Yang, Seong Chae Park, Young Jin Jung, Myungook Lee, Byung Hee Hong, Jeong Mook Lim
Summary: This study investigated the effect of nanoscale spacing by stacked graphene film on osteogenic differentiation of human adipose-derived mesenchymal stromal cells. The results showed that osteogenic differentiation was promoted as the stacking layer increased, with the most significant effects observed in the three layers. The study demonstrated that multilayer-stacked graphene created nanoscale spaces promote calcium deposit and cytoskeletal integrity in hADSC-related, in vitro-osteogenesis.
Article
Veterinary Sciences
Antonella Liza Pantaleoni Andrietti, Sushmitha S. Durgam, Brittany Naumann, Matthew Stewart
Summary: This study compared osteogenesis in equine bone marrow-, synovium- and adipose-derived cells and found that bone marrow-derived cells had higher basal expression of Osterix, indicating their higher osteogenic potential. In osteogenic medium, these cells quickly developed dense multicellular aggregates with strong mineralization and alkaline phosphatase activity. On the other hand, synovial and adipose cell cultures showed less matrix mineralization.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Cell Biology
Marta S. Carvalho, Laura Alves, Isabel Bogalho, Joaquim M. S. Cabral, Claudia L. da Silva
Summary: The study found that aging impairs the proliferative and osteogenic capacities of MSC, but exposure to ECM derived from young cells can help rejuvenate aged MSC. The ECM plays a crucial role in the quantity and quality of MSC, especially in terms of osteogenic differentiation capacity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Victor Gustavo Balera Brito, Mariana Sousa Patrocinio, Ayna Emanuelli Alves Barreto, Sabrina Cruz Tfaile Frasnelli, Vanessa Soares Lara, Carlos Ferreira Santos, Sandra Helena Penha Oliveira
Summary: The study demonstrated that Telmisartan (TELM) inhibited osteogenic differentiation and mineralization of SHR BMSC, favoring an adipogenic phenotype due to Pparg upregulation.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hyo-Eun Son, Won-Gu Jang
Summary: This study reveals that Cip2A plays a critical role in regulating osteoblast differentiation by inducing ERK phosphorylation in MG63 cells.
Article
Biotechnology & Applied Microbiology
Thora Bjorg Sigmarsdottir, Sarah McGarrity, Adrian Lopez Garcia de Lomana, Aristotelis Kotronoulas, Snaevar Sigurdsson, James T. Yurkovich, Ottar Rolfsson, Olafur Eysteinn Sigurjonsson
Summary: This study aimed to characterize the metabolic shifts that occur during osteogenesis by profiling extracellular metabolites and classifying the process into three metabolic stages. The identified metabolic stages provide a framework for further investigations to identify biomarkers of osteogenic differentiation and optimize MSC differentiation for clinical applications.
BIOENGINEERING-BASEL
(2021)
Article
Cell Biology
Jiachen Lin, Zhifa Zheng, Jieying Liu, Guihua Yang, Ling Leng, Hai Wang, Guixing Qiu, Zhihong Wu
Summary: Nutritional microenvironment plays a crucial role in determining the specification of progenitor cells, with lipid availability impacting osteogenesis in skeletal progenitors. The study found that lipid scarcity suppresses the osteogenic differentiation of BMSCs, while LRP5 mediates lipid uptake in BMSCs and influences osteogenic specification.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Wenli Gu, Xiao Jiang, Wei Wang, Prabhakar Mujagond, Jingpeng Liu, Zhaoyi Mai, Hai Tang, Simin li, Hui Xiao, Jianjiang Zhao
Summary: LINC01485 was up-regulated during osteogenic differentiation of hBMSCs and promoted osteogenesis by acting as a ceRNA of miR-619-5p to influence RUNX2 expression. miR-619-5p inhibited osteogenic differentiation. The LINC01485/miR-619-5p/RUNX2 axis could serve as a potential target in bone tissue engineering.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Thorben Sauer, Giulia Facchinetti, Michael Kohl, Justyna M. Kowal, Svitlana Rozanova, Julia Horn, Hagen Schmal, Ivo Kwee, Arndt-Peter Schulz, Sonja Hartwig, Moustapha Kassem, Jens K. Habermann, Timo Gemoll
Summary: This study characterized the differences between MSCs and cells after adipogenic or osteoblastic differentiation at the proteome level. Specific proteins related to bone formation were identified, which could be used for regenerative medicine in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Christine M. Khella, Rojiar Asgarian, Judith M. Horvath, Bernd Rolauffs, Melanie L. Hart
Summary: This review aims to explore the disease process of post-traumatic osteoarthritis (PTOA) of the knee joint, analyzing clinical data on inflammatory biomarkers and identifying key factors leading to PTOA. The study also discusses methods for simulating knee trauma and inflammation, comparing pro-inflammatory cytokine concentrations in models with those in synovial fluid after knee trauma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Jasmin C. Lauer, Mischa Selig, Melanie L. Hart, Bodo Kurz, Bernd Rolauffs
Summary: Various stimuli and signaling pathways can modulate the chondrocyte phenotype, impacting their function. Manipulating the cytoskeleton and signaling processes offers a potential avenue for controlling and repairing chondrocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Walter Linzenbold, Andreas Fech, Manuela Hofmann, Wilhelm K. Aicher, Markus D. Enderle
Summary: The traditional method of injecting cells with a needle through a cystoscope in pig urethral sphincter muscle has a high failure rate, so a new needle-free water-jet system for precise injection of cells was developed and tested. This system is designed to fit in endoscopes and allows for minimally invasive applications, with settings that enable high cell viability post-injection in different tissue layers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Bastian Amend, Niklas Harland, Jasmin Knoll, Arnulf Stenzl, Wilhelm K. Aicher
Summary: SUI is a significant health concern impacting patients' quality of life severely. Different animal models are developed to investigate SUI mechanisms, providing insights into pathomechanisms, therapeutic efficacy, and mimicking SUI risk factors. Large animal models such as pig, goat, dog provide advantages for studying surgical regimens or cell therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Hui Che, Mischa Selig, Bernd Rolauffs
Summary: This review explores the potential therapeutic applications and successful control of various cell functions through micro-patterning. It provides comprehensive summaries of established micro-pattern designs and fabrication technologies, and suggests a quality evaluation score.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Engineering, Biomedical
Mathaeus Tschaikowsky, Sofia Brander, Vanessa Barth, Ralf Thomann, Bernd Rolauffs, Bizan N. Balzer, Thorsten Hugel
Summary: This study reveals changes in the thickness and composition of collagen fibers at the onset of osteoarthritis. Fibrocartilage-like tissue rich in type I collagen is formed in intact cartilage during the early stages of the disease, while thick fibers are completely absent in advanced osteoarthritis. The findings provide insights into the understanding of other native biological tissues and the development of sustainable biomaterials.
ACTA BIOMATERIALIA
(2022)
Article
Biochemistry & Molecular Biology
Ruizhi Geng, Niklas Harland, Ivonne A. Montes-Mojarro, Falko Fend, Wilhelm K. Aicher, Arnulf Stenzl, Bastian Amend
Summary: In this study, it was found through experiments that the expression of CD24 in bladder cancer cell organoids may serve as an indicator for the prolonged proliferation potential of the cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Holger Jahr, Anna E. van der Windt, Ufuk Tan Timur, Esther B. Baart, Wei-Shiung Lian, Bernd Rolauffs, Feng-Sheng Wang, Thomas Pufe
Summary: Increasing osmolarity to physiological levels promotes chondrogenic differentiation and marker expression in chondroprogenitor cells. The calcineurin inhibitor FK506 enhances this effect by modulating TGF-beta signaling at the type I receptor. Our findings provide insights into the mechanisms underlying cartilage repair strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Niklas Harland, Florian B. Maurer, Tanja Abruzzese, Cornelia Bock, Ivonne A. Montes-Mojarro, Falko Fend, Wilhelm K. Aicher, Arnulf Stenzl, Bastian Amend
Summary: The elevated expression of CD276 on tumor cell surfaces is associated with tumor cell proliferation and prognosis. CD276 mRNA and protein expression are negatively correlated with tumor cell proliferation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Jules A. Menima-Medzogo, Kathrin Walz, Jasmin C. Lauer, Gopakumar Sivasankarapillai, F. Robert Gleuwitz, Bernd Rolauffs, Marie-Pierre Laborie, Melanie L. Hart
Summary: As global efforts to use eco-friendly and reusable materials increase, the use of lignin from waste biomass is gaining interest in biomedical research. In this study, we found that fractionated low MW beechwood-derived organosolv lignin is non-cytotoxic to human cell types commonly used in tissue engineering. Furthermore, we demonstrated that organosolv lignin can be used to fabricate cell scaffolds and enhance their stiffness and cell attachment. This suggests that organosolv lignin may have potential applications in tissue engineering.
Article
Biochemistry & Molecular Biology
Yi Wei, Bastian Amend, Tilman Todenhoefer, Nizar Lipke, Wilhelm K. Aicher, Falko Fend, Arnulf Stenzl, Niklas Harland
Summary: This article introduces the generation of organoids from urinary tract tumor samples and investigates the drug response of these organoids. The results show that upper tract urothelial carcinoma cells and bladder cancer cells in organoids display different sensitivities to different drugs compared to two-dimensional cultures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lucas Becker, Felix Fischer, Julia L. Fleck, Niklas Harland, Alois Herkommer, Arnulf Stenzl, Wilhelm K. Aicher, Katja Schenke-Layland, Julia Marzi
Summary: This study established label-free imaging procedures, including Raman microspectroscopy (RMS) and fluorescence lifetime imaging microscopy (FLIM), for non-invasive and molecular-sensitive monitoring of tumor-drug interactions in bladder cancer organoid models. The effects of drugs on subcellular structures and cell metabolism were assessed using multivariate data analysis and multiexponential fitting of lifetime decays. Additional biomarkers for drug-specific responsiveness were retrieved through data-driven analyses. The results provide the potential to determine and optimize patient-specific treatment efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Simon Walz, Paul Pollehne, Ruizhi Geng, Johannes Schneider, Moritz Maas, Wilhelm K. Aicher, Arnulf Stenzl, Bastian Amend, Niklas Harland
Summary: This study explores the potential of establishing urine-derived tumor organoids from bladder cancer patients as an alternative to tissue-derived organoids. The researchers successfully cultured urine-derived BC organoids from 83% of the specimens investigated, with comparable efficiency to tissue-derived organoids. These urine-derived BC organoids showed similar expressions of BC markers compared to the parental tumor, suggesting their potential as a non-invasive tool for studying BC and evaluating therapeutic responses.
Article
Cell Biology
Simon Walz, Paul Pollehne, Philipp Vollmer, Wilhelm K. Aicher, Arnulf Stenzl, Niklas Harland, Bastian Amend
Summary: Different scaffolds have different effects on cell growth and protein expression in bladder cancer cells. BME is more effective in promoting organoid growth and protein expression.
Article
Biochemistry & Molecular Biology
Bastian Amend, Lea Buttgereit, Tanja Abruzzese, Niklas Harland, Harald Abele, Peter Jakubowski, Arnulf Stenzl, Raphael Gorodetsky, Wilhelm K. Aicher
Summary: The potential of using allogeneic placenta mesenchymal stromal cells (pMSCs) for regenerative therapy was investigated. The cells were successfully isolated from different term placentas and showed the ability to proliferate and express immune-regulatory cell surface marker CD276 in appropriate culture conditions. This suggests that allogeneic pMSC transplantations could be tolerated in clinical applications, especially for the treatment of degenerative disorders in elderly adults.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)