Article
Medicine, Research & Experimental
Somayeh Naderi, Zahra Shiri, Masoumeh Zarei-Kheirabadi, Sepideh Mollamohammadi, Parastoo Hosseini, Golnoosh Rahimi, Azadeh Moradmand, Azam Samadian, Amir Shojaei, Meghdad Yeganeh, Seyed Ahmad Mousavi, Motahare Badri, Adeleh Taei, Seyedeh-Nafiseh Hassani, Hossein Baharvand
Summary: This study aimed to generate transplantable midbrain dopaminergic (mDA) progenitors for treatment of Parkinson's Disease (PD). By optimizing the differentiation protocol using clinical-grade human embryonic stem cells (hESC), the researchers successfully obtained functional mDA progenitors. Transplantation of cryopreserved mDA progenitors into rats with 6-hydroxydopamine lesions resulted in functional recovery. This study provides a foundation for future translational applications.
Article
Cell Biology
Jingsi Yang, Nan Ding, Dandan Zhao, Yunsheng Yu, Chunlai Shao, Xuan Ni, Zhen-Ao Zhao, Zhen Li, Jianquan Chen, Zheng Ying, Miao Yu, Wei Lei, Shijun Hu
Summary: The study developed a new strategy of using EBSS treatment to mimic in vivo starvation event, promoting the maturation of hESC-CMs. Intermittent starvation through EBSS significantly enhanced structural, metabolic, and electrophysiological maturity of hESC-CMs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Shanti Sibuea, Joan K. Ho, Colin W. Pouton, John M. Haynes
Summary: The generation of midbrain dopaminergic neurons from pluripotent stem cells is promising for disease modelling and Parkinson's disease cell therapy. However, the impact of additives like dbcAMP, TGF beta 3, and DAPT on the phenotype of these neurons is unclear. This study shows that removing dbcAMP or TGF beta 3 significantly affects multiple markers of the dopaminergic phenotype, while removing DAPT affects other markers. These additives should be seen as phenotype-modifying rather than neuroprotective, and their presence in cultures may confound research results.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jinqi Wang, Xuanhao Zeng, Yating Liu, Weiling Lian, Haozhen Lv, Kelu Wei, Qi Zhang, Jinhua Xu
Summary: Embryonic stem cell-derived melanocytes have low immunogenicity in vitro and show potential for treating vitiligo.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Sofie Lautrup, Camilla Myrup Holst, Anne Yde, Stine Asmussen, Vibeke Thinggaard, Knud Larsen, Lisbeth Schmidt Laursen, Mette Richner, Christian B. Vaegter, G. Aleph Prieto, Nicole Berchtold, Carl W. Cotman, Tinna Stevnsner
Summary: DNA damage is a major contributor to aging, especially in the brain where reactive oxygen species can cause oxidative genomic damage. The base excision repair (BER) pathway plays a critical role in maintaining genome stability in the brain. However, the impact of aging on this pathway and the regulatory mechanisms involved are poorly understood. Using microarray analysis of human brain tissue, it was found that the expression of core BER genes is downregulated during aging across multiple brain regions. Moreover, the neurotrophin BDNF was identified as a potential regulator of BER, as its expression correlated with the expression of many BER genes.
Article
Engineering, Biomedical
Se Eun Jang, Lifeng Qiu, Xiaolei Cai, Jolene Wei Ling Lee, Wei Zhang, Eng-King Tan, Bin Liu, Li Zeng
Summary: The study demonstrated the biocompatibility of AIE-NPs in neuronal transplantation, showing high penetration into cells and intracellular long-term retention without affecting neuronal proliferation, differentiation, and viability. The findings highlight the potential application of AIE-NPs in neural grafting.
Article
Cell & Tissue Engineering
Jinghua Piao, Susan Zabierowski, Brittany N. Dubose, Ellen J. Hill, Monalisa Navare, Nidia Claros, Siera Rosen, Kiran Ramnarine, Callie Horn, Craig Fredrickson, Karen Wong, Brent Safford, Sonja Kriks, Abderrahman El Maarouf, Urs Rutishauser, Claire Henchcliffe, Yongzeng Wang, Isabelle Riviere, Shannon Mann, Vladimir Bermudez, Stefan Irion, Lorenz Studer, Mark Tomishima, Viviane Tabar
Summary: The study successfully generated midbrain dopamine neurons from human embryonic stem cells and conducted extensive tests on animals to demonstrate the efficacy and safety of the cell product intended for potential human use, paving the way for future clinical trials.
Article
Nanoscience & Nanotechnology
Baojie Guo, Yongchao Duan, Zhongwen Li, Yao Tian, Xuedi Cheng, Chunxu Liang, Wenjing Liu, Bin An, Wumei Wei, Tingting Gao, Shasha Liu, Xiyuan Zhao, Shuaishuai Niu, Chenxin Wang, Yukai Wang, Liu Wang, Guihai Feng, Wei Li, Jie Hao, Qi Gu, Qi Zhou, Jun Wu
Summary: The researchers developed a 21-day long-term serum-free culture system and successfully created cell sheets (CSs) with human embryonic stem cell-derived immunity-and-matrix-regulatory cells (IMRCs) that have stronger mechanical properties and greater extracellular matrix secretion capacity. These IMRC-CSs were effective in promoting corneal chemical injury repair and could be transformed into injectable temperature-sensitive hydrogels for uterine adhesion repair through a decellularization process.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Neurosciences
Nina M. Drager, Sydney M. Sattler, Cindy Tzu-Ling Huang, Olivia M. Teter, Kun Leng, Sayed Hadi Hashemi, Jason Hong, Giovanni Aviles, Claire D. Clelland, Lihong Zhan, Joe C. Udeochu, Lay Kodama, Andrew B. Singleton, Mike A. Nalls, Justin Ichida, Michael E. Ward, Faraz Faghri, Li Gan, Martin Kampmann
Summary: This study presents a screening platform to systematically elucidate the functional consequences of genetic perturbations in human induced pluripotent stem cell-derived microglia. The researchers identified genes controlling microglia survival, activation, and phagocytosis, as well as disease-associated genes. They also determined regulators of disease-relevant microglial states using single-cell RNA sequencing. The platform has the potential for therapeutic targeting and functional characterization of microglia.
NATURE NEUROSCIENCE
(2022)
Article
Endocrinology & Metabolism
Kim L. Keen, Andrew J. Petersen, Alexander G. Figueroa, Benjamin Fordyce, Jaeweon Shin, Rachita Yadav, Serkan Erdin, Robert A. Pearce, Michael E. Talkowski, Anita Bhattacharyya, Ei Terasawa
Summary: This study identified an efficient method for generating human GnRH neurons from stem cells and characterized their physiological characteristics. The research also demonstrated the similarity in developmental transcriptomes of GnRH neurons derived from human stem cells. These stem cell-derived GnRH neurons will be valuable for disease modeling and drug testing in reproductive disorders.
Article
Cell & Tissue Engineering
Rachel A. Heuer, Kevin T. Nella, Hsiang-Tsun Chang, Kyle S. Coots, Andrew M. Oleksijew, Christian B. Roque, Luisa H. A. Silva, Tammy L. McGuire, Kazuaki Homma, Akihiro J. Matsuoka
Summary: The study focuses on optimizing transplant conditions for human embryonic stem-cell-derived otic neuronal progenitor cells in spheroid form and suggests that transplantation after 7 days of 3D culture may be the most effective method. The findings indicate that the spheroids maintain characteristics similar to ONP cells and can potentially be a viable option for stem cell replacement therapies in the inner ear.
TISSUE ENGINEERING PART A
(2021)
Article
Cell & Tissue Engineering
Iina Korkka, Heli Skottman, Soile Nymark
Summary: Research shows that human embryonic stem cell (hESC)-derived retinal pigment epithelium (RPE) has functional potassium (K+) channels and ion channels, which are essential for its therapeutic effectiveness.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Veterinary Sciences
Bokyeong Ryu, Mi-Young Son, Kwang Bo Jung, Ukjin Kim, Jin Kim, Ohman Kwon, Ye Seul Son, Cho-Rok Jung, Jae-Hak Park, C-Yoon Kim
Summary: This study established hESC-ELCs as an intestinal toxicity model, showing a stronger correlation with the in vivo model compared to immortalized cell lines. This suggests that hESC-ELCs could serve as the next-generation model for intestinal toxicity assessment.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Neurosciences
Jacob B. Ruden, Mrinalini Dixit, Jose C. Zepeda, Brad A. Grueter, Laura L. Dugan
Summary: A new protocol has been developed to efficiently convert human iPSC-derived neural progenitor cells into mature neurons in a relatively short time frame. The mature neurons express functional NMDA receptors, demonstrating their normal functionality. This protocol has the potential to enhance the study of primate/human-specific aspects of NMDAR function and signaling in both health and disease.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Ieva Berzanskyte, Federica Riccio, Carolina Barcellos Machado, Elizabeth J. Bradbury, Ivo Lieberam
Summary: Researchers developed a method to differentiate and isolate spinal V3 interneurons using extrinsic factor-mediated differentiation and magnetic activated cell sorting. They found that a higher concentration of Sonic Hedgehog agonist and 3D culture conditions enhanced V3 progenitor differentiation. By developing a transgene reporter, they were able to purify V3 progenitors labeled with CD14 to a 95% purity and mature them into postmitotic V3 interneurons. This purification tool is valuable for in vitro disease modeling, studying normal human neural development, and potential cell therapies for spinal cord disorders.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Chenchen Song, Quyen B. Do, Srdjan D. Antic, Thomas Knopfel
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Neurosciences
Shaina M. Short, Katerina D. Oikonomou, Wen-Liang Zhou, Corey D. Acker, Marko A. Popovic, Dejan Zecevic, Srdjan D. Antic
JOURNAL OF NEUROPHYSIOLOGY
(2017)
Review
Neurosciences
Srdjan D. Antic, Michael Hines, William W. Lytton
JOURNAL OF NEUROSCIENCE RESEARCH
(2018)
Article
Biology
Anna R. Moore, Sarah E. Richards, Katelyn Kenny, Leandro Royer, Urann Chan, Kelly Flavahan, Stephen D. Van Hooser, Suzanne Paradis
Review
Neurosciences
Matthew T. Rich, Mary M. Torregrossa
BRAIN RESEARCH BULLETIN
(2018)
Article
Neurosciences
Mandakini B. Singh, Jesse A. White, Eric J. McKimm, Milena M. Milosevic, Srdjan D. Antic
Article
Neurosciences
Peter Quicke, Chenchen Song, Eric J. McKimm, Milena M. Milosevic, Carmel L. Howe, Mark Neil, Simon R. Schultz, Srdjan D. Antic, Amanda J. Foust, Thomas Knopfel
FRONTIERS IN CELLULAR NEUROSCIENCE
(2019)
Correction
Neurosciences
Peter Quicke, Chenchen Song, Eric J. McKimm, Milena M. Milosevic, Carmel L. Howe, Mark Neil, Simon R. Schultz, Srdjan D. Antic, Amanda J. Foust, Thomas Knopfel
FRONTIERS IN CELLULAR NEUROSCIENCE
(2019)
Article
Neurosciences
Dusica M. Kocovic, Pallavi V. Limaye, Lauren C. H. Colburn, Mandakini B. Singh, Milena M. Milosevic, Jasmina Tadic, Milos Petronijevic, Svetlana Vrzic-Petronijevic, Pavle R. Andjus, Srdjan D. Antic
Editorial Material
Neurosciences
Srdjan D. Antic, Bradley James Baker, Marco Canepari
FRONTIERS IN CELLULAR NEUROSCIENCE
(2019)
Article
Neurosciences
Matthew T. Rich, Yanhua H. Huang, Mary M. Torregrossa
JOURNAL OF NEUROSCIENCE
(2020)
Article
Neurosciences
Sarah E. Richards, Anna R. Moore, Alice Y. Nam, Shikhar Saxena, Suzanne Paradis, Stephen D. Van Hooser
JOURNAL OF NEUROSCIENCE
(2020)
Article
Neurosciences
Milena M. Milosevic, Jinyoung Jang, Eric J. McKimm, Mei Hong Zhu, Srdjan D. Antic
Article
Neurosciences
Craig Kelley, Salvador Dura-Bernal, Samuel A. Neymotin, Srdjan D. Antic, Nicholas T. Carnevale, Michele Migliore, William W. Lytton
Summary: Pyramidal neurons in the neocortex have complex input-output relationships that cannot be captured by simple models. Experimental studies have shown that dendritic impedance properties in these neurons depend on various factors and can be partially replicated by different biophysically detailed models, with the best fit incorporating HCN and TASK-like channels. These channels interact to shape the impedance and voltage responses in the dendrites of these neurons.
JOURNAL OF NEUROPHYSIOLOGY
(2021)
Article
Neurosciences
Craig Kelley, Srdjan D. Antic, Nicholas T. Carnevale, John L. Kubie, William W. Lytton
Summary: This study aims to develop an analysis method to evaluate the nonlinear responses of neuronal membranes and explore the phase relationships between rhythmic stimuli and subthreshold and spiking membrane potential responses. The results suggest that cortical neurons exhibit differential responses to oscillatory inputs depending on the frequency, which plays a role in neuronal responses to shifts in network state.
JOURNAL OF NEUROPHYSIOLOGY
(2023)