Article
Cell Biology
Baojiang Wu, Yanqiu Wang, Xinhua Wei, Jingcheng Zhang, Jiahui Wu, Guifang Cao, Yong Zhang, Jun Liu, Xihe Li, Siqin Bao
Summary: The overexpression of Nelfa enhances the conversion of mouse ESCs into 2C-like cells and improves somatic cell reprogramming efficiency. Co-overexpression of Nelfa and Bcl2 activates the 2C-like state in ESCs and gives the cells dual cell fate potential. These findings provide insights into the roles of Nelfa and Bcl2 in the establishment and regulation of the totipotent state in mouse ESCs.
CELL PROLIFERATION
(2023)
Article
Biotechnology & Applied Microbiology
Aliosha I. Figueroa-Valdes, Catalina de la Fuente, Yessia Hidalgo, Ana Maria Vega-Letter, Rafael Tapia-Limonchi, Maroun Khoury, Francisca Alcayaga-Miranda
Summary: Cell therapy is shifting towards cell-free treatments using small extracellular vesicles (sEV) that mimic the effects of parental cells, but faces challenges in large-scale production and regulation compliance. Using a regulatory-compliant medium like Oxium(TM)EXO can significantly increase sEV secretion rates and improve production efficiency.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Anna M. Kolkmann, Anon Van Essen, Mark J. Post, Panagiota Moutsatsou
Summary: This study aimed to develop a defined, cost-effective serum-free and animal-free medium for the attachment and expansion of bovine myoblasts. A serum-free medium composition was developed based on a comprehensive literature analysis and it supported exponential cell growth up to 97% of the serum-containing golden standard growth medium. This is the first defined serum-free and animal-free medium formulation specific for bovine myoblasts to date.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Yun Chang, Ramizah Syahirah, Stephanie N. Oprescu, Xuepeng Wang, Juhyung Jung, Scott H. Cooper, Sandra Torregrosa-Allen, Bennett D. Elzey, Alan Y. Hsu, Lauren N. Randolph, Yufei Sun, Shihuan Kuang, Hal E. Broxmeyer, Qing Deng, Xiaojun Lian, Xiaoping Bao
Summary: This study demonstrates the successful generation of AGM-like hematopoietic system from human pluripotent stem cells through temporal manipulation of Wnt signaling. The generated hematopoietic progenitor cells resemble primary cord blood HSCs at the transcriptional level and exhibit lymphoid and myeloid potential in vitro. The cells also show the ability to home to a definitive hematopoietic site, rescue bloodless zebrafish, and engraft and repopulate in mouse recipients.
Article
Biochemistry & Molecular Biology
Sergio Cortez Ghio, Martin A. Barbier, Emilie J. Doucet, Imad Debbah, Meryem Safoine, Gaetan Le-Bel, Andreanne Cartier, Emilie Jolibois, Amelie Morissette, Danielle Larouche, Julie Fradette, Sylvain L. Guerin, Alain Garnier, Lucie Germain
Summary: We developed a new chemically defined serum-free medium, Surge SFM, which can support massive expansion of human primary keratinocytes and production of skin substitutes. Compared to conventional serum-containing medium, Surge SFM can more efficiently expand keratinocytes and properly form stratified epidermis. It also retains keratin-19-positive epithelial stem cells and maintains similar gene expression as with the conventional medium. Surge SFM can be used to produce bilayered self-assembled skin substitutes histologically similar to those produced using serum-containing medium, which can persist for up to six months when grafted onto mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Yanqiu Wang, Qin Na, Xihe Li, Wee-Wei Tee, Baojiang Wu, Siqin Bao
Summary: Retinoic acid induced a NELFA-mediated 2C-like state in mouse ESCs, characterized by 2C-specific transcriptional networks and the ability to contribute trophectoderm (TE) when injected into developing embryos. RA treatment also triggers DNA hypomethylation, active histone modification, suppressed glycolysis metabolism, and reduced protein synthesis activity of ESCs, indicating a broader role in 2C-like cells state.
CELL PROLIFERATION
(2021)
Article
Reproductive Biology
Matej Murin, Lucie Nemcova, Alexandra Bartkova, Ahmed Gad, Andrea Lucas-Hahn, Frantisek Strejcek, Radek Prochazka, Jozef Laurincik
Summary: The previous strong support for the statement that fully-grown porcine oocytes are transcriptionally quiescent is now challenged. A new study reveals differences in the transcription profiles between germinal vesicle (GV) and metaphase II (MII) oocytes. The study compares the transcription profiles of oocytes at different stages and identifies differentially transcribed mRNAs that are important for key processes during in vitro maturation.
Article
Biochemical Research Methods
Narae Kim, Yohei Yokobayashi
Summary: Mutated VSV vectors show improved stability and lower cytopathic effects in ESCs, allowing for chemical regulation of transgene expression through embedded riboswitches, maintaining pluripotency of cells and enabling the production of various differentiated cells without chromosomal alteration.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Cell Biology
Olena A. Kolesnichenko, Hannah M. Flood, Yufang Zhang, Vladimir Ustiyan, Hayde Cuervo K. Jimenez, Tanya V. Kalin, Vladimir V. Kalinichenko
Summary: Vascular remodeling and compromised alveolar development are key aspects of chronic pulmonary diseases. Restoring endothelial progenitor cells (EPCs) through transplantation can stimulate neonatal angiogenesis. By culturing c-KIT(+)FOXF1(+) EPCs derived from pluripotent ESCs and transplanting them into hyperoxia-injured mice, a proof-of-principle was provided that ESC-derived c-KIT(+)FOXF1(+) EPCs can prevent alveolar simplification.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell & Tissue Engineering
Xiaojie Hou, Shuhong Ma, Wei Fan, Fang Li, Miaomiao Xu, Chao Yang, Feng Liu, Ying Yan, Juyi Wan, Feng Lan, Bin Liao
Summary: In this study, a chemically defined method for generating SANLCs was established through combined modulation of WNT, RA, and FGF signaling pathways and metabolic selection. This method may serve as a platform for disease modeling, drug discovery, predictive toxicology, and biological pacemaker construction.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Richard Biener, Thomas Horn, Alexander Komitakis, Ines Schendel, Leon Koenig, Anna Hauenstein, Alina Ludl, Andrea Speidel, Svenja Schmid, Julian Weisser, Max Brossmann, Sofia Kern, Max Kronmueller, Sonja Vierkorn, Lennart Suckow, Arthur Braun
Summary: V. natriegens is a halophilic bacterium with the fastest generation time reported so far. A low-chloride chemically defined medium has been developed to achieve high-cell-density cultivation and prevent corrosion on metal surfaces.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Bowen Zhang, Xumin Wu, Guicheng Zi, Lijuan He, Sihan Wang, Lin Chen, Zeng Fan, Xue Nan, Jiafei Xi, Wen Yue, Lei Wang, Liu Wang, Jie Hao, Xuetao Pei, Yanhua Li
Summary: By optimizing the culture protocol, a large number of megakaryocytes were successfully generated from human embryonic stem cells, confirming their biosafety and demonstrating their ability to form platelets. This study further enhances the clinical applications of megakaryocytes or platelets derived from pluripotent stem cells.
CELL PROLIFERATION
(2021)
Article
Cell Biology
Stefano Panella, Francesco Muoio, Valentin Jossen, Yves Harder, Regine Eibl-Schindler, Tiziano Tallone
Summary: Adipose tissue is a rich source of stem cells, but liposuction cannot provide enough for direct applications in regenerative medicine. Hence, GMP-compliant ex vivo expansion protocols are needed to ensure safe, reproducible, and cost-effective production of cell drugs. The developed UrSuppe cell culture medium allows for the cultivation and differentiation of human adipose stem cells, potentially facilitating the translation of research projects into clinical applications.
Article
Agriculture, Dairy & Animal Science
Josep M. Cambra, Emilio A. Martinez, Heriberto Rodriguez-Martinez, Maria A. Gil, Cristina Cuello
Summary: The study evaluated the transcriptome of in vitro-produced pig embryos cultured in defined and undefined media, showing that culture conditions strongly affect the embryo transcriptome. Although no significant differences were found between in vitro-produced embryos, many altered genes were observed when compared with in vivo-derived embryos.
Article
Biotechnology & Applied Microbiology
Ji-Hye Seo, Young-Joo Jeon
Summary: This study aimed to establish an effective novel therapeutic cellular source from human embryonic stem cells (hESCs) and investigate its molecular expression profile. The results indicated that the derived mesenchymal stem cells have high proliferative capacity, potentially interact with the Wnt/beta-catenin pathway, and offer higher therapeutic potential.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)