Article
Multidisciplinary Sciences
Yang Yang, Maria Gomez, Timothy Marsh, Laura Poillet-Perez, Akshada Sawant, Lei Chen, Noel R. Park, S. RaElle Jackson, Zhixian Hu, Noa Alon, Chen Liu, Jayanta Debnath, Jun-Lin Guan, Shawn Davidson, Michael Verzi, Eileen White
Summary: Autophagy defects are a risk factor for inflammatory bowel diseases (IBDs) and affect unknown mechanisms. Conditional deletion of specific genes Atg5 or Fip200/Atg17 causes death in mice through autophagy inhibition, loss of barrier function, and elimination of stem cells. Autophagy plays a crucial role in maintaining mesenchymal cell metabolism and intestinal stem cell renewal.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Developmental Biology
Yi Jacky Peng, Xinyu Thomas Tang, Hui Sophie Shu, Wenjie Dong, Hongfang Shao, Bo O. Zhou
Summary: The study found that testicular stromal cells, including Sertoli, endothelial, Leydig, smooth muscle, and Tcf21-CreER+ stromal cells, express stem cell factor (SCF), an essential growth factor for spermatogenesis. Undifferentiated and differentiating spermatogonia were associated with SCF-expressing Sertoli cells. Conditional deletion of SCF from Sertoli cells blocked the differentiation of spermatogonia, leading to male infertility. Conditional overexpression of SCF in Sertoli cells significantly increased spermatogenesis.
Article
Medicine, General & Internal
Hui Shen, Jun Han, Chunlei Liu, Fei Cao, Yijuan Huang
Summary: Grape seed proanthocyanidins (GSPs) have radioprotective effects, reducing damage to the testes and intestines caused by ionizing radiation and protecting their functions. This study provides a potential option for the development of radioprotective drugs.
FRONTIERS IN MEDICINE
(2022)
Article
Cell & Tissue Engineering
Ankita Kaushik, Deepa Bhartiya
Summary: This study investigates the development of testicular germ cell tumors (T2GCT) in mice exposed to diethylstilbestrol (DES) during early life. It reveals that neonatal DES exposure affects testicular stem cells and leads to infertility, reduced sperm counts, and tumor-like changes. The study also highlights the potential of the mouse model for understanding cancer initiation, cancer stem cells, and developing effective therapies.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cell & Tissue Engineering
Zijun Cai, Di Liu, Yuntao Yang, Wenqing Xie, Miao He, Dengjie Yu, Yuxiang Wu, Xiuhua Wang, Wenfeng Xiao, Yusheng Li
Summary: This article reviews the relationship between sarcopenia and stem cells, discussing the roles of satellite cells (SCs) and muscle-derived stem cells (MDSCs) in sarcopenia. Studies have shown that changes in the number and function of stem cells can trigger sarcopenia and have adverse effects on stem cells. A better understanding of the role of stem cells, especially SCs and MDSCs, in muscle, is important for developing novel therapeutic approaches to combat sarcopenia.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biology
Leah J. Greenspan, Margaret de Cuevas, Kathy H. Le, Jennifer M. Viveiros, Erika L. Matunis
Summary: Adult stem cells in the Drosophila testis are regulated by signals from somatic hub cells, including the Epidermal growth factor receptor (EGFR) signaling pathway. After the removal of somatic cyst stem cells (CySCs), the hub cells proliferate and undergo transdifferentiation into new CySCs. EGFR signaling is normally inhibited in hub cells but is activated after CySC ablation to drive stem cell regeneration.
Article
Biochemistry & Molecular Biology
Ralfs Buks, Megane Brusson, Sylvie Cochet, Tatiana Galochkina, Bruno Cassinat, Ivan Nemazanyy, Thierry Peyrard, Jean-Jacques Kiladjian, Alexandre G. de Brevern, Slim Azouzi, Wassim El Nemer
Summary: MPNs are characterized by abnormal clonal expansion of hematopoietic stem cells, leading to a high risk of thrombosis and progression to myelofibrosis and leukemia. ABCG2 may play a role in multidrug resistance in MPNs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Wei Yang, Li-Bo Liu, Feng-Liang Liu, Yan-Hua Wu, Zi-Da Zhen, Dong-Ying Fan, Zi-Yang Sheng, Zheng-Ran Song, Jia-Tong Chang, Yong-Tang Zheng, Jing An, Pei-Gang Wang
Summary: Through single-cell RNA sequencing of ZIKV-infected mouse testes, it is found that spermatogenic cells, especially spermatogonia, are vulnerable to ZIKV infection. The complement system genes are significantly upregulated in infiltrated S100A4 + monocytes/macrophages. Complement activation and testicular damage are verified in ZIKV-infected macaques and the complement inhibitor C1INH and S100A4 inhibitors sulindac and niclosamide are tested. C1INH alleviates testicular pathology but worsens ZIKV infection, while niclosamide effectively reduces infiltration, inhibits complement activation, alleviates testicular damage, and improves fertility. This discovery highlights the importance of male reproductive health protection during future ZIKV epidemics.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Yuki Ohashi, Masao Toyoda, Nobumichi Saito, Masahiro Koizumi, Genta Kanai, Hirotaka Komaba, Moritsugu Kimura, Takehiko Wada, Hiroo Takahashi, Yuichiro Takahashi, Naoto Ishida, Takatoshi Kakuta, Masafumi Fukagawa, Kimiyoshi Ichida
Summary: Two-thirds of urate is excreted via the renal pathway and the remaining one-third mainly via the intestine. We evaluated the capacity of extra-renal ABCG2 in anuric hemodialysis patients and found that it could excrete up to approximately 60% of the daily uric acid turnover. This highlights the importance of the extra-renal pathway in uric acid homeostasis for patients with renal dysfunction.
SCIENTIFIC REPORTS
(2023)
Article
Nutrition & Dietetics
Lei Liu, Tianyi Zhao, Lizhen Shan, Ling Cao, Xiaoxia Zhu, Yu Xue
Summary: This study revealed that estradiol regulates intestinal ABCG2 through the PI3K/Akt pathway to promote urate excretion, thereby reducing serum urate levels.
NUTRITION & METABOLISM
(2021)
Review
Cell Biology
Kuang-Qi Chen, Bang -Hong Wei, Shuang-Li Hao, Wan -Xi Yang
Summary: The PI3K/AKT signaling pathway plays a crucial role in regulating cell proliferation, survival, and anti-apoptosis in animal cells. In the seminiferous epithelium, it has been shown to regulate the proliferation and survival of immature Sertoli cells and spermatogenic cells, as well as the structure of the blood-testis barrier in mature Sertoli cells, thereby directly and indirectly maintaining and promoting spermatogenesis in the male testis.
HISTOLOGY AND HISTOPATHOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Sara E. Cloft, Zehava Uni, Eric A. Wong
Summary: Mature small intestines have crypts populated by stem cells, which produce replacement cells for maintaining the absorptive villus surface area. The expression of stem cell markers Olfactomedin 4 (Olfm4) and Leucine-rich repeat containing G protein-coupled receptor 5 (Lgr5) differed after hatching, with Olfm4 mRNA increasing and Lgr5 mRNA decreasing. Marker of proliferation Ki67 mRNA decreased post-hatch in the duodenum but was greatest in the ileum. The ontogeny of Olfm4, Lgr5, and Ki67 expressing cells shows that proliferation in the peri-hatch intestine changes from along the entire villi to being restricted within the crypts.
Article
Chemistry, Multidisciplinary
Kai Xia, Fulin Wang, Zhipeng Tan, Suyuan Zhang, Xingqiang Lai, Wangsheng Ou, Cuifeng Yang, Hong Chen, Hao Peng, Peng Luo, Anqi Hu, Xiang'an Tu, Tao Wang, Qiong Ke, Chunhua Deng, Andy Peng Xiang
Summary: Genetically corrected stem Leydig cells transplantation effectively restores sexual function and allows for reproduction in a mouse model of hereditary primary hypogonadism (HPH), indicating the potential of this approach for HPH therapy.
Article
Geriatrics & Gerontology
Rebecca E. Schmitt, Aneesha Dasgupta, Paige C. Arneson-Wissink, Srijani Datta, Alexandra M. Ducharme, Jason D. Doles
Summary: Up to half of sepsis survivors suffer from chronic critical illness (CCI), which includes multiple physical and mental disabilities that hinder their return to regular activities. A study has shown that muscle stem cells (MuSCs) and muscle regeneration play crucial roles in post-sepsis muscle recovery and that sepsis triggers morphological, functional, and transcriptional changes in MuSCs.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Review
Biochemistry & Molecular Biology
Korin Sahinyan, Felicia Lazure, Darren M. Blackburn, Vahab D. Soleimani
Summary: This review discusses the factors and effects of declining numbers and regenerative potential of muscle stem cells (MuSCs) during aging, and emphasizes the importance of understanding these changes for developing effective therapies for age-related muscle defects.
Article
Biotechnology & Applied Microbiology
Sheng Zhou, Melissa A. Bonner, Yong-Dong Wang, Samuel Rapp, Suk See De Ravin, Harry L. Malech, Brian P. Sorrentino
HUMAN GENE THERAPY METHODS
(2015)
Article
Cell Biology
Suk See De Ravin, Xiaolin Wu, Susan Moir, Sandra Anaya-O'Brien, Nana Kwatemaa, Patricia Littel, Narda Theobald, Uimook Choi, Ling Su, Martha Marquesen, Dianne Hilligoss, Janet Lee, Clarissa M. Buckner, Kol A. Zarember, Geraldine O'Connor, Daniel McVicar, Douglas Kuhns, Robert E. Throm, Sheng Zhou, Luigi D. Notarangelo, I. Celine Hanson, Mort J. Cowan, Elizabeth Kang, Coleen Hadigan, Michael Meagher, John T. Gray, Brian P. Sorrentino, Harry L. Malech
SCIENCE TRANSLATIONAL MEDICINE
(2016)
Article
Hematology
Soghra Fatima, Sheng Zhou, Brian P. Sorrentino
EXPERIMENTAL HEMATOLOGY
(2018)
Article
Medicine, General & Internal
Ewelina Mamcarz, Sheng Zhou, Timothy Lockey, Hossam Abdelsamed, Shane J. Cross, Guolian Kang, Zhijun Ma, Jose Condori, Jola Dowdy, Brandon Triplett, Chen Li, Gabriela Maron, Juan C. Aldave Becerra, Joseph A. Church, Elif Dokmeci, James T. Love, Ana C. da Matta Ain, Hedi van der Watt, Xing Tang, William Janssen, Byoung Y. Ryu, Suk See De Ravin, Mitchell J. Weiss, Benjamin Youngblood, Janel R. Long-Boyle, Stephen Gottschalk, Michael M. Meagher, Harry L. Malech, Jennifer M. Puck, Morton J. Cowan, Brian P. Sorrentino
NEW ENGLAND JOURNAL OF MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Yoonjeong Jang, Yoon-Sang Kim, Matthew M. Wielgosz, Francesca Ferrara, Zhijun Ma, Jose Condori, Lance E. Palmer, Xiwen Zhao, Guolian Kang, David J. Rawlings, Sheng Zhou, Byoung Y. Ryu
Article
Medicine, Research & Experimental
Matthew Bauler, Jessica K. Roberts, Chang-Chih Wu, Baochang Fan, Francesca Ferrara, Bon Ham Yip, Shiyong Diao, Young-In Kim, Jennifer Moore, Sheng Zhou, Matthew M. Wielgosz, Byoung Ryu, Robert E. Throm
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2020)
Article
Oncology
Zelda Ode, Jose Condori, Nicolas Peterson, Sheng Zhou, Giedre Krenciute
Article
Medicine, Research & Experimental
Janice M. Riberdy, Sheng Zhou, Fei Zheng, Young-In Kim, Jennifer Moore, Abishek Vaidya, Robert E. Throm, April Sykes, Natasha Sahr, Challice L. Bonifant, Byoung Ryu, Stephen Gottschalk, Mireya Paulina Velasquez
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2020)
Article
Oncology
Phuong Nguyen, Emmanuel Okeke, Michael Clay, Dalia Haydar, Julie Justice, Carla O'Reilly, Shondra Pruett-Miller, James Papizan, Jennifer Moore, Sheng Zhou, Robert Throm, Giedre Krenciute, Stephen Gottschalk, Christopher DeRenzo
MOLECULAR THERAPY-ONCOLYTICS
(2020)
Article
Medicine, Research & Experimental
Melissa A. Bonner, Antonio Morales-Hernandez, Sheng Zhou, Zhijun Ma, Jose Condori, Yong-Dong Wang, Soghra Fatima, Lance E. Palmer, Laura J. Janke, Stephanie Fowler, Brian P. Sorrentino, Shannon McKinney-Freeman
Summary: Vector-mediated mutagenesis remains a major safety concern for many gene therapy clinical protocols, but prolonged ectopic expression of HMGA2 in non-human primate HSCs is not sufficient to drive hematologic malignancy.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Young-In Kim-Hoehamer, Janice M. Riberdy, Fei Zheng, Jeoungeun J. Park, Na Shang, Jean-Yves Metais, Timothy Lockey, Catherine Willis, Salem Akel, Jennifer Moore, Michael M. Meagher, M. Paulina Velasquez, Brandon M. Triplett, Aimee C. Talleur, Stephen Gottschalk, Sheng Zhou
Summary: We have successfully developed a current good manufacturing practice-compliant process to manufacture allogeneic CD19-CAR T cells, which have shown potent antitumor activity in vitro and in vivo.
Article
Cell Biology
Brooke Prinzing, Caitlin C. Zebley, Christopher T. Petersen, Yiping Fan, Alejandro Allo Anido, Zhongzhen Yi, Phuong Nguyen, Haley Houke, Matthew Bell, Dalia Haydar, Charmaine Brown, Shannon K. Boi, Shanta Alli, Jeremy Chase Crawford, Janice M. Riberdy, Jeoungeun J. Park, Sheng Zhou, Mireya Paulina Velasquez, Chris DeRenzo, Cicera R. Lazzarotto, Shengdar Q. Tsai, Peter Vogel, Shondra M. Pruett-Miller, Deanna M. Langfitt, Stephen Gottschalk, Ben Youngblood, Giedre Krenciute
Summary: CAR T cell exhaustion occurs due to epigenetic repression of T cell multipotent potential, deletion of DNMT3A enhances CAR T cell functionality and upregulates interleukin-10, and a genome-wide DNA methylation profiling identifies genes targeted for epigenetic silencing that limits immune cell stemness. This epigenetically regulated multipotency program is crucial for the clinical outcome of CAR T cell therapies, highlighting the importance of leveraging epigenetic mechanisms to improve CAR T cell efficacy.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Hematology
Aimee C. Talleur, Amr Qudeimat, Jean-Yves Metais, Deanna Langfitt, Ewelina Mamcarz, Jeremy Chase Crawford, Sujuan Huang, Cheng Cheng, Caitlin Hurley, Renee Madden, Akshay Sharma, Ali Suliman, Ashok Srinivasan, M. Paulina Velasquez, Esther A. Obeng, Catherine Willis, Salem Akel, Seth E. Karol, Hiroto Inaba, Allison Bragg, Wenting Zheng, Sheng M. Zhou, Sarah Schell, Macal Tuggle-Brown, David Cullins, Sagar L. Patil, Ying Li, Paul G. Thomas, Caitlin Zebley, Benjamin Youngblood, Ching-Hon Pui, Timothy Lockey, Terrence L. Geiger, Michael M. Meagher, Brandon M. Triplett, Stephen Gottschalk
Summary: CD19-specific CAR T cells have potent antileukemia activity in pediatric and adult B-ALL patients, but not all patients achieve a complete response. This clinical study evaluated a new CD19-CAR T cell product in pediatric patients and showed that the treatment was safe and effective, with disease burden before infusion affecting the treatment outcome.
Article
Multidisciplinary Sciences
Suk See De Ravin, Siyuan Liu, Colin L. Sweeney, Julie Brault, Narda Whiting-Theobald, Michelle Ma, Taylor Liu, Uimook Choi, Janet Lee, Sandra Anaya O'Brien, Priscilla Quackenbush, Tyra Estwick, Anita Karra, Ethan Docking, Nana Kwatemaa, Shuang Guo, Ling Su, Zhonghe Sun, Sheng Zhou, Jennifer Puck, Morton J. Cowan, Luigi D. Notarangelo, Elizabeth Kang, Harry L. Malech, Xiaolin Wu
Summary: De Ravin et al. report on the unintended consequences of lentiviral gene therapy in a clinical trial for the treatment of X-linked Severe Combined Immunodeficiency. They found that vector-induced alternative splicing events led to clonal dominance and clonal expansion. However, this issue can be mitigated by removing the splice acceptor. These findings highlight the importance of functional analysis of lentivectors for preclinical safety assessment and a redesign of clinical vectors to improve safety.
NATURE COMMUNICATIONS
(2022)
Article
Cell & Tissue Engineering
Salem Akel, Leigh Poston, Jeoungeun J. Park, Susan B. Schoultz, Lina Alloush, Fei Zheng, Sheng Zhou, Timothy Lockey, Catherine Willis, Christopher DeRenzo, Stephen Gottschalk
Summary: A simple thaw/wash procedure was developed and validated in this study for the preparation of B7-H3-CAR T cells for locoregional delivery. The quality, safety attributes, and stability of the products were assessed and met the desired specifications within 3 hours post thaw/wash, demonstrating the effectiveness of the procedure.