Article
Multidisciplinary Sciences
Ana C. Delgado, Angel R. Maldonado-Soto, Violeta Silva-Vargas, Dogukan Mizrak, Thomas von Kanel, Kelly R. Tan, Alex Paul, Aviv Madar, Henar Cuervo, Jan Kitajewski, Chyuan-Sheng Lin, Fiona Doetsch
Summary: Adult neural stem cells in the ventricular-subventricular zone of mice can generate glial cells in addition to neurons, with certain domains remaining quiescent under normal conditions. Our study also uncovers an intraventricular oligodendrocyte progenitor derived from NSCs that is recruited upon injury.
Article
Biochemistry & Molecular Biology
Mikhail Semenov
Summary: The study focused on cell proliferation in the postnatal mouse brain, identifying four compartments with different densities of proliferating cells. It estimated the number of new cells produced in each compartment and concluded that adult neurogenesis only supports compensatory regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Bilal El Waly, Claire Bertet, Mathilde Paris, Marie Falque, Pierre Milpied, Karine Magalon, Myriam Cayre, Pascale Durbec
Summary: In this study, the researchers investigated the spontaneous fate conversion of neuronal progenitors in the adult mouse sub-ventricular zone (SVZ) after demyelinating insult. They found that the neuroblasts converted into oligodendrocytes that participate in the remyelination process. Through in vivo cell lineage and single cell RNA-sequencing, they demonstrated that the fate conversion proceeds through the formation of a non-proliferating transient cellular state expressing markers of both neuronal and oligodendrocyte identities. The conversion involves the stepwise upregulation/downregulation of key TFs and chromatin modifiers, as well as fine adjustments of transcription and translation machineries, metabolism, and migratory behaviors. This is the first in-depth analysis of a spontaneous cell fate conversion in the adult mammalian central nervous system (CNS).
Article
Biochemical Research Methods
Ahmed K. M. A. Ahmed, Toke Jost Isaksen, Toshihide Yamashita
Summary: This protocol outlines a straightforward method for isolating, culturing, and differentiating adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells serve as a valuable in vitro model for studying stem cell properties and plasticity in the adult brain. For detailed instructions on utilizing this protocol, refer to Isaksen et al. (2020).
Article
Genetics & Heredity
Dimo S. Stoyanov, Martin N. Ivanov, Tetsumori Yamashima, Anton B. Tonchev
Summary: The Zbtb20 gene is expressed in the adult primate anterior subventricular zone and rostral migratory stream, where it co-labels with neural stem cells, neuroblasts, and astrocytes. It has also been shown to be upregulated in response to ischemia, suggesting its involvement in neurogenesis. This research highlights the importance of ZBTB20 in mammalian cortical development and opens avenues for further functional studies in primates.
Article
Neurosciences
Daniel Esteve, Maria Micaela Molina-Navarro, Esther Giraldo, Noelia Martinez-Varea, Mari-Carmen Blanco-Gandia, Marta Rodriguez-Arias, Jose Manuel Garcia-Verdugo, Jose Vina, Ana Lloret
Summary: Neurogenesis and neuronal migration in the adult brain are impaired in Alzheimer's disease, leading to cell senescence and reduced odor discrimination ability. Studying the APP/PS1 Alzheimer's mouse model may provide insights for new Alzheimer's treatments based on senolytic compounds or pro-neurogenic factors.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ali Ebrahimi, Kazem Parivar, Nasim Hayati-e Roodbari, Akram Eidi
Summary: This study investigated the effects of quercetin on the expression of the Nrf2 gene and the proliferation and differentiation of NPSCs derived from the brain of adult rats. The results showed that quercetin increased the expression of Nrf2 and had a dose-dependent effect on the proliferation and differentiation of NPSCs. These findings suggest that quercetin may be an effective compound for treating degenerative CNS disorders.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Cell Biology
Fabrizio Favaloro, Annina M. DeLeo, Ana C. Delgado, Fiona Doetsch
Summary: Neural stem cells in the adult ventricular-subventricular zone play a crucial role in generating neurons and glia. MicroRNAs, specifically miR-17 and miR-92, are found to be highly upregulated in the activated stem cells and transit amplifying cells. Their deletion has stage-specific effects, affecting proliferation and differentiation of various subpopulations within the adult ventricular-subventricular zone. These findings highlight the importance of miR-17 and miR-92 in regulating neural stem cell function.
Article
Multidisciplinary Sciences
Gabriel S. Jensen, Jake W. Willows, David T. Breault, Kristy L. Townsend
Summary: A telomerase reverse transcriptase (Tert) lineage-tracing mouse line was developed to investigate the behavior and fate of adult tissue stem cells. By administering the tetracycline derivative doxycycline, cells expressing Tert can be permanently marked and tracked using specific markers, allowing for the study of stem cell activation, proliferation, migration, and differentiation.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Biochemical Research Methods
Thomas I-H Park, Leon C. D. Smyth, Miranda Aalderink, Zoe R. Woolf, Justin Rustenhoven, Kevin Lee, Deidre Jansson, Amy Smith, Sheryl Feng, Jason Correia, Peter Heppner, Patrick Schweder, Edward Mee, Mike Dragunow
Summary: This article presents a simple and highly reproducible method to culture various types of human brain cells from brain specimens. The method allows for quick preparation of cells and can be used to study human brain cells.
Article
Neurosciences
Minseon Park, William Baker, Dilraj Cambow, Danielle Gogerty, Ana Rachel Leda, Bridget Herlihy, Darya Pavlenko, Schuyler van den Nieuwenhuizen, Michal Toborek
Summary: Exposure to METH combined with HIV infection can induce aberrant proliferation of SVZ-derived NPCs through the CXCL12-CXCR4-Akt-1-mediated phosphorylation of FOXO3.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Weipang Chang, Andrea Pedroni, Maria Bertuzzi, Caghan Kizil, Andras Simon, Konstantinos Ampatzis
Summary: The study shows that physical exercise can activate neurogenesis in the spinal cord of adult zebrafish through cholinergic neurotransmission and a reduction of GABA(A) receptors. The researchers also found that functional recovery after spinal cord injury can be promoted by stimulating cholinergic receptors and interfering with GABAergic signaling. The findings suggest a model for activity-dependent neurogenesis in the adult zebrafish spinal cord during homeostasis and regeneration.
NATURE COMMUNICATIONS
(2021)
Review
Cell Biology
Milena Tucic, Vera Stamenkovic, Pavle Andjus
Summary: TnC plays a crucial role in regulating cell proliferation and migration during adult neurogenesis, with implications for regenerative therapies. Understanding ECM signaling in the CNS niche is essential for potential therapeutic applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Shaopeng Chi, Yaxiong Cui, Haiping Wang, Jinghui Jiang, Tingxin Zhang, Suhua Sun, Zhuan Zhou, Yi Zhong, Bailong Xiao
Summary: This study reveals the importance of mechanotransduction mediated by Piezo1 in astrocytes for adult neurogenesis and cognitive functions. Piezo1 in astrocytes mediates mechanically evoked calcium responses and ATP release, and its deficiency leads to severe impairment in brain structure and function.
Article
Toxicology
Nadja Spitzer, Kay-Cee K. Patterson, Daniel W. Kipps
Summary: Environmental exposure to low-level AgNPs may lead to physiological changes in brain cells through mechanisms involving disruption of f-actin organization, neurite collapse, and interaction with pAkt signaling. Understanding these mechanisms is important for regulating the use and disposal of AgNPs.
TOXICOLOGY IN VITRO
(2021)
Article
Biochemistry & Molecular Biology
Ergin Beyret, Hsin-Kai Liao, Mako Yamamoto, Reyna Hernandez-Benitez, Yunpeng Fu, Galina Erikson, Pradeep Reddy, Juan Carlos Izpisua Belmonte
Article
Cell & Tissue Engineering
Lorena Cortes-Medina, Herminia Pasantes-Morales, Alejandro Aguilera-Castrejon, Arturo Picones, Cesar O. Lara-Figueroa, Enoch Luis, Juan Jose Montesinos, Victor A. Cortes-Morales, M. P. De la Rosa Ruiz, Erika Hernandez-Estevez, Laura C. Bonifaz, Marco Antonio Alvarez-Perez, Gerardo Ramos-Mandujano
STEM CELLS INTERNATIONAL
(2019)
Review
Biochemistry & Molecular Biology
Khaled Alsayegh, Lorena Cortes-Medina, Gerardo Ramos-Mandujano, Heba Badraiq, Mo Li
Letter
Cell Biology
Paloma Martinez-Redondo, Isabel Guillen-Guillen, Noah Davidsohn, Chao Wang, Javier Prieto, Masakazu Kurita, Fumiyuki Hatanaka, Cuiqing Zhong, Reyna Hernandez-Benitez, Tomoaki Hishida, Takashi Lezaki, Akihisa Sakamoto, Amy N. Nemeth, Yuriko Hishida, Concepcion Rodriguez Esteban, Kensaku Shojima, Ling Huang, Maxim Shokhirev, Estrella Nunez-Delicado, Josep M. Campistol, Isabel Guillen-Vicente, Elena Rodriguez-Inigo, Juan Manuel Lopez-Alcorocho, Marta Guillen-Vicente, George Church, Pradeep Reddy, Pedro Guillen-Garcia, Guang-Hui Liu, Juan Carlos Izpisua Belmonte
Article
Cell Biology
Pradeep Reddy, Yanjiao Shao, Reyna Hernandez-Benitez, Estrella Nunez Delicado, Juan Carlos Izpisua Belmonte
Article
Multidisciplinary Sciences
Gerardo Ramos-Mandujano, Rahul Salunke, Sara Mfarrej, Andri Taruna Rachmadi, Sharif Hala, Jinna Xu, Fadwa S. Alofi, Asim Khogeer, Anwar M. Hashem, Naif A. M. Almontashiri, Afrah Alsomali, Digambar B. Shinde, Samir Hamdan, Pei-Ying Hong, Arnab Pain, Mo Li
Summary: The MAVRICS method, an open-source viral RNA isolation technique, is safe, fast, and scalable, enabling the detection and monitoring of pathogens with reduced biohazard risk and simplified processing procedures.
Article
Biochemistry & Molecular Biology
Tao Tan, Jun Wu, Chenyang Si, Shaoxing Dai, Youyue Zhang, Nianqin Sun, E. Zhang, Honglian Shao, Wei Si, Pengpeng Yang, Hong Wang, Zhenzhen Chen, Ran Zhu, Yu Kang, Reyna Hernandez-Benitez, Llanos Martinez Martinez, Estrella Nunez Delicado, W. Travis Berggren, May Schwarz, Zongyong Ai, Tianqing Li, Concepcion Rodriguez Esteban, Weizhi Ji, Yuyu Niu, Juan Carlos Izpisua Belmonte
Summary: This study demonstrates that human extended pluripotent stem cells have chimeric competency in cynomolgus monkey embryos, surviving, proliferating, and generating various early post-implantation cell lineages. The research also reveals signaling events underlying interspecific crosstalk, aiding in understanding the developmental trajectories of human and monkey cells within chimeric embryos.
Article
Cell Biology
Sensen Zhang, Baolei Yuan, Jordy Homing Lam, Jun Zhou, Xuan Zhou, Gerardo Ramos-Mandujano, Xueyuan Tian, Yang Liu, Renmin Han, Yu Li, Xin Gao, Mo Li, Maojun Yang
Summary: The expression of PANX1 is upregulated by various stimuli in human macrophages that promote pyroptosis, with structural and functional studies revealing its potential role in pyroptosis during immune responses. Cryo-electron microscopy and molecular dynamics simulations support the model of hPANX1 as a homo-heptamer with N-termini and C-termini extending deep into the channel pore funnel, providing insights into the channel gating mechanism.
Article
Chemistry, Multidisciplinary
Masateru Takahashi, Muhammad Tehseen, Rahul Salunke, Etsuko Takahashi, Sara Mfarrej, Mohamed A. Sobhy, Fatimah S. Alhamlan, Sharif Hala, Gerardo Ramos-Mandujano, Ahmed A. Al-Qahtani, Fadwa S. Alofi, Afrah Alsomali, Anwar M. Hashem, Asim Khogeer, Naif A. M. Almontashiri, Jae Man Lee, Hiroaki Mon, Kosuke Sakashita, Mo Li, Takahiro Kusakabe, Arnab Pain, Samir M. Hamdan
Summary: One-step reverse-transcription quantitative polymerase chain reaction (qRT-PCR) is widely used for COVID-19 diagnostics, but high cost and availability issues have led to the development of a local kit with tailored enzymes. This new kit showed robust performance in detecting COVID-19 in clinical samples and laboratory settings.
Editorial Material
Engineering, Biomedical
Shannon G. Klein, Samhan M. Alsolami, Alexandra Steckbauer, Silvia Arossa, Anieka J. Parry, Gerardo Ramos Mandujano, Khaled Alsayegh, Juan Carlos Izpisua Belmonte, Mo Li, Carlos M. Duarte
Summary: Environmental conditions in mammalian cell culture are often underreported and lack monitoring or control in biomedical studies, highlighting the urgent need for best-practice standards.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
Cell Biology
Zunpeng Liu, Wei Li, Lingling Geng, Liang Sun, Qiaoran Wang, Yang Yu, Pengze Yan, Chuqian Liang, Jie Ren, Moshi Song, Qian Zhao, Jinghui Lei, Yusheng Cai, Jiaming Li, Kaowen Yan, Zeming Wu, Qun Chu, Jingyi Li, Si Wang, Chunyi Li, Jing-Dong J. Han, Reyna Hernandez-Benitez, Ng Shyh-Chang, Juan Carlos Izpisua Belmonte, Weiqi Zhang, Jing Qu, Guang-Hui Liu
Summary: This study identified metabolic programs and metabolite effectors associated with regenerative capability, and suggested their potential application in metabolic interventions for tissue repair and regeneration.
Article
Biology
Shannon G. Klein, Samhan M. Alsolami, Silvia Arossa, Gerardo Ramos-Mandujano, Anieka J. Parry, Alexandra Steckbauer, Carlos M. Duarte, Mo Li
Summary: Mammalian cell cultures are crucial in biomedical research, but reproducibility of published experiments is often challenging. This study reveals the environmental instability of standard cell cultures, which may have widespread implications for experimental findings. Frequent monitoring of human cell lines shows significant deviations in environmental parameters during in vitro batch culture. These findings highlight the importance of improving the relevance of experimental results to in vivo physiology and enhancing reproducibility.
COMMUNICATIONS BIOLOGY
(2022)
Article
Cell Biology
Tomoaki Hishida, Mako Yamamoto, Yuriko Hishida-Nozaki, Changwei Shao, Ling Huang, Chao Wang, Kensaku Shojima, Yuan Xue, Yuqing Hang, Maxim Shokhirev, Sebastian Memczak, Sanjeeb Kumar Sahu, Fumiyuki Hatanaka, Ruben Rabadan Ros, Matthew B. Maxwell, Jasmine Chavez, Yanjiao Shao, Hsin-Kai Liao, Paloma Martinez-Redondo, Isabel Guillen-Guillen, Reyna Hernandez-Benitez, Concepcion Rodriguez Esteban, Jing Qu, Michael C. Holmes, Fei Yi, Raymond D. Hickey, Pedro Guillen Garcia, Estrella Nunez Delicado, Antoni Castells, Josep M. Campistol, Yang Yu, Diana C. Hargreaves, Akihiro Asai, Pradeep Reddy, Guang-Hui Liu, Juan Carlos Izpisua Belmonte
Summary: Mammals have limited regenerative capacity, but inducing specific factor expression can enhance liver regeneration. This study provides a new avenue for tissue regeneration.
Article
Multidisciplinary Sciences
Baolei Yuan, Xuan Zhou, Keiichiro Suzuki, Gerardo Ramos-Mandujano, Mengge Wang, Muhammad Tehseen, Lorena Cortes-Medina, James J. Moresco, Sarah Dunn, Reyna Hernandez-Benitez, Tomoaki Hishida, Na Young Kim, Manal M. Andijani, Chongwei Bi, Manching Ku, Yuta Takahashi, Jinna Xu, Jinsong Qiu, Ling Huang, Christopher Benner, Emi Aizawa, Jing Qu, Guang-Hui Liu, Zhongwei Li, Fei Yi, Yanal Ghosheh, Changwei Shao, Maxim Shokhirev, Patrizia Comoli, Francesco Frassoni, John R. Yates, Xiang-Dong Fu, Concepcion Rodriguez Esteban, Samir Hamdan, Mo Li, Juan Carlos Izpisua Belmonte
Summary: In this study, the authors revealed that WASP deficiency results in aberrant RNA splicing and that WASP regulates the transcription of splicing factor genes and co-transcriptional RNA splicing through a phase-separation process involving splicing factors and nascent RNA.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Chongwei Bi, Gerardo Ramos-Mandujano, Yeteng Tian, Sharif Hala, Jinna Xu, Sara Mfarrej, Concepcion Rodriguez Esteban, Estrella Nunez Delicado, Fadwa S. Alofi, Asim Khogeer, Anwar M. Hashem, Naif A. M. Almontashiri, Arnab Pain, Juan Carlos Izpisua Belmonte, Mo Li
Summary: NIRVANA is a method for multiplex isothermal amplification-based sequencing and real-time analysis of multiple viral genomes, capable of detecting SARS-CoV-2, influenza A, and other viruses, while monitoring mutations of SARS-CoV-2 in real time. The approach has shown high sensitivity and specificity in clinical samples and wastewater, offering a promising solution for rapid detection and mutational surveillance of pandemic viruses.