Article
Biochemistry & Molecular Biology
Hosni Cherif, Matthew Mannarino, Alain Sarabia Pacis, Jiannis Ragoussis, Oded Rabau, Jean A. Ouellet, Lisbet Haglund
Summary: In this study, single-cell transcriptomic analysis was used to identify new biomarkers for nucleus pulposus (NP) and inner annulus fibrosis (iAF) cells in intervertebral discs (IVD). The analysis also provided insights into the cell populations within non-degenerating and degenerating IVD. The findings suggest potential functional differences linked to degeneration and among NP and iAF subpopulations, and also propose specific cell type and predictive IVD degeneration genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Sebastian Wangler, Amir Kamali, Christina Wapp, Karin Wuertz-Kozak, Sonja Haeckel, Claudia Fortes, Lorin M. Benneker, Lisbet Haglund, R. Geoff Richards, Mauro Alini, Marianna Peroglio, Sibylle Grad
Summary: MSC’s secretome composition was analyzed after exposure to different IVD environments, showing more complex secretome induced by IVD conditioned media compared to IL-1 beta stimulation. The secretome reflected different activity based on IVD pathological status, with implications for tailored secretome therapy development.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell Biology
Zhikun Wang, Yangming Wu, Zhonghan Zhao, Chengyi Liu, Lingli Zhang
Summary: Exosomes are lipid vesicles containing various bioactive substances that may improve bone microenvironment and resist bone metastasis. BMSC-Exos offer new ideas for disease prevention and rehabilitation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Materials Science, Biomaterials
Ziyan Wang, Feixue Mi, Jinchen Li, Dandan Chen, Minmin Lin, Xinyu Wang, Siying Wu, Changfeng Wu, Chao Liu
Summary: Bone marrow stromal cells (BMSCs) have great potential in bone defect repair. By using semiconducting polymer dots (Pdots) as markers, we successfully selected BMSCs and evaluated their therapeutic efficacy in a bone defect model.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Matthew Mannarino, Oliver Wu-Martinez, Kai Sheng, Li Li, Rodrigo Navarro-Ramirez, Peter Jarzem, Jean A. Ouellet, Hosni Cherif, Lisbet Haglund
Summary: This study aimed to determine if combining o-Vanillin and RG-7112 could more efficiently remove senescent cells and reduce inflammatory factors and pain mediators in degenerating intervertebral disc (IVD) cells. The results showed that the combination of these two senolytic drugs significantly reduced the amount of senescent IVD cells, proinflammatory cytokines, and neurotrophic factors. These findings support the potential of senolytics as a promising treatment for IVD-related low back pain.
Article
Immunology
Raghavan Chinnadurai, Paul D. Bates, Keith A. Kunugi, Kwangok P. Nickel, Larry A. DeWerd, Christian M. Capitini, Jacques Galipeau, Randall J. Kimple
Summary: MSCs are being tested as a cell therapy in clinical trials for inflammatory disorders, but suitable preclinical animal models are rare. In this study, two robust animal models of immune pathology were introduced to study the effect of IFN gamma primed bone marrow derived MSCs in acute radiation syndrome and graft versus host disease. The results showed that infused IFN gamma primed allogeneic MSCs protect against lethality from ARS, but not GvHD.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, General & Internal
Daisuke Ukeba, Katsuhisa Yamada, Takashi Suyama, Darren R. Lebl, Takeru Tsujimoto, Takayuki Nonoyama, Hirokazu Sugino, Norimasa Iwasaki, Masatoki Watanabe, Yumi Matsuzaki, Hideki Sudo
Summary: This is an open access article published by Elsevier B.V. The authors hold the copyright and it is available under the CC BY license, with support for superscript and subscript.
Article
Cell & Tissue Engineering
Xiao Yang, Zhiqian Chen, Chen Chen, Chen Han, Yifan Zhou, Xunlin Li, Haijun Tian, Xiaofei Cheng, Kai Zhang, An Qin, Tangjun Zhou, Jie Zhao
Summary: The study demonstrated that patients with intervertebral disc degeneration had increased levels of fibrotic markers in their tissues. Bleomycin induced a pro-fibrotic effect on annulus fibrosus cells and bone marrow stromal cells through the TGF beta-TGF beta R1-SMAD2/3 signaling pathway. Pharmacological inhibition or gene knock-down of TGF beta R1 could mitigate the pro-fibrotic effects.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Martina Pesaresi, Sergi A. Bonilla-Pons, Ruben Sebastian-Perez, Umberto Di Vicino, Marc Alcoverro-Bertran, Ralph Michael, Maria Pia Cosma
Summary: This study demonstrates that chemokines released from degenerating retinae can drive the migration of transplanted stem cells, and overexpression of chemokine receptors can improve the effectiveness of stem cell therapy by enhancing rescue of cell death and preserving electrophysiological function.
Review
Biochemistry & Molecular Biology
Andreas S. Croft, Svenja Illien-Junger, Sibylle Grad, Julien Guerrero, Sebastian Wangler, Benjamin Gantenbein
Summary: The use of MSCs for IVD regeneration has shown significant potential in recent years, with efforts focused on utilizing their homing capabilities to repair damaged or degenerated IVDs. This review provides an overview of achievements in both preclinical and clinical applications, as well as current limitations and future perspectives in utilizing MSCs for IVD regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Matthias Jung, Susanne Rospleszcz, Maximilian T. Loeffler, Sven S. Walter, Elke Maurer, Pia M. Jungmann, Annette Peters, Johanna Nattenmueller, Christopher L. Schlett, Fabian Bamberg, Lena S. Kiefer, Thierno D. Diallo
Summary: This study assessed the association between lumbar bone marrow adipose tissue fat fraction (BMAT-FF) and paraspinal muscle proton density fat fraction (PDFF) with intervertebral disc degeneration (IVDD). The results showed a significant correlation between BMAT-FF and PDFF, and both factors were found to be independent and additive risk factors for IVDD. The findings suggest that quantitative MRI measurements of paraspinal myosteatosis and vertebral bone marrow fatty infiltration can serve as imaging biomarkers to assess the individual risk for IVDD.
EUROPEAN RADIOLOGY
(2023)
Article
Biology
Zheting Liao, Yu Jin, Yuhao Chu, Hansen Wu, Xiaoyu Li, Zhonghao Deng, Shuhao Feng, Nachun Chen, Ziheng Luo, Xiaoyong Zheng, Liangxiao Bao, Yongqing Xu, Hongbo Tan, Liang Zhao
Summary: A study using single-cell RNA sequencing explored the transcriptomic landscape of alcohol-induced osteonecrosis of the femoral head (ONFH). The study revealed the relevance of stromal and endothelial cells to the pathogenesis of the disease and provided resources for developing cell type-specific therapeutic strategies.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Andrei Mircea Vacaru, Ana-Maria Mazilu, Madalina Dumitrescu, Ioana Madalina Fenyo, Anca Violeta Gafencu, Ana-Maria Vacaru
Summary: This study found that treatment with FasL-overexpressing MSC can improve GvHD and increase survival in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Gunawan Widjaja, Abduladheem Turki Jalil, Hendrik Setia Budi, Walid Kamal Abdelbasset, Syahril Efendi, Wanich Suksatan, Rauza Sukma Rita, Andri Praja Satria, Surendar Aravindhan, Marwan Mahmood Saleh, Mohammed Nader Shalaby, Alexei Valerievich Yumashev
Summary: The application of mesenchymal stromal/stem cells (MSCs) and their exosomes in treating degenerative diseases, especially intervertebral disc disease (IDD), shows promising results. MSCs exert therapeutic effects by differentiating, reducing apoptosis, increasing proliferation, and decreasing senescence in nucleus pulposus cells (NPCs). The application of MSCs and their exosomes also affects annulus fibrosus and cartilaginous endplate cells, preventing the progression of disc degeneration.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Cell & Tissue Engineering
Wentao Zhang, Tianze Sun, Ying Li, Ming Yang, Yantao Zhao, Jing Liu, Zhonghai Li
Summary: Stem cell therapy for intervertebral disc degeneration is emerging as a potential treatment option, with evidence from in vitro, in vivo, and clinical trials showing its effectiveness in relieving pain and symptoms, reversing the degenerative cascade, delaying the aging process, and maintaining spinal function. Further research is needed to understand the mechanisms and effects of stem cells in IDD treatment.
STEM CELL RESEARCH & THERAPY
(2022)
