Journal
SOUTH AFRICAN JOURNAL OF BOTANY
Volume 77, Issue 1, Pages 160-169Publisher
ELSEVIER
DOI: 10.1016/j.sajb.2010.07.010
Keywords
Actinidia deliciosa; Agrobacterium tumefaciens; AtNHX1; Salt tolerance
Categories
Funding
- National Natural Science Foundation of China [30870194]
- Research Project of Provincial Key Laboratory of Shaanxi [08JZ70]
- Development Project of Science and Technology Research of Shaanxi Province [2010 K16-04-01]
- Postgraduate Innovation Research Project of Northwest University [09YZZ58, 09YSY38]
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Salinity is the main limiting factor of plant growth and agricultural productivity. A lot of previous works showed that the introduction of Na+/H+ antiporter gene could improve the tolerance of plants to salt. In this study, a vacuolar Na+/H+ antiporter gene, AtNHX1 from Arobidopsis, was transferred into kiwifruit by Agrobacterium-mediated protocol. Polymerase chain reaction (PCR) and Southern blot analysis confirmed that AtNHX1 was successfully integrated into the kiwifruit genome. Reverse transcription (RT)-PCR analysis indicated that AtNHX1 expressed highly in transgenic plants. It was found that transgenic kiwifruit plants exhibited improved resistance to 200 mmol/l NaCl in comparison with wide-type plants. Under salt stress, these transgenic lines accumulated more Na+ than control, due to an increased Na+/H+ antiporter activity. In physiological analysis, the traits such as osmotic adjustment and antioxidation capability of transgenic lines under salt stress were obviously higher than that of wide-type plants. These results suggested that the overexpression of vacuolar Na+/H+ antiporter gene could increase the salt tolerance of kiwifruit. (C) 2010 SAAB. Published by Elsevier B.V. All rights reserved.
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