Journal
SOUTH AFRICAN JOURNAL OF BOTANY
Volume 76, Issue 3, Pages 511-516Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.sajb.2010.03.009
Keywords
2,4-D; Anther culture; Cyanobacteria; Maize; Microalgae
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This is the first report on the beneficial effect of microalgal and cyanobacterial biomass on anther cultures of maize (Zea mays L.). Investigations were made on the cytokinin- and auxin-like activity and content of terrestrial and fresh-water living microalgal and cyanobacterial strains. The influence of media supplemented with biomass from four selected strains on the anther induction, the frequency of microspore-derived embryo-like structures, and regeneration capacity in anther cultures of maize was also studied. The addition of cyanobacterial and microalgal biomass to the induction and regeneration media in concentrations of 1 or 2 g/L improved the androgenic response, and was able to reduce the quantity of the synthetic auxin 2,4-dichlorophenoxy-acetic acid (2,4-D) required, or replace it completely. (C) 2010 SAAB. Published by Elsevier B.V. All rights reserved.
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