4.3 Article

The analysis of magnesium transport system from external solution to xylem in rice root

Journal

SOIL SCIENCE AND PLANT NUTRITION
Volume 57, Issue 2, Pages 265-271

Publisher

TAYLOR & FRANCIS LTD
DOI: 10.1080/00380768.2011.576397

Keywords

isotherm; kinetic; magnesium; rice; transport

Funding

  1. Grants-in-Aid for Scientific Research [10J04154] Funding Source: KAKEN

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We report the characterization of magnesium (Mg) transport from external solution to xylem by using xylem fluid analysis in rice (Oryza sativa L. Nipponbare). It was indicated that Mg uptake and/or xylem loading was mediated by two transport systems, a saturable and a linear component. The Km value of the saturable component was approximately 20 mu M, and the saturated concentration was calculated to be 1.5 mM. Using a multi-compartment transport box with excised roots, the transfer from external solution to xylem was shown to be inhibited by hexaamminecobalt(III) {[Co(III)(NH3)6], Co-Hex}, a selective inhibitor of CorA-mediated influx of Mg2+, suggesting that the MRS2/MGT genes that belong to the superfamily of CorA-type membrane proteins are involved in the Mg transport system.

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