Article
Biochemistry & Molecular Biology
Junhao Zhuge, Xiang Zhou, Lifeng Zhou, Jiafu Hu, Kai Guo
Summary: This study developed the Plant Parasitic Nematodes Database (PPND), a platform that provides genomic, transcriptomic, protein, and functional annotation data for plant parasitic nematode research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Entomology
Hai Yan Wu, Juliana de Oliveira Silva, Jennifer Smith Becker, J. Ole Becker
Summary: Plant-parasitic nematodes are significant pests for many crops worldwide. Fluazaindolizine, a fluorinated nematicide, shows strong toxic activity against certain nematode species with low mammalian toxicity. The compound impacts nematode parasitism at non-lethal concentrations and short-term exposure.
JOURNAL OF PEST SCIENCE
(2021)
Article
Biology
Zhanar Tileubayeva, Aleksei Avdeenko, Svetlana Avdeenko, Natalia Stroiteleva, Sergey Kondrashev
Summary: This study investigated plant-parasitic nematodes affecting greenhouse vegetables, finding Meloidogyne, Helicotylenchus, and Pratylenchus to be the most common genera, while onions were not infected. The results indicate that soil properties are as important as host plants for the abundance, distribution, and structure of nematode communities, potentially aiding in improving vegetable pest control.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Plant Sciences
Alessandro Cabianca, Laurin Mueller, Katharina Pawlowski, Paul Dahlin
Summary: The study found that changes in sterols in important crops after infection with Meloidogyne incognita mainly manifest as a change in the beta-sitosterol/stigmasterol ratio, particularly an increase in beta-sitosterol and a decrease in stigmasterol in tomato, soybean, cucumber, and corn. Cholesterol levels increased in tomato, cucumber, and corn, while being often undetectable in uninfected plants. The expression of the sterol 22C-desaturase gene CYP710A11 in tomato was found to be consistent with the sterol profile after M. incognita infection, providing new insights into plant-nematode interactions.
Article
Cell Biology
Rahim Hassanaly-Goulamhoussen, Ronaldo De Carvalho Augusto, Nathalie Marteu-Garello, Arthur Pere, Bruno Favery, Martine Da Rocha, Etienne G. J. Danchin, Pierre Abad, Christoph Grunau, Laetitia Perfus-Barbeoch
Summary: The root-knot nematode Meloidogyne incognita adapts rapidly to unfavorable conditions through epigenetic mechanisms, impacting gene expression and sharing similar epigenetic signatures with the model nematode Caenorhabditis elegans. The H3K4me3 histone modification is strongly correlated with high expression of genes during specific developmental stages, providing insights into the dynamic regulation of parasitism genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Savina R. Cammalleri, Jessica Knox, Peter J. Roy
Summary: This article discusses the damage caused by plant-parasitic nematodes and demonstrates how to culture and collect these nematodes for screening novel nematicides, addressing a significant issue in global food production.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Plant Sciences
Pasqua Veronico, Nicola Sasanelli, Alberto Troccoli, Arben Myrta, Audun Midthassel, Tariq Butt
Summary: Fungal volatile organic compounds (VOCs), particularly 1-Octen-3-ol, are highly toxic to root-knot nematodes and show potential as ecofriendly alternatives to synthetic nematicides. Exposure to 1-Octen-3-ol and 3-Octanone resulted in high mortality rates of the nematodes, with 1-Octen-3-ol exhibiting greater nematicidal activity than 3-Octanone.
Article
Microbiology
Maikel S. S. Hislop, Orchid M. M. Allicock, Darani A. A. Thammavongsa, Sidiya Mbodj, Allison Nelson, Albert C. C. Shaw, Daniel M. M. Weinberger, Anne L. L. Wyllie
Summary: Testing saliva samples with quantitative PCR (qPCR) improves the sensitivity of detection of pneumococcal carriage, however, concerns have been raised regarding the specificity of this approach. The widely used lytA qPCR assay may generate false positive results and nonpneumococcal Streptococcus species are responsible for this signal. It is important to test for the presence of multiple gene targets for reliable and specific detection of pneumococcus in respiratory tract samples.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biodiversity Conservation
Nadine Ali, Johannes Tavoillot, Bernard Martiny, Odile Fossati-Gaschignard, Stephan Plas, Ewa Dmowska, Grazyna Winiszewska, Elodie Chapuis, Elsa Rulliat, David Jimenez, Gilles Granereau, Stephane Barbier, Cecile Heraud, Nadine Gautheron, Veronique Edel-Hermann, Christian Steinberg, Thierry Mateille
Summary: This study compares the diversity and community patterns of soil-borne parasites in different successive ecosystems, revealing significant differences in community structures. Sediment legacy and soil hydromorphicity are identified as important factors influencing these differences.
GLOBAL ECOLOGY AND CONSERVATION
(2021)
Article
Plant Sciences
Min Wang, Guicai Du, Junna Fang, Linsong Wang, Qunqun Guo, Tingting Zhang, Ronggui Li
Summary: Pine wilt disease (PWD) caused by Bursaphelenchus xylophilus is a major threat to pine forests. The UGT440A1 gene was found to be expressed in all developmental stages of B. xylophilus and plays a role in the pathogenic process of PWD.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Microbiology
Anita Bollmann-Giolai, Jacob G. Malone, Sanu Arora
Summary: The relationship between plants and fungi has significant impacts on plant health and soil ecosystems. Fungal community diversity can enhance plant disease resistance and improve rhizosphere soil health. In addition, researchers have developed various diagnostic tools for detecting fungal pathogens and analyzing rhizosphere communities.
CURRENT OPINION IN MICROBIOLOGY
(2022)
Article
Agronomy
Lucas Ripa, G. N. Stevens, E. E. Lewis
Summary: Plants can act as mediators to connect belowground and aboveground heterotrophic communities. This study aimed to determine how the activity of one herbivore affects the acceptability of a plant to another herbivore. The results showed that plants can mediate interactions between belowground and aboveground herbivores.
Article
Plant Sciences
Aboubakr Moradi, Mohamed El-Shetehy, Jordi Gamir, Tina Austerlitz, Paul Dahlin, Krzysztof Wieczorek, Markus Kunzler, Felix Mauch
Summary: The study showed that expressing Coprinopsis cinerea lectin 2 (CCL2) in Arabidopsis enhanced plant resistance against various nematodes and fungal pathogens, while also improving plant growth and biomass production. The mechanism of CCL2-mediated disease resistance enhancement depended on its fucoside-binding ability, and not on direct growth inhibition. Transcriptional induction of defense genes was significantly increased in CCL2-expressing plants compared to wild type plants, indicating the potential of fungal defense lectins in plant protection.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Magdalena Skotniczny, Pawel Satora
Summary: Lactiplantibacillus plantarum is a widely distributed lactic acid bacterium with a large and flexible genome, resulting in high strain diversity. This review provides an overview of molecular techniques, both culture-dependent and culture-independent, used for the detection and identification of L. plantarum. Some of these techniques can also be applied to the analysis of other lactic acid bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Ekaterina A. Goncharova, Vladimir G. Dedkov, Anna S. Dolgova, Ilia S. Kassirov, Marina V. Safonova, Yana Voytsekhovskaya, Areg A. Totolian
Summary: The study developed a new RT-qPCR assay for rapid and accurate detection of COVID-19. The assay showed high sensitivity and specificity, making it suitable for various hospitals and laboratories.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Agronomy
Motonori Takagi, Maki Goto, David Wari, Mina Saito, Roland N. Perry, Koki Toyota
Article
Agronomy
Masanori Kawanobe, Soh Sugihara, Naoko Miyamaru, Koichi Yoshida, Eito Nonomura, Hiroaki Oshiro, Koki Toyota
Article
Zoology
Sinh Van Nguyen, Phuong Kim Thi Nguyen, Masaaki Araki, Roland N. Perry, Linh Ba Tran, Khoi Minh Chau, Yu Yu Min, Koki Toyota
Summary: The study investigated the seasonal variation and spatial distribution of nematode community in rice cultivation areas in Vietnam, and found that the abundance of Hirschmanniella parasitic nematodes in soil was significantly negatively correlated with rice yield.
Article
Zoology
Seiya Chikamatsu, Ai Takeda, Kazuhiro Ohta, Takeo Imura, Roland N. Perry, Koki Toyota
Summary: Short-term cultivation of mung bean and ploughing can effectively reduce the density of soybean cyst nematode in soil and increase the yield of green soybean. Results showed that the best outcomes were achieved at temperatures between 25-30 degrees Celsius and soil moisture levels below pF 2.8.
Article
Plant Sciences
Koki Toyota
Summary: Continuous cropping can cause yield losses by damaging soil biological and chemical properties, but organic matter amendment is important for managing soilborne plant pathogens and nematodes. Examples of organic matter amendment include composts, cover crops, and simple organic compounds to suppress pathogen and nematode densities. A novel diagnostic method using real-time PCR is also shown for plant parasitic nematodes in this review.
SOIL SCIENCE AND PLANT NUTRITION
(2021)
Article
Biodiversity Conservation
Van Sinh Nguyen, Minh Khoi Chau, Quang Minh Vo, Van Khoa Le, Thi Kim Phuong Nguyen, Masaaki Araki, Roland N. Perry, Anh Duc Tran, Duy Minh Dang, Ba Linh Tran, Gyu Lee Chol, Koki Toyota
Summary: Saltwater intrusion reduces the abundance of nematodes in soil, with differences in impacts between alluvial and acid sulfate soils. Omnivorous nematodes were found more in nonsalt-affected alluvial soil, while certain genera like Mesodorylaimus were dominant in salt-affected acid sulfate soil. Bacterivorous nematodes were dominant in both non-salt affected and salt-affected alluvial soils, but less prevalent in salt-affected acid sulfate soil, indicating tolerance differences to salinity levels.
ECOLOGICAL INDICATORS
(2021)
Article
Agronomy
Nguyen Van Sinh, Chau Minh Khoi, Nguyen Thi Kim Phuong, Tran Ba Linh, Dang Duy Minh, Roland N. Perry, Koki Toyota
Summary: Reducing intensive rice cultivation can decrease plant-parasitic nematodes and increase bacterivorous nematodes in salt-affected soils. Mixing reduced IRC with compost or silicate fertilizer can enhance nematode community structure, biodiversity, and trophic structure in the soil. This study highlights the potential benefits of these practices in improving soil health and nematode diversity.
Article
Soil Science
Kozue Sawada, Yoshiyuki Inagaki, Koki Toyota
Summary: This study revealed that increasing the turnover of pre-existing biomass N can lead to a positive priming effect, accelerating net soil C loss.
APPLIED SOIL ECOLOGY
(2021)
Article
Soil Science
Kozue Sawada, Yoshiyuki Inagaki, Soh Sugihara, Shinya Funakawa, Karl Ritz, Koki Toyota
Summary: The conversion of natural forests into plantations has a significant impact on soil properties and microbial diversity. Different tree stand types and dominant species can influence the community structures of bacteria and fungi, resulting in changes in diversity and abundance. Specific bacteria with plant-growth-promoting properties were found to be reduced in pure cedar plantations compared to mixed natural forests, highlighting the importance of considering ecosystem diversity when planning forestry practices.
APPLIED SOIL ECOLOGY
(2021)
Article
Soil Science
Masanori Kawanobe, Koki Toyota, Karl Ritz
Summary: This study developed highly sensitive primer sets targeting the 18S rDNA region for soil nematode fauna using NGS technology. The NGS analysis of soil DNA with these primer sets showed a higher diversity and more nematode species detection compared to traditional morphological methods. The results indicate that the developed primer sets and metabarcoding protocol are suitable for comprehensive studies of nematode community structure.
APPLIED SOIL ECOLOGY
(2021)
Article
Microbiology
Kozue Sawada, Shinichi Watanabe, Ho Lam Nguyen, Soh Sugihara, Mayuko Seki, Hana Kobayashi, Koki Toyota, Shinya Funakawa
Summary: Deforestation of native tropical forests has led to the expansion of fast-growing tree plantations like Acacia spp. This study in Vietnam found that soil pH may be a critical factor affecting bacterial diversity, and Acacia tree roots supported more copiotrophic bacteria and plant pathogenic fungi compared to native tree roots. Such changes in fungal community structures could impact the sustainable productivity of acacia plantations in the future.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agronomy
Nguyen Van Sinh, Risako Kato, Doan Thi Truc Linh, Nguyen Thi Kim Phuong, Koki Toyota
Summary: Biochar has the potential to improve soil properties and ecological functions, but it can also negatively impact soil organisms. This study found that the application of biochar at different rates and types had significant effects on the composition and abundance of soil nematode communities.
Article
Agronomy
Doan Thi Truc Linh, Chau Minh Khoi, Karl Ritz, Nguyen Van Sinh, Nguyen Thi Kim Phuong, Huynh Mach Tra My, Tran Ba Linh, Dang Duy Minh, Thi Tu Linh, Koki Toyota
Summary: The objective of this study was to investigate the effects of organic amendment of salinized acid soils on P fractions, enzyme activities, and associated rice yield. The results showed that biochar increased labile and moderately labile P forms, while compost had a relatively modest effect. There was no significant difference in rice yield between triple and double rice crops without organic amendments.
Correction
Horticulture
Atsushi Takada, Kunihiko Okada, Koki Toyota
HORTICULTURE JOURNAL
(2021)
Article
Horticulture
Atsushi Takada, Kunihiko Okada, Koki Toyota
Summary: Spring cabbage is a crop type with sowing in fall and harvesting in spring. Chilling induces flower bud differentiation from vegetative to reproductive phase, triggering the risk of premature bolting under long days and high temperatures. The study proposes a model to predict flower bud differentiation and head leaves using daily cumulative temperature data.
HORTICULTURE JOURNAL
(2021)