Journal
SOIL BIOLOGY & BIOCHEMISTRY
Volume 70, Issue -, Pages 12-21Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.soilbio.2013.12.002
Keywords
Stable isotope probing; Maize; C-13; Decomposition; Denitrification; Nitrogen fixation; nifH; nirS
Categories
Funding
- National Natural Science Foundation of China
- 12th Five-Year Key Programs for Science and Technology Development of China
- Institute of Agricultural Resources and Regional Planning of Chinese Academy of Agricultural Sciences
Ask authors/readers for more resources
The links between microbial taxa, in situ organic matter decomposition, and coupling of carbon (C) and nitrogen (N) cycles remain unresolved. Here, we used stable isotope probing (SIP) technique to investigate bacterial carbon assimilation and C and N coupling during decomposition of C-13-labeled maize residue in a black soil from Northeast China. Bacteria assimilating carbon from maize residue (16S rRNA analysis) were primarily distributed in the Phyla Actinobacteria, Firmicutes and Proteobacteria. These include the recognized stubble decomposing lineages of Arthrobacter, Streptomyces, Bacillus and Rhizobium, but also lineages not previously reported (Agromyces, Blastococcus, Gemmatimonas, Glycomyces, Heliobacillus, Lysobacter, Microlunatus, Myco plasma, Natronocella, Ohtaekwangia, Paenibacillus, Schlegelella, Sorangium, Steroidobacter and Thermacetogenium). Analysis of nitrogen fixation (nifH) and denitrification (nirS) genes in heavy-fraction DNA was used to link microbial taxa involved in N cycling to C transformation of the maize residue. A cluster of nifH genotypes affiliated with Rhizobium and two other 'uncultured' clusters dominated the N-fixing clone library, and genotypes affiliated with Kocuria varians and an uncultured cluster dominated the library of nitrite reducing (nirS) taxa. The results suggest that plant residue decomposition may stimulate both N-fixation and denitrification through direct C-feeding of related microbes in soil. (C) 2013 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available