Effects of organic and mineral amendments on available P and phosphatase activities in a degraded Mediterranean soil under short-term incubation experiment

The aim of this study was to determine the effects of mineral and organic-P-fertilizers on soil P availability, bacteria densities and phosphatase activities, in a degraded Mediterranean soil characterized by low level in soil organic matter and nutrients. A typical degraded Mediterranean soil, originating from a siliceous mineral parent material, was amended with different organic or mineral P-sources: aerobically digested sewage sludge (SS), with or without physico-chemical treatment by ferric chloride; sewage sludge compost (SSC); Na or K mineral P-salts (Pi-salts). All the amendments were carried out in order to provide soil with a P total quantity equivalent to 0.5 g P2O5/kg of soil. Bacterial density, phosphatase activities (i.e. acid (APH) and alkaline (BPH) phosphomonoesterases and phosphodiesterases), BPH/APH ratio, and available P (P Olsen) were measured after 25 and 87 days of incubation. Results showed that all the P-sources used to fertilize soil during this study resulted in significant increase in P concentration. However, different responses in phosphatase activities and bacterial densities were obtained with regards to the amendment applied to soil. Indeed, it appeared clearly that sewage sludge (SS) considerably stimulated soil biological activity, and more especially the different kinds of phosphatases involved in P mineralization and P turn-over. On the contrary, sewage sludge compost (SSC) as well as P-salts amendments did not affected these parameters in most cases. Results showed also that the incubation time influenced almost all the biological and chemical parameters investigated during this study. As a consequence, P availability was considerably improved in the amended soils between the two sampling dates. (c) 2007 Elsevier B.V. All rights reserved.