Article
Agriculture, Dairy & Animal Science
Natalie Rizkallah, Caitlin G. Chambers, Simon P. de Graaf, Jessica P. Rickard
Summary: The success of semen preservation is crucial for artificial reproductive technologies in sheep. Cold storage at reduced temperatures can harm the sperm, but recent studies have shown that room-temperature liquid storage may be a viable alternative. This review summarizes the factors affecting the survival of ram sperm during liquid storage and the potential role of pro-survival factors and antioxidants in mitigating the damaging effects of lipid peroxidation. This knowledge could help establish a new method of semen preservation in the sheep industry that maximizes fertility after storage and artificial insemination.
Article
Agriculture, Dairy & Animal Science
Cristina Palacin-Martinez, Mercedes Alvarez, Rafael Montes-Garrido, Marta Neila-Montero, Luis Anel-Lopez, Paulino de Paz, Luis Anel, Marta F. Riesco
Summary: Sperm cryopreservation plays a crucial role in optimizing artificial insemination protocols for rams. Previous efforts to improve ram sperm cryopreservation protocols have focused on different methods, such as using different cryoprotectants, antioxidants, or freezing-thawing rates. However, the influence of male sexual regimes on freezeability has not been studied until now. In this study, a multiparametric analysis was conducted to evaluate the quality of cooled and frozen-thawed semen after different sperm collection frequencies. The results showed that semen quality significantly decreased in the groups with zero and ten collections per week compared to the group with four collections per week. Additionally, the apoptosis rate significantly increased in the group with ten collections per week. Enzyme activity related to redox balance, such as glutathione peroxidase and superoxide dismutase, also showed significant decreases in the group with ten collections per week. This study highlights the importance of considering male sexual regimes in alternative strategies for sperm cryopreservation in order to improve frozen-thawed sperm quality.
Article
Multidisciplinary Sciences
Shereen Akhter, Muhammad Zubair, Majid Mahmood, Syed Murtaza Hassan Andrabi, Nasir Hameed, Ejaz Ahmad, Muhammad Kashif Saleemi
Summary: This study investigated the effects of vitamin C, vitamin E, and their combination on the semen quality of Kail rams. The results showed significant effects of these antioxidants on certain sperm parameters, but no significant effects on viability and plasma membrane integrity.
SCIENTIFIC REPORTS
(2023)
Article
Veterinary Sciences
Ayman A. A. Swelum, Hani A. A. Ba-Awadh, Isiaka O. O. Olarinre, Islam M. M. Saadeldin, Abdullah N. N. Alowaimer
Summary: The effects of adding mixed chicken and Japanese quail egg yolks (EYs) to the cryodiluent on the quality of ram semen before freezing and post-thawing were evaluated. The study found that the use of chicken egg yolks improved sperm vitality, plasma membrane integrity, and DNA integrity, while the use of quail egg yolks improved sperm abnormalities and kinetic motion parameters. The addition of mixed egg yolks had no additional benefits for semen parameters.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Jian-qing Zhao, Guo-liang Xiao, Wen-liang Zhu, Di Fang, Na Li, Chun-mei Han, Qing-hua Gao
Summary: The study evaluated the preservation of ram semen at 0 degrees C using soybean lecithin with a Tris-fructose extender. Results showed that the 0.5% concentration of soybean lecithin was effective in maintaining sperm motility and integrity, leading to successful artificial insemination and normal offspring production at 0 degrees C.
Article
Agriculture, Dairy & Animal Science
Payungsuk Intawicha, Tossapol Moonmanee, Sureeporn Saengwong, Chompunut Lumsangkul, Yu-Jing Liao, Wilasinee Inyawilert
Summary: This study compared the effects of fresh and lyophilized egg yolk on the quality of cryopreserved goat semen. The results showed that the addition of 20% fresh egg yolk had the best effect, but the addition of 10% lyophilized egg yolk had similar results to 20% fresh egg yolk. Therefore, 10% lyophilized egg yolk can be used as an alternative extender to fresh egg yolk for mitigating microbiological risks.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Agriculture, Dairy & Animal Science
Julio Constantino Molina, Matheus Batista de Oliveira, Paulo Henrique Conceica Costa, Edmilson Willian Propheta dos Santos, Cristiane Bani Correa, Hymerson Costa Azevedo
Summary: The study aimed to evaluate the effect of different concentrations of soy lecithin and freezing rates on semen quality after thawing. The results showed that the combination of 20% egg yolk and 2.0% soy lecithin had the best cryoprotective effects. Changes in spermatozoa after thawing were related to the type of extender, the amounts of the same compound in the extender, and the freezing rates to which they were subjected.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Veterinary Sciences
Maryam Moradi, Hadi Hajarian, Hamed Karamishabankareh, Leila Soltani, Bijan Soleymani
Summary: The study found that the use of MNPs for separating X and Y chromosome-bearing spermatozoa had no significant adverse effects on semen parameters after cryopreservation.
VETERINARY MEDICINE AND SCIENCE
(2022)
Article
Andrology
Fateme Zarei, Hossein Daghigh-Kia, Reza Masoudi
Summary: The study found that the new mitochondria-targeted antioxidant Mito-TEMPO (5 and 50 μM) significantly improved post-thawed quality parameters of sheep sperm, including motility parameters, average path velocity, membrane functionality, mitochondria activity, and viability. Additionally, it reduced apoptotic-like changes, lipid peroxidation, and ROS concentration.
Article
Multidisciplinary Sciences
Frankie Tu, Maajid Bhat, Patrick Blondin, Patrick Vincent, Mohsen Sharafi, James D. Benson
Summary: This study demonstrates the optimization of bull sperm cryopreservation media using supervised machine learning, resulting in significantly improved post-thaw motility. The research provides a template for optimization in other cell types.
SCIENTIFIC REPORTS
(2022)
Article
Veterinary Sciences
Hani A. Ba-Awadh, Isiaka O. Olarinre, Abdullah N. Alowaimer, Islam M. Saadeldin, Ayman A. Swelum
Summary: This study aimed to compare two freezing protocols for Najdi ram's semen. The rapid freezing protocol showed better post-thawing semen quality compared to the slow freezing protocol, including higher vitality, fast progressive motility, straight-line velocity, average pathway velocity, linearity, and wobble.
SLOVENIAN VETERINARY RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
James D. Gillis, Linda M. Penfold, Natalie D. Mylniczenko
Summary: This study explored the reproductive biology and sperm cryopreservation of southern stingrays, finding correlations between plasma testosterone levels and semen quality, as well as the potential benefits of a slow freezing method in improving post-thaw sperm survival.
Article
Agriculture, Dairy & Animal Science
Jakub Vozaf, Andrea Svoradova, Andrej Balazi, Jaromir Vasicek, Lucia Olexikova, Linda Dujickova, Alexander Makarevich, Rastislav Jurcik, Hana Duranova, Peter Chrenek
Summary: Biodiversity protection is a vital challenge, and cryopreservation of sperm is an effective tool for animal genetic resource conservation. This study compares the quality parameters of cryopreserved sperm among three different Slovak sheep breeds. The study finds that the selected cryopreservation protocol is suitable for all three breeds and can greatly benefit biodiversity protection.
Article
Cell Biology
Asmaa E. Sharaf, Wael A. Khalil, Ezz Khalifa, Mohamed A. Nassan, Ayman A. Swelum, Mostafa A. El-Harairy
Summary: This study examined the effects of supplementing bee bread extract (BBE) in extenders on the quality of cryopreserved ram semen. The results showed that the supplementation of BBE improved sperm motility, viability, and membrane integrity. BBE also promoted antioxidant and enzymatic activities and reduced apoptosis in semen during freezing.
Article
Agriculture, Dairy & Animal Science
Margarida Fernandes, Pablo Rodriguez Hernandez, Joao Simoes, Joao Pedro Barbas
Summary: The study revealed that the egg yolk-based semen extender performed better in cryopreserving semen from Merino rams. However, there were significant interactions between different semen extenders, semen processing, and collection months on sperm parameters. Further research is needed to address the causes of these interaction effects and improve semen extenders.
Article
Veterinary Sciences
Calogero Stelletta, Koray Tekin, Beste Cil, Fatma Oztutar Stelletta, Ali Daskin
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
(2018)
Article
Agriculture, Dairy & Animal Science
M. B. Tirpan, K. Tekin, B. Cil, H. Alemdar, M. E. Inanc, K. T. Olgac, C. Stelletta, A. Daskin
Article
Biology
Koray Tekin, Ali Daskin
Article
Veterinary Sciences
Mehmet Akkose, Cigdem Cebi Sen, Adnan Kirmit, Omer Korkmaz, Murat Onur Yazlik, Murat Gocen, Mushap Kuru, Ergun Akcay
VETERINARIA MEXICO
(2019)
Article
Biotechnology & Applied Microbiology
Begum Yurdakok-Dikmen, Calogero Stelletta, Koray Tekin, Ozgur Kuzukiran, Ali Daskin, Ayhan Filazi
Article
Andrology
Koray Tekin, Beste Cil, Havva Alemdar, Kemal Tuna Olgac, Mehmet Borga Tirpan, Ali Daskin, Calogero Stelletta
Article
Biotechnology & Applied Microbiology
Koray Tekin, Pinar Arslan, Beste Cil, Ayhan Filazi, Ergun Akcay, Begum Yurdakok-Dikmen
Article
Andrology
Ilktan Bastan, Ergun Akcay
Summary: In this study, the quality of frozen bull semen was evaluated using the proAKAP4 level test. The results showed that there is a positive correlation between proAKAP4 level and sperm motility parameters, while a negative correlation was found between proAKAP4 level and abnormal sperm tail percentage. Samples with proAKAP4 concentrations of 25 ng/10(6) spermatozoa and higher preserved certain motility characteristics. Overall, proAKAP4 has the potential to be a biomarker for assessing the quality of frozen-thawed semen.
Article
Biology
Kemal Tuna Olgac, Ergun Akcay
Summary: This study evaluated the effects of polyamines on stallion semen parameters, finding that the addition of 1-2 mg/ml Spermine can increase cryopreservation success without damaging other sperm properties.
Meeting Abstract
Agriculture, Dairy & Animal Science
K. T. Olgac, M. B. Tirpan, A. Sonat, F. Korkmaz, Ergun Akcay
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Meeting Abstract
Agriculture, Dairy & Animal Science
Beste Cil, Calogero Stelletta, Koray Tekin, Kumsal Duzgun, Ali Daskin, Ergun Akcay
ANIMAL REPRODUCTION SCIENCE
(2020)
Article
Veterinary Sciences
Erkut Dervis Ozgoray, Ergun Akcay
ACTA VETERINARIA EURASIA
(2020)
Article
Veterinary Sciences
Cigdem Cebi Sen, Koray Tekin, Beste Cil, Ergun Akcay
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
(2019)
Meeting Abstract
Agriculture, Dairy & Animal Science
H. Alemdar, A. Daskin
REPRODUCTION IN DOMESTIC ANIMALS
(2018)
Article
Veterinary Sciences
Mehmet Borga Tirpan, Hakan Gurler, Kemal Tuna Olgac, Ali Daskin
ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
(2018)