Review
Cell Biology
Marcela Angelica De la Fuente-Hernandez, Miguel Angel Sarabia-Sanchez, Jorge Melendez-Zajgla, Vilma Maldonado-Lagunas
Summary: In recent years, it has been recognized that long noncoding RNAs (lncRNAs) play a crucial role in cell differentiation processes, particularly in adipogenesis, chondrogenesis, myogenesis, and osteogenesis. Understanding the role of lncRNAs in the signaling network involved in these differentiation processes helps to elucidate the mechanisms of cell differentiation.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jianfang Wang, Qiaoling Zhu, Dandan Cao, Qiqi Peng, Xiaoying Zhang, Chong Li, Chen Zhang, Bo O. Zhou, Rui Yue
Summary: This study identifies bone marrow stromal cells (BMSCs) and megakaryocytes/platelets (MKs/PLTs) as two important sources of IGF-1 that coordinate to maintain and regenerate the adult skeleton, highlighting reciprocal regulation between the hematopoietic and skeletal systems.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Gerard Boix-Lemonche, Richard M. Nagymihaly, Xhevat Lumi, Goran Petrovski
Summary: This research demonstrates the trilineage differentiation potential of human lens cells and reveals the risks of complications during cataract surgery, such as calcification and osteogenic differentiation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Hisaya Kato, Yoshiro Maezawa, Naoya Takayama, Yasuo Ouchi, Hiyori Kaneko, Daisuke Kinoshita, Aki Takada-Watanabe, Motohiko Oshima, Masaya Koshizaka, Hideyuki Ogata, Yoshitaka Kubota, Nobuyuki Mitsukawa, Koji Eto, Atsushi Iwama, Koutaro Yokote
Summary: Werner syndrome (WS) is characterized by accelerated aging symptoms in the limbs, with distinct gene expression and differentiation capabilities observed in fibroblasts from the feet compared to the trunk, which may partially explain the underlying mechanism behind this disease phenotype.
Review
Cell Biology
Chao Yang, Maowen Luo, Yu Chen, Min You, Qiang Chen
Summary: MicroRNAs play a critical role in regulating the differentiation of mesenchymal stem cells, providing clues for therapeutic strategies in regenerative medicine and biotherapy for related diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Cheng-Fong Chen, Yi-Chun Chen, Yu-Show Fu, Shang-Wen Tsai, Po-Kuei Wu, Chao-Ming Chen, Ming-Chau Chang, Wei-Ming Chen
Summary: This study explored the potential of revitalizing human decellularized allogeneic bone with human mesenchymal stem cells for osteochondral regeneration. Results showed that bone marrow and adipose tissue-derived MSCs exhibited higher osteogenic potential, while Wharton's jelly-derived MSCs had higher chondrogenic potential. Chondrogenic MSCs-seeded allogeneic bone may serve as potential osteochondral constructs for regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ruediger Hardeland
Summary: Melatonin interacts with various types of stem cells in multiple ways, stimulating proliferation, maintenance of stemness and self-renewal, protecting survival, and programming toward different cell lineages. The signaling pathways and factors involved in melatonin's effects on stem cells are discussed, including the role of Wnt pathway and microenvironment in developmental decisions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zun Wang, Xiaohua Li, Junxiao Yang, Yun Gong, Huixi Zhang, Xiang Qiu, Ying Liu, Cui Zhou, Yu Chen, Jonathan Greenbaum, Liang Cheng, Yihe Hu, Jie Xie, Xucheng Yang, Yusheng Li, Martin R. Schiller, Yiping Chen, Lijun Tan, Si-Yuan Tang, Hui Shen, Hong-Mei Xiao, Hong-Wen Deng
Summary: This study utilized single-cell RNA sequencing to identify distinct subpopulations of BM-MSCs in humans, shedding light on their cellular heterogeneity and roles in maintaining bone homeostasis, as well as potential novel markers for BM-MSC purification.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Orthopedics
Hong-Bin Yu, Jing Xiong, Hui-Zhen Zhang, Qin Chen, Xu-Yong Xie
Summary: Treating tendon fibrosis with TGF beta 1-transfected TSCs may be an effective approach.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Chemistry, Physical
Nhat Tien Tran, Yu-Kyoung Kim, Seo-Young Kim, Min-Ho Lee, Kwang-Bok Lee
Summary: This study compared the biodegradation and osteogenesis of magnesium (Mg) implants in the epiphysis and diaphysis of long bones in a rat model. The results showed that the epiphyseal region had a better response to Mg implants, with higher new bone formation and better integration of the bone with the implants.
Article
Cell & Tissue Engineering
Rose Ann G. Franco, Eamonn McKenna, Pamela G. Robey, Ross W. Crawford, Michael R. Doran, Kathryn Futrega
Summary: For the use of bone marrow stromal cells (BMSC) in cartilage repair, controlling their hypertrophic differentiation is crucial. TGF-beta 1 stimulation triggers chondrogenic and hypertrophic differentiation in BMSCs, and silencing SP7, a transcription factor associated with hypertrophic differentiation, reduces both hypertrophic and chondrogenic differentiation processes. However, SP7 silencing compromises overall trilineage differentiation of BMSCs and makes it challenging to promote chondrogenesis while reducing hypertrophy in cartilage tissue engineering.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Maria Teresa Valenti, Alessandro Matte, Enrica Federti, Mark Puder, Lorenzo Anez-Bustillos, Michela Deiana, Samuele Cheri, Arianna Minoia, Carlo Brugnara, Maria Luisa Di Paolo, Luca Dalle Carbonare, Lucia De Franceschi
Summary: Sickle cell disease is a genetic disorder causing chronic anemia and organ damage. Study shows that Omega-3 fatty acids can improve osteogenesis, reduce inflammation, and promote adipose tissue transformation, offering new insights for the treatment of SBD.
Article
Engineering, Multidisciplinary
Hyouk-Keun Jee, Woo-Young Jeon, Han-Wool Kwak, Hyun Seok
Summary: Recombinant human bone morphogenetic protein-2 (rhBMP-2) can induce osteogenesis and adipogenesis in bone scaffolds. In this study, the long-term changes in adipose tissue as well as new bone formation in different scaffold forms were evaluated. The results showed that rhBMP-2 effectively increased bone mineral density and trabecular thickness in the particle group over time and led to a decrease in adipose tissue volume. This suggests that rhBMP-2 can effectively induce new bone formation in particle bone scaffolds.
Article
Polymer Science
Ilnur Ishmukhametov, Svetlana Batasheva, Elvira Rozhina, Farida Akhatova, Rimma Mingaleeva, Artem Rozhin, Rawil Fakhrullin
Summary: This study demonstrates that substrates modified with magnetic nanoparticles and DNA can induce the differentiation of human adipose-derived MSCs into chondrocytes and osteoblasts. An increase in the concentration of magnetic nanoparticles leads to reduced cell adhesion and increased surface roughness.
Article
Endocrinology & Metabolism
Ziyi Li, Yafei Wang, Shilun Li, Yukun Li
Summary: Bone regeneration is a complex process involving the coordination of osteogenesis and osteoclastogenesis, with the balance between osteogenesis and adipogenesis of BMSCs playing a critical role. Recent studies have shown that exosomes from M2 macrophages facilitate osteogenesis and reduce adipogenesis through the miR-690/IRS-1/TAZ axis, potentially serving as a therapeutic tool for bone loss diseases.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Editorial Material
Endocrinology & Metabolism
Jillian Cornish, Richard O. C. Oreffo
Article
Multidisciplinary Sciences
Jennifer M. Dewingid, Vinay Saunders, Ita O'Kelly, David I. Wilsonid
Summary: This study investigated the expression and specificity of commonly used cardiac cell markers in the early human fetal heart. The findings suggest differences in cellular composition between early fetal heart and adult heart.
Article
Biochemistry & Molecular Biology
Hannah L. L. Smith, Juliet C. C. Gray, Stephen A. A. Beers, Janos M. M. Kanczler
Summary: Osteosarcoma, a bone cancer typically found in adolescents, is closely related to the conversion of bone marrow during adolescence. This study compared the differentiation potential of bone marrow cells from different regions with osteosarcoma cell lines. The results showed distinct differences in the differentiation abilities of hematopoietic and adipocyte-rich bone marrow, which correlated with specific characteristics of the osteosarcoma cell lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
Antonio Conde-Gonzalez, Michael Glinka, Deepanjalee Dutta, Robert Wallace, Anthony Callanan, Richard O. C. Oreffo, Mark Bradley
Summary: Regenerative medicine demands sophisticated 3D biomaterials to promote tissue formation. This study presents a novel technique for screening potential biocompatible 3D scaffolds, identifying a porous acrylate-based scaffold that promotes cell proliferation and tissue formation. The scaffold was fabricated through freeze-casting and photo-polymerization techniques and demonstrated tunable properties suitable for skeletal repair. This platform offers an innovative approach to scanning synthetic biomaterials for tissue regeneration.
BIOMATERIALS ADVANCES
(2023)
Article
Chemistry, Multidisciplinary
Roxanna S. Ramnarine-Sanchez, Janos M. Kanczler, Nicholas D. Evans, Richard O. C. Oreffo, Jonathan I. Dawson
Summary: This paper reports a new reaction-diffusion self-assembly system, in which proteins function as diffusive reactants to assemble stable clay-protein composite hydrogels. Using this method, scalable and highly stable 3D protein patterns can be generated at sub-cellular resolution, and can guide cell behavior to achieve precise bone tissue template formation.
ADVANCED MATERIALS
(2023)
Article
Biochemical Research Methods
Duanyang Xu, Konstantinos N. Bourdakos, Anna Crisford, Peter Johnson, Ibrahim Abughazaleh, Panuwat Srisamran, Richard O. C. Oreffo, Sumeet Mahajan, David J. Richardson, Lin Xu
Summary: We present an all-fiber ized 1840-nm thulium-fiber-laser source for label-free biological imaging through nonlinear microscopy. The source consists of a dissipative-soliton mode-locked seed laser and a chirped-pulse-amplification system. It utilizes short pulses generated by the mode-locked laser and pulse compression techniques to achieve high-quality imaging of biological samples.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Cell & Tissue Engineering
Elloise Z. Matthews, Stuart Lanham, Kate White, Maria-Eleni Kyriazi, Konstantina Alexaki, Afaf H. El-Sagheer, Tom Brown, Antonios G. Kanaras, Jonathan West, Ben D. MacArthur, Patrick S. Stumpf, Richard O. C. Oreffo
Summary: Researchers have developed a new method to isolate and enrich skeletal stem cells from human bone marrow, which have the potential to differentiate into bone, cartilage, and fat cells. This study provides valuable insights for further understanding and utilization of these stem cells.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Cell & Tissue Engineering
Cameron Black, David Gibbs, Josephine McEwan, Janos Kanczler, Marta Pena Fernandez, Gianluca Tozzi, Jonathan Dawson, Richard Oreffo
Summary: Development of appropriate growth factor delivery strategies and preclinical models are crucial for bone tissue regeneration. This study investigated the effect of a growth factor delivery system combined with Laponite gel on bone formation in a sheep model. The results showed that Laponite had excellent biocompatibility and the ability to deliver active BMP-2, indicating its potential for bone fracture therapeutics.
JOURNAL OF TISSUE ENGINEERING
(2022)
Meeting Abstract
Cell & Tissue Engineering
Yang-Hee Kim, Gianluca Cidonio, Juan Aviles Milan, Richard O. C. Oreffo, Jonathan I. Dawson
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Yang-Hee Kim, Agnieszka Janeczek, Richard O. C. Oreffo, Jonathan I. Dawson
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Roxanna Ramnarine Sanchez, Nicholas Evans, Richard Oreffo, Jonathan Dawson
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Yuanhao Wu, Babatunde O. Okesola, John Connelly, Richard O. C. Oreffo, Jose Carlos Rodriguez-Cabello, Giovanni Vozzi, Helena S. Azevedo, Nicola M. Pugno, Alvaro Mata
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Gianluca Cidonio, Federico Salaris, Janos Kanczler, Alessandro Soloperto, Chiara Scognamiglio, Andrea Barbetta, Jonathan Dawson, Richard Oreffo, Alessandro Rosa, Giancarlo Ruocco
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Abshar Hasan, Karen Marshall, Jonathan Wojciechowski, Sherif Elsharkawy, David Eglin, Richard Oreffo, Molly Stevens, Alvaro Mata
TISSUE ENGINEERING PART A
(2022)
Review
Biotechnology & Applied Microbiology
Yang-Hee Kim, Jonathan Dawson, Richard O. C. Oreffo, Yasuhiko Tabata, Dhiraj Kumar, Conrado Aparicio, Isha Mutreja
Summary: Musculoskeletal disorders pose a significant burden on the global economy and public health. Hydrogels, especially Gelatin methacryloyl (GelMA) hydrogel, hold great potential for enhancing the repair of damaged musculoskeletal tissues. This review provides a comprehensive overview of GelMA synthesis, hydrogel design, and its applications in regenerative medicine, particularly in the field of musculoskeletal drug delivery.
BIOENGINEERING-BASEL
(2022)