Article
Cell & Tissue Engineering
Shune Xiao, Chunfang Xiao, Yong Miao, Jin Wang, Ruosi Chen, Zhexiang Fan, Zhiqi Hu
Summary: The study demonstrated that hAAM scaffolds incorporated with ADSC-Exos can promote diabetic skin wound healing by regulating inflammation, stimulating vascularization, and promoting the production of extracellular matrix.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Medicine, Research & Experimental
Shaghayegh Doudi, Mansoureh Barzegar, Elham Angouraj Taghavi, Mohammad Eini, Arian Ehterami, Karen Stokes, Jonathan S. Alexander, Majid Salehi
Summary: The human amniotic membrane (hAM) has wide applications in regenerative medicine and shows advantages in multiple fields. This review summarizes the recent applications of hAM in various domains and discusses the pros and cons of different methods for hAM preparation.
Article
Biochemistry & Molecular Biology
Dilcele Silva Moreira Dziedzic, Bassam Felipe Mogharbel, Ana Carolina Irioda, Priscila Elias Ferreira Stricker, Maiara Carolina Perussolo, Celia Regina Cavichiolo Franco, Hsueh-Wen Chang, Eltyeb Abdelwahid, Katherine Athayde Teixeira de Carvalho
Summary: The study demonstrates that using Decellularized Human Amniotic Membrane (DAM) with adipose-derived stromal cells (ASCs) and a mineralized extracellular matrix (ECM) in treating rat periodontal furcation defect model can effectively promote bone tissue healing and support periodontal regeneration.
Article
Materials Science, Multidisciplinary
Sudha Varadaraj, Subramani Kandhasamy, Sangeetha Kandoi, Janani Radhakrishnan, Pushpavanam Subramaniam, Rama Shanker Verma
Summary: Human Amniotic Membranes (hAM) serve as important components in tissue engineering due to their biocompatibility, low cost, and availability. Recent research has focused on using hAM as a potential source of composite biomaterials by retaining growth factors and secretome. In this study, decellularized, dehydrated Amniotic Membrane (dAM) incorporated with embelin, a natural benzoquinone compound, was used to neutralize free radicals and accelerate wound healing. The study showed that the embeling-incorporated dAM not only retained the bioactivity of growth factors, but also significantly enhanced fibroblast cell adhesion and proliferation, leading to rapid wound healing and tissue regeneration.
MATERIALS TODAY COMMUNICATIONS
(2022)
Review
Biotechnology & Applied Microbiology
Kaiyang Liu, Yunfan He, Feng Lu
Summary: This review discusses recent advancements in understanding the behavior of inflammatory cells during the regeneration of acellular adipose matrix (AAM). The findings will inform the development of AAM products with enhanced properties.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jiangjiang Ru, Qian Zhang, Shaowei Zhu, Junrong Cai, Yunfan He, Feng Lu
Summary: The study found that HEP-ADGF-AAM, a surface-modified heparin-loaded acellular adipose matrix (AAM) containing adipose-derived growth factors (ADGFs), can promote wound healing. HEP-ADGF-AAM not only induced adipocyte regeneration but also enhanced fibroblast migration, promoted vessel formation, accelerated wound closure, and enhanced wound epithelialization.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Veterinary Sciences
Danica D. Wolkowski, Robert D. McCarthy, Mike J. Schoonover, Jared D. Taylor, Timothy G. Eastman
Summary: This study evaluated the effects of injecting acellular equine liquid amnion allograft (ELAA) into healthy horse joints. The results showed an increase in cell count and IL-1ra level in the treated joints, but no lameness was observed. This suggests that intra-articular injection of ELAA in healthy equine joints is safe and may have beneficial effects on inflamed joints.
VETERINARY SURGERY
(2023)
Article
Biology
Gustavo Gavazzoni Blume, Paulo Andre Bispo Machado-Junior, Rossana Baggio Simeoni, Giovana Paludo Bertinato, Murilo Sgarbossa Tonial, Seigo Nagashima, Ricardo Aurino Pinho, Lucia de Noronha, Marcia Olandoski, Katherine Athayde Teixeira de Carvalho, Julio Cesar Francisco, Luiz Cesar Guarita-Souza
Summary: The study suggests that cell-based therapies using bone marrow stem cells and human amniotic membrane can effectively improve ventricular function and volume in heart failure rat models after myocardial infarction, increasing angiogenesis and cardiomyocyte expression.
Article
Gastroenterology & Hepatology
Bing Han, Maria Giovanna Francipane, Amin Cheikhi, Joycelyn Johnson, Fei Chen, Ruoyu Chen, Eric Lagasse
Summary: The study demonstrates that hepatocytes transplanted via intraperitoneal injection can engraft and generate ectopic liver tissues in fat-associated lymphoid clusters (FALCs), rescuing mice from liver failure. Gene expression in ectopic livers shows selective compensatory changes in hepatic function, indicating functional integration between the two liver sites.
Article
Biochemistry & Molecular Biology
Sun A. Ock, Seo-Yeon Kim, Won Seok Ju, Young-Im Kim, Ha-Yeon Wi, Poongyeon Lee
Summary: In this study, hepatocyte organoids (HOs) were generated using frozen-thawed primary hepatocytes (PHs) in a three-dimensional (3D) culture system. By co-culturing with adipose tissue-derived mesenchymal stem cells (A-MSCs), the HOs showed improved liver function and increased in vitro lifespan compared to conventional two-dimensional (2D) culture. This improvement makes the HOs suitable for large-scale human hepatotoxicity testing and precise livestock nutrition assessment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Anatomy & Morphology
Davood Nasiry, Ali Reza Khalatbary, Afshin Noori, Beheshteh Abouhamzeh, Zahra Jamalpoor
Summary: The lack of suitable scaffold and blood supply, as well as the shortage of growth factors, are the main factors contributing to non-optimal healing of diabetic wounds. In this study, a combination of a bioengineered decellularized amniotic membrane-scaffold and adipose-derived stem cells (ASCs) was investigated for its potential in promoting healing in ischemic wounds in type 1 diabetic rats. The results showed that the treatment groups had significantly higher rates of wound closure, volumes of new epidermis and dermis, densities of fibroblasts and blood vessels, numbers of proliferating cells and collagen deposition, as well as better biomechanical properties of the healed wounds compared to the untreated group. The DAMS+ASC group exhibited the most favorable outcomes. Overall, using both DAMS engraftment and ASC transplantation had a greater impact on diabetic wound healing.
Article
Endocrinology & Metabolism
Dilcele Silva Moreira Dziedzic, Julio Cesar Francisco, Bassam Felipe Mogharbel, Ana Carolina Irioda, Priscila Elias Ferreira Stricker, Juliana Floriano, Lucia de Noronha, Eltyeb Abdelwahid, Celia Regina Cavichiolo Franco, Katherine Athayde Teixeira de Carvalho
Summary: Decellularized Human Amniotic Membrane (DAM) combined with Adipose-derived stromal cells (ASCs) were used as a scaffold for bone regeneration in rat calvarial defects. The results showed that DAM and ASCs combined treatment enhanced bone deposition and healing of non-healing calvarial defects.
CALCIFIED TISSUE INTERNATIONAL
(2021)
Article
Dermatology
Sima Davoodi, Roohollah Ebrahimpour-Malekshah, Omer Ayna, Mitra Akbari, Amir Raoofi, Hossein Mokhtari, Mostafa Izanlu, Faezeh Modanloo, Davood Nasiry
Summary: The study demonstrated that the concurrent use of dHAM and ADS can effectively accelerate the healing of diabetic wounds and improve the quality of wound repair. The treated groups showed increased volumes of newly formed epidermis and dermis, density of fibroblasts and blood vessels, collagen deposition, density of proliferation cells, expression levels of genes, and improved biomechanical characteristics.
JOURNAL OF COSMETIC DERMATOLOGY
(2022)
Review
Pharmacology & Pharmacy
Yanqi Li, Siyu An, Chengliang Deng, Shune Xiao
Summary: Human acellular amniotic membrane (HAAM) has emerged as a promising tool in regenerative medicine with low immunogenicity and good anti-infective and anti-scarring results. This review provides a comprehensive overview of HAAM, including its preparation, sterilization, preclinical research, and clinical applications. The review also discusses the potential of HAAM as a skin dressing and cell delivery system and the need for further research into its regenerative mechanisms.
Article
Biochemistry & Molecular Biology
Massimo Conese, Ottavio Napolitano, Onofrio Laselva, Sante Di Gioia, Luigi Nappi, Luigia Trabace, Maria Matteo
Summary: This study found that the levels of PLAC1 in AMSCs were lower in patients with preeclampsia, and abnormal shedding of PLAC1 from AMSC plasma membranes may contribute to trophoblast proliferation and support its role in the development of preeclampsia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Xinyue Guo, Weihong Li, Minghui Ma, Xin Lu, Haiyan Zhang
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2017)
Article
Neurosciences
Beibei Wang, Xifeny Zou, Haiyan Zhang, Deyi Duan, Lili Ju, Xiaohua Jiang, Xiaohong Sun, Chunli Zhao, Huanying Zhao, Jin Guo, Changlei Xu, Erjing Gao, Qunyuan Xu
Article
Cell Biology
Mingxi Hua, Weitao Zhang, Weihong Li, Xueyang Li, Baoqing Liu, Xin Lu, Haiyan Zhang
JOURNAL OF CELL SCIENCE
(2012)
Article
Cell Biology
Weitao Zhang, Weihong Li, Baoqing Liu, Ping Wang, Wen Li, Haiyan Zhang
JOURNAL OF CELLULAR PHYSIOLOGY
(2012)
Article
Cell Biology
Xueyang Li, Jie Yuan, Weihong Li, Sicheng Liu, Mingxi Hua, Xin Lu, Haiyan Zhang
JOURNAL OF CELLULAR PHYSIOLOGY
(2014)
Article
Neurosciences
Haiyan Zhang, Yisong Wang, Yongmei Zhao, Yanling Yin, Qiuyan Xu, Qunyuan Xu
JOURNAL OF NEUROSCIENCE RESEARCH
(2008)
Article
Cell Biology
Yuejiao Ma, Minghui Ma, Jie Sun, Weihong Li, Yaqiong Li, Xinyue Guo, Haiyan Zhang
JOURNAL OF CELL SCIENCE
(2019)
Article
Multidisciplinary Sciences
Yaqiong Li, Yi Lin, Xueya Han, Weihong Li, Wenmao Yan, Yuejiao Ma, Xin Lu, Xiaowu Huang, Rixing Bai, Haiyan Zhang
Summary: The study found that inhibiting GSK3 can enhance mitochondrial function in hepatocytes of obese patients, thereby improving hepatic steatosis and reducing lipid droplet size and triglyceride levels in hepatocytes.
Article
Neurosciences
HY Zhang, YM Zhao, CL Zhao, S Yu, DY Duan, QY Xu
NEUROSCIENCE RESEARCH
(2005)