Journal
SKIN RESEARCH AND TECHNOLOGY
Volume 15, Issue 4, Pages 418-426Publisher
WILEY
DOI: 10.1111/j.1600-0846.2009.00381.x
Keywords
two-photon excitation fluorescence (TPEF) excitation-emission matrices (EEM); second harmonic generation (SHG); collagen; elastin; skin; multiphoton microscopy
Categories
Funding
- Canadian Dermatology Foundation
- Canadian Institutes of Health Research/National Natural Science Foundation of China [60711120031, 60508017]
- Natural Science and Engineering Research Council of Canada
- VGH & UBC Hospital Foundation
- BC Hydro Employees Community Services Fund
- BC Cancer Agency
- Natural Science Foundation of Fujian Province of China [2007J0007, C0720001]
- Program for New Century Excellent Talents in University [NCET-07-0191]
- Science and Technology Planning Key Program of Fujian Province [2008Y0037]
Ask authors/readers for more resources
Background/purposes Understanding the two-photon excitation spectral characteristics and microscopic morphology of cutaneous collagen and elastic tissue components is important for applying multiphoton microscopy (MPM) in basic skin biology research and for clinical diagnosis. Methods We developed a system for two-photon excitation spectral measurements at various excitation wavelengths. The microscopic morphology was studied using a commercial multiphoton microscope. Results We obtained two-photon excitation fluorescence (TPEF) excitation-emission matrices (EEM), for the first time, of purified collagen and elastin samples, as well as in situ collagen and elastic fibers within excised human dermis. The EEM of the dermis was found to be similar to that of elastin. The excitation spectra for second harmonic generation (SHG) from purified collagen and excised dermis were also studied and were found to have similar spectral shapes. Conclusion This study, using the EEM spectroscopic approach, confirmed a previous imaging study inference that in the dermis, TPEF predominantly originates from elastic fibers, while SHG originates solely from collagen fibers. The EEM data and SHG excitation spectra obtained in this study can be used to guide the selection of excitation wavelengths for MPM applications in basic skin biology research and for clinical diagnosis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available