Journal
SKIN PHARMACOLOGY AND PHYSIOLOGY
Volume 23, Issue 6, Pages 320-327Publisher
KARGER
DOI: 10.1159/000313514
Keywords
Hair follicle; Follicle closing technique; Franz diffusion cell; Caffeine; Penetration
Categories
Funding
- Skin Physiology Foundation of the Donors' Association for the Promotion of Sciences and Humanities in Germany
Ask authors/readers for more resources
The Organization for Economic Cooperation and Development (OECD) recommends caffeine as a reference substance for in vitro skin absorption tests using Franz diffusion cells (FDC). However, it has not been possible to investigate the follicular penetration pathway using this method until now. The aim of this study was to develop a technique to allow the examination of the follicular penetration pathway of a substance penetrating into the skin. The OECD standard method was therefore combined with the follicle closing technique (FCT), an established in vivo method. By using test skin of varying follicular densities, different penetration values were obtained for the test substance caffeine. The follicular penetration rate was determined by an indirect calculation after modifying the in vivo FCT for use in the in vitro FDC. This method is the first to allow the differentiation of penetration pathways by combining the OECD standard method (using the FDC) and the FCT. Caffeine showed a surprisingly high rate of penetration through the follicular shunts in vitro. Copyright (C) 2010 S. Karger AG, Basel
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available