Review
Chemistry, Multidisciplinary
Junyun Cheng, Gaole Lin, Tianhao Wang, Yunzhu Wang, Wenbo Guo, Jie Liao, Penghui Yang, Jie Chen, Xin Shao, Xiaoyan Lu, Ling Zhu, Yi Wang, Xiaohui Fan
Summary: CRISPR-based genetic screening is a powerful approach for unbiased functional genomics research. Single-cell CRISPR screening techniques allow the analysis of regulatory networks in complex biological systems at unprecedented resolution. These methods involve perturbing cells en masse using a pooled CRISPR library and high-content phenotyping.
Article
Multidisciplinary Sciences
Lea Jessica Flitsch, Kathleen Boerner, Christian Stuellein, Simon Ziegler, Vera Sonntag-Buck, Ellen Wiedtke, Vesselina Semkova, Si Wah Christina Au Yeung, Julia Schlee, Mohamad Hajo, Mona Mathews, Beatrice Stefanie Ludwig, Susanne Kossatz, Horst Kessler, Dirk Grimm, Oliver Bruestle
Summary: Human brain cells generated by in vitro cell programming have potential applications in disease modeling, drug discovery, and cell therapy. A study discovered that recombinant adeno-associated viruses (AAVs) are suitable tools for genetic modification of these cells, with various AAV capsid variants identified as effective in different types of human neural cells. The study provides a comparison of AAV transduction susceptibility in different brain cell types and discusses the advantages and limitations of this screening approach.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Jaewoo Son, Jun Hong Jang, In Hyeok Choi, Chang Gyu Lim, Eun Jung Jeon, Hyun Bae Bang, Ki Jun Jeong
Summary: This study successfully engineered Corynebacterium glutamicum as a whole-cell catalyst for the bioconversion of l-phenylalanine into trans-cinnamic acid (t-CA) with high efficiency, reaching a production yield of 75% through repetitive supplementation. The developed bioconversion process and micromembrane-based cell recycling system provide a promising platform for industrial-scale production of t-CA using microorganisms.
MICROBIAL CELL FACTORIES
(2021)
Article
Multidisciplinary Sciences
Natalie Boehnke, Joelle P. Straehla, Hannah C. Safford, Mustafa Kocak, Matthew G. Rees, Melissa Ronan, Danny Rosenberg, Charles H. Adelmann, Raghu R. Chivukula, Namita Nabar, Adam G. Berger, Nicholas G. Lamson, Jaime H. Cheah, Hojun Li, Jennifer A. Roth, Angela N. Koehler, Paula T. Hammond
Summary: This study used an integrated genomic approach to investigate the association between cancer cells and nanoparticles, identifying both materials properties and cell-intrinsic features that mediate nanoparticle-cell association. The study also identified nanoparticle-specific biomarkers and validated one biomarker for lipid-based nanoparticle uptake. The findings contribute to the rational design of nanoformulations.
Article
Chemistry, Multidisciplinary
Hyunsik Hong, Sunhong Min, Sagang Koo, Yunjung Lee, Jinho Yoon, Woo Young Jang, Nayeon Kang, Ramar Thangam, Hyojun Choi, Hee Joon Jung, Seong-Beom Han, Qiang Wei, Seung-Ho Yu, Dong-Hwee Kim, Ramasamy Paulmurugan, Woong Kyo Jeong, Ki-Bum Lee, Taeghwan Hyeon, Dokyoon Kim, Heemin Kang
Summary: This study investigates the modulation of stem cell differentiation in the microenvironment using nanoassembly-based magnetic screens. Different sizes of screens can affect the adhesion, mechanotransduction, and differentiation of stem cells by adjusting nanogaps. This regulation mechanism is shown to be effective in vivo, demonstrating the potential for diverse modalities of unscreening RGDs to modulate stem cell differentiation for tissue repair.
ADVANCED MATERIALS
(2022)
Article
Food Science & Technology
Fabian Ostertag, Eva Krolitzki, Sonja Berensmeier, Jorg Hinrichs
Summary: Cross-flow filtration is a product-preserving and energy-saving method for producing whey protein concentrates from acid whey. Different organic membranes were compared for their flux and retention characteristics, showing notable differences in hydraulic resistance and deposit layer resistance. The protein retention of all ultrafiltration membranes was below 5%, while microfiltration membranes showed a deposit layer-dependent transmission behavior. The overall filtration efficiency, in terms of process duration for a comparable membrane area, was three times higher compared to conventional production methods.
INTERNATIONAL DAIRY JOURNAL
(2023)
Article
Engineering, Chemical
Xiaolei Zhang, Jialin Huang, Xiaoying Cheng, Huihui Chen, Qiang Liu, Ping Yao, Hao H. Ngo, Long D. Nghiem
Summary: In this study, a hybrid electrochemical-microfiltration-granular activated carbon adsorption (e-MF-GAC) pretreatment process was developed and demonstrated for controlling reverse osmosis (RO) fouling. Compared to ultrafiltration (UF), e-MF-GAC pretreatment resulted in 30% higher permeate flux. Results in this study validate the hypothesis of complementarity among individual treatment processes of e-MF-GAC for removing foulants, leading to superior performance over conventional UF pretreatment.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Engineering, Environmental
Hee-Jun Kim, Uje Lee, Inho Lee, Youngpyo Hong, Jaewoo Lee
Summary: Various sizes of microplastics (MPs) have been found in water bodies, with small MPs (<1μm) posing a threat to human health. Efforts have been made to remove MPs, but previous methods only achieved partial reductions. This study demonstrates that near dissolved organic matter microfiltration (NDOM MF) coupled with cold plasma (CP) treatment can efficiently remove MPs. CP treatment caused coagulation, increasing the particle size of MPs and enabling NDOM MF to achieve a higher water flux and maintain a high removal rate. The NDOM MF-CP process provides a sustainable and eco-friendly approach to removing MPs without secondary contamination.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Chemistry, Physical
Boran Zhang, Siyuan Zou, Wenshuai Wu, Fan Yang, Kashif Mehmood, Wuyi Wan, Qianbin Zhao
Summary: This study used a multiphysics numerical model to investigate the deformation process of cells in a filtration device, revealing the general cellular creep deformation process of viscoelastic particle trapping and quantitatively describing the evolution using two characteristic variables. The study also conducted a Giardia concentration experiment, and the experimental results agreed well with the numerical analysis, indicating that releasing cellular material matters is more difficult due to time-accumulated creep deformation compared to elastic particles.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Maria E. Weinberger, Ulrich Kulozik
Summary: Cell separation from biomolecules is a challenging task in biotechnological operations. This study proposes a screening method using analytical centrifugation to detect small differences in compression and relaxation behavior. The stabilizing role of bovine serum albumin in cell deposits formed at low pH values is underlined.
Editorial Material
Genetics & Heredity
Bridget K. Wagner
Summary: A new candidate gene for type 2 diabetes has been identified through a genome-wide CRISPR screen for intracellular insulin content in a human beta-cell line, demonstrating the usefulness of this screening approach in beta-cells.
Article
Engineering, Environmental
Elshan Bonyadi, Farzin Zokaee Ashtiani, Shima Ghorabi, Ali Sallakh Niknejad
Summary: This study successfully enhanced the hydrophilicity and anti-fouling properties of PES membrane using modification technology, demonstrating high filtration performance and efficiency in oily wastewater treatment.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Alex M. Yoshikawa, Alexandra E. Rangel, Liwei Zheng, Leighton Wan, Linus A. Hein, Amani A. Hariri, Michael Eisenstein, H. Tom Soh
Summary: Aptamer-based molecular switches have various applications such as imaging metabolites, drug delivery, and biomolecule detection. Conventional aptamer selection methods do not produce aptamers with structure-switching functionality, so post-selection modification is necessary. This study presents a screening-based strategy that can convert any aptamer into a molecular switch without prior knowledge of its structure.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Chemical
Hao Peng, Vatsal Shah, K. Li
Summary: The CCD method has shown superiority in producing high-performance PVDF membranes, with the ability to tailor membrane properties by changing the cooling temperature. Increasing the cooling temperature results in larger separation surface pore sizes and higher membrane permeances, especially for membranes prepared using higher molecular weight PVDF.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Review
Environmental Sciences
Hang Liu, Ziqi Li, Zhimin Qiang, Tanju Karanfil, Min Yang, Chao Liu
Summary: With increasing water reuse as a sustainable water management strategy, antibiotic resistance genes (ARGs) are attracting global attention as emerging contaminants in wastewater. This review improves the understanding of the abundance of cell-associated and non-cell-associated ARGs in wastewaters and the secondary effluents and compares the elimination of ARGs in these two forms by low-pressure and high-pressure membrane filtration processes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Meeting Abstract
Oncology
Wolf-Ruprecht Wiedemeyer, Dongping Qi, Navjot Kaur Gi, Chintda Santiskulvong, Oliver Dorigo, JianYu Rao, Barbie Taylor-Harding, Amy C. Rowat
Meeting Abstract
Biophysics
Dongping Qi, Amy Rowat
BIOPHYSICAL JOURNAL
(2012)
Article
Chemistry, Physical
D. Qi, M. Ilton, J. A. Forrest
EUROPEAN PHYSICAL JOURNAL E
(2011)
Review
Physics, Multidisciplinary
D. Qi, D. J. Hoelzle, A. C. Rowat
EUROPEAN PHYSICAL JOURNAL-SPECIAL TOPICS
(2012)
Article
Polymer Science
M. Ilton, D. Qi, J. A. Forrest
Article
Physics, Multidisciplinary
D. Qi, Z. Fakhraai, J. A. Forrest
PHYSICAL REVIEW LETTERS
(2008)
Article
Chemistry, Physical
D. Qi, C. R. Daley, Y. Chai, J. A. Forrest
Article
Nanoscience & Nanotechnology
Jiantong Dong, Ryan Y. Zhang, Na Sun, Matthew Smalley, Zipeng Wu, Anqi Zhou, Shih-Jie Chou, Yu Jen Jan, Peng Yang, Lirong Bao, Dongping Qi, Xinghong Tang, Patrick Tseng, Yue Hua, Dianwen Xu, Rueihung Kao, Meng Meng, Xirun Zheng, Ying Liu, Tatyana Vagner, Xiaoshu Chai, Dongjing Zhou, Mengyuan Li, Shih-Hwa Chiou, Guangjuan Zheng, Dolores Di Vizio, Vatche G. Agopian, Edwin Posadas, Steven J. Jonas, Shin-Pon Ju, Paul S. Weiss, Meiping Zhao, Hsian-Rong Tseng, Yazhen Zhu
ACS APPLIED MATERIALS & INTERFACES
(2019)
Article
Biochemical Research Methods
Navjot Kaur Gill, Chau Ly, Kendra D. Nyberg, Linus Lee, Dongping Qi, Bobby Tofig, Mariana Reis-Sobreiro, Oliver Dorigo, JianYu Rao, Ruprecht Wiedemeyer, Beth Karlan, Kate Lawrenson, Michael R. Freeman, Robert Damoiseaux, Amy C. Rowat
Meeting Abstract
Biophysics
Navjot Kaur Gill, Kendra Dee Nyberg, Dongping Qi, Bobby Tofiq, Robert Damoiseaux, Amy C. Rowat
BIOPHYSICAL JOURNAL
(2018)
Meeting Abstract
Chemistry, Multidisciplinary
Zahra Fakhraai, Dongping Qi, James A. Forrest
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2011)
Article
Physics, Multidisciplinary
CF Cheng, DP Qi, SY Teng, M Liu, TQ Jia, ZZ Xu
SCIENCE IN CHINA SERIES G-PHYSICS ASTRONOMY
(2003)
Article
Physics, Applied
CF Cheng, DP Qi, SY Teng, JP Chen, TQ Jia, ZZ Xu
APPLIED PHYSICS LETTERS
(2002)
Article
Physics, Multidisciplinary
NY Zhang, SY Teng, QM Dong, DP Qi, CF Cheng
ACTA PHYSICA SINICA
(2001)