Article
Biophysics
Aynur Abdulla, Zhianan Zhang, Khan Zara Ahmad, Antony R. Warden, Hengyu Li, Xianting Ding
Summary: This study demonstrates a novel antibody functionalized microfluidic chip that can rapidly and accurately capture circulating tumor cells (CTCs) from cancer patients' whole blood. The chip offers a valuable tool for cancer screening, prognosis, and diagnosis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Multidisciplinary Sciences
Marcos Penedo, Keisuke Miyazawa, Naoko Okano, Hirotoshi Furusho, Takehiko Ichikawa, Mohammad Shahidul Alam, Kazuki Miyata, Chikashi Nakamura, Takeshi Fukuma
Summary: The nanoendoscopy-AFM technique allows for imaging of actin fiber 3D maps and membrane inner scaffold 2D nanodynamics inside living cells without affecting cell viability. This overcomes the limitations of current nanoimaging techniques by directly accessing intracellular components with AFM capabilities.
Article
Multidisciplinary Sciences
Sajad Tavakoli, Ali Ghaffari, Zahra Mousavi Kouzehkanan, Reshad Hosseini
Summary: This article introduces a new method for classifying white blood cells using image processing and machine learning, involving nucleus and cytoplasm detection, feature extraction, and classification. The proposed method performs well on multiple datasets and demonstrates better generalization ability compared to traditional models.
SCIENTIFIC REPORTS
(2021)
Article
Computer Science, Information Systems
Dongming Li, Shiyu Yin, Yu Lei, Jingning Qian, Chunxi Zhao, Lijuan Zhang
Summary: This study proposes a U-Net model based on an attention mechanism and dilated convolutions for the segmentation of blood leukocytes. The traditional convolution is replaced by dilated convolution, and an attention mechanism module is combined to refine the adaptive features and improve the segmentation performance. Experimental results show that the proposed model achieves higher segmentation indicators compared to the original U-Net model.
Article
Biochemistry & Molecular Biology
Yomani D. Sarathkumara, Daniel J. Browne, Ashton M. Kelly, David J. Pattinson, Catherine M. Rush, Jeffrey Warner, Carla Proietti, Denise L. Doolan
Summary: This study compared the performance of two widely used whole-blood RNA collection systems, PAXgene and Tempus, in laboratory and suboptimal tropical field conditions. Tempus tubes maintained higher RNA quantity and integrity compared to PAXgene under suboptimal conditions and also showed better stability of mRNA transcripts for reference genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Somayyeh Bakhtiaridoost, Hamidreza Habibiyan, Hassan Ghafoorifard
Summary: This study presents a fast, hemolysis-free, highly efficient blood plasma separation microfluidic device that combines gravitational sedimentation with dielectrophoresis force. Using finite element analysis, the effect of geometrical parameters on the separation process is investigated to achieve optimal design specifications. The device achieves a purity of 99.98% and a relative increase in flow rate compared to previous studies by utilizing a porous Melt-Blown Polypropylene layer and optimizing the dielectrophoresis force.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Zaidon T. Al-aqbi, Salim Albukhaty, Ameerah M. Zarzoor, Ghassan M. Sulaiman, Khalil A. A. Khalil, Tareg Belali, Mohamed T. A. Soliman
Summary: A novel microfluidic device featuring a nano-junction was developed for on-chip filtration and out-chip collection of blood plasma with a high extraction yield; the device effectively removes blood cells and plasma proteins, allowing for the detection of analytes at low concentrations.
Article
Biochemical Research Methods
Xiaoguang Lu, Mahnoush Tayebi, Ye Ai
Summary: The study developed an inertial microfluidics-based cell sorter for high-throughput isolation and enrichment of WBCs, providing an efficient and effective platform for WBC separation. The sorter is designed to enable broad application for size-based inertial cell sorting and can further enrich specific cell types using different microchips with varying cutoff sizes.
Article
Biochemistry & Molecular Biology
Barbara Boczkowska-Radziwon, Piotr Jozef Olbromski, Anna Rogowska, Magdalena Bujno, Marta Mysliwiec, Agnieszka Zebrowska, Dariusz Sredzinski, Barbara Politynska, Marek Z. Wojtukiewicz, Piotr Radziwon
Summary: This study demonstrates that ozone treatment significantly increases the release of microparticles derived from blood and endothelial cells. Although there were statistically significant changes in some coagulation factors, they were mild and did not exceed normal levels.
Article
Multidisciplinary Sciences
Anna Katarzyna Wronska, Agata Kaczmarek, Justyna Sobich, Sylwia Grzelak, Mieczyslawa Irena Bogus
Summary: Invertebrates are increasingly used for studying the immune system, as their innate immunity shows similarity to mammals. This study aimed to develop a protocol for detecting cytokines in Galleria mellonella larvae hemocytes using flow cytometry. The study successfully developed a reproducible protocol by optimizing various steps, which will facilitate further immunological research using insect cells.
Article
Biophysics
Yang Yang, Aofei Liu, Ching -Ting Tsai, Chun Liu, Joseph C. Wu, Bianxiao Cui
Summary: The unintended binding of small-molecule drugs to ion channels can cause arrhythmia and heart failure. However, the low throughput of current intracellular recording techniques limits the assessment of drug toxicity. To address this challenge, we have developed the NcEA technology, which enables robust, parallel, and long-duration recording of intracellular action potentials. This technology has the potential to be used in preclinical drug screening.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Hyunjung Lim, Jae Young Kim, Seunghee Choo, Changseok Lee, Byoung Joe Han, Chae Seung Lim, Jeonghun Nam
Summary: This study demonstrates viscoelastic microfluidic techniques for continuous separation, concentration, and subsequent washing of Candida cells in the blood. Candida cells were successfully separated from the white blood cells and concentrated by 74.6-fold. Finally, Candida cells at extremely low concentrations became detectable after the removal of white blood cells, buffer solution, and further removal of blood lysate and washing.
Article
Multidisciplinary Sciences
Chia-Heng Chu, Ruxiu Liu, Tevhide Ozkaya-Ahmadov, Brandi E. Swain, Mert Boya, Bassel El-Rayes, Mehmet Akce, Mehmet Asim Bilen, Omer Kucuk, A. Fatih Sarioglu
Summary: The device efficiently enriches viable CTCs through negative enrichment and isolates them from clinical samples while effectively depleting white blood cells, demonstrating the potential for standardized blood-based tumor biopsies.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Chemical
Nicholas G. Hallfors, Jeremy C. M. Teo, Peter M. Bertone, Chakra P. Joshi, Ajymurat Orozaliev, Matthew N. Martin, A. F. Isakovic
Summary: This study presents a novel process for refined control of white blood cells' elasticity using a combination of gold nanoparticles and a microelectrode array device. The capture and controlled deformation of white blood cells enriched with gold nanoparticles are demonstrated and quantified, showing enhanced controllability of DEP-related processes.
Article
Engineering, Biomedical
Sumin Kim, Somin Lee, Jungeun Lim, Hyeri Choi, Habin Kang, Noo Li Jeon, Youngsook Son
Summary: The study established a human BBB microvasculature model and found that hBM-MSCs play a crucial role in tight BBB reformation, while astrocytes are important in inducing capillary network maturation.