Article
Materials Science, Multidisciplinary
Dongjie Zhou, Zheng-Wen Nie, Xiang-Shun Cui
Summary: The study revealed that EB1 remains stable during mouse oocyte meiosis, and its knockdown results in spindle disorganization, chromosome misalignment, and missegregation, affecting the number of mature oocytes and reducing the binding of beta-catenin to actin, ultimately influencing chromosomal arrangement.
MICROSCOPY AND MICROANALYSIS
(2021)
Article
Cell Biology
Hao Qin, Yi Qu, Yi-Feng Yuan, Yang-Yang Li, Jie Qiao
Summary: This study revealed that RBM14 is down-regulated in oocytes from old mice and shows overlapped localization patterns with alpha-tubulin in different stages of meiosis. Knockdown of RBM14 may result in symmetric division of oocytes, spindle defects, and chromosome abnormalities. Additionally, RBM14 interacts with endogenous alpha-tubulin and modulates oocyte meiotic maturation by affecting alpha-tubulin acetylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Ying Xu, Chang -Long Xu, Zhong-Feng Xu, Xin-Jie Wang, Hui-Sheng Liang, Zhao-Cheng Zeng, Li-Xin Zeng, Kang-Na Wei, Shu-Zi Deng, Shu-Juan Xie, Jiang Jiang, Yu-Xin Liu, Yun-Kao Cao, Hai-Long Wang
Summary: Fbf1 plays a crucial role in spindle assembly and chromosome arrangement during mouse oocyte meiosis, affecting the progression of cell cycle.
Article
Cell Biology
Lin-Lin Hu, Meng-Hao Pan, Feng-Lian Yang, Zi-Ao Zong, Feng Tang, Zhen-Nan Pan, Xiang Lu, Yan-Ping Ren, Jun-Li Wang, Shao-Chen Sun
Summary: Research demonstrates that FASCIN is crucial for mouse oocyte maturation, affecting spindle migration and polar body extrusion. It may be regulated by PKC and plays a significant role in meiosis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Guangyi Cao
Summary: The correct assembly and regulation of the spindle apparatus are crucial for the precise separation of chromosomes in mouse oocytes. NUSAP1 is a spindle-associated protein that plays an important role in oocyte meiosis maturation. Its depletion leads to chromosome misalignment, aneuploidy, and abnormal spindle assembly.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Cell Biology
Yuan-Jing Zou, Meng-Meng Shan, Hong-Hui Wang, Zhen-Nan Pan, Meng-Hao Pan, Yi Xu, Jia-Qian Ju, Shao-Chen Sun
Summary: Our study investigated the roles of RAB14 during oocyte meiotic maturation, showing that depletion of RAB14 caused large polar bodies and defects in spindle migration. The regulation on actin-based spindle migration and Golgi apparatus distribution by RAB14 during mouse oocyte meiotic maturation was suggested by our results.
CELL PROLIFERATION
(2021)
Article
Cell Biology
Jian Li, Hao-Ya Chang, Zi-Yun Yi, Chun-Hui Zhang, Qing-Yuan Sun, Wei-Ping Qian
Summary: Studies on mammalian oocytes have shown that CDK2 activity plays a crucial role in the proper dynamics of spindle and chromosomes, with CDK2 inhibition leading to continuous SAC activation and subsequent inactivation of APC/C activity.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Zhen-Nan Pan, Jing-Cai Liu, Jia-Qian Ju, Yue Wang, Shao-Chen Sun
Summary: This study demonstrates the critical roles of LRRK2 in mammalian oocyte meiosis, primarily through regulating actin assembly for spindle migration and maintaining mitochondrial function.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jia-Qian Ju, Zhen-Nan Pan, Kun-Huan Zhang, Yi-Ming Ji, Jing-Cai Liu, Shao-Chen Sun
Summary: This study demonstrates that Mcrs1 plays important roles in regulating the meiotic G2/M transition and spindle assembly in mouse oocytes. Mcrs1 is present in the nucleus and associates with spindle poles and chromosomes during meiosis I. Depletion of Mcrs1 affects histone modifications, CDK1 activity, and cyclin B1 accumulation, leading to delayed G2/M transition and abnormal spindle assembly.
Article
Biochemistry & Molecular Biology
Wei Yue, Yue Wang, Tie-Gang Meng, Hong-Yong Zhang, Xin-Ran Zhang, Ying-Chun Ouyang, Yi Hou, Heide Schatten, Zhen-Bo Wang, Qing-Yuan Sun
Summary: This study investigates the role of outer kinetochore protein KNL1 in mouse oocytes and reveals its critical function in ensuring timely and accurate chromosome segregation during oocyte meiotic maturation.
Article
Reproductive Biology
Jie Zhang, Hong-Jie Yuan, Jiang Zhu, Shuai Gong, Ming-Jiu Luo, Jing-He Tan
Summary: Topoisomerase II dysfunction impairs chromatin condensation and chromosome alignment, activates Aurora B, and leads to MI arrest and PB1 abscission failure.
BIOLOGY OF REPRODUCTION
(2022)
Article
Developmental Biology
Karla J. Hutt, Shu Ly Lim, Qing-Hua Zhang, Maria Gonzalez, Anne E. O'Connor, D. Jo Merriner, Seng H. Liew, Usama Al-Zubaidi, Wai Shan Yuen, Deepak Adhikari, Rebecca L. Robker, Jeffrey R. Mann, John Carroll, Moira K. O'Bryan
Summary: Studies have shown that mutant female mice with Henmt1 mutation have a 3- to 4-fold reduction in follicle number and reduced litter sizes. Mutant oocytes display various spindle abnormalities and have a dramatically altered transcriptome, which includes down-regulation of genes required for microtubule function. These effects on oogenesis could be exacerbated by asynapsis, a common feature of piRNA mutants in both sexes.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Cell Biology
Xingzhu Du, Jun Li, Qingrui Zhuan, Luyao Zhang, Lin Meng, Panyu Ren, Xiaohan Huang, Jiachen Bai, Pengcheng Wan, Wenquan Sun, Yunpeng Hou, Shien Zhu, Xiangwei Fu
Summary: Oocyte cryopreservation has significant benefits in conserving animal germplasm resources and assisting reproductive technology. However, this technique can cause damage to oocytes, leading to a decrease in oocyte quality and post-fertilization embryonic development. This study examined oocyte cortical tension and found that vitrified oocytes had decreased cortical tension. Increasing cortical tension improved oocyte polar body extrusion, embryo development, spindle positioning, KT-MT attachment, SAC activity, and reduced aneuploidy in vitrified oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Juan Ge, Na Zhang, Shoubin Tang, Feifei Hu, Xiaojing Hou, Hongzheng Sun, Longsen Han, Qiang Wang
Summary: Maternal diabetes impairs oocyte quality, but controlling the PDK1-mediated Ser232-PDHE1α phosphorylation pathway can partly protect oocytes from the effects of diabetes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Cecilia S. Blengini, Karen Schindler
Summary: This article discusses the basic mechanisms of spindle formation during meiotic maturation, focusing on studies in mice and humans. It also explores various factors that may influence spindle formation and stability, as well as the potential impact of assisted reproductive technologies on spindles and subsequent embryo development.
BIOLOGY OF REPRODUCTION
(2022)