Article
Neurosciences
Young-Geun Choe, Jin-Hui Yoon, Jongyoon Joo, Bokyung Kim, Seon Pyo Hong, Gou Young Koh, Dong-Seok Lee, Wang-Yuhl Oh, Yong Jeong
Summary: The neurovascular unit, consisting of neurons, glial cells, pericytes, and endothelial cells, plays a crucial role in sustaining brain activity. However, the specific function of pericytes in regulating cerebral blood flow remains unclear. This study investigates the impact of pericyte loss on capillary stalling and the potential causes, such as changes in cerebral endothelial glycocalyx and leukocyte adhesion molecules. The results reveal that pericyte loss leads to increased capillary stalling and enhanced interaction between leukocytes and endothelial cells, ultimately resulting in cerebral hypoxia and gliosis.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Cardiac & Cardiovascular Systems
Adam Nyul-Toth, Stefano Tarantini, Jordan DelFavero, Feng Yan, Priya Balasubramanian, Andriy Yabluchanskiy, Chetan Ahire, Tamas Kiss, Tamas Csipo, Agnes Lipecz, Attila E. Farkas, Imola Wilhelm, Istvan A. Krizbai, Qinggong Tang, Anna Csiszar, Zoltan Ungvari
Summary: Age-related blood-brain barrier disruption and cerebromicrovascular rarefaction play important roles in the pathogenesis of vascular cognitive impairment and dementia (VCID) and Alzheimer's disease (AD). Recent advances in geroscience research have provided new interventions for preventing these diseases. Sensitive and easy-to-adapt imaging methods are urgently needed for longitudinal assessment of changes in BBB permeability and brain capillarization in aged mice.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Ravikumar Vaghela, Andreas Arkudas, Raymund E. Horch, Maximilian Hessenauer
Summary: Intravital microscopy (IVM) offers real-time imaging and tracking of cellular events. It plays a significant role in studying tissue regeneration processes and analyzing host reactions to implanted biomaterials.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Neurosciences
Asher A. Mendelson, Stephanie Milkovich, Timothy Hunter, Raashi Vijay, Yun-Hee Choi, Shaun Milkovich, Edward Ho, Daniel Goldman, Christopher G. Ellis
Summary: The capillary module, traditionally thought to be the building block of complex capillary networks, fails to explain how blood flow is regulated in skeletal muscle. The concept of a capillary fascicle, consisting of interconnected modules, plays a crucial role in determining blood flow distribution in skeletal muscle. The findings provide direct evidence for microvascular flow regulation at the level of the capillary module, updating the paradigm for characterizing blood flow and red blood cell distribution in capillary networks.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Biochemistry & Molecular Biology
Jakob R. Kaeppler, Jianzhou Chen, Mario Buono, Jenny Vermeer, Pavitra Kannan, Wei-Chen Cheng, Dimitrios Voukantsis, James M. Thompson, Mark A. Hill, Danny Allen, Ana Gomes, Veerle Kersemans, Paul Kinchesh, Sean Smart, Francesca Buffa, Claus Nerlov, Ruth J. Muschel, Bostjan Markelc
Summary: This study used multiphoton microscopy to investigate the effect of radiation therapy on tumor vasculature. The results showed that there was minimal structural change in the perfused vasculature after radiation therapy, but non-perfused vessels and blind ends were significantly impaired, accompanied by apoptosis and reduced proliferation of endothelial cells.
Article
Immunology
Wagdi Almishri, Rachelle P. Davis, Abdel-Aziz Shaheen, Mohammed O. Altonsy, Craig N. Jenne, Mark G. Swain
Summary: Mirtazapine treatment rapidly alters hepatic B cell populations, enhancing hepatic retention of CXCR3-expressing innate-like B cells that generate a more anti-inflammatory cytokine profile. Mirtazapine-induced hepatic B cell shifts could potentially represent a novel therapeutic approach to immune-mediated liver diseases characterized by B cell driven pathology.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Yu-Hang Liu, Lorenz M. Brunner, Johannes Rebling, Maya Ben-Yehuda Greenwald, Sabine Werner, Michael Detmar, Daniel Razansky
Summary: This study used non-invasive imaging technology to observe and monitor the process of skin repair in mice, and found that overexpression of vascular endothelial growth factor A (VEGF-A) can promote vascularization in the skin, which was further validated by detailed histological staining results.
Article
Anesthesiology
Alexander Fuchs, Tobias Neumann, Hendrik Drinhaus, Anika Herrmann, Hans Vink, Thorsten Annecke
Summary: This study examined the real-time ESL and perfusion measurements of the sublingual microcirculation in healthy subjects before and after a 10 km running distance. The results showed no immediate effect of exercise on PBR and RBC filling percentage, but a significant association between changes in PBR and RBC filling percentage was observed. Aerobic exercise did not induce changes in PBR or RBC filling percentage, suggesting that the endothelium of microvasculature facilitates efficient perfusion in vessels reacting with an increased endothelial surface layer.
JOURNAL OF CLINICAL MONITORING AND COMPUTING
(2022)
Article
Biochemical Research Methods
Wei Wei, Andrea Cogliati, Cristina Canavesi
Summary: A new OCTA technique, URFA, was developed to extract high-resolution cutaneous vasculature, significantly improving the visualization and characterization of micro-capillaries in vivo, and reducing motion artifacts by nearly 50% on average.
BIOMEDICAL OPTICS EXPRESS
(2021)
Review
Cell Biology
Amy B. Schwartz, Obed A. Campos, Ernesto Criado-Hidalgo, Shu Chien, Juan C. del Alamo, Juan C. Lasheras, Yi-Ting Yeh
Summary: Leukocyte transendothelial migration is a critical process for innate immunity and inflammation, involving intricate steps of leukocytes passing through endothelial cell layers. Biomechanics plays a decisive role in this process, and recent technological advances enable the study of how cellular forces affect endothelial barrier integrity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Heon-Woo Lee, Yanying Xu, Liqun He, Woosoung Choi, David Gonzalez, Suk-Won Jin, Michael Simons
Summary: Venous endothelial cells are identified as the primary subtype responsible for the expansion of angiogenic vascular networks, arteriovenous malformations formation, and pathological angiogenesis. They proliferate, migrate against the blood flow, and differentiate into different types of endothelial cells during both physiological and pathological angiogenesis processes. This highlights the crucial role of venous endothelium in both normal development and disease pathogenesis.
Article
Biotechnology & Applied Microbiology
Andrea Weinzierl, Yves Harder, Daniel Schmauss, Michael D. Menger, Matthias W. Laschke
Summary: This study investigates the regenerative mechanisms of nanofat grafts in a murine model, finding that nanofat promotes tissue vascularization and the formation of new microvascular networks. These findings suggest that nanofat could be a versatile resource for tissue engineering and regenerative medicine.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Xiaowan Han, Tong Li, Tieshan Wang, Baofu Wang, Yang Li, Lei Wang, Ziwen Lu, Aiming Wu, Lisong Liu, Guozhong Pan, Mingjing Zhao
Summary: This study evaluated the correlation between different microparticle phenotypes and plaque burden, and investigated the role of microparticles in atherosclerosis. The results showed that CD11a(+) LMP and CD11a(+)/CD45(+) LMP may be potential biomarkers for predicting carotid atherosclerosis.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Endocrinology & Metabolism
Michael P. Dybala, Manami Hara
Summary: The pancreas is composed of two separate systems - the endocrine and exocrine pancreas. Treatment of diseases affecting either system may impact the overall pancreatic function, leading to different disciplines of medical professionals providing treatment. A new model suggests that islet blood flow is integrated with the surrounding exocrine capillary network, potentially offering new hypotheses for pancreatic diseases and their regulation of hormones and functions.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kirsten Jander, Jan Greulich, Stefanie Gonnissen, Niloofar Ale-Agha, Christine Goy, Philipp Jakobs, Sabrina Farrokh, Corina Marziano, Swapnil K. Sonkusare, Judith Haendeler, Joachim Altschmied
Summary: The transcription factor Grainyhead-like 3 (GRHL3) has essential functions in endothelial cells beyond the nucleus. Mutants of GRHL3 can retain the ability to inhibit endothelial cell apoptosis, suggesting that the core function of this protein may be related to NO production.
Review
Orthopedics
Anna Burger, Amelie Sattler, Lisanne Grunherz, Pietro Giovanoli, Nicole Lindenblatt, Ulrich Michael Rieger
Summary: The objective of this study was to assess the satisfaction rate among male patients undergoing subcutaneous mastectomy, with a focus on scar tissue formation and the appearance of the chest wall. The results showed no significant difference in satisfaction based on the surgical approach used, but the majority of patients preferred more scars and a flatter chest wall. Additionally, a BMI greater than 25 kg/m² was associated with a higher risk of complications.
JOURNAL OF PLASTIC SURGERY AND HAND SURGERY
(2023)
Article
Surgery
Lisanne Grunherz, Felix Angst, Carlotta Barbon, Heike Hulla, Epameinondas Gousopoulos, Semra Uyulmaz, Susanne Lehmann, Stephan Wagner, Pietro Giovanoli, Nicole Lindenblatt
Summary: This study translated the LYMPH-Q Upper Extremity Module from English to German and conducted a comprehensive validation. The German version of the survey was shown to be conceptually equivalent to the original English version and proved to be a reliable and valid patient-reported outcome measure for assessing the physical and psychological impairments in patients with upper extremity lymphedema.
JOURNAL OF VASCULAR SURGERY-VENOUS AND LYMPHATIC DISORDERS
(2022)
Article
Pharmacology & Pharmacy
Bong-Sung Kim, Shih-Heng Chen, Mauro Vasella, Marco Guidi, Epameinondas Gousopoulos, Nicole Lindenblatt, Huang-Kai Kao
Summary: This study investigates the in vivo fate of mechanically processed stromal vascular fraction (mSVF) for regenerative purposes. By establishing an arteriovenous (AV) shunt model in rats, the study shows that vascularized mSVF can proliferate and express adipocyte-specific markers.
Article
Surgery
Nicole Lindenblatt, Lisanne Gruenherz, Anna Wang, Epameinondas Gousopoulos, Carlotta Barbon, Semra Uyulmaz, Pietro Giovanoli
Summary: This study reports the first-in-human use of the Symani surgical system for lymphatic reconstruction. Robot-assisted lympho-venous and arterial anastomosis were performed with high accuracy and safety. Further research is needed to explore the true potential of robotics in microsurgery.
PLASTIC AND RECONSTRUCTIVE SURGERY-GLOBAL OPEN
(2022)
Review
Surgery
Semra Uyulmaz, Inga Swantje Besmens, Holger Jan Klein, Pietro Giovanoli, Nicole Lindenblatt
Summary: The free split anterolateral thigh flap is an ideal option for complex tissue defects, with low complication rates and versatility in pharyngeal reconstruction.
INDIAN JOURNAL OF SURGERY
(2023)
Article
Genetics & Heredity
Carlotta Barbon, Lisanne Grunherz, Riccardo Schweizer, Nicole Lindenblatt, Pietro Giovanoli
Summary: The Urofacial or Ochoa Syndrome is a rare congenital disorder that affects both the urinary system and facial expression. This study reports the successful use of Botulinum toxin to address the impaired facial mimicry in a patient with this syndrome.
AMERICAN JOURNAL OF MEDICAL GENETICS PART A
(2023)
Article
Critical Care Medicine
Lisanne Grunherz, Ramona Michienzi, Claudine Schaller, Daniel Rittirsch, Semra Uyulmaz, Bong - Sung Kim, Pietro Giovanoli, Nicole Lindenblatt
Summary: It has been shown that Nexobrid(R) is a safe and effective method for debriding deep burns and reducing the need for surgical escharotomy. However, there is limited evidence for its use in larger body areas. This retrospective study found that the use of Nexobrid(R) for preventive decompression was less common but had a lower mortality rate compared to surgical escharotomy.
Article
Surgery
Inga S. Besmens, Charlotte Shahrdar, Duveken B. Y. Fontein, Sophie Knipper, Pietro Giovanoli, Nicole Lindenblatt
Summary: This study analyzed the factors affecting the outcome and need for revision rhinoplasty in patients who underwent closed reduction for nasal bone fracture. The results showed that patients with an additional septum fracture were more likely to require revision rhinoplasty. The risk of needing open revision rhinoplasty after fracture healing increased for patients complaining of airway obstruction at the time of cast removal after closed reduction.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2023)
Article
Surgery
Anna Burger, Marcus Kiehlmann, Lisanne Gruenherz, Epameinondas Gousopoulos, Michael Sohn, Nicole Lindenblatt, Pietro Giovanoli, Ulrich Michael Rieger
Summary: This study aimed to evaluate the use of a dermal template called MatriDerm (R) in reducing complications and hospital stay in phalloplasty surgeries. The use of MatriDerm (R) and split-thickness skin graft significantly reduced the rate of complications compared to full-thickness skin graft. The forearm and groin areas were both affected by complications in the full-thickness skin graft group.
INDIAN JOURNAL OF SURGERY
(2023)
Article
Surgery
Anna Wang, Lisanne Grunherz, Ilaria V. De Martini, Mauro Vasella, Pietro Giovanoli, Nicole Lindenblatt
Summary: This study compared the outcomes and satisfaction of autologous lipofilling performed during active and stable phases of PHA and LS. The results showed similar treatment outcomes in both groups, with social factors having the greatest negative impact on quality of life.
Article
Surgery
Lisanne Grunherz, Carlotta Barbon, Epameinondas Gousopoulos, Semra Uyulmaz, Pietro Giovanoli, Nicole Lindenblatt
Summary: Patients with upper and lower limb lymphedema can experience various physical and psychological symptoms that reduce their quality of life. Lymphatic reconstructive surgery has been proven to be beneficial for these patients, but solely relying on volume reduction measurements may not accurately evaluate postoperative outcomes. This study found that patients reported significant improvements in various aspects, particularly in physical function, symptoms, and psychological well-being. There was no correlation between the extent of volume reduction and improvement in quality of life, highlighting the importance of using standardized patient-reported outcome measures to evaluate the benefits of lymphatic reconstructive surgery.
PLASTIC AND RECONSTRUCTIVE SURGERY-GLOBAL OPEN
(2023)
Article
Engineering, Biomedical
Adam Wahlsten, Alberto Stracuzzi, Ines Luchtefeld, Gaetana Restivo, Nicole Lindenblatt, Costanza Giampietro, Alexander E. Ehret, Edoardo Mazza
Summary: The mechanical properties of the skin have been studied at both tissue and cellular length scales. The tension-compression nonlinearity of the collagen fiber network and the properties of the ground matrix contribute to the elasticity of the skin. Microscale indentation measurements do not engage the fiber network, and therefore cannot accurately predict the behavior of dermal fibroblasts.
ACTA BIOMATERIALIA
(2023)
Article
Surgery
Andrea Weinzierl, Carlotta Barbon, Epameinondas Gousopoulos, Donata von Reibnitz, Pietro Giovanoli, Lisanne Grunherz, Nicole Lindenblatt
Summary: Micro- and supermicrosurgeries have become standard techniques for lymphatic reconstruction. Robotic-assisted surgery is a new approach that allows for better accessibility of deep anatomical regions, although it sacrifices operating speed. In a case series of 8 patients, robot-assisted lymphatic microsurgery showed clear benefits in surgeon positioning and access to recipient vessels near the thoracic wall.
Article
Surgery
Lisanne Gruenherz, Epameinondas Gousopoulos, Carlotta Barbon, Semra Uyulmaz, Pietro Giovanoli, Nicole Lindenblatt
Summary: In recent years, surgical robotic systems specifically designed for microsurgery have expanded the use of robotic-assisted surgery in plastic reconstructive procedures. Currently, there are two microsurgical robotic systems available for reconstructive plastic surgery. These systems incorporate tremor reduction and motion scaling technologies to enhance surgical precision and dexterity. At the University Hospital Zurich, the Symani Surgical System has been successfully used for various microsurgical and supermicrosurgical procedures, including autologous breast reconstruction, nerve transfer, and reconstructive lymphatic surgery. Despite technical challenges like the lack of haptic feedback, the benefits outweigh the drawbacks for trained and skilled microsurgeons, offering smaller incisions and improved surgical precision for deep anatomical structures.
Article
Medicine, Research & Experimental
Pablo Pfister, Pedro David Wendel-Garcia, Isabelle Meneau, Mauro Vasella, Jennifer Ashley Watson, Philipp Buehler, Daniel Rittirsch, Nicole Lindenblatt, Bong-Sung Kim
Summary: Burn wounds present challenges in medical treatment, with the use of amniotic membranes as a biological dressing showing promise for faster healing and improved scar quality. This study aims to investigate the efficacy of using human amniotic membranes in treating burn wounds, comparing them to allogenic skin grafts in a randomized-controlled trial involving 30 patients.
CONTEMPORARY CLINICAL TRIALS COMMUNICATIONS
(2023)
