4.7 Article

Electrochemical genosensor based on carboxylated graphene for detection of water-borne pathogen

Journal

SENSORS AND ACTUATORS B-CHEMICAL
Volume 275, Issue -, Pages 312-321

Publisher

ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.07.055

Keywords

E.coli; Graphene nanoflakes; Biosensors; Reduced graphene oxide; Electrophoretic deposition

Funding

  1. UGC, India
  2. CSIR, India
  3. Department of Science and Technology, India under DST-INSPIRE Faculty project [DST/INSPIRE/04/2015/000932]

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This work reports the application of newly synthesized carboxylated graphene nanoflakes (Cx-Gnfs). The Cx-Gnfs were synthesized by wet chemical method in sulfuric acid/nitric acid mixture and was further electrophoretically deposited on indium fin oxide (ITO) coated glass substrates using Mg(2+ )ions which provides an overall charge to the materials for deposition onto the anode. The materials were characterized using SEM, TEM, contact angle, UV-vis spectroscopy, FT-IR, XRD and electrochemically characterized by cyclic voltammetry, chronocoulometry and electrochemical impedance spectroscopy. The sensitive quantitative determination of nucleic acid Escherichia coli O157: H7 (E. coil) has been achieved using Cx-Gnfs and r-GO as the sensing layer using electrochemical impedance spectroscopy. The electrochemical results reveal that the Cx-Gnfs based genosensor exhibits a linear response to complementary DNA (10(-6) M to 10(-17) M) with a detection limit of 1 x 10(-17) M while the rGO based genosensor shows a detection limit of 1 x 10(-15) M. Under optimal conditions, this Cx-Gnfs based genosensor was found to retain about 85% of its initial activity after being used for 6 times.

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