Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 194, Issue -, Pages 71-78Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.12.074
Keywords
Silver nanocubes; Chitosan; Horseradish peroxidase; Glucose oxidase; Biosensor
Funding
- National Natural Science Foundation of China [30800247, 20805043]
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This paper reported the fabrication of an amperometric glucose biosensor based on silver nanocubes (AgNCs) and horseradish peroxidase (HRP)-chitosan (CS)-glucose oxidase (Gox) bienzymatic film. The fabrication process was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and atomic force microscopy (AFM). Cyclic voltammetry (CV) and amperometry measurements were used to study and optimize the performance of the resulting peroxide biosensor. Under the optimal conditions, the sensor exhibits good electrocatalytic activity toward the oxidation of glucose, and this enables the determination of glucose in the 10 mu M to 1.5 mM concentration range, with a detection limit at 0.6977 mu M (at an S/N of 3). The response time is less than 5 s. The bienzymatic electrode presented a number of attractive features such as high sensitivity, low detection limit, fast response, and good stability, making this Gox-CS-HRP/AgNCs-CS nanocomposite film a promising candidate for glucose sensors. (C) 2014 Elsevier B. V. All rights reserved.
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