Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 192, Issue -, Pages 36-41Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.10.002
Keywords
Microfluidics; Single cell analysis; Hydrodynamic trapping; PALM imaging; Single molecule localisation microscopy
Funding
- Wellcome Trust
- Royal Society
- Medical Research Council [MR/K015850/1] Funding Source: researchfish
- MRC [MR/K015850/1] Funding Source: UKRI
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We describe a microfluidic device designed specifically for the reversible immobilisation of Schizosaccharomyces pombe (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within living cells with a modal accuracy of >25 nm in the lateral dimensions. The novel flow design captures and holds cells in a well-defined array with minimal effect on the normal growth kinetics. Cells are held over several hours and can continue to grow and divide within the device during fluorescence imaging. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
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