Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 177, Issue -, Pages 818-825Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.12.004
Keywords
Thrombin; Nanoparticles; Aptamer; Biosensor; UV-vis
Funding
- National Natural Science Foundation of China [20903008, 20973019, 50725208]
- Key Project Funding of NSFC [20973019, 50831001]
- NSFC for Outstanding Young Talents [507252008, 10825419]
- National 973 Program [2009CB623700]
- Students Research Training Program (SRTP)
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Herein, we report a sensitive and selective detection method for thrombin based upon oligonucleotide-functionalized gold nanoparticle probes that can combine with the thrombins by van der Waals force, where the thrombin molecule acts as a bridge to link the monodispersed aptamer-modified gold nanoparticles together, since thrombin includes two binding sites for the aptamer. This leads to the visible color changes from wine-red to blue-purple, which relies upon the distance-dependent optical properties of gold nanoparticles. The concentration can be determined by monitoring with naked eye or a UV-vis spectrometer. In addition, this assay does not utilize any organic cosolvents, heavy metal ion, enzymatic reactions, light-sensitive dye molecules, lengthy protocols, or sophisticated instrumentation, thereby overcoming some limitations of more conventional methods. Moreover, thrombin at concentrations as low as 5 pM (1.679 ng/mL) can be detected in this assay with a calorimetric format. (c) 2012 Elsevier B.V. All rights reserved.
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