Article
Chemistry, Analytical
Clarice E. Froehlich, Jiayi He, Christy L. Haynes
Summary: Investigating the interactions between small, charged molecules and aptamers using surface plasmon resonance (SPR) is challenging due to low small molecule response and nonspecific electrostatic interactions. However, aptamers are increasingly used in sensors for small molecule detection, and a simple, direct SPR assay would greatly benefit sensor development. This study presents a method using streptavidin-biotin capture and a high-ionic-strength buffer to characterize the interaction between serotonin and an aptamer with SPR. Other methods, such as immobilization and thiol-coupling, were also investigated for comparison.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Siddharth Sourabh Sahu, Christiane Stiller, Elizabeth Paz Gomero, Abel Nagy, Amelie Eriksson Karlstrom, Jan Linnros, Apurba Dev
Summary: An electrical immuno-sandwich assay utilizing an electrokinetic-based streaming current method is proposed, demonstrating improved target selectivity and linear concentration dependent response. The study explores the role of electric charge in detection sensitivity and the potential for further improvement, showcasing a clear enhancement of detection signal with increasing DNA lengths conjugated to the detection probe.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Tomas Springer, Zdenek Krejcik, Jiri Homola
Summary: A novel and extremely sensitive label-free optical biosensor-based assay for the detection of miRNAs has been proposed, which enhances specific sensor response while suppressing non-specific binding interference. The assay has been successfully applied to detect miRNAs related to myelodysplastic syndromes in blood plasma, achieving a low detection limit and enabling multiplexed miRNA detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Jing Zheng, Hui Zhao, Guobao Ning, Weijie Sun, Li Wang, Huan Liang, Hanbin Xu, Chaoyong He, Can-Peng Li
Summary: This study presented a novel affinity peptide-antibody sandwich electrochemical strategy for the ultrasensitive detection of prostate-specific antigen (PSA). The fabricated sensor exhibited excellent selectivity, ideal repeatability, and good stability for PSA detection in real samples, showing a low detection limit and a linear response range. The porous structure of the COF used in the sensor also contributed to the enrichment of MB molecules and increased the sensitivity of the biosensor.
Article
Chemistry, Analytical
Yuchen Bai, Jie Fei, Weilin Wu, Leina Dou, Minggang Liu, Shibei Shao, Wenbo Yu, Kai Wen, Jianzhong Shen, Zhanhui Wang
Summary: This study determined the minimum distance between two epitopes in sandwich immunoassays for small molecules. The results provide a theoretical basis for hapten design, antibody discovery and development, and the establishment of sandwich immunoassays.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Clarice E. Froehlich, Jiayi He, Christy L. Haynes
Summary: Investigating the interactions between small, charged molecules and aptamers using surface plasmon resonance (SPR) is challenging due to the low response of small molecules and nonspecific electrostatic interactions. However, aptamers are increasingly used in sensors for small molecule detection. This study develops a method to characterize the interaction between the charged small molecule serotonin and an aptamer using streptavidin-biotin capture and a high-ionic-strength buffer. These techniques provide insight into working with small molecules and offer the potential for improving SPR sensors.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Chenglong Sun, Yuru Shen, Yu Zhang, Yun Du, Yueyi Peng, Qingji Xie
Summary: The article introduces a CdS/AuNPs/NiO/FTO electrode with a Z-scheme heterojunction, which enables detection of specific chemicals through immobilization. This method has high sensitivity and a low detection limit, and exhibits satisfactory performance in the detection of real samples.
Article
Multidisciplinary Sciences
Jianye Sui, Neeru Gandotra, Pengfei Xie, Zhongtian Lin, Curt Scharfe, Mehdi Javanmard
Summary: The study introduces a new method for DNA detection by integrating paramagnetic beads with DNA fragments and using a microfluidic chip to measure Impedance Peak Response. The method shows high sensitivity and selectivity in detecting DNA quantities, as well as the ability to quickly analyze DNA fragment length and quantity.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Hyeong-Min Kim, Hyo-Jun Kim, Jae-Hyoung Park, Seung-Ki Lee
Summary: This study combines sandwich immunoassay with fiber-optic localized surface plasmon resonance (FO LSPR) for real-time and high-sensitivity analysis of low-concentration targets. By using second antibody-second gold nanoparticle conjugates, the detection limit is significantly enhanced and has good selectivity. The practical applicability of this method in detecting patient serum is confirmed and compared with a commercial assay kit.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Yinqi Tian, Lin Yuan, Min Zhang, Youfen He, Xucong Lin
Summary: A sensitive colorimetric immunoassay was developed for trace detection of the marine toxin okadaic acid (OA) using Au@Pt nanoparticles and horseradish peroxidase (HRP). The method showed improved sensitivity and good recovery rates in shellfish samples.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Paras H. Kundalia, Lucia Pazitna, Kristina Kianickova, Eduard Jane, Lenka Lorencova, Jaroslav Katrlik
Summary: Protein-carbohydrate interactions are essential in biology and microarrays have become a popular high-throughput method to study these interactions. The precise recognition of target glycan ligands among others is crucial for microarray analysis. This article investigates the influence of various external factors on protein-carbohydrate interactions and proposes a 4D approach to minimize the effects of these factors on glycomics microarray analysis and streamline cross-platform comparisons. This work will optimize microarray analysis for glycomics and enhance the development of this technology.
Article
Engineering, Biomedical
Jorge Manrique Castro, Frank Sommerhage, Rishika Khanna, Andre Childs, David DeRoo, Swaminathan Rajaraman
Summary: In recent years, the biomedical scientific community has been focused on developing high-throughput devices for the reliable and rapid detection of multiple virus strains or microparticles simultaneously. The complexity lies in the rapid prototyping of new devices and wireless detection of small particles and viruses. By simplifying microfluidics microfabrication and using affordable materials and makerspace tools, an affordable solution to high-throughput devices and detection technologies can be provided. We present the development of a wireless, standalone device and disposable microfluidic chips that can rapidly generate parallel readouts for selected virus variants from nasal or saliva samples.
BIOMEDICAL MICRODEVICES
(2023)
Article
Chemistry, Analytical
Chaiyong Wu, Dengfeng Li, Qianli Jiang, Ning Gan
Summary: A paper-based fluorescent phage biosensor was developed for the rapid and specific detection of virulent E. coli O157:H7 strain in food samples. The biosensor utilized a novel fluorescent probe and a micro-porous membrane filter to retain the targeted complex on a paper chip. The detection limit was as low as 50 CFU/mL, showing potential application for point-of-care testing of virulent E. coli in foods.
Article
Biochemistry & Molecular Biology
Chen-Chun Chen, Han-Wen Huang, Bo-Rui Chen, Chi-Huey Wong
Summary: A quantitative analytical method using mass spectrometry and enzymatic reactions has been developed for the detection of alpha-fetoprotein in hepatocellular carcinoma.
RSC CHEMICAL BIOLOGY
(2023)
Proceedings Paper
Materials Science, Multidisciplinary
Pankaj Kumar Singh, Monika Goyal
Summary: This study integrates three different cancer markers into a multiplex quantization detection system using semiconductor nano quantum dots and microfluidic bio-sensors. By utilizing a sandwich-type immuno-test approach, a nano-bio-chip has been developed, which can detect cancer markers more effectively compared to standard methods.
MATERIALS TODAY-PROCEEDINGS
(2021)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)