Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 182, Issue -, Pages 668-674Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.03.070
Keywords
Aptamer; Electrochemical detection; Magnetic particles; Monovalent labeling; Small molecule; Tobramycin
Funding
- Spanish Government
- Ramon y Cajal
- European Regional Development Fund
- [CTQ2008-02429]
Ask authors/readers for more resources
The design and performance of an inhibition assay for the electrochemical detection of tobramycin is reported. This platform uses a monovalent system for introducing the enzyme conjugate on a tagged-aptamer that specifically recognizes the aminoglycoside antibiotic tobramycin. Compared with multivalent systems such as biotin-streptavidin, the sensitivity is greatly improved reducing the limit of detection from 5 mu M to 0.1 mu M in aqueous solution. This labeling strategy along with the use of a nuclease-resistant RNA aptamer allowed the determination of tobramycin in pre-treated human serum samples within the therapeutic range, using magnetic microparticles as a solid support for the aptassay, and differential pulse voltammetry as detection technique. (C) 2013 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available