Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 157, Issue 1, Pages 211-215Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2011.03.051
Keywords
Urea biosensor; Potentiometry; Electrochemical polymerization; Urease; Poly-o-phenylenediamine; Buffering capability
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A simple and novel potentiometric biosensor for urea detection was prepared by employing an electrosynthesized polymer with buffering capability. It was obtained by deposition of a weighed amount of urease (Ur) at a glassy carbon (GC) electrode followed by immobilization by an electrosynthesized poly-o-phenylenediamine (PPD) film. An unconventional upside-down (UD) geometry was employed for the electrochemical cell. The response of GC/Ur/PPD sensor is linear with urea concentration in the range 10 mu M to 1 mM (15 mV/mM, R-2 = 0.9999) due to buffering capability of PPD film, which represents a novel role of electrosynthesized polymers in their application to biosensors. At higher concentrations, the more common Nernstian response (28 mV/decade, R-2 = 0.9987) is observed. The sensor exhibits a sufficient sensitivity for practical determinations, rapid response and long term stability. (C) 2011 Elsevier B.V. All rights reserved.
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