Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 160, Issue 1, Pages 129-138Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2011.07.023
Keywords
Alizarin Red S; Boronic acid; Glucose; Micelle; Fluorescence sensor
Funding
- Higher Education Research Promotion
- National Research University Project of Thailand
- Office of the Higher Education Commission, through the Advanced Functional Materials Cluster of Khon Kaen University
- Thailand Research Fund [RTA5380003]
- Center for Innovation in Chemistry (PERCH-CIC), Commission on Higher Education, Ministry of Education
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The sensitivity of glucose sensors fabricated from alizarin-boronic acid adducts was improved by an assistance of surfactants. Basically, Alizarin Red S (ARS) is not a fluorescence active compound. However, the fluorescence emission can be observed when forming an adduct with 2-formylphenyl boronic acid (2-FPBA). Using displacement strategy, the fluorescence intensity of the ARS/2-FPBA adduct decreased as a linear function of the glucose concentration. A simple and sensitive method was developed by incorporating the ARS/2-FPBA adduct in the hydrophobic core of micelles. Various parameters that could possibly affect the fluorescence quenching ability including solution pH, concentration of 2-FPBA, types of surfactants and concentrations of surfactants were investigated. The optimum condition for the determination of glucose by the proposed assay was 2 mM cetyltrimethylammonium bromide (CTAB) in 0.05 M phosphate buffer pH 7.0. The fluorescence intensity of the ARS/2-FPBA adduct in the presence of CTAB was remarkably 13 times higher than that in the buffer solution. Moreover, the linear working concentration range was found to be 1.2-80 mM, and the calibration sensitivity was 14 times higher than that from the system containing only buffer solution. (C) 2011 Elsevier B.V. All rights reserved.
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