Article
Biochemical Research Methods
Xiaoyong Chen, Pin Xu, Wenwei Lin, Jin Jiang, Hang Qu, Xuehao Hu, Jinghua Sun, Yukun Cui
Summary: This study proposes a plasmonic titled fiber Bragg grating biosensor for the detection of breast cancer cells. Experimental results demonstrate that the biosensor has good detection performance, capable of detecting low concentrations of breast cancer cells in a short period of time. The sensor has a simple structure, easy manufacturing process, making it a promising choice for future cancer cell detection.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Physics, Applied
Weiqi He, Weina Zhang, Jiantian Zhang, Peng Yu, Pu Liu, Guowei Yang, Hongxiang Lei
Summary: Utilizing flexible dielectric material polylactic acid (PLA) doped with high refractive index quantum dots (QDs) as the substrate, a nanowire RI sensor capable of high sensitivity and the highest FOM under white light source is fabricated, showing great potential for biological applications.
APPLIED PHYSICS LETTERS
(2021)
Article
Chemistry, Analytical
Shimeng Chen, Shuwen Chu, Yongxin Song, Haojun Wu, Yun Liu, Wei Peng
Summary: Binding behaviors of proteins play a crucial role in biochemistry applications. This paper presents a label-free sensing approach based on a near-infrared surface plasmon resonance sensing chip modified with a graphene-gold surface architecture. The method demonstrates enhanced sensitivity and detection capability through the use of graphene and successfully detects human immunoglobulin G.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Shimeng Chen, Yun Liu, Qingxu Yu, Wei Peng
Summary: A novel SPR biosensing approach with low LOD, high sensitivity, and extensive applicability was introduced in this study. By optimizing the SPR sensor structure and employing a self-referencing sensing strategy, the low LOD of 0.2 fM was achieved.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Zhitao Lin, Yiqing Shu, Weicheng Chen, Yang Zhao, Jianqing Li
Summary: PtSe2 is used to modify traditional SPR biosensors, and a metal-Si-metal waveguide structure is proposed to further improve the performance of the biosensor. The highest sensitivity was achieved with the BK7-Au-Si-Au-PtSe2 (2 nm) biosensor structure.
Article
Chemistry, Multidisciplinary
Mengdi Lu, Wei Peng, Ming Lin, Fang Wang, Yang Zhang
Summary: A self-assembled templating technique using block copolymer layer was proposed for LSPR sensors, resulting in a high uniformity and density of gold nanoparticle monolayer. The performance in LSPR resonance wavelength and refractive index sensitivity significantly improved compared to conventional techniques, with a notable decrease in detection limit.
Article
Optics
Kaijun Liu, Binbin Luo, Decao Wu, Hong Gu, Xiangwen Yang
Summary: This study simulated the cladding mode characteristics of an excessively tilted fiber grating coated with gold nanoshells. It was found that the localized surface plasmon resonance effect can enhance the refractive index sensitivity of the fiber grating.
Article
Biophysics
Tianping Xu, Zhaoxin Geng
Summary: Due to the lower sensitivity of LSPR biosensors compared to other sensors, researchers have explored various methods to improve performance, including structural, material, and interface modifications. These efforts are expected to expand the applications of LSPR biosensing technology.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Multidisciplinary Sciences
Fuhito Nakagawa, Marin Kikkawa, Sisi Chen, Yasuomi Miyashita, Norie Hamaguchi-Suzuki, Minami Shibuya, Soichi Yamashita, Lisa Nagase, Satoshi Yasuda, Mitsunori Shiroishi, Toshiya Senda, Keisuke Ito, Takeshi Murata, Satoshi Ogasawara
Summary: Nanodisc technology is a powerful tool for analyzing molecular interactions of membrane proteins. In this study, a specific antibody called biND5 was generated for the analysis of nanodiscs. The antibody showed high affinity for various types of nanodiscs and specifically recognized a certain structure within the membrane scaffold protein. Use of this antibody facilitated the analysis of molecular interaction kinetics between adenosine A(2a) receptor nanodiscs and a small molecule antagonist.
SCIENTIFIC REPORTS
(2023)
Review
Chemistry, Analytical
Songfeng Huang, Jiajie Chen, Teliang Zhang, Xiaoqi Dai, Xueliang Wang, Jianxing Zhou, Weifu Kong, Qian Liu, Junle Qu, Yonghong Shao
Summary: Surface plasmon resonance microscopy (SPRM) is a versatile technique for biosensing and imaging that enables high-sensitivity, label-free, real-time characterization. This report provides an overview of the research progress in SPRM for plasma imaging and sensing, summarizing the technological advances and important applications. The combination of various new techniques with SPRM is emphasized.
Article
Polymer Science
Yi-Jung Lu, Han-Yun Hsieh, Wen-Chang Kuo, Pei-Kuen Wei, Horn-Jiunn Sheen, Hidetoshi Tahara, Te-Wei Chiu, Yu-Jui Fan
Summary: The study proposed a multiplex detection system by integrating LSPR sensing array and microfluidic channels, proving successful in detecting different targets in parallel channels.
Article
Chemistry, Analytical
Shimeng Chen, Haojun Wu, Yongxin Song, Wei Peng, Yun Liu
Summary: This paper presents a fiber-optic surface plasmon resonance (FO-SPR) biosensor utilizing image processing and back propagation neural network to accurately monitor refractive index changes in biochemical reactions. Experimental results show the effectiveness and superiority of this sensing system for practical applications in standardized biochemical sensing.
Article
Chemistry, Analytical
Mengjiao Ren, Chengpeng Ji, Xueyan Tang, Haishan Tian, Leyong Jiang, Xiaoyu Dai, Xinghua Wu, Yuanjiang Xiang
Summary: In this paper, a sensitivity-tunable terahertz (THz) liquid/gas biosensor based on a coupling prism-three-dimensional Dirac semimetal (3D DSM) multilayer structure is studied. The high sensitivity of the biosensor is attributed to the sharp reflected peak caused by surface plasmon resonance (SPR) mode. The reflectance of the biosensor can be modulated by the Fermi energy of 3D DSM, resulting in tunable sensitivity. After parameter optimization, the sensitivity of the liquid biosensor reaches over 100°/RIU. This simple structure provides a reference idea for achieving high sensitivity and tunable biosensor devices.
Article
Biophysics
Tomas Springer, Zdenek Krejcik, Jiri Homola
Summary: A novel and extremely sensitive label-free optical biosensor-based assay for the detection of miRNAs has been proposed, which enhances specific sensor response while suppressing non-specific binding interference. The assay has been successfully applied to detect miRNAs related to myelodysplastic syndromes in blood plasma, achieving a low detection limit and enabling multiplexed miRNA detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Yichao Xu, Meng Xiong, Hui Yan
Summary: A low-cost, portable, and reusable optical fiber-based LSPR biosensor for detecting ZEN was developed in this study, showing good sensitivity, selectivity, and potential for on-site ZEN monitoring.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Zdenka Kristofikova, Tomas Springer, Erika Gedeonova, Adela Hofmannova, Jan Ricny, Lenka Hromadkova, Martin Vyhnalek, Jan Laczo, Tomas Nikolai, Jakub Hort, Tomas Petrasek, Ales Stuchlik, Karel Vales, Jan Klaschka, Jiri Homola
NEUROCHEMICAL RESEARCH
(2020)
Editorial Material
Biochemical Research Methods
Antje J. Baeumner, Guenter Gauglitz, Jiri Homola
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Tomas Springer, Erika Hemmerova, Giusy Finocchiaro, Zdenka Kristofikova, Martin Vyhnalek, Jiri Homola
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Biochemistry & Molecular Biology
Erika Hemmerova, Tomas Springer, Zdenka Kristofikova, Jiri Homola
Article
Biochemistry & Molecular Biology
Erika Hemmerova, Tomas Springer, Zdenka Kristofikova, Jiri Homola
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Chemistry, Physical
Hana Sipova-Jungova, Ludmila Jurgova, Erika Hemmerova, Jiri Homola
Summary: Functional materials with organic coatings on inorganic substrates are considered potential platforms for various applications, leading to the investigation of their interactions with the micro-environment becoming a key research direction in surface science. This study focused on the interactions of Tris buffer with self-assembled monolayers (SAMs) of alkanethiols and short DNAs using a surface plasmon resonance (SPR) biosensor, showing that the interaction is a complex multiphasic process. The study also revealed how the interaction of Tris with immobilized DNA molecules can affect the determination of surface concentrations and hybridization efficiencies.
APPLIED SURFACE SCIENCE
(2021)
Article
Biophysics
Tomas Springer, Zdenek Krejcik, Jiri Homola
Summary: A novel and extremely sensitive label-free optical biosensor-based assay for the detection of miRNAs has been proposed, which enhances specific sensor response while suppressing non-specific binding interference. The assay has been successfully applied to detect miRNAs related to myelodysplastic syndromes in blood plasma, achieving a low detection limit and enabling multiplexed miRNA detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Nanoscience & Nanotechnology
Hatice Altug, Sang-Hyun Oh, Stefan A. Maier, Jiri Homola
Summary: This Review presents the progresses in label-free nanophotonic biosensors based on photonic or dielectric surfaces and metasurfaces, and discusses their challenges and benefits in the fields of public health, well-being, and biosafety. Nanophotonic devices can control light and enhance light-matter interactions at subwavelength scales, offering exciting prospects for biosensing applications.
NATURE NANOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Jiri Slaby, Marketa Bockova, Jiri Homola
Summary: A new optical biosensor based on gold nanostripes array excited surface plasmons is presented, with theoretical analysis showing its improved detection sensitivity compared to conventional surface plasmon resonance biosensors. Experimental validation of the theoretical model demonstrates high refractive index sensitivity and resolution of the nanoplasmonic sensor. Additionally, the biosensor successfully detects microRNA biomarkers at low concentrations, showcasing its potential for sensitive and specific biomolecular detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Anna Ligasova, Ivan Rosenberg, Marketa Bockova, Jiri Homola, Karel Koberna
Summary: This study introduces a novel approach for real-time measurement of uracil DNA glycosylase activity by immobilizing selected oligonucleotides on magnetic nanoparticles and utilizing Forster resonance energy transfer. The results demonstrate that this method provides more reliable data than using free oligonucleotides, and offers the possibility to investigate the relationship between uracil DNA glycosylase activity and the arrangement of oligonucleotide probes. Additionally, introducing a nick into oligonucleotides containing the target base (uracil) significantly decreases the activity of both bacterial glycosylase and glycosylases naturally present in nuclear lysates.
Article
Biophysics
Jiri Slaby, Jiri Homola
Summary: In this study, we analyze the performance of label-free optical biosensors in detecting analyte-induced refractive index changes. We propose an analytical model that relates the refractive index resolution to the parameters of the optical platform. Our findings demonstrate that the widely used figure of merit (FOM) is not suitable for guiding the design or predicting the performance of label-free optical biosensors. Therefore, we propose an extended definition of FOM to address its limitations. The validity of our approach is confirmed through numerical simulations and experiments, and we show that the analytical model not only predicts the performance of biosensors but also provides strategies for achieving optimal performance.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Polymer Science
Yu-Min Wang, Anna Kalosi, Yuriy Halahovets, Iryna Romanenko, Jiri Slaby, Jiri Homola, Jan Svoboda, Andres de los Santos Pereira, Ognen Pop-Georgievski
Summary: This study compared the anti-fouling properties of poly(HPMA) brushes prepared by chemisorption and surface-initiated polymerization methods, finding that the latter exhibited better anti-fouling performance due to higher grafting density.
Proceedings Paper
Nanoscience & Nanotechnology
Jan Bukacek, Jiri Homola
11TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2019)
(2020)
Article
Chemistry, Multidisciplinary
Daria Kotlarek, Stefan Fossati, Priyamvada Venugopalan, Nestor Gisbert Quilis, Jiri Slaby, Jiri Homola, Mederic Lequeux, Frederic Amiard, Marc Lamy de la Chapelle, Ulrich Jonas, Jakub Dostalek
Article
Biochemical Research Methods
N. Scott Lynn, Tomas Springer, Jiri Slaby, Barbora Spackova, Michaela Grafova, Maria Laura Ermini, Jiri Homola
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
