Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 137, Issue 1, Pages 230-234Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2008.10.011
Keywords
Hydrogen peroxide; Hemoglobin; Triblock copolymer; Direct electrochemistry; Biosensor
Funding
- National Natural Science Foundation of China [20773088]
- National Basic Research Program of China [2008CB617504]
- Shanghai Rising Star Program
- Shanghai Key Laboratory of Rare-earth Functional Materials [07dz22303]
- Shanghai Leading Academic Discipline [S30406]
- Shanghai Municipal Education Commission [06DZ012]
- [08QH14020]
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A new film of triblock copolymer EO(20)PO(70)EO(20) (P123) was used to incorporate hemoglobin (Hb) onto the surface of pyrolytic graphite (PG) electrode for biosensor fabrication. UV-vis spectra Suggested that Hb in the film Could keep its native secondary structure. A pair of stable, well-defined and quasi-reversible cyclic voltammetric peaks was observed with the formal potential of about -0.29V(vs. SCE)at the Hb/P123 film-modified electrode in pH 6.0 NaAc-HAc buffer solution, characteristic of heme Fe(III)/Fe(II) redox couple of Hb. The modified electrode exhibited good electrocatalytic activity to the reduction of hydrogen peroxide (H(2)O(2)). The linear concentration range of the unmediated H(2)O(2) biosensor was from 1.0 x 10(-6) to 5.0 x 10(-4) M with a detection limit of 5.0 x 10(-7) M. The apparent Michaelis-Menten constant of Hb in the P123 film was estimated to be 0.51 mM, showing its high affinity. The stability. reproducibility and selectivity of the biosensor were also evaluated. (C) 2008 Elsevier B.V. All rights reserved.
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