Article
Chemistry, Analytical
Huizhen Li, Yunhui Li, Cenlin Gui, Daqi Chen, Lanfang Chen, Le Luo, Guobao Huang, Yang Yuan, Rong He, Fan Xia, Jiahai Wang
Summary: In this study, it has been confirmed that bare glass nanopores can accurately measure various lengths of DNA duplexes without adding organic solvent and embedding polymer hydrogel. The measurement has a resolution of 200 base pairs and shows well-separated peak amplitudes of blockage currents. By utilizing this capability, amplicons from PCR amplification can be discriminated without fluorescent labeling. This method can also discriminate various pathogenic bacteria and viruses, including SARS-CoV-2 and its mutants, in clinical samples with high resolution. Furthermore, the bare glass nanopore can quantitatively measure PCR amplicons without the need for extra labeling, with a detection range from 0.75 aM to 7.5 pM and a detection limit of 7.5 aM.
Article
Nanoscience & Nanotechnology
Kyung Hoon Kim, MinHo Yang, Younseong Song, Chi Hyun Kim, Young Mee Jung, Nam-Ho Bae, Sung-Jin Chang, Seok Jae Lee, Yong Tae Kim, Bong Gill Choi, Kyoung G. Lee
Summary: The development of a three-dimensional nanoweb film has enabled efficient capture of bacterial pathogens through hand-touching, demonstrating high capture efficiency and sensitivity. This unique nanostructure holds great potential for applications in capturing and detecting bacteria.
Article
Medicine, General & Internal
Jeong-Hwa Kim, Jae-Woon Oh, Young Lee, Jeong-Ho Yun, Seong-Ho Choi, Dong-Woon Lee
Summary: This study found that detecting 18 bacterial species in mouth-rinse samples can be used to diagnose periodontitis, with Treponema denticola, Prevotella nigrescens, and Streptococcus mitis significantly associated with severe periodontitis. Further research is needed to assess the potential of oral-rinsing solutions in indicating oral infection risk and improving oral hygiene.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Yan Zhang, Xianzhi Hu, Qingjiang Wang, Yi Zhang
Summary: Pathogenic bacteria pose a global threat to public health, and the development of rapid and highly sensitive detection strategies is urgently needed. Microchips, especially microfluidic devices, offer significant advantages for the detection of pathogenic bacteria. This review summarizes representative articles on microchip-based systems for analyzing pathogenic bacteria, providing a detailed overview of the techniques and discussing their advantages and disadvantages. The direction of development and prospects in the analysis of pathogenic bacteria are also examined.
CHINESE CHEMICAL LETTERS
(2022)
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Pediatrics
Hyun-Woo Lee, Seung-Beom Han, Jung-Woo Rhim
Summary: This study aimed to determine the subjects for bacterial multiplex polymerase chain reaction (mPCR) testing and to interpret the mPCR test results based on patients' clinical symptoms and diagnoses. The study found that positive mPCR test results were significantly associated with older age, diagnosis of acute gastroenteritis, presence of hematochezia, and absence of cough. Additionally, the identification of invasive pathogens in patients with positive mPCR test results was significantly associated with the diagnosis of acute gastroenteritis, presence of fever and diarrhea, and higher C-reactive protein levels. Therefore, selective bacterial mPCR testing should be performed based on the patients' clinical symptoms and diagnoses.
Article
Microbiology
Maria Daniela Silva, Antonio Lima, Nuno Marcal, Luis Dias, Miguel Gama, Sanna Sillankorva
Summary: Understanding the bacterial causes of otitis media is crucial for effective treatment. This study analyzed samples from 49 children with otitis media in Portugal, finding a correlation between bacteria in the middle ear fluid and nasopharynx. The most commonly identified species were Moraxella catarrhalis and Streptococcus pneumoniae, but Haemophilus influenzae was more prevalent in the middle ear fluid. The study also revealed the importance of polymicrobial infections in otitis media.
Article
Biochemical Research Methods
Koen J. van Aerde, Aniek Leegstraten, Corne H. van den Kieboom, Peter Merkus, Coosje Sintnicolaas, Sami O. Simons, Michiel van der Flier, Ronald de Groot, Marien de Jonge
Summary: In this study, a new sampling device called the modular breath sampler (MBS) was used to compare with conventional diagnostics in detecting pathogens in cystic fibrosis (CF) patients. The results showed differences in the pathogens detected, which may be due to the sampling from different compartments. Additionally, the MBS-based PCR diagnostics showed higher sensitivity than conventional culturing methods. Most of the parents/patients found the MBS easy to use and less burdensome than respiratory sampling.
JOURNAL OF BREATH RESEARCH
(2022)
Article
Multidisciplinary Sciences
Jae Jong Kim, Hyoung-Min Park, A. Young Kyoung, In Kyung Park, Si-Kyu Lim, Byoung Chul Park
Summary: Genetic mutations like SNP are common in genetic disorders and cancers, and PCR methods are widely used for SNP detection. The specifically designed PCR platform STexS can efficiently distinguish small amounts of SNP mixed in normal DNA, aiding in targeting potential SNPs or genetically mutated biomarkers in human clinical samples.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Alexander Nagy, Lenka Cernikova, Martina Stara
Summary: A new highly pathogenic avian influenza H5N1 genotype was detected in geese and ducks from a backyard farm in the Czech Republic. The Czech H5N1 virus retained the A/Eurasian_Wigeon/Netherlands/1/2020-like backbone but had an altered PB2 segment obtained from co-circulating low-pathogenic avian influenza viruses.
ARCHIVES OF VIROLOGY
(2022)
Article
Chemistry, Analytical
Lingyi Wu, Ting Zhou, Ru Huang
Summary: In this study, CRISPR/dCas9 was used as a universal ECL probe for sensitive and single-base-specific DNA detection. By changing the targeting region of the sgRNA, the probe can detect different DNA targets. This technology holds application potential in pathogen detection and gene typing.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Engineering, Chemical
Sahar Moradi, Reza Fouladi-Fard, Rahim Aali, Masoumeh Dolati, Saeed Shams, Mahdi Asadi-Ghalhari, Amir Hamta, Monireh Dehabadi
Summary: This study aimed to identify beta-lactam-resistance genes in municipal wastewater in Qom province and determine the effect of different treatment processes on reducing these pollutants. The results showed that conventional biological wastewater treatment processes not only have little potential to reduce antibiotic-resistance genes, but sometimes increase their presence. The study suggests that equipping treatment with advanced technologies will be efficient in combating bacterial resistance.
DESALINATION AND WATER TREATMENT
(2023)
Article
Immunology
Wei-Hsuan Lin, Han-Cheng Chiu, Kuan-Fu Chen, Kuo-Chien Tsao, Yi-Yin Chen, Ting-Hsuan Li, Yhu-Chering Huang, Yu-Chia Hsieh
Summary: In adult patients with CAP, bacterial pathogens accounted for 50% and viruses for 36.3%. Molecular testing could detect more pathogens than traditional culture techniques in diagnosing pneumonia. Co-infections with multiple pathogens, especially K. pneumoniae and human rhinovirus, were common in this study.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Immunology
Shunsuke Uno, Rei Goto, Kimiko Honda, Sho Uchida, Yoshifumi Uwamino, Ho Namkoong, Ayumi Yoshifuji, Kei Mikita, Yaoko Takano, Morio Matsumoto, Yuko Kitagawa, Naoki Hasegawa
Summary: Universal asymptomatic preoperative SARS-CoV-2 PCR screening is not cost-effective, and the cost-effectiveness mainly depends on the test-positive rate, the frequency of postoperative pulmonary complications, and screening costs.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)