Journal
SCIENTIFIC REPORTS
Volume 5, Issue -, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/srep15672
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Funding
- Consejeria de Salud, Fundacion Publica Andaluza Progreso y Salud, Junta de Andalucia [PI-0727-2010, PI-0085-2013]
- Consejeria de Economia, Innovacion y Ciencia [P10.CTS.6359]
- Ministerio de Economia y Competitividad, Instituto de Salud Carlos III
- Fondos FEDER [PI10/00871, PI13/00593]
- Fundacion Vencer el Cancer
- JDRF subsidy [17-2013-372]
- Instituto de Salud Carlos III [CP14/00105]
- FEDER
- Ministerio de Economia y Competitividad
- ALUSVI (Asociacion Lucha y Sonrie por la Vida de Pilas)
- local Andalusia association
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PAX4 is a key regulator of pancreatic islet development whilst in adult acute overexpression protects beta-cells against stress-induced apoptosis and stimulates proliferation. Nonetheless, sustained PAX4 expression promotes beta-cell dedifferentiation and hyperglycemia, mimicking beta-cell failure in diabetic patients. Herein, we study mechanisms that allow stringent PAX4 regulation endowing favorable beta-cell adaptation in response to changing environment without loss of identity. To this end, PAX4 expression was monitored using a mouse bearing the enhanced green fluorescent protein (GFP) and cre recombinase construct under the control of the islet specific pax4 promoter. GFP was detected in 30% of islet cells predominantly composed of PAX4-enriched beta-cells that responded to glucoseinduced insulin secretion. Lineage tracing demonstrated that all islet cells were derived from PAX4(+) progenitor cells but that GFP expression was confined to a subpopulation at birth which declined with age correlating with reduced replication. However, this GFP(+) subpopulation expanded during pregnancy, a state of active beta-cell replication. Accordingly, enhanced proliferation was exclusively detected in GFP(+) cells consistent with cell cycle genes being stimulated in PAX4-overexpressing islets. Under stress conditions, GFP(+) cells were more resistant to apoptosis than their GFP(-) counterparts. Our data suggest PAX4 defines an expandable beta-cell sub population within adult islets.
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