Article
Engineering, Environmental
Adrian Monteleone, Folker Wenzel, Heinz Langhals, Daniel Dietrich
Summary: The FLIM method was tested for identification and characterization of six types of plastics, showing significant differentiation in 94.55% of the comparisons. Results suggest that FLIM has the potential for sub-micrometer plastic characterization and allows for visual 3D-sectioning of samples, which could be important for identifying and characterizing plastics in tissue and environmental samples.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Adrian Monteleone, Weronika Schary, Folker Wenzel, Heinz Langhals, Daniel R. Dietrich
Summary: The study introduced a new method FLIM and phasor analysis for fast, label-free, and sensitive identification and differentiation of plastics, which represents a significant advancement in addressing the issue of plastic pollution.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Biochemistry & Molecular Biology
Andrew H. A. Clayton
Summary: The dynamics of condensed matter can be studied using the time-dependent Stokes shift of a fluorescent probe. The time-dependent spectral correlation function is described by spectral relaxation correlation times that characterize the relaxation processes around the excited-state probe. Phasor plot is a powerful tool to analyze time and frequency-domain data, providing spatial maps of spectral dynamics in complex structures like living cells. The analysis workflow also addresses the extraction of initial and relaxed generalized polarization and improved methods for model discrimination and parameter extraction in complex spectral relaxation phenomena.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Fang Zhou, Xin Wang, Guangxin Wang, Yanxia Zuo
Summary: With the increasing use and release of plastic products, microplastics have become a significant concern as they accumulate in the environment. This study proposes a fluorescence lifetime imaging technology for the rapid and accurate detection of microplastics, utilizing phasor analysis to obtain unique phasor fingerprints. The method allows for quick and intuitive identification of different microplastics and their distribution in mixed samples. The results show the potential applications of this method in identifying and analyzing microplastics in complex environments.
Article
Chemistry, Multidisciplinary
Chenshuang Zhang, Fangrui Lin, Yong Zhang, Haozhi Yang, Danying Lin, Jun He, Changrui Liao, Xiaoyu Weng, Liwei Liu, Yiping Wang, Bin Yu, Junle Qu
Summary: MSIM-SHG is a novel approach that combines second-harmonic generation (SHG) with multifocal structured illumination microscopy (MSIM) to achieve super-resolution imaging. It has wide applications in imaging material molecules and quantifying collagen fiber alignment, showing potential for clinical diagnosis and biological research.
Article
Chemistry, Physical
Wei Li, Yunpeng Ma, Tianyi Feng, Ziwan Du, Yixuan Liu, Sergei Kalinin, Jing-Feng Li, Qian Li
Summary: Understanding symmetry breaking in ferroelectric materials is crucial for manipulating their functional properties. We developed a spectral imaging method using optical second harmonic generation to investigate the mechanisms behind crystallographic symmetry breaking. By studying (K,Na)NbO3 single crystals, we demonstrated the method's ability to analyze domain variants and their thermal evolution across phase transitions. The method can be extended to other ferroelectric materials with potential enhancements.
JOURNAL OF MATERIOMICS
(2023)
Article
Multidisciplinary Sciences
Shingo Toyoda, Manfred Fiebig, Taka-hisa Arima, Yoshinori Tokura, Naoki Ogawa
Summary: Mirror symmetries play a crucial role in fundamental properties and conservation laws, with potential magnetoelectric cross-coupling when both spatial inversion and time reversal are broken. The giant nonreciprocal optical response observed in SHG of CuB2O4 is due to interference between magnetic-dipole and electric-dipole contributions, showing the potential for nonreciprocal nonlinear optical functionalities in multiferroics and magnetoelectrics. Resonantly enhanced magnetic-dipole transitions contribute significantly to the nonreciprocity, maximizing the effect despite the inherently weaker nature compared to electric-dipole transitions.
Article
Multidisciplinary Sciences
Shivangi Shree, Delphine Lagarde, Laurent Lombez, Cedric Robert, Andrea Balocchi, Kenji Watanabe, Takashi Taniguchi, Xavier Marie, Iann C. Gerber, Mikhail M. Glazov, Leonid E. Golub, Bernhard Urbaszek, Ioannis Paradisanos
Summary: Efficient second-harmonic generation (SHG) occurs for crystals with broken inversion symmetry, such as transition metal dichalcogenide monolayers. Here the authors show SHG tuning in bilayer MoS2 - an inversion-symmetric crystal - mediated by interlayer excitons.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Yovan de Coene, Stijn Jooken, Olivier Deschaume, Valerie Van Steenbergen, Pieter Vanden Berghe, Chris Van den Haute, Veerle Baekelandt, Geert Callewaert, Stijn Van Cleuvenbergen, Thierry Verbiest, Carmen Bartic, Koen Clays
Summary: In this study, it is found that endogenous second-harmonic generation (SHG) from live cells is highly sensitive to changes in transmembrane potential (TMP), indicating the potential of using SHG as a label-free read-out tool for electrogenic cells.
Article
Chemistry, Multidisciplinary
Lun Qu, Lu Bai, Chunyan Jin, Qiang Liu, Wei Wu, Bofeng Gao, Juntao Li, Wei Cai, Mengxin Ren, Jingjun Xu
Summary: Metasurfaces is an intriguing framework for nonlinear optics, offering compact design and flexibility in manipulating light. However, the nonlinearity is often limited due to the short interaction lengths with light. In this study, we demonstrate a record high efficiency of second harmonic generation using LN membrane metasurfaces, significantly improving the efficiency of nonlinear metasurfaces.
Article
Multidisciplinary Sciences
Gao Sheng, Hongyan Yuan, Lu Jin, Suman Ranjit, Julia Panov, Xun Lu, Moshe Levi, Robert I. Glazer
Summary: The study identified fibrosis as a crucial factor in tumor progression, and demonstrated that using the LXR agonist DMHCA could reduce fibrosis, improve immune surveillance, decrease tumor progression and enhance the efficacy of cancer therapy.
Article
Chemistry, Multidisciplinary
Young Chul Kim, Hoseong Yoo, Van Tu Nguyen, Soonil Lee, Ji-Yong Park, Yeong Hwan Ahn
Summary: In this study, a high-speed second-harmonic generation (SHG) imaging technique was used for in situ characterization of transition-metal dichalcogenide (TMD) monolayers and twisted bilayers. The SHG imaging allowed rapid identification of crystallinity, orientation, and homogeneity of the TMD with a speed of 1 frame/s. The research also revealed the SHG peak intensity and angles variation with the twist angle in twisted bilayer MoS2 under strong interlayer coupling, as well as the threshold behavior of laser-induced ablation in MoS2 monolayers and bilayers.
Article
Biochemistry & Molecular Biology
Thomas Kellerer, Janko Janusch, Christian Freymueller, Adrian Ruehm, Ronald Sroka, Thomas Hellerer
Summary: This study utilized two different fluorescence lifetime measurement techniques to investigate the factors affecting fluorescence lifetime results, showing consistent results and revealing the strongest influencing factor on fluorescence lifetime. It also provides quantitative guidance on how to choose the most suitable technique and perform experiments correctly to obtain consistent fluorescence lifetime results.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Yann Foucaud, Bertrand Siboulet, Magali Duvail, Alban Jonchere, Olivier Diat, Rodolphe Vuilleumier, Jean-Francois Dufreche
Summary: A breakthrough method has been developed to predict and interpret SHG signal at the atomic level, showing the significant impact of bulk quadrupole contribution in the SHG response. The experimental results are in unprecedented agreement with the simulated responses.
Article
Optics
Junjun Ma, Fei Xie, Weijin Chen, Jiaxin Chen, Wei Wu, Wei Liu, Yuntian Chen, Wei Cai, Mengxin Ren, Jingjun Xu
Summary: LN metasurfaces with controllable SHG properties have been experimentally demonstrated, showing distinct enhancements for the SHG efficiency at Mie resonances. By changing geometric parameters and resonances of metasurfaces, authors manage to selectively boost the SHG efficiency at different wavelengths, paving the way for developing novel compact nonlinear light sources with high flexibility for applications like biosensing and optical communications.
LASER & PHOTONICS REVIEWS
(2021)
Review
Biochemistry & Molecular Biology
Ronald M. Evans, Zong Wei
Summary: Pancreatic beta cells secrete insulin in response to glucose, and this process is regulated by multiple factors, including input signals from other organ systems. Impaired beta cell function is involved in the development of type 2 diabetes, and enhancing beta cell function through inter-organ communication, such as GLP-1 signaling, has been an effective therapeutic strategy.
Article
Multidisciplinary Sciences
Nasiha S. Ahmed, Jovylyn Gatchalian, Josephine Ho, Mannix J. Burns, Nasun Hah, Zong Wei, Michael Downes, Ronald M. Evans, Diana C. Hargreaves
Summary: This study found that the noncanonical BAF complex (ncBAF), a variant of the SWI/SNF complex, regulates the expression of secondary-response genes in the interferon response pathway. The BRD9 subunit of the ncBAF complex plays a specific role in the activation of interferon-stimulated genes (ISGs). Inhibition of BRD9 led to a reduction in ISG expression following endotoxin stimulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Chemistry, Analytical
Belen Torrado, Leonel Malacrida, Suman Ranjit
Summary: The phasor approach improves the analysis of fluorescence lifetime imaging and hyperspectral fluorescence imaging by increasing analytical speed, reducing data dimensionality, and utilizing the reciprocity principle in the phasor space. It allows for the calculation of phasor coordinates and fractional intensity contributions from multiple fluorescent species, even in the presence of complex fluorescence lifetime decays.
Article
Biochemistry & Molecular Biology
Ryan Kurtz, Andrew Libby, Bryce A. Jones, Komuraiah Myakala, Xiaoxin Wang, Yichien Lee, Grace Knoer, Julia N. Lo Cascio, Michaela McCormack, Grace Nguyen, Elijah N. D. Choos, Olga Rodriguez, Avi Z. Rosenberg, Suman Ranjit, Christopher Albanese, Moshe Levi, Carolyn M. Ecelbarger, Blythe D. Shepard
Summary: SGLT2 inhibition has emerged as a new therapeutic target for patients with diabetes and kidney disease. This study using an obese TallyHo mouse model found that modulation of renal glucose handling can decrease circulating triglycerides and lipid accumulation in the liver, improve liver structure and function, and promote healthy adipose expansion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Multidisciplinary Sciences
Yanhong Shi, Ronald M. Evans, Fred H. Gage
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Endocrinology & Metabolism
Vincent Giguere, Ronald M. Evans
Summary: This review highlights the landmark discovery of the retinoic acid receptor (RAR) and subsequent studies that have enriched our understanding of the molecular mechanisms by which a low-abundant dietary compound is essential for life.
JOURNAL OF MOLECULAR ENDOCRINOLOGY
(2022)
Article
Gastroenterology & Hepatology
Suzanne R. Sharpton, Tae Gyu Oh, Egbert Madamba, Chenjingyi Wang, Ruth T. Yu, Annette R. Atkins, Tao Huan, Michael Downes, Michael R. Evans, Rohit Loomba
Summary: This study provides evidence for a gut metagenome-derived signature with high diagnostic accuracy for predicting hepatic decompensation and mortality risk in patients with NAFLD-related cirrhosis.
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Multidisciplinary Sciences
Vera J. M. Nies, Dicky Struik, Sihao Liu, Weilin Liu, Janine K. Kruit, Michael Downes, Tim van Zutphen, Henkjan J. Verkade, Ronald M. Evans, Johan W. Jonker
Summary: This study found that FGF1 can increase glucose uptake in adipocytes by activating GLUT4 protein. Furthermore, prolonged exposure to FGF1 can stimulate adipocyte glucose uptake by transcription of GLUT1 protein.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Gastroenterology & Hepatology
Daniel Q. Huang, Michael Downes, Ronald M. Evans, Joseph L. Witztum, Christopher K. Glass, Rohit Loomba
Summary: The global burden of nonalcoholic fatty liver disease (NAFLD) is increasing, with individuals with NAFLD at higher risk for cardiovascular disease, sharing multiple disease mechanisms.
SEMINARS IN LIVER DISEASE
(2022)
Article
Multidisciplinary Sciences
Nadia Sarfraz, Emilia Moscoso, Therese Oertel, Harrison J. Lee, Suman Ranjit, Esther Braselmann
Summary: Visualizing RNA in live cells is crucial for understanding its dynamics and functions in complex cellular environments. Current efforts involve genetically fusing RNA aptamer tags to the RNA of interest and using fluorescent molecules for detection. The Riboglow-FLIM platform presented in this study offers a smaller tag and superior cell contrast compared to intensity-based detection, allowing for simultaneous visualization of multiple RNAs using different lifetime-based tags.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yohei Abe, Eric R. Kofman, Maria Almeida, Zhengyu Ouyang, Filipa Ponte, Jasmine R. Mueller, Grisel Cruz-Becerra, Mashito Sakai, Thomas A. Prohaska, Nathanael J. Spann, Ana Resende-Coelho, Jason S. Seidman, Joshua D. Stender, Havilah Taylor, Weiwei Fan, Verena M. Link, Isidoro Cobo, Johannes C. M. Schlachetzki, Takao Hamakubo, Kristen Jepsen, Juro Sakai, Michael Downes, Ronald M. Evans, Gene W. Yeo, James T. Kadonaga, Stavros C. Manolagas, Michael G. Rosenfeld, Christopher K. Glass
Summary: This study found that RANK signaling can convert the NCoR/HDAC3 co-repressor complex into a co-activator of AP-1 and NF-kB target genes, promoting mouse osteoclast differentiation. Mechanistically, RANK signaling activates the transcriptional co-activator PGC1(3 and inhibits HDAC3 activity for acetylated histone H3. Non-coding RNAs Dancr and Rnu12 play important roles in RANKL-induced osteoclast differentiation.
Article
Multidisciplinary Sciences
Min Young Kim, Hyun Young Shin, Sung Chun Cho, Sohae Yang, Aseer Intisar, Hyeong Jung Woo, Youn-Suk Choi, Chang-Lim You, Jong-Sun Kang, Yun-Il Lee, Sang Chul Park, Kyungmoo Yea, Tae Gyu Oh, Michael Downes, Ronald M. Evans, Minseok S. Kim
Summary: This study demonstrates the potential of a silver electroceutical technology to treat sarcopenia. Through the use of a high-throughput electrical stimulation screening platform, specific electrical stimulation conditions were identified that improved muscle tissue characteristics in aged mice and human-derived aged muscle cells. This research provides insight into personalized bioelectronic medicine.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Corina E. Antal, Tae Gyu Oh, Stefan Aigner, En-Ching Luo, Brian A. Yee, Tania Campos, Herve Tiriac, Katherine L. Rothamel, Zhang Cheng, Henry Jiao, Allen Wang, Nasun Hah, Elizabeth Lenkiewicz, Jan C. Lumibao, Morgan L. Truitt, Gabriela Estepa, Ester Banayo, Senada Bashi, Edgar Esparza, Ruben M. Munoz, Jolene K. Diedrich, Nicole M. Sodir, Jasmine R. Mueller, Cory R. Fraser, Erkut Borazanci, David Propper, Daniel D. Von Hoff, Christopher Liddle, Ruth T. Yu, Annette R. Atkins, Haiyong Han, Andrew M. Lowy, Michael T. Barrett, Dannielle D. Engle, Gerard I. Evan, Gene W. Yeo, Michael Downes, Ronald M. Evans
Summary: This study identifies a druggable RNA-binding protein cascade mediated by super-enhancers that supports PDAC growth through enhanced mRNA translation. The cascade is driven by a super-enhancer associated with the RBP heterogeneous nuclear ribonucleoprotein F, which stabilizes protein arginine methyltransferase 1 to control the translational mediator ubiquitin-associated protein 2-like. These genes and the regulatory super-enhancer are essential for PDAC growth and are coordinately regulated by Myc oncogene.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Ting Fu, Tao Huan, Gibraan Rahman, Hui Zhi, Zhenjiang Xu, Tae Gyu Oh, Jian Guo, Sally Coulter, Anupriya Tripathi, Cameron Martino, Justin L. Mccarville, Qiyun Zhu, Fritz Cayabyab, Brian Low, Mingxiao He, Shipei Xing, Fernando Vargas, Ruth T. Yu, Annette Atkins, Christopher Liddle, Janelle Ayres, Manuela Raffatellu, Pieter C. Dorrestein, Michael Downes, Rob Knight, Ronald M. Evans
Summary: Colorectal cancer is driven by genomic alterations and dietary influences, and the gut microbiome and metabolome play important roles in disease progression. A multi-omics study on mice reveals that diet is the major driver of microbiome and metabolome differences, with high-fat diet causing decreased diversity and changes in cecal metabolites.
Article
Biophysics
Nadia Sarfraz, Harrison J. Lee, Morgan K. Rice, Emilia Moscoso, Luke K. Shafik, Eric Glasgow, Suman Ranjit, Ben J. Lambeck, Esther Braselmann
Summary: The study establishes a RNA tagging and imaging platform called Riboglow-FLIM for complex cellular imaging applications. By evaluating the FLIM capabilities, they were able to optimize the entire process and successfully visualize and detect the cell-to-cell heterogeneity of noncoding RNA in live zebrafish embryos.
BIOPHYSICAL REPORTS
(2023)
