Article
Chemistry, Analytical
Akram Hajinia, Tahereh Heidari
Summary: The study developed a simple, low-cost and sensitive micro fluidic paper-based analytical device for analysis of gold in geological samples. Through optimization, efficient detection of gold with good accuracy was achieved.
MICROCHEMICAL JOURNAL
(2021)
Article
Biophysics
Zhenzhen Han, Xinyan Peng, Yi Yang, Jia Yi, Dan Zhao, Qiyuan Bao, Shuping Long, Sai-Xi Yu, Xin-Xin Xu, Baohong Liu, Yan-Jun Liu, Yuhui Shen, Liang Qiao
Summary: In this study, a method combining microfluidics and surface-enhanced Raman spectroscopy (SERS) was developed to isolate exosomes from plasma and profile exosomal biomarkers for osteosarcoma diagnosis. The method showed efficient isolation of exosomes directly from human plasma and could profile exosomes based on protein biomarkers with a low detection limit. The analysis of plasma-derived exosomes from osteosarcoma patients and healthy controls revealed significantly higher levels of specific protein biomarkers on exosomes from osteosarcoma patients. A classification model was built based on the exosomal biomarkers, achieving high sensitivity, specificity, and accuracy. This method, which is simple and cost-effective, has great potential for clinical cancer diagnosis as a liquid biopsy technique.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Lang Li, Yang Lu, Ziting Qian, Zhaoyan Yang, Kuo Yang, Shenfei Zong, Zhuyuan Wang, Yiping Cui
Summary: A microfluidic surface enhanced Raman spectroscopy (SERS) biosensor was developed for ultrasensitive and reliable detection of dopamine (DA) in serum, using DNA-assisted synthesis of ortho-nanodimers (DaSON) strategy. The SERS sensor demonstrated ultrahigh sensitivity and high accuracy, with a limit of detection as low as 10 aM in phosphate buffer saline and 10 fM in serum. Quantitative monitoring of extracellular DA released from living neurons and quantification of DA in human blood samples were successfully achieved, providing a promising tool for in-vitro investigation of neurological processes and clinical examination of dopaminergic disorders.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Uzeyir Dogan, Ferah Sucularli, Ender Yildirim, Demet Cetin, Zekiye Suludere, Ismail Hakki Boyaci, Ugur Tamer
Summary: Pathogen detection is still a challenging issue in public health, especially in food products. Researchers have developed a method using a capillary-driven microfluidic chip and surface-enhanced Raman scattering measurements for rapid detection of Escherichia coli. This method can detect very low concentrations of E. coli within 60 minutes.
Article
Chemistry, Analytical
Yang Lu, Changbiao Zhan, Liandong Yu, Yiyue Yu, Huakun Jia, Xiaozhe Chen, Dongzhi Zhang, Rongke Gao
Summary: We developed a novel multifunctional nanocone array as a solid immunoassay plate and SERS substrate for the simultaneous detection of PSA and thrombin based on a microfluidic chip. Compared to traditional immunoassays, this nanocone array with a deposited gold film provides a larger surface area for efficient target antigen capture. By integrating this microfluidic chip, we achieved simultaneous detection of PSA and thrombin with high sensitivity. This detection strategy shows great potential for early prostate cancer diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Nanoscience & Nanotechnology
Xiaojia Liu, Shikun Yang, Yang Li, Bo Wang, Jinhong Guo, Xing Ma
Summary: Metallic nanodot-encapsulated hollow mesoporous silica nanoparticles with adjustable inner metallic components were successfully prepared by coating sacrificial polystyrene nanoparticles with various metals and wrapping them with mesoporous silica. These nanoparticles were demonstrated to be effective as smart SERS probes for distinguishing between large molecules and small analytes, as well as for detecting H2O2, cancer cells, glucose, and uric acid with high sensitivity and specificity. Furthermore, this versatile fabrication strategy holds great promise for developing a wide range of M-HMSNPs for diverse applications including catalysis, energy storage, and theranostics.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Nanoscience & Nanotechnology
Rongke Gao, Yuanshuo Mao, Chao Ma, Yeru Wang, Huakun Jia, Xiaozhe Chen, Yang Lu, Dongzhi Zhang, Liandong Yu
Summary: We developed a new plasmonic nanostripe microcone array (PNMA) substrate-integrated microfluidic chip for simultaneous surface enhanced Raman scattering (SERS)-based immunoassay of cardiac markers CK-MB and cTnI. The chip features a gold film coated microcone array with nanostripes that provides a large surface area for antibody conjugation and acts as a SERS-active substrate. This unique nano-microhierarchical structure enhances the sensitivity and applicability of SERS-based immunoassay, allowing for simultaneous detection with a low detection limit.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Junjie Chen, Suyang Li, Fuqi Yao, Wanbing Xu, Yunfeng Li, Qiang Chen, Pei Liang
Summary: In this study, a high-throughput method for the preparation of monodisperse submicron silver particles using S-shaped microfluidic chips was demonstrated. The particle size and morphology of the silver particles could be controlled by adjusting the concentration of the reducing agent ascorbic acid and the stabilizer PVP. The synthesized submicron silver particles showed ultra-sensitive SERS detection, with a detection limit of 10(-9) M for rhodamine 6G (R6G).
Article
Engineering, Electrical & Electronic
Tania K. Naqvi, Abhilash Bajpai, Sarthak Dwivedi, Manish Bhaiyya, Sanket Goel, Prabhat K. Dwivedi
Summary: In this study, a low-cost, label-free flexible paper-based microfluidic SERS sensor was developed for thiram detection. Hydrophilic wells of different sizes were created on paper using wax-coated mould printing, and the hydrophilic nature of the wells was confirmed. Silver nanoparticles decorated graphene oxide nano-hybrid (Ag-rGO) was synthesized and drop-casted onto the wells for thiram detection, achieving fM sensitivity with a low limit of detection and high enhancement factor. The reproducibility and uniformity of the fabricated substrates were tested, showing good results. The Ag-rGO/wax paper (Ag-rGO/WP) SERS sensor has the potential to be used as a portable sensing platform for food safety applications.
SENSORS AND ACTUATORS A-PHYSICAL
(2023)
Article
Spectroscopy
Yuntong Huang, Weizhong Chen, Mingyou Dong, Nana Li, Lianghui Chen, Li Ling, Qijiang Xu, Min Lin, Zhiyong Xing
Summary: A facile fluorescence probe BQBH was synthesized and investigated on its spectrum property. The BQBH showed high sensitivity and selectivity for Cd2+ with the lowest detection of 0.14 μM. The 1:1 binding ratio between BQBH and Cd2+ was confirmed by various analyses, and its applications in test paper, smart phone, and cell image were also studied.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Yuxiang Ji, Gaozhe Cai, Cheng Liang, Zehang Gao, Weimin Lin, Zizhen Ming, Shilun Feng, Hongwei Zhao
Summary: To realize rapid, simple, and accurate detection of Okadaic acid (OA) in shellfish, a novel microfluidic immunosensor based on magnetic beads modified with a highly specific and sensitive monoclonal antibody (mAb) against OA was developed and used in conjunction with smartphone imaging. The method achieves on-site detection results within 1 h with an IC50 value of 3.30 ng/mL for OA and a limit of detection (LOD) of 0.49 ng/mL. Real sample analysis showed good accuracy and precision, and the results were reported in a specially designed smartphone app. The microfluidic immunosensor provides a reliable technical solution for detecting OA residues in shellfish, with the advantages of simple operation, rapid detection, and high sensitivity.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Rongke Gao, Feng Chen, Dongdong Yang, Lihua Zheng, Tongmei Jing, Huakun Jia, Xiaozhe Chen, Yang Lu, Shenghao Xu, Dongzhi Zhang, Liandong Yu
Summary: We developed a new pump-free hybrid microfluidic chip for simultaneous detection of creatine kinase MB isoenzyme and cardiac troponin cardiac markers. The integration of paper and PDMS microfluidic chip offers portability, low-cost, and high sensitivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Optics
Shi Bai, Xueli Ren, Kotaro Obata, Yoshihiro Ito, Koji Sugioka
Summary: This paper introduces the application of liquid interface assisted SERS (LI-SERS) method, which has high sensitivity and versatility for label-free trace detection of bio-molecules. The ultrahigh sensitivity of the LI-SERS method is demonstrated through DNA discrimination and quantitative detection of beta-Amyloid. These results suggest the potential application of LI-SERS method in early-stage diagnosis of diseases such as virus infection and Alzheimer's disease.
OPTO-ELECTRONIC ADVANCES
(2022)
Article
Optics
Yong Wei, Chen Shi, Yonghui Zhang, Chunbiao Liu, Yixiong Tang, Puxi Ren, Chen Wang, Yu Zhang, Zhihai Liu
Summary: In this paper, a temperature-compensated optical fiber SPR microfluidic sensor based on micro-nano 3D printing is proposed. The sensor utilizes optical fiber micro-machining technology to design dual sensing areas for simultaneous collection and temperature compensation. It demonstrates excellent specificity and sensitivity for the detection of berberine biomolecules.
Article
Materials Science, Multidisciplinary
Chansi, Satyapal Chaudhary, Ashish Mani, Lalit M. Bharadwaj, Tinku Basu
Summary: The hand-held electrochemical sensor interfaced with a smartphone allows rapid monitoring of organophosphate in vegetable extract on-site. Its simple and inexpensive architecture, combined with a miniaturized potentiostat powered by rechargeable battery, inspired the construction of adaptable sensors through software modulation, thus opening new avenues for affordable sensor and wearable device fabrication.
Article
Biochemical Research Methods
Bin Yang, Xingye Chen, Yina Wang, Siyu Feng, Veronica Pessino, Nico Stuurman, Nathan H. Cho, Karen W. Cheng, Samuel J. Lord, Linfeng Xu, Dan Xie, R. Dyche Mullins, Manuel D. Leonetti, Bo Huang
Article
Chemistry, Multidisciplinary
Upendra Chitgupi, Nikhila Nyayapathi, Jeesu Kim, Depeng Wang, Boyang Sun, Changning Li, Kevin Carter, Wei-Chico Huang, Chulhong Kim, Jun Xia, Jonathan F. Lovell
ADVANCED MATERIALS
(2019)
Review
Biochemical Research Methods
Nikhila Nyayapathi, Jun Xia
JOURNAL OF BIOMEDICAL OPTICS
(2019)
Review
Biochemical Research Methods
Linfeng Xu, Anyang Wang, Xiangpeng Li, Kwang W. Oh
Article
Engineering, Biomedical
Nikhila Nyayapathi, Rachel Lim, Huijuan Zhang, Wenhan Zheng, Yuehang Wang, Melinda Tiao, Kwang W. Oh, X. Cynthia Fan, Ermelinda Bonaccio, Kazuaki Takabe, Jun Xia
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2020)
Article
Biochemical Research Methods
Nikhila Nyayapathi, Huijuan Zhang, Emily Zheng, Srinidhi Nagarajan, Ermelinda Bonaccio, Kazuaki Takabe, Xinhao C. Fan, Jun Xia
Summary: The study developed a photoacoustics-based imaging system that can differentiate between malignant tumors and healthy breast tissues, showing potential for improving breast cancer screening.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Biochemical Research Methods
Jorge Tordera Mora, Xiaohua Feng, Nikhila Nyayapathi, Jun Xia, Liang Gao
Summary: The study aims to develop a beamforming algorithm - generalized spatial coherence (GSC) - that unifies and outperforms previous beamformers, improving the resolution and contrast of photoacoustic image reconstruction. The results show that GSC surpasses the imaging metrics of previous coherence-based beamformers in both simulation and experiments, making it an innovative reconstruction algorithm expanding the applications of PACT.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Chemistry, Multidisciplinary
Linfeng Xu, Xiangpeng Li, Wenzong Li, Kai-chun Chang, Hyunjun Yang, Nannan Tao, Pengfei Zhang, Emory M. Payne, Cyrus Modavi, Jacqueline Humphries, Chia-Wei Lu, Adam R. Abate
Summary: This paper describes a simple method to fabricate substrates patterned with micrometer-scale wells, which can enhance the sensitivity of laser desorption ionization mass spectrometry and concentrate cells and beads for cell-based assays.
ADVANCED MATERIALS
(2022)
Article
Multidisciplinary Sciences
Linfeng Xu, Kai-Chun Chang, Emory M. Payne, Cyrus Modavi, Leqian Liu, Claire M. Palmer, Nannan Tao, Hal S. Alper, Robert T. Kennedy, Dale S. Cornett, Adam R. Abate
Summary: Enzymes have diverse activities that surpass chemical catalysts, but engineering them for novel or enhanced activity is hampered by limited technologies for sensing product formation. This study presents a general and scalable approach that uses host cell metabolism as a biosensor to infer product formation, enabling the discovery of unexpected products and mapping the effects of mutagenesis through mass spectrometry. Additionally, a screening method combining microfluidics and mass spectrometry is reported for mapping catalysis, characterizing product range, and recovering sequences of variants with desired activities.
NATURE COMMUNICATIONS
(2021)
Article
Computer Science, Interdisciplinary Applications
Huijuan Zhang, Wei Bo, Depeng Wang, Anthony DiSpirito, Chuqin Huang, Nikhila Nyayapathi, Emily Zheng, Tri Vu, Yiyang Gong, Junjie Yao, Wenyao Xu, Jun Xia
Summary: In this study, a novel method Deep-E is proposed to improve the elevational resolution of a linear array by converting the three-dimensional problem into a two-dimensional problem. It shows high computational efficiency and can enhance image resolution significantly. Experimental results demonstrate that Deep-E can improve elevational resolution at least four times and recover the true size of objects.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2022)
Article
Multidisciplinary Sciences
Justin L. Madrigal, Nathan G. Schoepp, Linfeng Xu, Codian S. Powell, Cyrille L. Delley, Christian A. Siltanen, Jay Danao, Maithreyan Srinivasan, Russell H. Cole, Adam R. Abate
Summary: Cell-cell interactions play a crucial role in biological systems, but current methods for studying these interactions are limited in scalability. This study presents a novel approach for cell interaction profiling using high-throughput droplet microfluidics, allowing for accurate grouping and characterization of cell interactions on a large scale.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemical Research Methods
Mucong Li, Nikhila Nyayapathi, Hailey I. Kilian, Jun Xia, Jonathan F. Lovell, Junjie Yao
Proceedings Paper
Optics
Nikhila Nyayapathi, Huijuan Zhang, Melinda Tiao, X. Cynthia Fan, Ermelinda Bonaccio, Kazuaki Takabe, Jun Xia
PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2020
(2020)
Article
Materials Science, Biomaterials
Hailey Kilian, Homan Kang, Nikhila Nyayapathi, Takeshi Fukuda, Eeswar Adluru, Huijuan Zhang, Breandan Quinn, Jun Xia, Hak Soo Choi, Jonathan F. Lovell
BIOMATERIALS SCIENCE
(2020)
Article
Engineering, Biomedical
Huijuan Zhang, Hongyu Li, Nikhila Nyayapathi, Depeng Wang, Alisa Le, Leslie Ying, Jun Xia
COMPUTERIZED MEDICAL IMAGING AND GRAPHICS
(2020)
