Article
Pharmacology & Pharmacy
Yanxuan Xiao, Hangfan Zhou, Lu Jiang, Rui Liu, Qianming Chen
Summary: Taste is mediated by specialized neuroepithelial cells called taste receptor cells located at tongue papillae. Ion channels play a crucial role in various cell physiological activities, while epigenetic modifications regulate gene expression without altering DNA sequence.
PHARMACOLOGICAL RESEARCH
(2021)
Review
Ecology
Daniel Berning, Joshua B. Gross
Summary: Animals in subterranean environments evolve regressive and constructive features. While regressive traits like vision and pigmentation are reduced, constructive traits like non-visual sensation are commonly enhanced. The gustatory system, an important constructive sensory feature in cave animals, remains poorly understood. This review highlights the differences in gustation between cave and surface fish, identifies knowledge gaps in constructive taste system evolution, and emphasizes the need for further research on the genetic and developmental mechanisms underlying constructive traits.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2023)
Review
Physiology
Kenneth W. Dunn, Bruce A. Molitoris, Pierre C. Dagher
Summary: The Indiana O'Brien Center is a research center funded by the NIH, dedicated to developing and disseminating advanced optical microscopy methods to support renal researchers worldwide. The center provides advanced imaging capabilities through collaborations and a unique fellowship program to renal researchers globally.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Shuge Liu, Ping Zhu, Yulan Tian, Yating Chen, Yage Liu, Wei Chen, Liping Du, Chunsheng Wu
Summary: This review provides a comprehensive introduction to the preparation and application of taste bud organoids for studying chemical sensing mechanisms. It discusses their central roles in disease modeling, biological sensing, gene regulation, and signal transduction, and explores the current challenges and future development trends in the field of taste bud organoid research.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Neurosciences
Tao Huang, Lisa C. Ohman, Anna V. Clements, Zachary D. Whiddon, Robin F. Krimm
Summary: Taste neurons exhibit a high level of branching diversity, suggesting differential convergence may exist between neurons. Additionally, the morphology of taste neurons may vary with the type of taste quality they perceive.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Hayeon Sung, Iva Vesela, Hannah Driks, Carrie R. Ferrario, Charlotte M. Mistretta, Robert M. Bradley, Monica Dus
Summary: Consuming sucrose has selective and modality-specific effects on peripheral taste nerve responses and taste bud cells, but does not affect the number and size of taste buds. Replacing sucrose with water can fully restore these effects.
Review
Food Science & Technology
Fei Xie, Jiakun Shen, Tianyi Liu, Min Zhou, Lee J. Johnston, Jingwen Zhao, Hongfu Zhang, Xi Ma
Summary: Nutrient sensing is crucial for maintaining body homeostasis and physiological functions. Gut taste receptors play important roles in sensing nutrients and regulating intestinal physiological activities, as well as appetite, body weight, and gut microbiota. Dysfunction of gut taste receptors may lead to digestive and metabolic diseases.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Chemistry, Multidisciplinary
Shuang Huang, Tao Zhang, Hongbo Li, Mingyue Zhang, Xingxing Liu, Dongxin Xu, Hao Wang, Zhiran Shen, Qianni Wu, Jun Tao, Wenhao Xia, Xi Xie, Fanmao Liu
Summary: Electric stimulation on the tongue can evoke taste sensations, and a portable flexible tongue electrode array system has been developed to provide electrical stimulation and signal mapping. Tests on rats' tongues show that appropriate electrical stimulation can induce multiple sensations simultaneously, with no significant distinction in the distribution of each sensation on the tongue.
Article
Neurosciences
Nicholas W. Villarino, Yasmeen M. F. Hamed, Britya Ghosh, Adrienne E. Dubin, Amanda H. Lewis, Max A. Odem, Meaghan C. Loud, Yu Wang, M. Rocio Servin-Vences, Ardem Patapoutian, Kara L. Marshall
Summary: Sensory neurons detect forces from the environment and internal organs to regulate physiology. PIEZO2 is a critical mechanosensory ion channel involved in touch, proprioception, and bladder stretch sensation. The use of the fluorescent dye FM 1-43 has revealed the functional role of PIEZO2-expressing neurons in detecting forces and has facilitated the study of mechanosensory processes in various organ systems.
Article
Neurosciences
Anindya Ganguly, Manali Dey, Christi Scott, Vi-Khoi Duong, Anupama Arun Dahanukar
Summary: Food choice in animals can change with internal nutritional state and dietary conditions. In fruit flies, macronutrient imbalances in diet can lead to compensatory behavioral shifts, as well as changes in taste sensitivity. This study found that the preferences for specific macronutrients were modified in response to diet composition, with differences in onset of changes in taste sensitivity and behavior. Additionally, the molecular mechanisms behind these changes were investigated, showing the involvement of certain genes in modulating taste preferences.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Engineering, Multidisciplinary
Wenbo Zheng, Yan Shi, Xiuxin Xia, Yuxiang Ying, Hong Men
Summary: This paper proposes a method for evaluating the taste sensation of an e-tongue based on human taste conduction mechanisms, and uses a parameter optimization algorithm to improve the evaluation performance. The results show that the method has improved dynamic characteristics and achieves good performance in pattern recognition and taste evaluation.
MEASUREMENT SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yuta Yoshida, Misa Miyazaki, Yuhei Yajima, Atsushi Toyoda
Summary: This study utilized a mouse model to investigate the effects of psychosocial stress on peripheral taste-sensing systems, revealing that psychosocial stress can downregulate the mRNA levels of sweet and umami taste receptor subunits.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yuichi Masuda, Shogo Matsuda, Takuya Kotani, Daisuke Nishioka, Shin Ota, Takafumi Hosokawa, Shimon Ishida, Tohru Takeuchi
Summary: This study investigates the pathomechanism of peripheral neuropathy in microscopic polyangiitis (MPA) and identifies potential biomarkers for diagnosis and severity assessment. The study shows that serum levels of tissue inhibitor of metalloproteinase-1 (TIMP-1) is associated with motor neuropathy in MPA and could be a useful biomarker for diagnosing and evaluating the severity of motor neuropathy in MPA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yuanyuan Wang, Junjia Luan, Xuhua Tang, Wenhui Zhu, Yongxia Xu, Ying Bu, Jianrong Li, Fangchao Cui, Xuepeng Li
Summary: Umami peptides have attracted attention in the field of food umami science and are considered the key for umami agent development. In this study, virtual screening, molecular docking, and electronic tongue analysis were used to identify umami peptides from Atlantic cod myosin. Twenty-three putative umami peptides were screened and found to bind to the T1R3 cavity, exhibiting hydrogen bonding and electrostatic interactions. Twelve synthetic peptides tested on the electronic tongue showed umami taste and synergistic effects with monosodium glutamate. This study provides a method for rapid screening of umami peptides from marine protein resources and lays the foundation for their application in the food industry.
Article
Engineering, Electrical & Electronic
Hengyang Wang, Xianghao Zhan, Li Liu, Asif Ullah, Huiyan Li, Han Gao, You Wang, Ruifen Hu, Guang Li
Summary: This study investigates the improvement of generalizability and transferability of taste sensation models developed with sEMG data by innovatively applying two methods: domain regularized component analysis (DRCA) and conformal prediction with shrunken centroids (CPSC). The effectiveness of these methods is explored in an unlabeled data augmentation process on six subjects. The results show that DRCA significantly improves classification accuracy, while CPSC does not guarantee accuracy improvement. The combination of DRCA and CPSC presents a statistically significant improvement in classification accuracy on six subjects.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2022)
Article
Biochemical Research Methods
Zhiduo Zhang, Yujie Zheng, Tienan Xu, Avinash Upadhya, Yean Jin Lim, Alexander Mathews, Lexing Xie, Woei Ming Lee
BIOMEDICAL OPTICS EXPRESS
(2020)
Article
Biophysics
Yujie Zheng, Samantha J. Montague, Yean J. Lim, Tao Xu, Tienan Xu, Elizabeth E. Gardiner, Woei Ming Lee
Summary: This study introduces a label-free multimodal quantitative imaging flow assay that can evaluate the process of blood clot formation in real time in vitro. The assay combines rotating optical coherent scattering microscopy and quantitative phase microscopy to accurately record changes in intrathrombus mass and platelet membrane shape, providing a new research tool for studying the mechanism of thrombus formation.
BIOPHYSICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Michelle M. McDonald, Weng Hua Khoo, Pei Ying Ng, Ya Xiao, Jad Zamerli, Peter Thatcher, Wunna Kyaw, Karrnan Pathmanandavel, Abigail K. Grootveld, Imogen Moran, Danyal Butt, Akira Nguyen, Alexander Corr, Sean Warren, Mate Biro, Natalie C. Butterfield, Siobhan E. Guilfoyle, Davide Komla-Ebri, Michael R. G. Dack, Hannah F. Dewhurst, John G. Logan, Yongxiao Li, Sindhu T. Mohanty, Niall Byrne, Rachael L. Terry, Marija K. Simic, Ryan Chai, Julian M. W. Quinn, Scott E. Youlten, Jessica A. Pettitt, David Abi-Hanna, Rohit Jain, Wolfgang Weninger, Mischa Lundberg, Shuting Sun, Frank H. Ebetino, Paul Timpson, Woei Ming Lee, Paul A. Baldock, Michael J. Rogers, Robert Brink, Graham R. Williams, J. H. Duncan Bassett, John P. Kemp, Nathan J. Pavlos, Peter Croucher, Tri Giang Phan
Summary: Research has shown that RANKL-stimulated osteoclasts can undergo fission to form osteomorphs, which are involved in the regulation of bone resorption and could be targeted for the treatment of skeletal diseases. Single-cell RNA sequencing revealed that osteomorphs are transcriptionally distinct from osteoclasts and macrophages, expressing non-canonical osteoclast genes associated with bone phenotypes when deleted in mice.
Correction
Biochemistry & Molecular Biology
Michelle M. McDonald, Weng Hua Khoo, Pei Ying Ng, Ya Xiao, Jad Zamerli, Peter Thatcher, Wunna Kyaw, Karrnan Pathmanandavel, Abigail K. Grootveld, Imogen Moran, Danyal Butt, Akira Nguyen, Alexander Corr, Sean Warren, Mate Biro, Natalie C. Butterfield, Siobhan E. Guilfoyle, Davide Komla-Ebri, Michael R. G. Dack, Hannah F. Dewhurst, John G. Logan, Yongxiao Li, Sindhu T. Mohanty, Niall Byrne, Rachael L. Terry, Marija K. Simic, Ryan Chai, Julian M. W. Quinn, Scott E. Youlten, Jessica A. Pettitt, David Abi-Hanna, Rohit Jain, Wolfgang Weninger, Mischa Lundberg, Shuting Sun, Frank H. Ebetino, Paul Timpson, Woei Ming Lee, Paul A. Baldock, Michael J. Rogers, Robert Brink, Graham R. Williams, J. H. Duncan Bassett, John P. Kemp, Nathan J. Pavlos, Peter I. Croucher, Tri Giang Phan
Article
Biochemical Research Methods
Jisoo Han, Seonghoon Kim, Pyonggang Choi, Sungho Lee, Yongjae Jo, Eunsoo Kim, Myunghwan Choi
Summary: The study introduces an axially elongated Bessel beam in two-photon microscopy to address the focal shift issue during imaging of taste cells, enabling accurate imaging of physiological taste stimuli.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Multidisciplinary Sciences
Yonghyeon Jo, Ye-Ryoung Lee, Jin Hee Hong, Dong-Young Kim, Junhwan Kwon, Myunghwan Choi, Moonseok Kim, Wonshik Choi
Summary: DReAM is a label-free technique for deep-tissue imaging that selectively attenuates multiple scattering, providing high-contrast images of neural fibers.
Article
Optics
Tienan Xu, Hanqi Lin, Yean J. Lim, Philip R. Nicovich, Katharina Gaus, Woei Ming Lee
Summary: Single-objective scanning light sheet (SOLS) imaging has made significant advances in volumetric bioimaging by providing low phototoxicity and high-resolution imaging. This paper proposes a technique called computational SOLS (cSOLS) that achieves light sheet imaging without the need for a remote imaging unit. cSOLS utilizes a single microlens array after the tube lens to enable lightfield imaging and compatibility with conventional epifluorescence detection. The core of cSOLS is a Fast Optical Ray (FOR) model that generates a 3D imaging volume using 2D lightfield images captured under SOLS illumination. FOR improves optical sectioning and achieves a spatial resolution of 1.59 x 1.92 x 1.39 μm³.
Article
Chemistry, Analytical
Eunchan Kim, Seonghoon Kim, Myunghwan Choi, Taewon Seo, Sungwook Yang
Summary: We introduce a new deep learning framework, HAR-CNN, for removing honeycomb artifacts in fiber bundle imaging caused by optical path blocking. HAR-CNN provides an end-to-end mapping from raw fiber bundle images to artifact-free images using a convolutional neural network (CNN). By synthesizing honeycomb patterns on regular images, HAR-CNN can learn and validate the network without requiring a large collection of ground truth data. Compared to conventional methods, HAR-CNN significantly improves honeycomb pattern removal and preserves details in the 1961 USAF chart sample. It is also GPU-accelerated for real-time processing and enhanced image mosaicking performance.
Article
Nanoscience & Nanotechnology
Yujie Zheng, Yean Jin Lim, Hanqi Lin, Tienan Xu, Carmen Longbottom, Viviane Delghingaro-Augusto, Yee Lin Thong, Christopher R. Parish, Elizabeth E. Gardiner, Woei Ming Lee
Summary: This paper introduces a method to study cell migration using live cell imaging tools and proposes a combined system that integrates label-free nanoscale and fluorescence imaging. By empirically determining optimal scanning angles, quantitative 3D spatial temporal details of cellular components can be obtained. Using this combined imaging tool, researchers analyzed the migration of platelets and endothelial cells and made key findings, such as the importance of bovine serum albumin in platelet migration and the dynamics of mitochondria during cell migration.
Article
Neurosciences
Seonghoon Kim, Hyun Seok Moon, Thanh Tan Vo, Chang-Ho Kim, Geun Ho Im, Sungho Lee, Myunghwan Choi, Seong-Gi Kim
Summary: Functional magnetic resonance imaging (fMRI) combined with optogenetic neural manipulation is a powerful tool for mapping brain-wide effective functional networks. By incorporating programmable optogenetic stimuli generated by a digital micromirror device into an MRI scanner, researchers can flexibly manipulate neural excitation in the mouse cortex. This approach allows for spatial and temporal versatility in planning photostimulation patterns, along with optical imaging and specific genetic targeting. The use of fMRI with optogenetics enables high-throughput brain-wide effective connectivity mapping, allowing for investigation of dynamic changes in functional brain states in the same animal.
Article
Chemistry, Multidisciplinary
Ju Seung Lee, Jiwoong Choi, Gha Yeon Park, Seung Ji Kang, Jae-hun Yang, Youngkyu Lee, Myunghwan Choi, Kwangmeyung Kim, Tae-il Kim
Summary: The development of fabrication technologies and new materials has significantly improved the performance of electronic devices while reducing their overall size. However, micro/nano-size electronic devices have faced issues related to isolated heat generation in concentrated areas, especially in bio-integrated devices. To address this problem, a microscale light emitting diode (mu LED)-based neural probe with an injectable heat dissipation guide made of boron nitride (BN) nanomaterials is introduced. This heat dissipation guide effectively dissipates heat, leading to improved optical output performance of mu LEDs and enhanced lighting transmission through brain tissue without thermal damage. Additionally, it shows remarkable improvement in the therapeutic effect of photodynamic therapy in mouse cancer cells.
Review
Biochemistry & Molecular Biology
Gha Yeon Park, Hyeyeong Hwang, Myunghwan Choi
Summary: This article introduces the recent advances in optical tools used to study taste transduction pathways. Optical tools that observe cellular-level functions in this field play a crucial role in understanding how taste information is processed.
MOLECULES AND CELLS
(2022)
Proceedings Paper
Engineering, Electrical & Electronic
Yujie Zheng, Samantha J. Montague, Yean Jin Lim, Tienan Xu, Elizabeth E. Gardiner, Woei Ming Lee
2020 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO)
(2020)
Proceedings Paper
Engineering, Electrical & Electronic
Yongxiao Li, Yean J. Lim, Qiongkai Xu, Lynette Beattie, Elizabeth E. Gardiner, Katharina Gaus, William R. Heath, Woei Ming Lee
2020 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO)
(2020)
Article
Biochemical Research Methods
Tienan Xu, Yean Jin Lim, Yujie Zheng, MoonSun Jung, Katharina Gaus, Elizabeth E. Gardiner, Woei Ming Lee
