Article
Chemistry, Analytical
Omair Adil, Seth B. Eddington, Keith T. Gagnon, Mohtashim H. Shamsi
Summary: Overgrowth of short tandem repeat sequences in genes can cause neurodegenerative disorders. These repeat sequences are ideal targets for label-free electrochemical detection, but their secondary structures and sporadic repeats can complicate detection. By analyzing different nucleic acid microprobe backbones, the study found that the lock-nucleic acid (LNA) microprobe can specifically detect tandem repeats and perform better with the presence of Mg2+. The LNA-based platform shows potential for the development of a reliable biosensor for genetic neurodegenerative disorders.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yao Chen, Yu Zhong, Ji-Xing Ye, Yun Lei, Ai-Lin Liu
Summary: In this study, a sensitive and selective electrochemical DNA biosensor was developed for the determination of a osteosarcoma-related survivin gene. The proposed strategy used a simple and low-cost working electrode, with no need for other substances for electrode or DNA functionalization. The mode of interaction between celestine blue (CB) and DNA was studied, and it was found that the possible mode was intercalation with a binding number of 2 and a binding constant beta of 10(12.87). The label-free electrochemical DNA biosensor showed a good linear relationship towards the target gene and had a low detection limit of 0.046 nM.
Article
Chemistry, Analytical
Meixing Li, Juan Cheng, Haitao Zheng, Jiawei Shi, Qingming Shen
Summary: A new electrochemical sensing strategy was developed for the precise detection of ultra-low abundance microRNA-21. The sensor utilized modified magnetic beads and hybridization chain reaction (HCR) to achieve signal amplification while avoiding modification fluctuation. The biosensor exhibited excellent specificity and reproducibility in complex sample analysis.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Shan-Shan Shi, Li-Ping Jia, Wei Zhang, Rong-Na Ma, Lei Shang, Xiao-Jian Li, Huai-Qing Zhao, Huai-Sheng Wang
Summary: A label-free electrochemical aptasensor with Bi-Sb alloy materials (Bi-Sb AMs) was developed for ultrasensitive detection of HER-2, a prognostic biomarker for breast cancer. The aptasensor performance was greatly enhanced by the introduction of high conductivity Bi-Sb AMs. Integration of the aptasensor with the Sensit Smart U-disk electrochemical analyzer enabled point-of-care testing (POCT) for HER-2, with a wide linear response range and low detection limit.
Article
Chemistry, Analytical
Fei Li, Xuegang Li, Xue Zhou, Pengqi Gong, Yanan Zhang, Yong Zhao, Linh Viet Nguyen, Heike Ebendorff-Heidepriem, Stephen C. Warren-Smith
Summary: A Fabry-Perot interference biosensor based on the Vernier effect is developed for label-free DNA hybridization detection, showing high sensitivity and specificity in refractive index range.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Chen-chen Li, Yue-hong Dong, Xiaoran Zou, Xiliang Luo, Dazhong Shen, Juan Hu, Chun-yang Zhang
Summary: A label-free and template-free chemiluminescent biosensor, termed hmC-GLIB-IAS, has been developed for sensitive detection of 5hmC in genomic DNA. This method shows advantages of bisulfite-free, improved sensitivity, and genome-wide analysis of 5hmC at constant reaction temperature. It can detect 5hmC with a high reproducibility and accuracy without isotope-labeled substrates or specific antibodies.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Rokhsareh Abedi, Jahan Bakhsh Raoof, Ayemeh Bagheri Hashkavayi, Maryam Asghary
Summary: The label-free electrochemical DNA hybridization biosensor developed based on gold nanocubes modified graphite screen-printed electrodes is able to effectively detect the interaction between epirubicin and short sequence DNA of prostate cancer gene, with a linear detection range from 0.04 to 20.0 mu M and a detection limit of 0.01 mu M. The biosensor exhibits good selectivity and demonstrated applicability in human serum samples, serving as a reproducible and stable device for EPI determination.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Narges Asefifeyzabadi, Grace Durocher, Kizito-Tshitoko Tshilenge, Tanimul Alam, Lisa M. Ellerby, Mohtashim H. Shamsi
Summary: We have developed a PNA microprobe sensing platform that utilizes electrochemical impedance spectroscopy to detect trinucleotide repeat mutation. The platform successfully identified Huntington's disease-associated CAG repeats in cell-derived total RNA with a signal-to-noise ratio of 1:3. This sensitive, label-free, and PCR-free detection strategy shows potential for the development of biosensing platforms for various repeat expansion disorders.
Article
Nanoscience & Nanotechnology
Julija Dronina, Deivis Plausinaitis, Urte Samukaite-Bubniene, Arunas Ramanavicius
Summary: Immobilization of DNA-modifying enzymes on surfaces is a complex task in biotechnology and biosensorics. This study focuses on the real-time monitoring of T7 DNA polymerase immobilization and its analytical signals using Quartz crystal micro-balance (QCM). The research evaluates the covalent attachment of T7 DNA polymerase to self-assembled monolayers and proposes a strategy based on protecting the exonuclease active site. The findings suggest the detection of low concentrations of target DNA molecules and efficient reuse of expensive DNA enzymes.
MATERIALS TODAY NANO
(2022)
Article
Chemistry, Multidisciplinary
Tanushree Mana, Jayanta Kundu, Hiya Lahiri, Sudipta Bera, Jayeeta Kolay, Surajit Sinha, Rupa Mukhopadhyay
Summary: Nucleic acid-based biosensors with nucleic acid capture probes offer interesting ways of obtaining clinically relevant information. Peptide nucleic acid (PNA) and morpholino (MO) probes, with their non-ionic backbones, show promising performance in terms of stability and nuclease resistance. In this study, the molecularly resolved nucleic acid sensing capacities of PNA and MO probes were compared to DNA, and it was found that both PNA and MO outperformed DNA in single nucleobase mismatch discrimination, with MO performing the best. The more rigid backbone of MO compared to PNA was suggested to be advantageous for probe orientation and surface accessibility.
Article
Chemistry, Multidisciplinary
Teresa Crisci, Andrea Patrizia Falanga, Maurizio Casalino, Nicola Borbone, Monica Terracciano, Giovanna Chianese, Mariano Gioffre, Stefano D'Errico, Maria Marzano, Ilaria Rea, Luca De Stefano, Giorgia Oliviero
Summary: Zinc oxide nanowires modified with a peptidic nucleic acid probe show potential for label-free biosensing applications, with the ability to detect specific DNA sequences and tumor markers. The specificity and sensitivity of the sensor platform are demonstrated through electrical measurements, paving the way for future biosensing technologies.
Article
Biochemistry & Molecular Biology
Li Li, Zhengbo Chen
Summary: In this study, a high specificity, flexibility, and simplicity aptasensor strategy was designed using a triblock DNA probe. It was found that the polyA fragment could assemble on the gold electrode surface via polyA instead of traditional Au-S bonds, leading to improved hybridization stability. The electrochemical aptasensor showed good repeatability, stability, and specificity.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Elena Alina Chiticaru, Celina Maria Damian, Luisa Pilan, Mariana Ionita
Summary: This study proposes a simple, label-free, and cost-effective DNA detection platform using screen-printed carbon electrodes modified with reduced graphene oxide and gold nanoparticles. The preparation process and electrochemical properties of the modified electrodes were characterized and confirmed. The introduction of gold nanoparticles onto the electrode surface enhanced surface conductivity and sensitivity in detection.
Article
Biophysics
Ruiyun Zhou, Chen Wang, Yuxin Huang, Kang Huang, Yingli Wang, Wendao Xu, Lijuan Xie, Yibin Ying
Summary: This study investigates the integration of graphene with THz metasurface into a microfluidic cell for sensitive biosensing in liquid environment, demonstrating high sensitivity in detecting DNA sequences of Escherichia coli O157:H7. The results suggest the potential of the proposed graphene-metasurface microfluidic biosensor for label-free biosensing applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Enguang Lv, Yanhong Li, Jiawang Ding, Wei Qin
Summary: The study introduces the concept of magnetically controlled extraction of hydrophilic bioreceptors for bioassays. By preventing the extraction of the peptide into the polymeric membrane doped with an ion exchanger, a potential change can be detected. This detection strategy can be used for pattern recognition of bacteria.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemical Research Methods
Akira Matsumoto, Shigehito Osawa, Takahiro Arai, Yukie Maejima, Hidenori Otsuka, Yuji Miyahara
Summary: The study demonstrates a label-free, enzymatic-free, potentiometric determination of fetuin using boronic acid-based SA-recognition chemistry on a gold electrode. The method shows a micro M-order sensitivity comparable to blood-collected specimens, suggesting potential for developing a cost-effective hemodiagnostic technology with ease of downsizing and mass production.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Chemistry, Analytical
Hiroshi Aoki, Masaki Torimura, Hiroshi Habe
Summary: This study explores a method to discriminate microplastics by analyzing the change in fluorescent properties after adsorption of fluorescent dyes onto microplastic surfaces. Fluorescein and Methylene Blue showed increased fluorescence intensity, while Rhodamine 6G showed negligible changes. This research demonstrates the potential to identify and estimate individual plastic materials by investigating appropriate fluorescent dyes.
ANALYTICAL SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Yukichi Horiguchi, Kevin Barthelmes, Yuji Miyahara, Akira Matsumoto
Summary: The detection and isolation of sialic acid (SA) over-expressed biomolecules remains a focus in cancer research. Boronic acid-based SA-recognition chemistry offers a synthetic molecular basis for such techniques. The derivative 5-Boronopicolinic acid, known for its high SA-binding strength, was immobilized on a gold surface and showed submicron-molar level sensitivity in its interaction with the glycoprotein fetuin implicated in cancers.
Review
Polymer Science
Akira Matsumoto, Siyuan Chen
Summary: The passage discusses the importance of continuous glycemic control in diabetes management and the development of insulin delivery platforms based on phenylboronic acid (PBA), including new PBA derivatives and glucose-responsive gels. Boronic acids (BA) are highlighted for their reversible interactions with biomolecules, offering a unique approach for insulin delivery applications.
Article
Materials Science, Multidisciplinary
Wenqian Yang, Takuya Miyazaki, Pengwen Chen, Taehun Hong, Mitsuru Naito, Yuji Miyahara, Akira Matsumoto, Kazunori Kataoka, Kanjiro Miyata, Horacio Cabral
Summary: RNA interference using small interfering RNAs is a promising therapeutic approach, but successful implementation depends on efficient delivery technologies. Research has shown that the flexibility of block catiomers plays a crucial role in constructing effective carriers and achieving efficient gene knockdown.
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Kazunori Igarashi, Horacio Cabral, Taehun Hong, Yasutaka Anraku, Fotios Mpekris, Triantafyllos Stylianopoulos, Thahomina Khan, Akira Matsumoto, Kazunori Kataoka, Yu Matsumoto, Tatsuya Yamasoba
Summary: Recent research has highlighted the potential of dynamic bursting in tumor vasculature as a novel route for drug delivery, allowing for the transportation of sub-100 nm nanoparticles into tumors and possibly affecting the access of other blood-borne particles. Mathematical modeling and experimental comparisons have revealed the characteristics and sizes of burst vents in tumor vasculature, as well as different types of therapeutic drugs and cells that can utilize this phenomenon for transportation.
Article
Materials Science, Biomaterials
Taehun Hong, Takuya Miyazaki, Akira Matsumoto, Kyoko Koji, Yuji Miyahara, Yasutaka Anraku, Horacio Cabral
Summary: Phosphorylcholine (PC) can function as a ligand for targeting cancer cells, enhancing the internalization of biomaterials in pancreatic cancer cells. Enhanced cellular uptake is mediated by energy-dependent active transport through PLTP.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Chemistry, Analytical
Hideki Fujisaki, Tongchatra Watcharawittayakul, Akira Matsumoto, Yuji Miyahara, Tatsuro Goda
Summary: A simple two-step surface modification method for PEDOT:PSS films enables continuous glucose monitoring for biosensing applications. This cost-effective approach covers a dynamic range of physiologically relevant blood glucose levels, making it suitable for large-scale production of biosensors. The proposed technique also allows for the introduction of various ligands and receptors onto commercial PEDOT:PSS films, indicating potential applications in wearable and implantable biosensors.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Engineering, Biomedical
Hiroaki Hatano, Fanlu Meng, Momoko Sakata, Akira Matsumoto, Kazuhiko Ishihara, Yuji Miyahara, Tatsuro Goda
Summary: This study demonstrates the energy independent permeation of a water-soluble phospholipid-mimicking random copolymer through epithelial barriers, as well as the successful transepithelial delivery of insulin while maintaining its bioactivity. These findings are significant for the development of efficient and safe transepithelial drug delivery systems.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Analytical
Hiroshi Aoki
Summary: A novel technique for microscopic observation of single microplastic particles stained with fluorescent dyes has been developed, enabling fluorescence-based determination of the materials of these particles as a first screening of material determination. Commercially available microplastic particles and fluorescent dyes were used to observe the degree of fluorescent intensity for various combinations, revealing statistically significant differences in fluorescent intensity for different materials.
Article
Materials Science, Multidisciplinary
Hajime Fujita, Meiting Hao, Shinji Takeoka, Yuji Miyahara, Tatsuro Goda, Toshinori Fujie
Summary: Paper electronics are a game-changer in next-generation flexible electronics. However, their poor performance has limited their practical implementation. This study reports a high-performance paper-based wearable ammonia sensor with high sensitivity and high tolerance for humidity.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Materials Science, Multidisciplinary
Hideki Fujisaki, Akira Matsumoto, Yuji Miyahara, Tatsuro Goda
Summary: In this study, a chemical derivatization method was used to introduce 6-carboxypyridine-3-boronic acid into PEDOT:PSS films, enabling specific detection of N-acetylneuraminic acid. Modified PEDOT:PSS electrodes showed sensitive and reliable responses to sialic acid in a physiologically relevant concentration range, even in the presence of fetal bovine serum. These findings demonstrate the potential application of chemically modified PEDOT:PSS thin films in sialic acid clinical diagnostics.
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
(2022)
Article
Polymer Science
Siyuan Chen, Takuya Miyazaki, Michiko Itoh, Hiroko Matsumoto, Yuki Moro-Oka, Miyako Tanaka, Yuji Miyahara, Takayoshi Suganami, Akira Matsumoto
Summary: This article describes a novel polyvinyl alcohol-coated microneedle patch with an interconnected porous gel drug reservoir for enhanced skin penetration efficiency and mechanical strength. Compared to other microneedle patches, this new type of patch has stronger skin penetration ability and can achieve on-demand, long-acting transdermal insulin delivery.
Article
Nanoscience & Nanotechnology
Takuya Miyazaki, Thahomina Khan, Yoshihiro Tachihara, Michiko Itoh, Taiki Miyazawa, Takayoshi Suganami, Yuji Miyahara, Horacio Cabral, Akira Matsumoto
Summary: The study demonstrates that boronic acid derivatives can effectively target multiple CSC subpopulations in the hypoxic tumoral microenvironment, providing a potential solution to the challenges faced in cancer chemotherapy.
ACS APPLIED BIO MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Miyuki Tabata, Chiho Kataoka-Hamai, Kozue Nogami, Daiju Tsuya, Tatsuro Goda, Akira Matsumoto, Yuji Miyahara
Summary: A solid-state potentiometric biosensor based on mixed phase modification of a silver surface was proposed in this study, achieving stable electromotive force and biomolecule functionalization simultaneously. The modified surface reduced nonspecific binding of interfering biomolecules and maintained stability under constant chloride ion concentrations. With a low detection limit of 0.1 pM for microRNA 146, this biosensor could be useful as a disposable single-use sensor in medical fields.