Journal
SENSORS
Volume 12, Issue 3, Pages 2400-2413Publisher
MDPI AG
DOI: 10.3390/s120302400
Keywords
electroosmotic flow; cell lysis; dielectrophoresis force; cell manipulation
Funding
- National Science Council of Taiwan under the NSC [NSC 96-2313-B-415 -002 -MY3]
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This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 mu m/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.
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