Article
Microbiology
Li Feng, Mingcheng Zhang, Zhiyi Fan
Summary: This study comprehensively elucidates the population genomics of ST15 Klebsiella pneumoniae (Kpn) in China by analyzing 287 publicly available genomes. The study reveals a sharp increase in the proportion of Kpn genomes from 2012 to 2021, with the majority collected from the Yangtze River Delta region. Carbapenemase genes were detected in a high percentage of the genomes, and a significant proportion of the strains were multidrug resistant and hypervirulent. Phylogenetic analysis identified four clades and transmission clustering showed the high transmissibility of one particular clade. Plasmid profiling revealed heterogeneity in plasmid clusters, with a significant number carrying antimicrobial resistance genes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Cell Biology
Beatriz Beamud, Neris Garcia-Gonzalez, Mar Gomez-Ortega, Fernando Gonzalez-Candelas, Pilar Domingo-Calap, Rafael Sanjuan
Summary: Bacteriophages play key roles in bacterial ecology and evolution and are potential antimicrobials. However, the determinants of phage-host specificity remain elusive. In this study, 46 phages were isolated and tested against 138 clinical isolates of Klebsiella pneumoniae, revealing a narrow host range for most phages. Bacterial capsule diversity was found to be the main factor limiting phage host range, while phage-encoded depolymerases and their sequence types were associated with the ability to infect specific capsular types.
Article
Microbiology
Ziyao Li, Xinmeng Liu, Zichen Lei, Chen Li, Feilong Zhang, Yongli Wu, Xinrui Yang, Jiankang Zhao, Yulin Zhang, Yanning Hu, Fangfang Shen, Pingbang Wang, Junwen Yang, Yulei Liu, Binghuai Lu
Summary: In this study, 662 nonduplicate CRKP strains were collected from across China to identify carbapenemase and polymyxin resistance genes and epidemiological features. The study revealed that mgrB inactivation is the main mechanism of polymyxin resistance in PR-CRKP strains, and its deletion or splicing mutations are associated with ST11 and KL47. Various mutations of the ramR gene were also identified. This study provides important insights into antibiotic resistance in China.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Deyan Donchev, Ivan N. N. Ivanov, Ivan Stoikov, Stefana Sabtcheva, Yordan Kalchev, Marianna Murdjeva, Elina Dobreva, Rumyana Hristova
Summary: The study improved and extended the multi-locus VNTR analysis (MLVA) for strain-level differentiation of Klebsiella pneumoniae species complex. The modified scheme (MLVA8+) showed high concordance with classic MLST and provided greater differentiation capability. MLVA8+ also demonstrated its applicability in investigating the intra-hospital spread of carbapenem-resistant producers. It offers improved typing accuracy and extended typing scope for Klebsiella pneumoniae.
Article
Microbiology
Xianzhen Wei, Qiuxiang Li, Yu He, Linlin Li, Shan Li, Taijie Li
Summary: This study investigated the molecular epidemiology and antimicrobial resistance profiles of carbapenem resistant Klebsiella pneumoniae isolates. The results showed that the isolates were resistant to most common antibiotics and mainly carried metallo-beta-lactamase, with ST11 being the major type.
Article
Immunology
Peng Lan, Rushuang Yan, Ye Lu, Dongdong Zhao, Qiucheng Shi, Yan Jiang, Yunsong Yu, Jiancang Zhou
Summary: Siderophores are low molecular weight compounds that facilitate bacterial iron consumption, with genetic diversity of siderophore-encoding loci contributing to production and regulation complexity in K. pneumoniae. Analysis of three acquired siderophores in 272 K. pneumoniae strains revealed distinct predominant siderophore loci in hypervirulent and multidrug-resistant lineages. Strains producing multiple siderophore types tend to have higher mucoviscosity levels, indicating a possible synergistic interaction between siderophore production and hypermucoviscosity phenotype.
MICROBIAL PATHOGENESIS
(2021)
Article
Microbiology
Xiangyun Wu, Jiayi Liu, Jiawei Feng, Muhammad Abu Bakr Shabbir, Yali Feng, Rui Guo, Meifang Zhou, Sulin Hou, Guiqiang Wang, Haihong Hao, Guyue Cheng, Yulian Wang
Summary: This study investigated the antimicrobial resistance pattern and virulence profile of K. pneumoniae in five dairy farms in Hubei, China. The results showed that K. pneumoniae has epidemiological importance in food-producing animals and the emergence of multidrug-resistant K. pneumoniae poses a potential threat to food safety and public health.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Einas A. Osman, Nagwa E. El-Amin, Leena L. Al-Hassan, Maowia Mukhtar
Summary: The study investigated the molecular epidemiology of Klebsiella pneumoniae in major regional hospitals in Khartoum, Sudan, revealing 52 different sequence types and emphasizing the urgent need for systematic epidemiological studies on drug-resistant infections.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Biochemistry & Molecular Biology
Jane Hawkey, Ben Vezina, Jonathan M. Monk, Louise M. Judd, Taylor Harshegyi, Sebastian Lopez-Fernandez, Carla Rodrigues, Sylvain Brisse, Kathryn E. Holt, Kelly L. Wyres
Summary: KpSC is a group of Klebsiella bacteria that can cause opportunistic healthcare-associated infections in humans. Researchers generated strain-specific genome-scale metabolic models (GEMs) for 37 isolates, representing all seven taxa. These models revealed strain-specific differences within and between species.
Article
Infectious Diseases
Lin Huang, Min Cao, Yanyan Hu, Rong Zhang, Yufei Xiao, Gongxiang Chen
Summary: The trend of fosfomycin resistance among KPC-KP strains is increasing in China, with the two major mechanisms of resistance being plasmid-mediated fosfomycin resistance gene fosA3 and mutation of the target gene glpT.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Microbiology
Zhiwei Zheng, Lizhang Liu, Lianwei Ye, Yating Xu, Sheng Chen
Summary: A nationwide survey in China identified seven blaIMP-4-carrying CRKP isolates, which exhibited MDR and were linked to different regions. Genetic analysis revealed significant variation among these CRKP strains and identified multiple resistance factors. This highlights the need for expanded surveillance and control measures to prevent dissemination of CRKP strains and improve antibiotic stewardship and infection control.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Microbiology
Luis Janssen, Felipe Marques de Almeida, Thais Amanda Silva Damasceno, Rodrigo de Paula Baptista, Georgios Joannis Pappas, Tatiana Amabile de Campos, Vicente de Paulo Martins
Summary: Antimicrobial resistance is a growing concern globally, with the discovery of multidrug resistant bacteria in a Brazilian urban lake pointing to potential environmental sources of resistance.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Jiao Liu, Zhuofei Xu, Haibo Li, Fuhui Chen, Kaiyu Han, Xiaoman Hu, Yuan Fang, Dechang Chen
Summary: In this study, metagenomic next-generation sequencing was used to analyze the genome biology of Klebsiella pneumoniae prevalent in China. Strain-level profiling and analyses of antimicrobial resistance and virulence genes were performed. The study highlights the advantages of strain-level metagenomics in understanding the resistance and virulence mechanisms of K. pneumoniae directly from clinical specimens.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Sanjay Singh, Ashutosh Pathak, Mohibur Rahman, Avinash Singh, Soumyabrata Nag, Chinmoy Sahu, Kashi Nath Prasad
Summary: The study identified that colistin resistance in Klebsiella pneumoniae is mainly mediated by mcr-1. The co-existence of colistin, carbapenem, and other drug-resistant genes along with efflux pumps suggests significant genomic plasticity in K. pneumoniae, potentially leading to the emergence of super-spreaders of drug resistance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Yanzhu Chen, Yi Chen
Summary: Hypervirulent Klebsiella pneumoniae (hvKP) is a pathogen with higher virulence than classical strains, posing a significant threat to public health in China. The country faces the urgent task of identifying hvKP and providing optimal treatment.
JOURNAL OF TRANSLATIONAL INTERNAL MEDICINE
(2021)
Article
Toxicology
Duo Su, Changjiao Gan, Zhouguang Jiao, Mengyun Deng, Sha Li, Yingjiao Ju, Yefeng Qiu, Lingfei Hu, Bo Gao, Dongsheng Zhou, Yuee Zhao, Huiying Yang
Summary: BoNT/A, known for its medical and cosmetic applications, can also be used as a potential biological weapon. Studies have shown that it affects nonneuronal cells and induces global gene expression alterations in lung tissues. The research reveals a transcriptional shift towards a pro-reparative phenotype in response to BoNT/A inhalation, indicating its potential therapeutic application in mitigating inflammatory lung conditions.
JOURNAL OF APPLIED TOXICOLOGY
(2021)
Article
Microbiology
Hongchao Chen, Zhe Zhan, Xiaoyuan Jiang, Yang Qing, Zhe Yin, Lingling Mei, Dongsheng Zhou, Bin Ni, Yanjun Zhang
Summary: This study found that plasmids with single Inc(pA1763-KPC) replicons are less studied despite their emergence in some strains. Through genome sequencing and comparison, it was discovered that plasmids pA1763-KPC and p427113-2 from two clinical Klebsiella pneumoniae isolates showed different backbone structures compared to six previously sequenced representative Inc(pA1763-KPC) plasmids.
JOURNAL OF BASIC MICROBIOLOGY
(2021)
Article
Toxicology
Zhouguang Jiao, Sha Li, Duo Su, Changjiao Gan, Xiaodong Zhao, Bo Gao, Yefeng Qiu, Yajun Song, Dongsheng Zhou, Lingfei Hu, Huiying Yang
Summary: This study established a ricin toxin-induced pulmonary intoxication model in mice, and found that exposure to ricin toxin led to inflammation and cell damage in the lungs, with poorly studied genes potentially serving as important targets for future research.
TOXICOLOGY LETTERS
(2021)
Article
Gastroenterology & Hepatology
Yiquan Zhang, Yue Qiu, Xingfan Xue, Miaomiao Zhang, Junfang Sun, Xue Li, Lingfei Hu, Zhe Yin, Wenhui Yang, Renfei Lu, Dongsheng Zhou
Summary: QsvR directly repressed the transcription of toxR and calR while activating the transcription of cpsQ-mfpABC and mfpABC. This study provides deeper insights into the regulatory network of the mfp locus in Vibrio parahaemolyticus.
Article
Chemistry, Multidisciplinary
Qingyuan Wu, Fengrong Zhang, Xueting Pan, Zhijun Huang, Zhijie Zeng, Hongyu Wang, Jun Jiao, Xiaolu Xiong, Lixin Bai, Dongsheng Zhou, Huiyu Liu
Summary: Sonodynamic therapy (SDT) enhanced by nanoparticle wettability has been investigated in this study, revealing that the desorption energy of nanoparticles, determined by their wettability, positively correlates with the stability of nanobubbles and the efficiency of SDT. Experimental results on thrombus models further demonstrate that nanoparticles with the largest desorption energy exhibit the highest thrombolytic efficiency.
ADVANCED MATERIALS
(2021)
Article
Immunology
Ting Yu, Huiying Yang, Jun Li, Fangzhou Chen, Lingfei Hu, Ying Jing, Xinhua Luo, Zhe Yin, Mingxiang Zou, Dongsheng Zhou
Summary: This study fully sequenced the genomes of nine clinical P. aeruginosa isolates, identifying multiple genetic elements related to antibiotic resistance. Additionally, 20 novel mobile genetic elements (MGEs) were identified for the first time, including new variants of resistance genes and a novel isolate of P. aeruginosa. The data presented in this study demonstrate that complex transposition and homologous recombination play a key role in the integration of genetic elements into P. aeruginosa chromosomes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Renfei Lu, Junfang Sun, Yue Qiu, Miaomiao Zhang, Xingfan Xue, Xue Li, Wenhui Yang, Dongsheng Zhou, Lingfei Hu, Yiquan Zhang
Summary: This study revealed how quorum sensing regulates swimming motility in Vibrio parahaemolyticus, showing that its master regulator OpaR directly influences the transcription of Pof genes.
JOURNAL OF MICROBIOLOGY
(2021)
Article
Infectious Diseases
Mingxing Huang, Jinmin Ma, Jun Jiao, Chunna Li, Luan Chen, Zhongyi Zhu, Feng Ruan, Li Xing, Xinchun Zheng, Mengjiao Fu, Binyin Ma, Chongjie Gan, Yuanchen Mao, Chongnan Zhang, Ping Sun, Xi Liu, Ziliang Lin, Lu Chen, Zhiyu Lu, Dongsheng Zhou, Bohai Wen, Weijun Chen, Xiaolu Xiong, Jinyu Xia
Summary: This study utilized clinical metagenomic next-generation sequencing (mNGS) and mobile phone location data to confirm an epidemic of Q fever in Zhuhai, China, identifying Cb-infected goats and cattle at the official slaughterhouse as the likely source of infection. This is the first confirmed epidemic of Q fever in a contemporary city in China, showcasing the usefulness of mNGS for rapid and effective public health responses to acute bacterial infections.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Immunology
Xinhua Luo, Zhe Yin, Lijun Zeng, Lingfei Hu, Xiaoyuan Jiang, Ying Jing, Fangzhou Chen, Dongguo Wang, Yajun Song, Huiying Yang, Dongsheng Zhou
Summary: In this study, a detailed genetic dissection of the bla (NDM)-carrying genetic elements and their related mobile genetic elements in Enterobacteriaceae revealed the presence of numerous resistance genes and complex structures. The analysis identified eight novel mobile elements, including ICEs, IMEs, composite transposons, and an integron.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Yiquan Zhang, Yue Qiu, He Gao, Junfang Sun, Xue Li, Miaomiao Zhang, Xingfan Xue, Wenhui Yang, Bin Ni, Lingfei Hu, Zhe Yin, Renfei Lu, Dongsheng Zhou
Summary: The master QS regulator OpaR in Vibrio parahaemolyticus directly regulates a group of genes to impact the concentration of c-di-GMP, which in turn influences behaviors such as biofilm formation. This direct association between QS and c-di-GMP metabolism allows for precise control of gene transcription and bacterial behaviors.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Mengjiao Fu, Jianing Zhang, Mingliang Zhao, Shan Zhang, Lupeng Dai, Xuan Ouyang, Yonghui Yu, Bohai Wen, Dongsheng Zhou, Yansong Sun, Jun Jiao, Xiaolu Xiong
Summary: Coxiella bumetii's CpeB effector protein promotes the accumulation of LC3-II and enhances the virulence of C. burnetii through a pathway involving Rab11a.
INFECTION AND IMMUNITY
(2022)
Article
Engineering, Biomedical
Yunhang Liu, Bolong Xu, Mingzhu Lu, Shanshan Li, Juan Guo, Fangzhou Chen, Xiaolu Xiong, Zhe Yin, Huiyu Liu, Dongsheng Zhou
Summary: Due to increasing antibiotic resistance, alternative antibacterial strategies are urgently needed. This study developed iron-doped carbon dots with photothermal conversion and photoenhanced enzyme-like properties for efficient antibacterial therapy and wound healing. The iron-doped carbon dots killed bacteria by generating heat and reactive oxygen species, and promoted wound healing through preventing infection, enhancing tissue proliferation, and inducing angiogenesis. This study is of great importance for finding new antibacterial approaches.
BIOACTIVE MATERIALS
(2022)
Article
Microbiology
Qimin Wu, Xue Li, Tingting Zhang, Miaomiao Zhang, Xingfan Xue, Wenhui Yang, Lingfei Hu, Zhe Yin, Dongsheng Zhou, Yuyu Sun, Renfei Lu, Yiquan Zhang
Summary: Vibrio parahaemolyticus can naturally and reversibly switch between wrinkly and smooth phenotypes, and this process is strictly regulated by a complex molecular regulatory network. Significantly differentially expressed genes are involved in various biological behaviors during this process.
MICROBIOLOGY SPECTRUM
(2022)
Article
Pharmacology & Pharmacy
Menghuan Zhu, Dongna Zhang, Lili Zhang, Liangliang Zhao, Likun Xu, Baogang Wang, Xinyu Zhang, Jinwei Chen, Zhuchun Bei, Hong Wang, Dongsheng Zhou, Wenhui Yang, Yabin Song
Summary: In this study, GM powders were prepared using spray-drying technology and the formulation was optimized. The results suggest that inhalation therapy with GM spray-dried powders is an effective treatment for pneumonic plague.
Article
Microbiology
Ying Jing, Zhe Yin, Peng Wang, Jiayao Guan, Fangzhou Chen, Lingling Wang, Xinyue Li, Xiaofei Mu, Dongsheng Zhou
Summary: This study provides a comprehensive genomic epidemiology analysis of global Morganella isolates. A new genome sequence-based species classification scheme for Morganella is established, which shows higher resolution and accuracy compared to the conventional scheme. The prevalence of accessory genetic elements (AGEs) and associated antimicrobial resistance genes (ARGs) in Morganella isolates is revealed based on genome sequences. The study also uncovers the diverse evolution of Morganella chromosomes in acquiring AGEs harboring different profiles of ARGs, some of which contain large accessory modules with complex mosaic structures. The findings deepen the understanding of the classification and evolution of Morganella species and the role of ARG-containing AGEs in Morganella at the genomic scale.
MICROBIOLOGY SPECTRUM
(2022)
