Article
Chemistry, Multidisciplinary
Ngoc Duc Vo, Anh Thi Van Nguyen, Hoi Thi Le, Nam Hoang Nguyen, Huong Thi Thu Pham
Summary: The study developed an alternative method, the MACS-Countess system, for CD4(+) T cell counting, which combines magnetic activated cell sorting with an automated cell counting system. Compared to the FACS gold standard, the MACS-Countess system showed a mean bias of 3.5% with a narrow limit of agreement, making it a reliable and cost-effective option for laboratories in limited resource regions.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Nastaran Moradi, Samad Muhammadnejad, Hamid Delavari, Negin Pournoori, Mohammad Ali Oghabian, Hossein Ghafouri
Summary: The conjugation of anti-CD3 monoclonal antibody with Fe3O4 coated by carboxymethyl dextran (CMD) using cyanogen bromide (CNBr) was shown to efficiently separate CD3+ T lymphocytes from whole blood with high purity. The method can be utilized for cell sorting and targeted MRI imaging in the future.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemical Research Methods
Nicklas H. Staunstrup, Charlotte C. Petersen, Tina Fuglsang, Anna Starnawska, Ana Chernomorchenko, Per Qvist, Vivien R. Schack
Summary: This study compared two fluorescence-activated technologies for cell sorting from small input samples, finding that the mechanical valve-based Tyto instrument perturbed cells to a lesser extent and resulted in significantly higher proliferation potential of T-cells compared to the high pressure and electrostatic deflection-based Aria instrument.
Article
Cell Biology
Cristina Gonzalez-Ravina, Esther Santamaria-Lopez, Alberto Pacheco, Julia Ramos, Francisco Carranza, Lucia Murria, Ana Ortiz-Vallecillo, Manuel Fernandez-Sanchez
Summary: This study analyzed the clinical outcomes of magnetic-activated cell sorting (MACS) on donor intrauterine insemination (D-IUI) cycles. The results showed no significant differences in gestation, live birth, or miscarriage rates between the MACS group and the control group. Further studies with a larger sample size are needed to evaluate the application of MACS in fertility clinics.
Article
Materials Science, Multidisciplinary
Matthew Lindley, Julia Gala de Pablo, Jorgen Walker Peterson, Akihiro Isozaki, Kotaro Hiramatsu, Keisuke Goda
Summary: Cell sorting is an important tool in biological research and medicine. Raman-activated cell sorting (RACS) has gained attention for its ability to discriminate cells based on their intracellular chemical content without the need for labeling. However, the widespread use of RACS has been limited by a trade-off between throughput and measurement bandwidth. In this study, this trade-off is overcome and broadband RACS in the fingerprint region with a record high throughput is demonstrated. Real-time label-free sorting of microalgal cells based on their accumulation of carotenoids and polysaccharide granules is also showcased.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Multidisciplinary Sciences
Eija Maki-Mikola, Patrick Lauren, Natsumi Uema, Kanako Kikuchi, Yuuki Takashima, Timo Laaksonen, Tatu Lajunen
Summary: This study established a perfusion cell culture platform to investigate the effects of flow on the uptake of light-activated liposomes by A549 lung cancer cells. Optimal cell culturing parameters were determined by monitoring cell viability, and flow cytometry was used to measure particle uptake for determining optimal liposome treatment times. The suitability of commercial QuasiVivo flow-chambers for light activation studies was assessed.
SCIENTIFIC REPORTS
(2023)
Article
Energy & Fuels
F. Sessa, G. Merlin, P. Canu
Summary: The exploitation of pine bark for production of biochar or activated carbons is influenced by the maximum temperature. Chemical activation of activated carbons has shown significant effectiveness in VOC capture, with the one activated by K2CO3 showing the highest loading capacity.
Article
Biology
Korawan Sringarm, Marninphan Thongkham, Supamit Mekchay, Chompunut Lumsangkul, Wannaluk Thaworn, Wiwat Pattanawong, Ekaphot Rangabpit, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Anucha Sathanawongs, Surat Hongsibsong
Summary: This study evaluates the efficiency of bovine sperm sexing using magnetic-activated cell sorting (MACS) by scFv antibody against Y-chromosome-bearing sperm (Y-scFv) coupled to magnetic microbeads and its effects on kinematic variables, sperm quality, and X/Y-sperm ratio. The results show that PY-microbeads are highly effective in separating Y-sperm from X-sperm with no negative impact on the quality of X-sperm. The technique produces high percentages of X-sperm and Y-sperm, making it a feasible method for bovine sperm sexing.
Article
Biochemical Research Methods
Yaqi Zhao, Akihiro Isozaki, Maik Herbig, Mika Hayashi, Kotaro Hiramatsu, Sota Yamazaki, Naoko Kondo, Shinsuke Ohnuki, Yoshikazu Ohya, Nao Nitta, Keisuke Goda
Summary: Intelligent image-activated cell sorting (iIACS) utilizes artificial intelligence (AI) algorithms for high-throughput image-based sorting of cells, solving the issue of flow speed fluctuation by predicting the arrival time of cells at the sort point and activating sorting accordingly. The accuracy of sort-timing prediction is improved through machine learning techniques.
Review
Biochemistry & Molecular Biology
Giovanni Schepici, Agnese Gugliandolo, Emanuela Mazzon
Summary: Mesenchymal stem/stromal cells (MSCs) are undifferentiated cells known for their immunomodulatory and regenerative properties. The use of fetal bovine serum (FBS) in culture media poses limitations due to ethical and safety concerns. Serum-free cultures supplemented with growth factors or chemicals can enhance the proliferation and neuronal differentiation of MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Christin Starzonek, Mouna Mhamdi-Ghodbani, Stefan Henning, Marc Bender, Sarah Degenhardt, I-Peng Chen, Mohamed Said, Ruediger Greinert, Beate Volkmer
Summary: Highly purified DSCs with good recovery rate can be obtained through positive selection with MACS(R) immunomagnetic cell sorting, which provides a novel and unique model for studying the effects of UV radiation on the differentiation of DSCs into melanocytes and their potential relevance in the genesis of malignant melanoma.
Review
Biochemistry & Molecular Biology
Shogo Ichii, Izumi Matsuoka, Fumiyoshi Okazaki, Yasuhito Shimada
Summary: Human life expectancy has increased, leading to a rising proportion of elderly individuals and the associated issue of skeletal muscle aging. Exercise therapy is currently the only treatment option for this condition. This review explores strategies for modeling skeletal muscle aging in cell cultures and rodents, and discusses the potential of zebrafish models for future research.
Article
Andrology
Minpeng Ji, Dan Chen, Xingyi Zhao, Fu Huang, Xiaoju Guan, Xin Wen, Jiexia Wang, Jingjing Shao, Jiajia Xie, Dan Shan, Shuyan Cao, Congde Chen, Haolin Chen
Summary: This study describes a new method to isolate LCs from rat testes in large quantity with high purity and viability. The purified LCs retain their steroidogenic and non-steroidogenic functions, making them valuable for studying the physiology, pharmacology, and toxicology of the cells in vitro.
Article
Biophysics
Rui Tang, Lin Xia, Bien Gutierrez, Ivan Gagne, Adonary Munoz, Korina Eribez, Nicole Jagnandan, Xinyu Chen, Zunming Zhang, Lauren Waller, William Alaynick, Sung Hwan Cho, Cheolhong An, Yu-Hwa Lo
Summary: Classification and sorting of cells using image-activated cell sorting (IACS) systems can provide valuable insights to biomedical sciences. However, the limited capabilities and complex implementation of deep learning-assisted IACS systems have hindered their widespread adoption in biomedical research. In this study, the authors present a label-free cell sorting solution based on real-time AI inferencing and fast training of a deep learning model. The system achieves high sorting purity with a short latency time, setting a new record for IACS with sorting by AI inference.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Biotechnology & Applied Microbiology
Shuaishuai Yan, Jingxuan Qiu, Liang Guo, Dezhi Li, Dongpo Xu, Qing Liu
Summary: Understanding metabolic interactions between bacteria at the single-cell level and the contribution of individual cells is crucial for exploring uncultured bacteria. The combination of RACS and SCRS with unique fingerprint characteristics has allowed microbiology research to enter the single-cell era. This review summarizes current research progress on recognizing and assessing single bacterial cells based on RACS, highlighting the importance of RACS platforms in linking cell genotype and phenotype, as well as methods for distinguishing single-cell phenotype.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Behavioral Sciences
Nick A. R. Jones, Mike Webster, Cait Newport, Christopher N. Templeton, Stefan Schuster, Luke Rendell
Article
Multidisciplinary Sciences
Alexander Hecker, Wolfram Schulze, Jakob Oster, David O. Richter, Stefan Schuster
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biology
Alexander Hecker, Pamela Anger, Philipp N. Braaker, Wolfram Schulze, Stefan Schuster
COMMUNICATIONS BIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Yijiang Huang, Daniel Seitz, Yan Chevalier, Peter E. Mueller, Volkmar Jansson, Roland M. Klar
Article
Nanoscience & Nanotechnology
Felix Blendinger, Daniel Seitz, Andreas Ottenschlager, Monika Fleischer, Volker Bucher
Summary: TiO2 thin films deposited on PEEK using PEALD method show promising osteogenic properties, enhancing the bioactivity of the implants effectively.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biology
Georg Welzel, Stefan Schuster
Summary: The research found that African electric catfish are highly protected against high-voltage shocks, even resisting the effects of commercial electrofishing devices. The nature of this shielding mechanism is currently unclear.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Biology
Peggy Gerullis, Caroline P. Reinel, Stefan Schuster
Summary: Archerfish use water jets to shoot down aerial prey from various distances, adjusting the jets according to the size and distance of the prey. The rapid fin maneuvers, especially of the pectoral and pelvic fins, are coordinated with the release of the water jet. These fin maneuvers are essential for stabilizing the shooter and are crucial for the precise and powerful shooting abilities of archerfish.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Biology
Elisabeth Schirmer, Stefan Schuster, Peter Machnik
Summary: Bisphenols, important plasticizers released into the biosphere, have been found to have alarming and uncompensated effects on neuronal functioning in mature vertebrate brains. The study calls for the rapid development of alternative plasticizers and presents a new assay method to accelerate early testing phases for next-generation plasticizers.
COMMUNICATIONS BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Peter Machnik, Stefan Schuster
Summary: The article highlights the increasing use of chemicals in modern societies and the importance of rigorous risk assessment before approving new substances for commerce. It suggests a fast and straightforward method to detect the effects of chemical exposure on brain function by recording from a single neuron in fish, which can provide valuable information for risk assessment.
Article
Biology
Georg Welzel, Stefan Schuster
Summary: Gap junction channels are formed by two unrelated protein families, with non-chordates using innexins and chordates using connexins. Surprisingly, glycosylated innexins are not unique to chordates, as they are also found in non-chordates. The loss of innexin diversity during early chordate evolution and the presence of only one glycosylated innexin in lancelets, which lack connexins, suggest a bottleneck effect and explain why connexins have become the exclusive protein for chordate gap junctions.
Article
Biology
Georg Welzel, Stefan Schuster
Summary: The electric catfish has a vulnerable heart that is sensitive to external electrical currents and shock-induced arrhythmias. However, it lacks a specialized cardiac conduction system, and its heart requires efficient but unknown means of protection.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2022)
Article
Biology
Peter Machnik, Nastaran Biazar, Stefan Schuster
Summary: This study used intracellular in vivo recordings in the Mauthner neuron of goldfish to determine the effects of isoeugenol on sensory and neural function. The research found that isoeugenol strongly affects hearing and vision, with significant differences in sensitivity and time course of action. The study also showed that isoeugenol is highly useful for fish anesthesia during handling, but its application needs to be carefully adapted for more severe treatment.
COMMUNICATIONS BIOLOGY
(2023)
Review
Behavioral Sciences
Stefan Schuster
Summary: A quick decision allows hunting archerfish to secure prey even when outnumbered by competing fish. The fish select a rapid start based on brief information about prey motion and turn towards the catch point. These start decisions are indistinguishable from escape starts and enable the fish to accurately respond to various prey movements and fish positions. The start decisions do not sacrifice speed for accuracy and are resistant to environmental changes.
JOURNAL OF COMPARATIVE PHYSIOLOGY A-NEUROETHOLOGY SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY
(2023)
