Detection of E.Coli Bacteria Using Impedance Spectroscopy and Surface Plasmon Resonance Imaging Based Biosensor

In this work, we report a biosensing approach based on immunodetection of E.coli bacteria. Two different transducers were used for quantitative analysis of antibody-bacteria interaction. Two types of antibodies were immobilized on gold surface by simple physisorption technique. Electrochemical impedance spectroscopy was used to characterize each step of biosensor conception and SPA imaging for bacteria detection. Impedimetric and optical generated signals were tested against E.coli bacteria. The detection limit of 10(4) CFU/ml was reached for impedimetric biosensor and 10(5) CFU/ml for SPR based biosensor.