Review
Engineering, Biomedical
Zheng Xing, Chen Zhao, Siwen Wu, Chunchen Zhang, Haifeng Liu, Yubo Fan
Summary: Therapeutic angiogenesis provides a potential solution for critical limb ischemia by manipulating blood vessel growth in vivo without the need for extra pharmaceutics and surgery. With the rise of interdisciplinary research, regenerative medicine strategies offer new possibilities for treating various clinical diseases.
Article
Peripheral Vascular Disease
Yuuki Shimizu, Kazuhisa Kondo, Ryo Hayashida, Ken-ichiro Sasaki, Masanori Ohtsuka, Yoshihiro Fukumoto, Shinichiro Takashima, Oto Inoue, Soichiro Usui, Masayuki Takamura, Masashi Sakuma, Teruo Inoue, Tokuichiro Nagata, Yoshihiro J. Akashi, Yoshihiro Yamada, Tamon Kato, Koichiro Kuwahara, Kaoru Tateno, Yoshio Kobayashi, Rei Shibata, Toyoaki Murohara
Summary: This multicenter clinical trial demonstrated that autologous adipose-derived regenerative cell (ADRC) transplantation is safe and effective for therapeutic angiogenesis in patients with critical limb ischemia (CLI). The overall survival rate was 100% at the post-operative period and 6 months follow-up. 94.1% of limbs were free from major amputation for 6 months. The quality of life score, ulcer size, and walking distance also improved in a significant number of patients.
Review
Cell & Tissue Engineering
Laura Lozano Navarro, Xueyi Chen, Lady Tatiana Girata Viviescas, Andrea K. Ardila-Roa, Maria L. Luna-Gonzalez, Claudia L. Sossa, Martha L. Arango-Rodriguez
Summary: Peripheral arterial disease affects hundreds of millions of people worldwide and can lead to limb amputation or death. Revascularization is crucial for preventing amputation, but a significant portion of patients are not suitable for traditional surgical treatment, resulting in higher amputation rates. Mesenchymal stem cells have emerged as a promising cell-based therapy for peripheral arterial disease due to their regenerative properties.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Pegah Nammian, Seyedeh-Leili Asadi-Yousefabad, Sajad Daneshi, Mohammad Hasan Sheikhha, Seyed Mohammad Bagher Tabei, Vahid Razban
Summary: The study compared the effects of allogeneic bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue mesenchymal stem cells (AT-MSCs) in the treatment of critical limb ischemia (CLI). The results indicated that BM-MSCs had a stronger effect on endothelial cell migration, muscle restructure, functional improvements, and neovascularization in ischemic tissues compared to AT-MSCs. This suggests that BM-MSC transplantation could be a more effective therapy for diseases with insufficient angiogenesis, such as hindlimb ischemia.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Lucia Beltran-Camacho, Marta Rojas-Torres, Ma Carmen Duran-Ruiz
Summary: Critical limb ischemia is the most severe form of peripheral arterial disease, commonly caused by atherosclerosis leading to progressive blockage of arterial vessels. For patients not suitable for surgical intervention, angiogenic cell therapy has emerged as a promising alternative, showing potential for enhancing revascularization by promoting vessel formation and blood flow recovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Grazia Marsico, Chunseng Jin, Sunny A. Abbah, Eva M. Brauchle, Dilip Thomas, Ana Lucia Rebelo, Doriana Orbanic, Sandrine Chantepie, Paolo Contessotto, Dulce Papy-Garcia, Carlos Rodriguez-Cabello, Michelle Kilcoyne, K. Schenke-Layland, N. G. Karlsson, Karl J. A. McCullagh, Abhay Pandit
Summary: This study demonstrated glycosylation changes associated with ischemia in a murine CLI model, and evaluated the efficacy of an ELR hydrogel for CLI treatment. The ELR hydrogel modulated angiogenic signaling pathways, promoted capillary formation and ECM remodeling, while also inducing arterioles formation, reducing fibrosis, and polarizing anti-inflammatory macrophages. The study suggests a potential role for mannosylation and sialylation in tissue repair, highlighting glycosylation alterations as new therapeutic targets for CLI.
Review
Cell & Tissue Engineering
Zeinab Shirbaghaee, Mohammad Hassani, Saeed Heidari Keshel, Masoud Soleimani
Summary: This article reviews the immunomodulatory features of mesenchymal stem cells (MSCs) and their potential applications as therapeutic agents in the treatment of critical limb ischemia (CLI). Despite advances in clinical research, MSC therapy is still considered experimental and not recommended for regular clinical use. The article provides recommendations to improve the efficacy of cell therapy for CLI patients.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Chemistry, Physical
Liang Gui, Juju Huang, Yi Xing, Yongjun Li, Junjie Zou, Yingwei Zhu, Xiao Liang, Xiwei Zhang, Qiang Xu, Xin Du
Summary: A near-infrared light-driven Cu2+-loaded polydopamine nanomotor was designed, which can promote angiogenesis and improve the pathological micro-environment of ischemic tissue, enhancing the therapeutic effects.
Article
Cardiac & Cardiovascular Systems
Lirong Xu, Yutong Liu, Qianyun Cheng, Yang Shen, Ye Yuan, Xiaolang Jiang, Xu Li, Daqiao Guo, Junhao Jiang, Changpo Lin
Summary: CLI, the most advanced clinical stage of peripheral vascular disease, leads to lower extremity rest pain, ulceration, and gangrene due to insufficient blood and oxygen supply, making effective biomarkers and therapeutic targets crucial. The study confirmed the downregulation of circadian gene Bmal1 in CLI patients, affecting inflammation and angiogenesis. Bmal1 disruption impairs angiogenesis and endothelial cell functions, suggesting its potential as a biomarker and therapeutic target in CLI.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Engineering, Biomedical
Zuoguan Chen, Jianwei Duan, Yongpeng Diao, Youlu Chen, Xiaoyu Liang, Huiyang Li, Yuqing Miao, Qing Gao, Liang Gui, Xiaoli Wang, Jing Yang, Yongjun Li
Summary: The study developed a zinc-based metal-EGCG capsule, which was shown to significantly promote angiogenesis, improve the microenvironment, and reduce toxicity. In vitro and in vivo experiments demonstrated that EGCG/Zn Ps exhibited antioxidant, anti-inflammatory, and cell migration-inducing effects.
BIOACTIVE MATERIALS
(2021)
Article
Cardiac & Cardiovascular Systems
Lirong Xu, Yutong Liu, Qianyun Cheng, Yang Shen, Ye Yuan, Xiaolang Jiang, Xu Li, Daqiao Guo, Junhao Jiang, Changpo Lin
Summary: CLI, the most advanced stage of peripheral vascular disease, involves downregulation of circadian gene Bmal1 which affects lipid metabolism, inflammation, and angiogenesis. This downregulation impairs angiogenesis and endothelial cell functions, while repressing inflammation and promoting angiogenesis through transcriptional regulation of VEGF. Therefore, Bmal1 may serve as an effective biomarker and therapeutic target in CLI.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Tao Bai, Mingxing Li, Yuanfeng Liu, Zhentao Qiao, Xusheng Zhang, Yafeng Wang, Zhiwei Wang
Summary: The study found that AURKA plays an important role in diabetes-related ischemic limb ischemia, improving vascular regeneration and functional recovery. In addition, AURKA can also inhibit oxidative stress and lipid peroxidation reactions, providing protection against diabetic limb ischemia.
MOLECULAR MEDICINE
(2023)
Review
Genetics & Heredity
Vyacheslav Z. Tarantul, Alexander Gavrilenko
Summary: Peripheral artery diseases are a serious public health problem. This review focuses on gene therapy as an alternative and potentially more effective treatment option for no-option patients. The review presents the results of many years of research and application of gene therapy as a treatment for patients with critical limb ischemia, including successful and unsuccessful attempts. The review also highlights trends in changing approaches to therapeutic angiogenesis.
CURRENT GENE THERAPY
(2022)
Review
Medicine, Research & Experimental
Jingxuan Han, Lailiu Luo, Olivia Marcelina, Vivi Kasim, Shourong Wu
Summary: Peripheral artery disease (PAD) poses a significant challenge to society and its prevalence is expected to increase in the coming years. Patients in severe stages of PAD are at high risk of amputation and death, leading to decreased quality of life and socio-economic burden. Therapeutic angiogenesis has emerged as a potential strategy for treating PAD, but further efforts are needed to address certain issues.
Article
Peripheral Vascular Disease
Michael G. McCoy, Anurag Jamaiyar, Grasiele Sausen, Henry S. Cheng, Daniel Perez-Cremades, Rulin Zhuang, Jingshu Chen, Philip P. Goodney, Mark A. Creager, Marc S. Sabatine, Marc P. Bonaca, Mark W. Feinberg
Summary: Peripheral artery disease (PAD) is a serious condition that can lead to limb loss and death, especially in subjects with diabetes. In this study, researchers identified miR-375 as a potential therapeutic target for critical limb ischemia (CLI), an advanced form of PAD. They found that miR-375, when overexpressed, promotes angiogenesis and arteriogenesis, improving blood flow recovery in diabetic mice. Further investigation revealed that miR-375 regulates the NF-kB signaling pathway by targeting Kruppel-like factor 5 (KLF5). These findings suggest that targeting the miR-375-KLF5-NF-kB signaling axis may be an effective strategy for treating CLI in diabetes.