Review
Hematology
Nikolaus B. Binder, Francois Depasse, Julia Mueller, Thomas Wissel, Stephan Schwers, Matthias Germer, Bjorn Hermes, Peter L. Turecek
Summary: TGA is a global assay that allows for the dynamic and continuous recording of both thrombin generation and inactivation effects. It is widely used in clinical medicine for the diagnosis, prognosis, prophylaxis, and treatment of inherited and acquired bleeding and thrombotic disorders.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Review
Hematology
Francois Depasse, Nikolaus B. Binder, Julia Mueller, Thomas Wissel, Stephan Schwers, Matthias Germer, Bjorn Hermes, Peter L. Turecek
Summary: Thrombin generation assay, also known as thrombin generation test, is a global assay that provides a comprehensive view of hemostasis balance by continuously and simultaneously measuring thrombin formation and inhibition. Standardization and automation of this assay have provided researchers, clinical laboratories, and the pharmaceutical industry with a versatile tool for various clinical and non-clinical applications.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Medicine, General & Internal
Axel Schlagenhauf, Harald Haidl, Georg Trummer, Michael Berchtold-Herz, Jan-Steffen Pooth, Tanja Strini, Ulrich Geisen, Friedhelm Beyersdorf, Barbara Zieger
Summary: This study compared the thrombin generation in the plasma samples of patients using different heart assist devices and found that patients using Heartmate II had poorer thrombin generation within three days after surgery, while patients using Heartmate 3 had better thrombin generation.
FRONTIERS IN MEDICINE
(2023)
Review
Critical Care Medicine
Jessica C. Cardenas
Summary: Thrombin plays a central role in coagulation, essential for normal hemostasis. The generation of thrombin in severely injured patients is closely related to the risks of bleeding and thrombotic complications, and understanding the determinants of its production is clinically important.
Article
Medicine, General & Internal
Takeshi Matsumoto, Hideo Wada, Katsuya Shiraki, Kei Suzuki, Yoshiki Yamashita, Isao Tawara, Hideto Shimpo, Motomu Shimaoka
Summary: This study investigates the feasibility of evaluating coagulation ability using CWA-TT method in different hemophilia treatments. The results show that patients treated with FVIII concentrate exhibit hypercoagulability, while patients treated with Emicizumab do not show hypercoagulability.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Cell Biology
Ekaterina M. Koltsova, Anna D. Kuprash, Natalya M. Dashkevich, David M. Vardanyan, Artem Chernyakov, Maria A. Kumskova, Sukesh C. Nair, Alok Srivastava, Fazoil Ataullakhanov, Mikhail A. Panteleev, Anna N. Balandina
Summary: Thrombodynamics-4D is a new method for assessing the roles of plasma and platelets in clotting, allowing for the determination of plasma or platelet disorders and the effects of drugs on these processes. The assay involves clot formation in platelet-rich plasma or platelet-free plasma, activation with tissue factor, and registration of spatial fibrin clot growth and thrombin concentration dynamics through light scattering and fluorescence measurements.
Article
Hematology
Vijay K. Sonkar, Alicia S. Eustes, Azaj Ahmed, Melissa Jensen, Mitali V. Solanki, Jagadish Swamy, Rahul Kumar, Trevor P. Fidler, Jon C. D. Houtman, Bryan G. Allen, Douglas R. Spitz, E. Dale Abel, Sanjana Dayal
Summary: Reactive oxygen species (ROS) contribute to platelet hyperactivation during aging. In this study, it was found that mitochondria are an important source of platelet ROS and that mitochondrial SOD2 protects against mitochondrial ROS-driven platelet activation and thrombosis during aging.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Sandra Konrath, Reiner K. Mailer, Manu Beerens, Hanna Englert, Maike Frye, Piotr Kuta, Roger J. S. Preston, Coen Maas, Lynn M. Butler, Mark Roest, Bas de Laat, Thomas Renne
Summary: Calibrated Automated Thrombography (CAT) is a versatile and sensitive method for analyzing thrombin generation. In this study, a CAT method for analyzing thrombin generation in murine whole blood was developed, and different activators were used to stimulate thrombin generation in murine blood. The results showed that this method could be used to evaluate the process of coagulation activation.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Boaz Elad, Gilat Avraham, Naama Schwartz, Adi Elias, Mazen Elias
Summary: Thrombin's role in sepsis pathophysiology is central, and thrombin generation (TG) assays may be valuable in predicting infection severity and prognosis. Results showed that patients with higher qSOFA and SOFA scores had longer lag time and time to peak in thrombin generation, and those who died had lower ETP and peak height values.
SCIENTIFIC REPORTS
(2021)
Article
Health Care Sciences & Services
Liliana Vecerzan, Ariela Olteanu, Ionela Maniu, Adrian Boicean, Calin Remus Cipaian, Horatiu Dura, Sorin Radu Fleca, Romeo Gabriel Mihaila
Summary: This study analyzed thrombin generation in patients with chronic liver disease and found that it was lower compared to controls, decreasing as the disease progressed. These findings suggest that thrombin generation assays could help identify patients at high thrombotic and hemorrhagic risk and guide personalized treatment.
Article
Hematology
Marisa Ninivaggi, Romy de Laat-Kremers, Armando Tripodi, Denis Wahl, Stephane Zuily, Yesim Dargaud, Hugo ten Cate, Vera Ignjatovic, Katrien M. J. Devreese, Bas de Laat
Summary: Thrombin generation (TG) assay is a comprehensive tool for assessing hemostatic system functionality, but lack of standardization in its execution leads to poor correlation between test results. Recommendations based on survey and literature review address interlaboratory variation, aiming to harmonize methods and laboratories for supporting patient diagnosis and management with TG.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Medical Laboratory Technology
Fiston Kasonga, Guillaume Feugray, Pierre Chamouni, Virginie Barbay, Marielle Fresel, Marie Helene Chretien, Sabine Brunel, Veronique Le Cam Duchez, Paul Billoir
Summary: This study aimed to assess whether thrombin generation assay (TGA) could be used to evaluate the hemorrhagic phenotype of patients with FXI deficiency. The results showed a slight decrease in thrombin generation in plasma samples of patients with FXI deficiency, while coagulation parameters increased during pregnancy in women with FXI deficiency.
CLINICA CHIMICA ACTA
(2021)
Article
Hematology
Claire de Moreuil, Yesim Dargaud, Christophe Nougier, Pierre-Francois Dupr, Christoph Tremouilhac, Delphine Le Joliff, Sylvain Rosec, Sandy Lucier, Ingrid Pabinger, Cihan Ay, Francis Couturaud, Brigitte Pan-Petesch
Summary: This study aimed to characterize the coagulation properties of predelivery plasmas from pregnant women with severe postpartum hemorrhage (PPH). Thrombin generation assay and hemostatic biomarkers were used to measure these properties. The results suggest that predelivery endogenous thrombin generation potential (ETP) levels may have predictive value for severe PPH.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Immunology
Qiuting Yan, Shengshi Huang, Wouter van der Heijden, Marisa Ninivaggi, Lisa van de Wijer, Romy de Laat-Kremers, Andre J. van der Ven, Bas de Laat, Quirijn de Mast
Summary: This study further explores the relationship between abacavir (ABC) use and thrombin generation through thrombin dynamics analysis, and finds that the association between ABC use and thrombin generation may be due to an increase in the prothrombin conversion pathway. In a cross-sectional study of 208 virally suppressed PLHIV, it is found that the total amount and maximum rate of prothrombin conversion are significantly increased in PLHIV on an ABC-containing regimen, indicating that ABC affects the kinetics of prothrombin conversion rather than procoagulant factor levels.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Hematology
Takumi Tsuchida, Mineji Hayakawa, Shota Kawahara, Osamu Kumano
Summary: This study aimed to investigate the effects of antithrombin (AT) deficiency on coagulation balance using a thrombin generation assay. The results showed that the ability to generate thrombin increased as AT activity decreased, and thrombin generation was significantly suppressed when AT activity was equal to or below 50%. Particularly, thrombin generation was remarkable when AT activity was below 30%, indicating a poor prognosis due to organ failure from thrombotic tendency.
THROMBOSIS JOURNAL
(2022)
Letter
Medical Laboratory Technology
Giuseppe Lippi, Chiara Bovo, Gian Luca Salvagno
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2020)
Article
Medical Laboratory Technology
Giuseppe Lippi, Gian Luca Salvagno, Matteo Gelati, Mairi Pucci, Claudia Lo Cascio, Davide Demonte, Diego Faggian, Mario Plebani
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2020)
Article
Endocrinology & Metabolism
A. Mantovani, T. Turino, M. G. Lando, K. Gjini, C. D. Byrne, C. Zusi, F. Ravaioli, A. Colecchia, C. Maffeis, G. Salvagno, G. Lippi, E. Bonora, G. Targher
DIABETES & METABOLISM
(2020)
Article
Cell Biology
L. Dalle Carbonare, M. Mottes, S. Cheri, M. Deiana, F. Zamboni, D. Gabbiani, F. Schena, G. L. Salvagno, G. Lippi, M. T. Valenti
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2019)
Article
Endocrinology & Metabolism
Flavia Tosi, Francesca Dal Molin, Federica Zamboni, Enrica Saggiorato, Gian Luca Salvagno, Tom Fiers, Jean-Marc Kaufman, Enzo Bonora, Paolo Moghetti
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2020)
Article
Medicine, General & Internal
Giuseppe Lippi, Gian Luca Salvagno, Matteo Gelati, Mairi Pucci, Davide Demonte, Diego Faggian, Mario Plebani
Article
Medical Laboratory Technology
Cantor Tarperi, Fabian Sanchis-Gomar, Martina Montagnana, Elisa Danese, Gian Luca Salvagno, Matteo Gelati, Kristina Skroce, Federico Schena, Giuseppe Lippi
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2020)
Letter
Medical Laboratory Technology
Giuseppe Lippi, Gian Luca Salvagno, Manuela Pegoraro, Valentina Militello, Cecilia Caloi, Angelo Peretti, Stefania Gaino, Antonella Bassi, Chiara Bovo, Giuliana Lo Cascio
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2020)
Letter
Medical Laboratory Technology
Giuseppe Lippi, Gian Luca Salvagno, Manuela Pegoraro, Valentina Militello, Cecilia Caloi, Angelo Peretti, Simone De Nitto, Chiara Bovo, Giuliana Lo Cascio
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2020)
Article
Medical Laboratory Technology
Gian Luca Salvagno, Elisa Danese, Giuseppe Lippi
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2020)
Article
Endocrinology & Metabolism
Paolo Cavarzere, Rossella Gaudino, Marco Sandri, Diego Alberto Ramaroli, Angelo Pietrobelli, Marco Zaffanello, Alessandra Guzzo, Gian Luca Salvagno, Giorgio Piacentini, Franco Antoniazzi
EUROPEAN JOURNAL OF ENDOCRINOLOGY
(2020)
Article
Endocrinology & Metabolism
A. Mantovani, E. Danese, G. L. Salvagno, M. Gelati, T. Turino, C. Bovo, G. Lippi, G. Targher
Summary: The study found a significant association between lower plasma adiponectin levels and increased plasma thrombin generation in men with non-insulin-treated T2DM, mainly mediated by plasma triglycerides.
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
(2021)
Letter
Hematology
Nicola Martinelli, Martina Montagnana, Francesca Pizzolo, Simonetta Friso, Gian Luca Salvagno, Gian Luca Forni, Barbara Gianesin, Matteo Morandi, Claudio Lunardi, Giuseppe Lippi, Enrico Polati, Oliviero Olivieri, Lucia De Franceschi
THROMBOSIS RESEARCH
(2020)
Article
Oncology
Gian Luca Salvagno, Davide Demonte, Francesco Dima, Chiara Bovo, Giuseppe Lippi
HEMATOLOGY TRANSFUSION AND CELL THERAPY
(2020)
Article
Biochemistry & Molecular Biology
Gian Luca Salvagno, Davide Demonte, Matteo Gelati, Giovanni Poli, Emmanuel J. Favaloro, Giuseppe Lippi
JOURNAL OF MEDICAL BIOCHEMISTRY
(2020)