Article
Chemistry, Medicinal
Brittney J. Mills, Malika P. Godamudunage, Siyuan Ren, Malabika Laha
Summary: This study evaluated the biophysical properties of two ADCs in 16 formulation conditions using high-throughput techniques and verified the predictive capabilities of the screening methods through stability studies. The results showed that high-throughput stability indicators, such as Tagg and B22, have similar ranking abilities as traditional methods like SEC data in assessing aggregate formation, while conformational stability indicators (Tm) are less predictive. Additionally, the high-throughput assays effectively identified poor performing formulation conditions, which is the ultimate goal of screening assays.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Review
Optics
Xurong Li, Jingxi Li, Yuhang Li, Aydogan Ozcan, Mona Jarrahi
Summary: This article reviews the development of terahertz imaging technologies and discusses different types of hardware and computational imaging algorithms. It explores opportunities for capturing various image data and briefly introduces the prospects and challenges for future high-throughput terahertz imaging systems.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Biology
Srikanya Kundu, Molly E. Boutin, Caroline E. Strong, Ty Voss, Marc Ferrer
Summary: Fibrin gel-based 3D co-culture models with human-iPSC differentiated neurons and astrocytes, combined with biosensors and optogenetics activation, enable the creation of functional neural models for drug response studies.
COMMUNICATIONS BIOLOGY
(2022)
Review
Immunology
Thomas Samuel Ram, Vinotha Thomas, Arvind Sathyamurthy, Jeba Karunya Ramireddy, Neenu Oliver John, Anitha Thomas, Abraham Peedicayil
Summary: The global increase in cancer burden poses a challenge for countries with scarce resources, with gynecological cancer remaining prevalent. Training gynecologic oncologists is crucial in decreasing morbidity and mortality rates, with challenges and opportunities existing in the development of training programs, particularly in developing countries like India.
INDIAN JOURNAL OF MEDICAL RESEARCH
(2021)
Review
Oncology
Bo Qiu, Cassie Kline, Sabine Mueller
Summary: Pediatric brain tumors are the most common solid tumors in children, with some high-risk diagnoses remaining incurable. There is ongoing development of therapy options for CNS tumors, but for most high-risk patients, single agent treatments may not be sufficient.
FRONTIERS IN ONCOLOGY
(2021)
Review
Food Science & Technology
Regine Eibl, Yannick Senn, Geraldine Gubser, Valentin Jossen, Christian van den Bos, Dieter Eibl
Summary: Cellular agriculture is a new technology that produces agricultural products using cells and tissues, providing a variety of foods and materials to meet the growing population demand while conserving important resources. Despite early successes, challenges remain in the field.
ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 12, 2021
(2021)
Review
Chemistry, Multidisciplinary
Aurelie Lacroix, Hanadi F. Sleiman
Summary: Despite the programmable assembly and precise control of size, shape, and function offered by DNA nanotechnology, challenges such as enzymatic hydrolysis, low cellular uptake, immune cell recognition and degradation, and unclear biodistribution profiles still need to be addressed before utilizing DNA particles in biological applications. Rigorous methodologies are necessary to study, understand, and control the fate of self-assembled DNA structures under physiological conditions. Achieving a detailed understanding of the fate of DNA nanostructures within living organisms through thorough characterization is crucial for driving the translation of DNA constructs toward preclinical design and clinical maturity.
Article
Biochemical Research Methods
Celine Rens, Tirosh Shapira, Sandra Pena-Diaz, Joseph D. Chao, Tom Pfeifer, Yossef Av-Gay
Summary: The authors developed AUTOptosis, a cost-effective and rapid apoptosis monitoring method based on quantifying nuclei intensity via staining with Hoechst 33342. They calibrated the method with standard apoptosis inducers in multiple cell lines, validated its applicability for high-content screening using a compound library, and demonstrated its specificity by detecting apoptosis triggered by Mycobacterium tuberculosis infections. AUTOptosis method involves morphological analysis of nuclear condensation during caspase-3-dependent apoptosis, where Hoechst-stained nuclei appear brighter during apoptosis and fade during necrosis, providing an apoptosis index for comparing test and control conditions.
Review
Physics, Applied
Wensi Cai, Haiyun Li, Mengchao Li, Meng Wang, Huaxin Wang, Jiangzhao Chen, Zhigang Zang
Summary: Inorganic perovskite-based photodetectors have attracted considerable attention due to their increased stability and exceptional optoelectronic properties, showing great potential in various applications. Progress has been made in the versatile compositions and morphologies of constituent materials, diverse device architectures, and coverage of sensing regions from UV-visible-NIR to x-ray and even gamma-ray. However, current challenges hindering the commercialization of these photodetectors are also highlighted, with possible solutions provided.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2021)
Article
Biochemical Research Methods
Gregory J. Hunt, Mark A. Dane, James E. Korkola, Laura M. Heiser, Johann A. Gagnon-Bartsch
Summary: In this study, we propose a general approach that leverages an experimental design with multiple levels of replication to remove technical artifacts from high-throughput image data. By applying this approach to microenvironment microarrays (MEMAs), we are able to eliminate unwanted spatial artifacts and enhance the biological signal. This method has broad applicability in various biological assays.
Review
Genetics & Heredity
Bin Gu, Maxwell C. Hakun
Summary: NUT carcinoma is an aggressive cancer driven by chromosome translocations, with fusion genes being a key factor in its development. Despite 20 years of research, challenging questions remain regarding the genetic etiology and therapeutic development of NC. Advances in genome editing, animal models, stem cell biology, organoids, and chemical biology present unique opportunities to address these challenges.
Article
Multidisciplinary Sciences
Jayme L. Dahlin, Bruce K. Hua, Beth E. Zucconi, Shawn D. Nelson, Shantanu Singh, Anne E. Carpenter, Jonathan H. Shrimp, Evelyne Lima-Fernandes, Mathias J. Wawer, Lawrence P. W. Chung, Ayushi Agrawal, Mary O'Reilly, Dalia Barsyte-Lovejoy, Magdalena Szewczyk, Fengling Li, Parnian Lak, Matthew Cuellar, Philip A. Cole, Jordan L. Meier, Tim Thomas, Jonathan B. Baell, Peter J. Brown, Michael A. Walters, Paul A. Clemons, Stuart L. Schreiber, Bridget K. Wagner
Summary: Efficiently identifying compounds with undesirable mechanisms of action in complex cellular assays is still challenging. In this study, the authors use cell painting and cellular health profiles to characterize the cellular morphologies associated with compound-dependent cellular injury and nuisance activity. They show that a variety of compounds causing cellular damage produce bioactive cell painting morphologies, and propose the inclusion of cytotoxic and nuisance reference compounds for assay optimization and compound prioritization in complex cellular assays.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Md Mahadhi Hasan, Lotten Ragnarsson, Fernanda C. Cardoso, Richard J. Lewis
Summary: Chemical transfection methods including calcium phosphate, FuGENE, and Lipofectamine 3000 were compared for transient expression of voltage-gated ion channels Ca(V)2.2 and Na(V)1.7. Results indicated that FuGENE-mediated transfection was the most effective for high-throughput automated patch clamp assays.
Article
Medicine, General & Internal
George Semeniuk, Bahareh Bahadini, Eugene Ahn, Jasmine Zain, Jessica Cheng, Ameish Govindarajan, Judy Rose, Richard T. Lee
Summary: Integrative oncology is an emerging field that combines conventional cancer treatments with integrative therapies to improve quality of life for cancer patients. Clinical research has shown the benefits of these approaches in symptom management and quality of life, leading to their inclusion in national guidelines.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Immunology
Sarah Rumler
Summary: This article discusses the immune therapies for pediatric CNS tumors, including checkpoint inhibitors, vaccines, radiation therapy, and early studies of antibody-mediated immunogenic therapies. The article also highlights the importance of combination immunotherapy and addresses the neurologic toxicities associated with immunotherapies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Feng-Ming (Spring) Kong, Vitali Moiseenko, Jing Zhao, Michael T. Milano, Ling Li, Andreas Rimner, Shiva Das, X. Allen Li, Moyed Miften, ZhongXing Liao, Mary Martel, Soren M. Bentzen, Andrew Jackson, Jimm Grimm, Lawrence B. Marks, Ellen Yorke
Summary: SBRT has become the standard of care for inoperable early-stage non-small cell lung cancer, recurrent lung cancer, and pulmonary metastases. Risk of RILT, including radiation pneumonitis and pulmonary fibrosis, is associated with tumor size and location, and patients with interstitial lung disease appear to be especially susceptible. Safe treatment with a low rate of symptomatic RILT can be achieved by controlling the mean lung dose and the percentage of total lung volume receiving high radiation doses.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2021)
Article
Hematology
Blake S. Moses, Samantha McCullough, Jennifer M. Fox, Bryan T. Mott, Soren M. Bentzen, MinJung Kim, Jeffrey W. Tyner, Rena G. Lapidus, Ashkan Emadi, Michelle A. Rudek, Tami J. Kingsbury, Curt Civin
Summary: Artemisinins have shown synergy with BCL2 inhibitors in inhibiting leukemia cell growth and inducing apoptosis. The SAV regimen targets MCL1 and BCL2 through tolerable mechanisms, with MCL1 and DDIT3/CHOP levels potentially serving as biomarkers for the action of artemisinins and SAV.
Article
Oncology
Maria R. Baer, Aksinija A. Kogan, Soren M. Bentzen, Tian Mi, Rena G. Lapidus, Vu H. Duong, Ashkan Emadi, Sandrine Niyongere, Casey L. O'Connell, Benjamin A. Youngblood, Stephen B. Baylin, Feyruz Rassool
Summary: This study reports a phase I clinical trial combining the DNMTi decitabine and the PARPi talazoparib in relapsed/refractory AML. The combination therapy was well tolerated and demonstrated the expected pharmacodynamic effects in patients.
CLINICAL CANCER RESEARCH
(2022)
Article
Oncology
Matthew C. Ward, Soren M. Bentzen, Carolina E. Fasola, Atif J. Khan, Richard L. White Jr, Frank Vicini, Chirag Shah
Summary: The study estimated the risk of local failure in early-stage breast cancer patients who received postoperative radiation therapy. The results showed that the risk of local failure was slightly higher with the intraoperative 50 kV x-rays combined with postoperative radiation therapy compared to external beam radiation therapy, and relatively higher with the TARGIT strategy alone.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2023)
Letter
Oncology
Ivan R. Vogelius, Soren M. Bentzen
Article
Infectious Diseases
Rebecca G. Nowak, Soren M. Bentzen, Lisa M. Schumaker, Nicholas P. Ambulos, Nicaise Ndembi, Wuese Dauda, Andrew Mitchell, Trevor J. Mathias, Trevor A. Crowell, Stefan D. Baral, William A. Blattner, Manhattan E. Charurat, Joel M. Palefsky, Kevin J. Cullen
Summary: Multiple anal human papillomavirus (HPVs) may increase the risk of anal cancer among men who have sex with men (MSM) living with human immunodeficiency virus (HIV). This study explored the Jaccard Similarity Index (JSI) as a quantitative measure of multiple HPV persistence and found that highly similar HPV genotypes were associated with HIV infection and recent receptive sex. Further research is needed to evaluate the JSI as a predictor of high-grade intraepithelial lesions.
SEXUALLY TRANSMITTED DISEASES
(2022)
Article
Oncology
Dahlia Kronfli, Akilah Lievers, Kaysee Baker, Caitlin Eggleston, Robert Miller, Soren M. Bentzen, Pranshu Mohindra, Melissa A. L. Vyfhuis
Summary: This study analyzed the psychosocial needs of cancer patients undergoing radiation therapy using a questionnaire. The study found that Black patients had different characteristics compared to non-Black patients in terms of marital status, income, and treatment location. Additionally, Black patients had higher rates of unmet needs regarding pain, stress management, transportation, and smoking cessation.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2022)
Article
Oncology
Bianca A. W. Hoeben, Montserrat Pazos, Enrica Seravalli, Mirjam E. Bosman, Christoph Losert, Michael H. Albert, Tom Boterberg, Inna Ospovat, Soraya Mico Milla, Candan Demiroz Abakay, Jacob Engellau, Vilberg Johannesson, Gregor Kos, Stephane Supiot, Camille Llagostera, Marc Bierings, Giovanni Scarzello, Klaus Seiersen, Ed Smith, Abrahams Ocanto, Carlos Ferrer, Soren M. Bentzen, Daria A. Kobyzeva, Anna A. Loginova, Geert O. Janssens
Summary: This study summarizes the techniques and toxicities of myeloablative TBI in children and provides consensus recommendations. TBI is mainly used as conditioning for high-risk ALL or relapsed hematologic malignancies in HSCT. Dose limitations for different organs are proposed. In addition, highly conformal TBI techniques are also worth further research.
RADIOTHERAPY AND ONCOLOGY
(2022)
Article
Oncology
Rahul Khairnar, Lyudmila DeMora, Howard M. Sandler, W. Robert Lee, Ester Villalonga-Olives, C. Daniel Mullins, Francis B. Palumbo, Deborah W. Bruner, Fadia T. Shaya, Soren M. Bentzen, Amit B. Shah, Shawn Malone, Jeff M. Michalski, Ian S. Dayes, Samantha A. Seaward, Michele Albert, Adam D. Currey, Thomas M. Pisansky, Yuhchyau Chen, Eric M. Horwitz, Albert S. DeNittis, Felix Feng, Mark V. Mishra
Summary: The study aimed to compare the predictive ability of mapping algorithms derived from cross-sectional and longitudinal data. By using data from a trial of patients with low-risk prostate cancer, researchers found that models obtained from baseline cross-sectional data showed the best predictive performance. Models using only EPIC domain/subdomain data had better predictive ability when combined data was used, while models using baseline data outperformed others when patient covariates were considered.
JCO CLINICAL CANCER INFORMATICS
(2022)
Article
Oncology
Matthew P. Deek, Kim Van der Eecken, Philip Sutera, Rebecca A. Deek, Valerie Fonteyne, Adrianna A. Mendes, Karel Decaestecker, Ana Ponce Kiess, Nicolaas Lumen, Ryan Phillips, Aurelie De Bruycker, Mark Mishra, Zaker Rana, Jason Molitoris, Bieke Lambert, Louke Delrue, Hailun Wang, Kathryn Lowe, Sofie Verbeke, Jo Van Dorpe, Renee Bultijnck, Geert Villeirs, Kathia De Man, Filip Ameye, Daniel Y. Song, Theodore DeWeese, Channing J. Paller, Felix Y. Feng, Alexander Wyatt, Kenneth J. Pienta, Maximillian Diehn, Soren M. Bentzen, Steven Joniau, Friedl Vanhaverbeke, Gert De Meerleer, Emmanuel S. Antonarakis, Tamara L. Lotan, Alejandro Berlin, Shankar Siva, Piet Ost, Phuoc T. Tran
Summary: This study presents the long-term outcomes of metastasis-directed therapy (MDT) in oligometastatic castration-sensitive prostate cancer (omCSPC) and investigates the impact of high-risk mutations on treatment outcomes.
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Letter
Oncology
Cem Onal, Duygu Sezen, Ezgi Oymak, Yasemin Bolukbasi
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2023)
Article
Oncology
Bansi Savla, M. A. Hamza, D. Yacubovich, S. Cobbs, L. Petrovska, K. A. Scilla, W. Burrows, R. Mehra, R. C. Miller, C. Rolfo, S. M. Bentzen, P. Mohindra, Melissa A. L. Vyfhuis
Summary: This study aimed to analyze the impact of living in food priority areas on treatment and cancer outcomes in patients with locally advanced non-small cell lung cancer (NSCLC). The results showed that patients living in FPAs were more likely to receive less-aggressive therapy, and residing in an FPA resulted in poorer outcomes for patients with a normal-weight BMI.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2023)
Article
Genetics & Heredity
Edward C. So, Hua Zhou, Ariana Greenwell, Erin E. Burch, Yaping Ji, Emmanuel Y. Merigeon, Henrik S. Olsen, Soren M. Bentzen, David S. Block, Xiaoyu Zhang, Scott E. Strome
Summary: This study investigates the influence of C1q decoration on immune complexes (ICs) and their interactions with FcyRs. The results show that C1q engagement directly and transiently inhibits the interactions between ICs and FcyRIII on NK cells. The inhibitory effect is associated with IC size and the concentrations of both C1q and Fc multimers. Functionally, C1q-mediated Fc blockade limits the ability of NK cells to induce the upregulation of CD137 and mediate ADCC.
Editorial Material
Oncology
Soren M. Bentzen, Ivan R. Vogelius
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2023)
Article
Oncology
Line Bjerregaard Stick, Maria Fuglsang Jensen, Soren M. Bentzen, Claus Kamby, Anni Young Lundgaard, Maja Vestmo Maraldo, Birgitte Vrou Offersen, Jen Yu, Ivan Richter Vogelius
Summary: This study compared photon and proton therapy plans for patients with synchronous bilateral early breast cancer and estimated risks of early and late radiation-induced toxicities. The results showed that proton therapy plans improved target coverage and reduced the risk of coronary artery events and secondary lung cancer compared to photon therapy, but increased the risk of radiation dermatitis.
INTERNATIONAL JOURNAL OF PARTICLE THERAPY
(2022)